Search results for: Kazuo Mori
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 27

Search results for: Kazuo Mori

27 The Effect of Contrived Success in Calculation Tasks on the Self-efficacy of Junior High School Students

Authors: Akitoshi Uchida, Kazuo Mori

Abstract:

This study examines whether contrived success on a task closely related to school subjects would promote students- self-efficacy. In our previous study, junior high school students who experienced contrived success on anagram tasks raised their sense of self-efficacy and kept it high for a year.We tried to replicate that study, substituting calculation tasks for the anagrams. One hundred eighteen junior high school students participated in this study, 18 of whom were surreptitiously given easier tasks than their classmates. Those students with easier tasks outperformed their peers and thereby raised their sense of self-efficacy. However, elevated self-efficacy did not persist, falling to the starting level after only three months.

Keywords: self-efficacy, contrived success, junior high schoolstudents, calculation tasks

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26 Multihop Cooperative Transmissions for Asymmetric Traffic Accommodation in CDMA/FDD Cellular Networks

Authors: Kazuo Mori, Takeo Saga, Katsuhiro Naito, Hideo Kobayashi

Abstract:

The asymmetric trafc between uplink and downlink over recent mobile communication systems has been conspicuous because of providing new communication services. This paper proposes an asymmetric trafc accommodation scheme adopting a multihop cooperative transmission technique for CDMA/FDD cellular networks. The proposed scheme employs the cooperative transmission technique in the already proposed downlink multihop transmissions for the accommodation of the asymmetric trafc, which utilizes the vacant uplink band for the downlink relay transmissions. The proposed scheme reduces the transmission power at the downlink relay transmissions and then suppresses the interference to the uplink communications, and thus, improves the uplink performance. The proposed scheme is evaluated by computer simulation and the results show that it can achieve better throughput performance.

Keywords: asymmetric traffic, cooperative transmissions, multihop transmissions, CDMA, FDD, cellular systems

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25 Making a ‘Once-upon-a-Time’ Mythology in Kazuo Ishiguro’s The Buried Giant

Authors: Masami Usui

Abstract:

Kazuo Ishiguro’s challenging novel, The Buried Giant, embodies how contemporary writers and readers have to discover the voices buried in our history. By avoiding setting or connecting the modern and contemporary historical incidents such as World War II this time, Ishiguro ventures into retelling myth, transfiguring historical facts, and revealing what has been forgotten in a process of establishing history and creating mythology. As generally known, modernist writers in the twentieth century employed materials from authorized classical mythologies, especially Greek mythology. As an heir of this tradition, Ishiguro imposes his mission of criticizing the repeatedly occurring yet easily-forgotten history of dictatorship and a slaughter on mythology based on King Arthur and its related heroes and myths in Britain. On an open ground, Ishiguro can start his own mythical story and space.

Keywords: English literature, fantasy, global literature, mythology.

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24 Two-Stage Compensator Designs with Partial Feedbacks

Authors: Kazuyoshi MORI

Abstract:

The two-stage compensator designs of linear system are investigated in the framework of the factorization approach. First, we give “full feedback" two-stage compensator design. Based on this result, various types of the two-stage compensator designs with partial feedbacks are derived.

Keywords: Linear System, Factorization Approach, Two-Stage Compensator Design, Parametrization of Stabilizing Controllers.

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23 The Locker Problem with Empty Lockers

Authors: David Avis, Luc Devroye, Kazuo Iwama

Abstract:

We consider a cooperative game played by n players against a referee. The players names are randomly distributed among n lockers, with one name per locker. Each player can open up to half the lockers and each player must find his name. Once the game starts the players may not communicate. It has been previously shown that, quite surprisingly, an optimal strategy exists for which the success probability is never worse than 1 − ln 2 ≈ 0.306. In this paper we consider an extension where the number of lockers is greater than the number of players, so that some lockers are empty. We show that the players may still win with positive probability even if there are a constant k number of empty lockers. We show that for each fixed probability p, there is a constant c so that the players can win with probability at least p if they are allowed to open cn lockers.

Keywords: Locker problem, pointer-following algorithms.

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22 The Importance of 3D Mesh Generation for Large Eddy Simulation of Gas – Solid Turbulent Flows in a Fluidized Beds

Authors: G. González-Silva, E. M. Matos, W. P. Martignoni, M. Mori

Abstract:

The objective of this work is to show a procedure for mesh generation in a fluidized bed using large eddy simulations (LES) of a filtered two-fluid model. The experimental data were obtained by [1] in a laboratory fluidized bed. Results show that it is possible to use mesh with less cells as compared to RANS turbulence model with granular kinetic theory flow (KTGF). Also, the numerical results validate the experimental data near wall of the bed, which cannot be predicted by RANS.model.

