Search results for: Ahmed Shaker
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 549

Search results for: Ahmed Shaker

549 Error Propagation of the Hidden-Point Bar Method: Effect of Bar Geometry

Authors: Said M. Easa, Ahmed Shaker

Abstract:

The hidden-point bar method is useful in many surveying applications. The method involves determining the coordinates of a hidden point as a function of horizontal and vertical angles measured to three fixed points on the bar. Using these measurements, the procedure involves calculating the slant angles, the distances from the station to the fixed points, the coordinates of the fixed points, and then the coordinates of the hidden point. The propagation of the measurement errors in this complex process has not been fully investigated in the literature. This paper evaluates the effect of the bar geometry on the position accuracy of the hidden point which depends on the measurement errors of the horizontal and vertical angles. The results are used to establish some guidelines regarding the inclination angle of the bar and the location of the observed points that provide the best accuracy.

Keywords: Hidden point, accuracy, error propagation, surveying, evaluation, simulation, geometry.

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548 Characterization of the Near-Wake of an Ahmed Body Profile

Authors: Stéphanie Pellerin, Bérengére Podvin, Luc Pastur

Abstract:

In aerovehicles context, the flow around an Ahmed body profile is simulated using the velocity-vorticity formulation of the Navier-Stokes equations, associated to a penalization method for solids and Large Eddy Simulation for turbulence. The study focuses both on the ground influence on the flow and on the dissymetry of the wake, observed for a ground clearance greater than 10% of the body height H. Unsteady and mean flows are presented and analyzed. POD study completes the analysis and gives information on the most energetic structures of the flow.

Keywords: Ahmed body, bi-stability, LES, near wake.

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547 Mercury Removal Using Pseudomonas putida (ATTC 49128): Effect of Acclimatization Time, Speed and Temperature of Incubator Shaker

Authors: A. A. M. Azoddein, R. M. Yunus, N. M. Sulaiman, A. B. Bustary, K. Sabar

Abstract:

Microbes have been used to solve environmental problems for many years. The role of microorganism to sequester, precipitate or alter the oxidation state of various heavy metals has been extensively studied. Treatment using microorganism interacts with toxic metal are very diverse. The purpose of this research is to remove the mercury using Pseudomonas putida (P. putida), pure culture ATTC 49128 at optimum growth parameters such as techniques of culture, acclimatization time and speed of incubator shaker. Thus, in this study, the optimum growth parameters of P. putida were obtained to achieve the maximum of mercury removal. Based on the optimum parameters of P. putida for specific growth rate, the removal of two different mercury concentration, 1 ppm and 4 ppm were studied. From mercury nitrate solution, a mercuryresistant bacterial strain which is able to reduce from ionic mercury to metallic mercury was used to reduce ionic mercury. The overall levels of mercury removal in this study were between 80% and 89%. The information obtained in this study is of fundamental for understanding of the survival of P. putida ATTC 49128 in mercury solution. Thus, microbial mercury removal is a potential bioremediation for wastewater especially in petrochemical industries in Malaysia.

Keywords: Pseudomonas putida, growth kinetic, biosorption, mercury, petrochemical wastewater.

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546 Website Evaluation of Travel Agencies Class A in Saudi Arabia and Egypt Using Extended Version of Internet Commerce Adoption Model: A Comparative Study

Authors: Tarek Abdel Azim Ahmed, Eman Sarhan Shaker

Abstract:

This research aims to explore how well the extended model of internet commerce adoption (eMICA) model is often used to determine the extent of internet commerce adoption in the travel agencies sector in both Egypt and Kingdom of Saudi Arabia (KSA). The web content analysis method was used to analyze the level of adoption of Egyptian travel agencies and Saudi travel agencies according to data immensely available on their websites. Therefore, each site was categorized according to the phases and levels proposed. In order to achieve this, 120 websites were evaluated by the two authors over a three-month period, from August to October 2020, and then categorized according to the phases and levels of (eMICA). The results show that there are deficiencies in the application of the eMICA model by both KSA and Egyptian travel agencies, generally, updating their websites, the absence of quality certification, offering secure online payment, virtual tours, and videos using Flash animation. In general, the Egyptian companies slightly outperformed the KSA ones in applying eMICA model.

