@article{(Open Science Index):https://publications.waset.org/pdf/11044,
	  title     = {SDS-induced Serine Protease Activity of an Antiviral Red Fluorescent Protein},
	  author    = {Kalyankumar M. Matti and  Chandrashekhar J. Savanurmath and  Shivayogeppa B. Hinchigeri},
	  country	= {},
	  institution	= {},
	  abstract     = {A rare phenomenon of SDS-induced activation of a latent protease activity associated with the purified silkworm excretory red fluorescent protein (SE-RFP) was noticed. SE-RFP aliquots incubated with SDS for different time intervals indicated that the protein undergoes an obligatory breakdown into a number of subunits which exhibit autoproteolytic (acting upon themselves) and/or heteroproteolytic (acting on other proteins) activities. A strong serine protease activity of SE-RFP subunits on Bombyx mori nucleopolyhedrovirus (BmNPV) polyhedral protein was detected by zymography technique. A complete inhibition of BmNPV infection to silkworms was observed by the oral administration assay of the SE-RFP. Here, it is proposed that the SE-RFP prevents the initial infection of BmNPV to silkworms by obliterating the polyhedral protein. This is the first report on a silkworm red fluorescent protein that exhibits a protease activity on exposure to SDS. The present studies would help in understanding the antiviral mechanism of silkworm red fluorescent proteins.
},
	    journal   = {International Journal of Biotechnology and Bioengineering},
	  volume    = {4},
	  number    = {8},
	  year      = {2010},
	  pages     = {565 - 570},
	  ee        = {https://publications.waset.org/pdf/11044},
	  url   	= {https://publications.waset.org/vol/44},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 44, 2010},
	}