Keywords: LES, Mesh, Gas-Solid, Fluidized bed

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21 Design of Nonlinear Observer by Using Chebyshev Interpolation based on Formal Linearization

Authors: Kazuo Komatsu, Hitoshi Takata

Abstract:

This paper discusses a design of nonlinear observer by a formal linearization method using an application of Chebyshev Interpolation in order to facilitate processes for synthesizing a nonlinear observer and to improve the precision of linearization. A dynamic nonlinear system is linearized with respect to a linearization function, and a measurement equation is transformed into an augmented linear one by the formal linearization method which is based on Chebyshev interpolation. To the linearized system, a linear estimation theory is applied and a nonlinear observer is derived. To show effectiveness of the observer design, numerical experiments are illustrated and they indicate that the design shows remarkable performances for nonlinear systems.

Keywords: nonlinear system, nonlinear observer, formal linearization, Chebyshev interpolation.

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20 A Relationship between Two Stabilizing Controllers and Its Application to Two-Stage Compensator Design without Coprime Factorizability – Single-Input Single-Output Case –

Authors: Kazuyoshi Mori

Abstract:

In this paper, we first show a relationship between two stabilizing controllers, which presents an extended feedback system using two stabilizing controllers. Then, we apply this relationship to the two-stage compensator design. In this paper, we consider singleinput single-output plants. On the other hand, we do not assume the coprime factorizability of the model. Thus, the results of this paper are based on the factorization approach only, so that they can be applied to numerous linear systems.

Keywords: Relationship among Compensators, Two-Stage Compensator Design, Parametrization of Stabilizing Controllers, Factorization Approach

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19 An Approach to Control Design for Nonlinear Systems via Two-stage Formal Linearization and Two-type LQ Controls

Authors: Kazuo Komatsu, Hitoshi Takata

Abstract:

In this paper we consider a nonlinear control design for nonlinear systems by using two-stage formal linearization and twotype LQ controls. The ordinary LQ control is designed on almost linear region around the steady state point. On the other region, another control is derived as follows. This derivation is based on coordinate transformation twice with respect to linearization functions which are defined by polynomials. The linearized systems can be made up by using Taylor expansion considered up to the higher order. To the resulting formal linear system, the LQ control theory is applied to obtain another LQ control. Finally these two-type LQ controls are smoothly united to form a single nonlinear control. Numerical experiments indicate that this control show remarkable performances for a nonlinear system.

Keywords: Formal Linearization, LQ Control, Nonlinear Control, Taylor Expansion, Zero Function.

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18 Number of Parametrization of Discrete-Time Systems without Unit-Delay Element: Single-Input Single-Output Case

Authors: Kazuyoshi Mori

Abstract:

In this paper, we consider the parametrization of the discrete-time systems without the unit-delay element within the framework of the factorization approach. In the parametrization, we investigate the number of required parameters. We consider single-input single-output systems in this paper. By the investigation, we find, on the discrete-time systems without the unit-delay element, three cases that are (1) there exist plants which require only one parameter and (2) two parameters, and (3) the number of parameters is at most three.

Keywords: Linear systems, parametrization, Coprime Factorization, number of parameters.

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17 Design of Nonlinear Observer by Using Augmented Linear System based on Formal Linearization of Polynomial Type

Authors: Kazuo Komatsu, Hitoshi Takata

Abstract:

The objective of this study is to propose an observer design for nonlinear systems by using an augmented linear system derived by application of a formal linearization method. A given nonlinear differential equation is linearized by the formal linearization method which is based on Taylor expansion considering up to the higher order terms, and a measurement equation is transformed into an augmented linear one. To this augmented dimensional linear system, a linear estimation theory is applied and a nonlinear observer is derived. As an application of this method, an estimation problem of transient state of electric power systems is studied, and its numerical experiments indicate that this observer design shows remarkable performances for nonlinear systems.

Keywords: nonlinear system, augmented linear system, nonlinear observer, formal linearization, electric power system.