Keywords: e-commerce, eMICA, Internet marketing, travel agencies, websites.

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545 Investigation of Inter Feeder Power Flow Regulator: Load Sharing Mode

Authors: Ahmed Hossam-Eldin, Ahmed Elserougi, Ahmed Massoud, Shehab Ahmed

Abstract:

The Inter feeder Power Flow Regulator (IFPFR) proposed in this paper consists of several voltage source inverters with common dc bus; each inverter is connected in series with one of different independent distribution feeders in the power system. This paper is concerned with how to transfer power between the feeders for load sharing purpose. The power controller of each inverter injects the power (for sending feeder) or absorbs the power (for receiving feeder) via injecting suitable voltage; this voltage injection is simulated by voltage drop across series virtual impedance, the impedance value is selected to achieve the concept of power exchange between the feeders without perturbing the load voltage magnitude of each feeder. In this paper a new control scheme for load sharing using IFPFR is proposed.

Keywords: IFPFR, Load sharing, Power transfer

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544 Measurement Fractional Order Sallen-Key Filters

Authors: Ahmed Soltan, Ahmed G. Radwan, Ahmed M. Soliman

Abstract:

This work aims to generalize the integer order Sallen-Key filters into the fractional-order domain. The analysis in the case of two different fractional-order elements introduced where the general transfer function becomes four terms which is unusual in the conventional case. In addition, the effect of the transfer function parameters on the filter poles and hence the stability is introduced and closed forms for the filter critical frequencies are driven. Finally, different examples for the fractional order Sallen-Key filter design are presented with circuit simulations using ADS where a great matching between the numerical and simulation results is obtained.

Keywords: Analog Filter, Low-Pass Filter, Fractance, Sallen-Key, Stability.

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543 Comparative Evaluation of Ice Adhesion Behavior

Authors: T. Strobl, D. Raps, M. Hornung

Abstract:

In this study, the adhesion of ice to solid substrates with different surface properties is compared. Clear ice, similar to atmospheric in-flight icing encounters, is accreted on the different substrates under controlled conditions. The ice adhesion behavior is investigated by means of a dynamic vibration testing technique with an electromagnetic shaker initiating ice de-bonding in the interface between the substrate and the ice. The results of the experiments reveal that the affinity for ice accretion is significantly influenced by the water contact angle of the respective sample.

Keywords: Contact angle, dynamic vibration measurement, ice adhesion, interfacial shear stress.

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542 The Clinical Use of Ahmed Valve Implant as an Aqueous Shunt for Control of Uveitic Glaucoma in Dogs

Authors: Khaled M. Ali, M. A. Abdel-Hamid, Ayman A. Mostafa

Abstract:

Objective: Safety and efficacy of Ahmed glaucoma valve implantation for the management of uveitis induced glaucoma evaluated on the five dogs with uncontrollable glaucoma. Materials and Methods: Ahmed Glaucoma Valve (AGV®; New World Medical, Rancho Cucamonga, CA, USA) is a flow restrictive, nonobstructive self-regulating valve system. Preoperative ocular evaluation included direct ophthalmoscopy and measurement of the intraocular pressure (IOP). The implant was examined and primed prior to implantation. The selected site of the valve implantation was the superior quadrant between the superior and lateral rectus muscles. A fornix-based incision was made through the conjunectiva and Tenon’s capsule. A pocket is formed by blunt dissection of Tenon’s capsule from the episclera. The body of the implant was inserted into the pocket with the leading edge of the device around 8-10 mm from the limbus. Results: No post-operative complications were detected in the operated eyes except a persistent corneal edema occupied the upper half of the cornea in one case. Hyphaema was very mild and seen only in two cases which resolved quickly two days after surgery. Endoscopical evaluation for the operated eyes revealed a normal ocular fundus with clearly visible optic papilla, tapetum and retinal blood vessels. No evidence of hemorrhage, infection, adhesions or retinal abnormalities was detected. Conclusion: Ahmed glaucoma valve is safe and effective implant for treatment of uveitic glaucoma in dogs.

Keywords: Ahmed valve, endoscopy, glaucoma, ocular fundus.