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16 3-D Visualization and Optimization for SISO Linear Systems Using Parametrization of Two-Stage Compensator Design

Authors: Kazuyoshi Mori, Keisuke Hashimoto

Abstract:

In this paper, we consider the two-stage compensator designs of SISO plants. As an investigation of the characteristics of the two-stage compensator designs, which is not well investigated yet, of SISO plants, we implement three dimensional visualization systems of output signals and optimization system for SISO plants by the parametrization of stabilizing controllers based on the two-stage compensator design. The system runs on Mathematica by using “Three Dimensional Surface Plots,” so that the visualization can be interactively manipulated by users. In this paper, we use the discrete-time LTI system model. Even so, our approach is the factorization approach, so that the result can be applied to many linear models.

Keywords: Linear systems, visualization, optimization, two-Stage compensator design, Mathematica.

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15 Electrical Characteristics of Biomodified Electrodes using Nonfaradaic Electrochemical Impedance Spectroscopy

Authors: Yusmeeraz Yusof, Yoshiyuki Yanagimoto, Shigeyasu Uno, Kazuo Nakazato

Abstract:

We demonstrate a nonfaradaic electrochemical impedance spectroscopy measurement of biochemically modified gold plated electrodes using a two-electrode system. The absence of any redox indicator in the impedance measurements provide more precise and accurate characterization of the measured bioanalyte at molecular resolution. An equivalent electrical circuit of the electrodeelectrolyte interface was deduced from the observed impedance data of saline solution at low and high concentrations. The detection of biomolecular interactions was fundamentally correlated to electrical double-layer variation at modified interface. The investigations were done using 20mer deoxyribonucleic acid (DNA) strands without any label. Surface modification was performed by creating mixed monolayer of the thiol-modified single-stranded DNA and a spacer thiol (mercaptohexanol) by a two-step self-assembly method. The results clearly distinguish between the noncomplementary and complementary hybridization of DNA, at low frequency region below several hundreds Hertz.

Keywords: Biosensor, electrical double-layer, impedance spectroscopy, label free DNA.

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14 Interbank Networks and the Benefits of Using Multilayer Structures

Authors: Danielle Sandler dos Passos, Helder Coelho, Flávia Mori Sarti

Abstract:

Complexity science seeks the understanding of systems adopting diverse theories from various areas. Network analysis has been gaining space and credibility, namely with the biological, social and economic systems. Significant part of the literature focuses only monolayer representations of connections among agents considering one level of their relationships, and excludes other levels of interactions, leading to simplistic results in network analysis. Therefore, this work aims to demonstrate the advantages of the use of multilayer networks for the representation and analysis of networks. For this, we analyzed an interbank network, composed of 42 banks, comparing the centrality measures of the agents (degree and PageRank) resulting from each method (monolayer x multilayer). This proved to be the most reliable and efficient the multilayer analysis for the study of the current networks and highlighted JP Morgan and Deutsche Bank as the most important banks of the analyzed network.

Keywords: Complexity, interbank networks, multilayer networks, network analysis.

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13 Electrospinning and Characterization of Silk Fibroin/Gelatin Nanofibre Mats

Authors: S. Mohammadzadehmoghadam, Y. Dong

Abstract:

In this study, Bombyx mori silk fibroin/gelatin (SF/GT) nanocomposite with different GT ratio (SF/GT 100/0, 90/10 and 70/30) were prepared by electrospinning process and crosslinked with glutaraldehyde (GA) vapor. Properties of crosslinked SF/GT nanocomposites were investigated by scanning electron microscopy (SEM), mechanical test, water uptake capacity (WUC) and porosity. From SEM images, it was found that fiber diameter increased as GT content increased. The results of mechanical test indicated that the SF/GT 70/30 nanocomposites had both the highest Young’s modulus of 342 MPa and the highest tensile strength of about 14 MPa. However, porosity and WUC decreased from 62% and 405% for pristine SF to 47% and 232% for SF/GT 70/30, respectively. This behavior can be related to higher degree of crosslinking as GT ratio increased which altered the structure and physical properties of scaffolds. This study showed that incorporation of GT into SF nanofibers can enhance mechanical properties of resultant nanocomposite, but the GA treatment should be optimized to control and fine-tune other properties to warrant their biomedical application.

Keywords: Electrospinning, gelatin, mechanical properties, nanocomposites, silk fibroin.

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12 Novel CFRP Adhesive Joints and Structures for Offshore Application

Authors: M. R. Abusrea, Shiyi Jiang, Dingding Chen, Kazuo Arakawa

Abstract:

Novel wind-lens turbine designs can augment power output. Vacuum-Assisted Resin Transfer Molding (VARTM) is used to form large and complex structures from a Carbon Fiber Reinforced Polymer (CFRP) composite. Typically, wind-lens turbine structures are fabricated in segments, and then bonded to form the final structure. This paper introduces five new adhesive joints, divided into two groups: one is constructed between dry carbon and CFRP fabrics, and the other is constructed with two dry carbon fibers. All joints and CFRP fabrics were made in our laboratory using VARTM manufacturing techniques. Specimens were prepared for tensile testing to measure joint performance. The results showed that the second group of joints achieved a higher tensile strength than the first group. On the other hand, the tensile fracture behavior of the two groups showed the same pattern of crack originating near the joint ends followed by crack propagation until fracture.

Keywords: Adhesive joints, CFRP, VARTM, resin transfer molding.

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11 Design of Extremum Seeking Control with PD Accelerator and its Application to Monod and Williams-Otto Models

Authors: Hitoshi Takata, Tomohiro Hachino, Masaki Horai, Kazuo Komatsu

Abstract:

In this paper, we are concerned with the design and its simulation studies of a modified extremum seeking control for nonlinear systems. A standard extremum seeking control has a simple structure, but it takes a long time to reach an optimal operating point. We consider a modification of the standard extremum seeking control which is aimed to reach the optimal operating point more speedily than the standard one. In the modification, PD acceleration term is added before an integrator making a principal control, so that it enables the objects to be regulated to the optimal point smoothly. This proposed method is applied to Monod and Williams-Otto models to investigate its effectiveness. Numerical simulation results show that this modified method can improve the time response to the optimal operating point more speedily than the standard one.

Keywords: Extremum seeking control, Monod model, Williams- Otto model, PD acceleration term, Optimal operating point.

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10 Application of Digital Image Correlation Technique on Vacuum Assisted Resin Transfer Molding Process and Performance Evaluation of the Produced Materials

Authors: Dingding Chen, Kazuo Arakawa, Masakazu Uchino, Changheng Xu

Abstract:

Vacuum assisted resin transfer moulding (VARTM) is a promising manufacture process for making large and complex fiber reinforced composite structures. However, the complexity of the flow of the resin in the infusion stage usually leads to nonuniform property distribution of the produced composite part. In order to control the flow of the resin, the situation of flow should be mastered. For the safety of the usage of the produced composite in practice, the understanding of the property distribution is essential. In this paper, we did some trials on monitoring the resin infusion stage and evaluation for the fiber volume fraction distribution of the VARTM produced composite using the digital image correlation methods. The results showthat3D-DIC is valid on monitoring the resin infusion stage and it is possible to use 2D-DIC to estimate the distribution of the fiber volume fraction on a FRP plate.

Keywords: Digital image correlation, VARTM, FRP, fiber volume fraction.

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9 SDS-induced Serine Protease Activity of an Antiviral Red Fluorescent Protein

Authors: Kalyankumar M. Matti, Chandrashekhar J. Savanurmath, Shivayogeppa B. Hinchigeri

Abstract:

A rare phenomenon of SDS-induced activation of a latent protease activity associated with the purified silkworm excretory red fluorescent protein (SE-RFP) was noticed. SE-RFP aliquots incubated with SDS for different time intervals indicated that the protein undergoes an obligatory breakdown into a number of subunits which exhibit autoproteolytic (acting upon themselves) and/or heteroproteolytic (acting on other proteins) activities. A strong serine protease activity of SE-RFP subunits on Bombyx mori nucleopolyhedrovirus (BmNPV) polyhedral protein was detected by zymography technique. A complete inhibition of BmNPV infection to silkworms was observed by the oral administration assay of the SE-RFP. Here, it is proposed that the SE-RFP prevents the initial infection of BmNPV to silkworms by obliterating the polyhedral protein. This is the first report on a silkworm red fluorescent protein that exhibits a protease activity on exposure to SDS. The present studies would help in understanding the antiviral mechanism of silkworm red fluorescent proteins.

Keywords: BmNPV, polyhedra, SE-RFP, SDS-induced protease activity, zymography.

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8 Development of a Real-Time Brain-Computer Interface for Interactive Robot Therapy: An Exploration of EEG and EMG Features during Hypnosis

Authors: Maryam Alimardani, Kazuo Hiraki

Abstract:

This study presents a framework for development of a new generation of therapy robots that can interact with users by monitoring their physiological and mental states. Here, we focused on one of the controversial methods of therapy, hypnotherapy. Hypnosis has shown to be useful in treatment of many clinical conditions. But, even for healthy people, it can be used as an effective technique for relaxation or enhancement of memory and concentration. Our aim is to develop a robot that collects information about user’s mental and physical states using electroencephalogram (EEG) and electromyography (EMG) signals and performs costeffective hypnosis at the comfort of user’s house. The presented framework consists of three main steps: (1) Find the EEG-correlates of mind state before, during, and after hypnosis and establish a cognitive model for state changes, (2) Develop a system that can track the changes in EEG and EMG activities in real time and determines if the user is ready for suggestion, and (3) Implement our system in a humanoid robot that will talk and conduct hypnosis on users based on their mental states. This paper presents a pilot study in regard to the first stage, detection of EEG and EMG features during hypnosis.

Keywords: Hypnosis, EEG, robotherapy, brain-computer interface.

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7 Variable Input Range Continuous-time Switched Current Delta-sigma Analog Digital Converter for RFID CMOS Biosensor Applications

Authors: Boram Kim, Shigeyasu Uno, Kazuo Nakazato

Abstract:

Continuous-time delta-sigma analog digital converter (ADC) for radio frequency identification (RFID) complementary metal oxide semiconductor (CMOS) biosensor has been reported. This delta-sigma ADC is suitable for digital conversion of biosensor signal because of small process variation, and variable input range. As the input range of continuous-time switched current delta-sigma ADC (Dynamic range : 50 dB) can be limited by using current reference, amplification of biosensor signal is unnecessary. The input range is switched to wide input range mode or narrow input range mode by command of current reference. When the narrow input range mode, the input range becomes ± 0.8 V. The measured power consumption is 5 mW and chip area is 0.31 mm^2 using 1.2 um standard CMOS process. Additionally, automatic input range detecting system is proposed because of RFID biosensor applications.

Keywords: continuous time, delta sigma, A/D converter, RFID, biosensor, CMOS

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6 Effect of Carbon Nanotube Reinforcement in Polymer Composite Plates under Static Loading

Authors: S. Madhu, V. V. Subba Rao

Abstract:

In the implementation of Carbon Nanotube Reinforced Polymer matrix Composites in structural applications, deflection and stress analysis are important considerations. In the present study, a multi scale analysis of deflection and stress analysis of carbon nanotube (CNT) reinforced polymer composite plates is presented. A micromechanics model based on the Mori-Tanaka method is developed by introducing straight CNTs aligned in one direction. The effect of volume fraction and diameter of CNTs on plate deflection and the stresses are investigated using classical laminate plate theory (CLPT). The study is primarily conducted with the intention of observing the suitability of CNT reinforced polymer composite plates under static loading for structural applications.

Keywords: Carbon Nanotube, Micromechanics, Composite plate, Multi-scale analysis, Classical Laminate Plate Theory.

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5 Measuring Banks’ Antifragility via Fuzzy Logic

Authors: Danielle Sandler dos Passos, Helder Coelho, Flávia Mori Sarti

Abstract:

Analysing the world banking sector, we realize that traditional risk measurement methodologies no longer reflect the actual scenario with uncertainty and leave out events that can change the dynamics of markets. Considering this, regulators and financial institutions began to search more realistic models. The aim is to include external influences and interdependencies between agents, to describe and measure the operationalization of these complex systems and their risks in a more coherent and credible way. Within this context, X-Events are more frequent than assumed and, with uncertainties and constant changes, the concept of antifragility starts to gain great prominence in comparison to others methodologies of risk management. It is very useful to analyse whether a system succumbs (fragile), resists (robust) or gets benefits (antifragile) from disorder and stress. Thus, this work proposes the creation of the Banking Antifragility Index (BAI), which is based on the calculation of a triangular fuzzy number – to "quantify" qualitative criteria linked to antifragility.

Keywords: Complex adaptive systems, X-events, risk management, antifragility, banking antifragility index, triangular fuzzy number.

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4 Forecasting the Influences of Information and Communication Technology on the Structural Changes of Japanese Industrial Sectors: A Study Using Statistical Analysis

Authors: Ubaidillah Zuhdi, Shunsuke Mori, Kazuhisa Kamegai

Abstract:

The purpose of this study is to forecast the influences of information and communication technology (ICT) on the structural changes of Japanese economies. In this study, input-output (IO) and statistical approaches are used as analysis instruments. More specifically, this study employs Leontief IO coefficients and constrained multivariate regression (CMR) model in order to achieve the purpose. The periods of initial and forecast in this study are 2005 and 2015, respectively. In this study, ICT is represented by ICT capital stocks. This study conducts two levels of analysis, namely macro and micro. The results of macro level analysis show that the dynamics of Japanese economies on the forecast period, relative to the initial period, are not so high. We focus on (1) commerce, (2) business services and office supplies, and (3) personal services sectors when conducting the analysis of the micro level. Further, we analyze its specific IO coefficients when doing this analysis. The results of the analysis explain that ICT gives a strong influence on the changes of these coefficients from initial to forecast periods.

Keywords: Forecast, ICT, Structural changes, Japanese economies.

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3 A Noble Flow Rate Control based on Leaky Bucket Method for Multi-Media OBS Networks

Authors: Kentaro Miyoko, Yoshihiko Mori, Yugo Ikeda, Yoshihiro Nishino, Yong-Bok Choi, Hiromi Okada

Abstract:

Optical burst switching (OBS) has been proposed to realize the next generation Internet based on the wavelength division multiplexing (WDM) network technologies. In the OBS, the burst contention is one of the major problems. The deflection routing has been designed for resolving the problem. However, the deflection routing becomes difficult to prevent from the burst contentions as the network load becomes high. In this paper, we introduce a flow rate control methods to reduce burst contentions. We propose new flow rate control methods based on the leaky bucket algorithm and deflection routing, i.e. separate leaky bucket deflection method, and dynamic leaky bucket deflection method. In proposed methods, edge nodes which generate data bursts carry out the flow rate control protocols. In order to verify the effectiveness of the flow rate control in OBS networks, we show that the proposed methods improve the network utilization and reduce the burst loss probability through computer simulations.

Keywords: Optical burst switching, OBS, flow rate control.

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2 Producing Outdoor Design Conditions Based on the Dependency between Meteorological Elements: Copula Approach

Authors: Zhichao Jiao, Craig Farnham, Jihui Yuan, Kazuo Emura

Abstract:

It is common to use the outdoor design weather data to select the air-conditioning capacity in the building design stage. The meteorological elements of outdoor design weather data are usually selected based on their excess frequency separately while the dependency between the elements is not well considered. It means that the simultaneous occurrence probability of these elements is smaller than the original excess frequency which may cause an overestimation of selecting air-conditioning capacity. Therefore, the copula approach which can capture the dependency between multivariate data was used to model the joint distributions of the meteorological elements, like air temperature and global solar radiation. We suggest a method based on the specific simultaneous occurrence probability of these two elements of selecting more credible outdoor design conditions. The hourly weather data at 12 noon from 2001 to 2010 in Tokyo, Japan are used to analyze the dependency structure and joint distribution, the Gaussian copula represents the dependence of data best. According to calculating the air temperature and global solar radiation in specific simultaneous occurrence probability and the common exceeding, the results show that both the air temperature and global solar radiation based on simultaneous occurrence probability are lower than these based on the conventional method in the same probability.

Keywords: Copula approach, Design weather database, energy conservation, HVAC.

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1 Utilization of 3-N-trimethylamino-1-propanol by Rhodococcus sp. strain A4 isolated from Natural Soil

Authors: Isam A. Mohamed Ahmed, Jiro Arima, Tsuyoshi Ichiyanagi, Emi Sakuno, Nobuhiro Mori

Abstract:

The aim of this study was to screen for microorganism that able to utilize 3-N-trimethylamino-1-propanol (homocholine) as a sole source of carbon and nitrogen. The aerobic degradation of homocholine has been found by a gram-positive Rhodococcus sp. bacterium isolated from soil. The isolate was identified as Rhodococcus sp. strain A4 based on the phenotypic features, physiologic and biochemical characteristics, and phylogenetic analysis. The cells of the isolated strain grown on both basal-TMAP and nutrient agar medium displayed elementary branching mycelia fragmented into irregular rod and coccoid elements. Comparative 16S rDNA sequencing studies indicated that the strain A4 falls into the Rhodococcus erythropolis subclade and forms a monophyletic group with the type-strains of R. opacus, and R. wratislaviensis. Metabolites analysis by capillary electrophoresis, fast atom bombardment-mass spectrometry, and gas chromatography- mass spectrometry, showed trimethylamine (TMA) as the major metabolite beside β-alanine betaine and trimethylaminopropionaldehyde. Therefore, the possible degradation pathway of trimethylamino propanol in the isolated strain is through consequence oxidation of alcohol group (-OH) to aldehyde (-CHO) and acid (-COOH), and thereafter the cleavage of β-alanine betaine C-N bonds yielded trimethylamine and alkyl chain.

Keywords: Homocholine, 3-N-trimethylamino-1-propanol, Quaternary ammonium compounds, 16S rDNA gene sequence.

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