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541 EEG Waves Classifier using Wavelet Transform and Fourier Transform

Authors: Maan M. Shaker

Abstract:

The electroencephalograph (EEG) signal is one of the most widely signal used in the bioinformatics field due to its rich information about human tasks. In this work EEG waves classification is achieved using the Discrete Wavelet Transform DWT with Fast Fourier Transform (FFT) by adopting the normalized EEG data. The DWT is used as a classifier of the EEG wave's frequencies, while FFT is implemented to visualize the EEG waves in multi-resolution of DWT. Several real EEG data sets (real EEG data for both normal and abnormal persons) have been tested and the results improve the validity of the proposed technique.

Keywords: Bioinformatics, DWT, EEG waves, FFT.

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540 Drilling of Glass Sheets by Abrasive Jet Machining

Authors: A. El-Domiaty, H. M. Abd El-Hafez, M. A. Shaker

Abstract:

Drilling of glass sheets with different thicknesses have been carried out by Abrasive Jet Machining process (AJM) in order to determine its machinability under different controlling parameters of the AJM process. The present study has been introduced a mathematical model and the obtained results have been compared with that obtained from other models published earlier [1-6]. The experimental results of the present work are used to discuss the validity of the proposed model as well as the other models.

Keywords: Abrasive Jet Machining, Erosion rate, Glass, Mathematical model.

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539 EEG Spikes Detection, Sorting, and Localization

Authors: Mazin Z. Othman, Maan M. Shaker, Mohammed F. Abdullah

Abstract:

This study introduces a new method for detecting, sorting, and localizing spikes from multiunit EEG recordings. The method combines the wavelet transform, which localizes distinctive spike features, with Super-Paramagnetic Clustering (SPC) algorithm, which allows automatic classification of the data without assumptions such as low variance or Gaussian distributions. Moreover, the method is capable of setting amplitude thresholds for spike detection. The method makes use of several real EEG data sets, and accordingly the spikes are detected, clustered and their times were detected.

Keywords: EEG time localizations, EEG spike detection, superparamagnetic algorithm, wavelet transform.

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538 Response Spectrum Transformation for Seismic Qualification Testing

Authors: Nouredine Bourahla, Farid Bouriche, Yacine Benghalia

Abstract:

Seismic qualification testing for equipments to be mounted on upper storeys of buildings is very demanding in terms of floor spectra. The latter is characterized by high accelerations amplitudes within a narrow frequency band. This article presents a method which permits to cover specified required response spectra beyond the shaking table capability by amplifying the acceleration amplitudes at an appropriate frequency range using a physical intermediate mounted on the platform of the shaker.

Keywords: floor spectra, response spectrum, seismicqualification testing, shaking table

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537 A New Algorithm for Solving Isothermal Carbonization of Wood Particle

Authors: Ahmed Mahmoudi, Imen Mejri, Mohamed A. Abbassi, Ahmed Omri

Abstract:

A new algorithm based on the lattice Boltzmann method (LBM) is proposed as a potential solver for one-dimensional heat and mass transfer for isothermal carbonization of wood particles. To check the validity of this algorithm, the LBM results have been compared with the published data and a good agreement is obtained. Then, the model is used to study the effect of reactor temperature and particle size on the evolution of the local temperature and mass loss inside the wood particle.

Keywords: Lattice Boltzmann Method, pyrolysis, conduction, carbonization.

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536 Removal of Methylene Blue from Aqueous Solution by Using Gypsum as a Low Cost Adsorbent

Authors: Muhammad A.Rauf, I.Shehadeh, Amal Ahmed, Ahmed Al-Zamly

Abstract:

Removal of Methylene Blue (MB) from aqueous solution by adsorbing it on Gypsum was investigated by batch method. The studies were conducted at 25°C and included the effects of pH and initial concentration of Methylene Blue. The adsorption data was analyzed by using the Langmuir, Freundlich and Tempkin isotherm models. The maximum monolayer adsorption capacity was found to be 36 mg of the dye per gram of gypsum. The data were also analyzed in terms of their kinetic behavior and was found to obey the pseudo second order equation.

Keywords: Adsorption, Dye, Gypsum, Kinetics, Methylene Blue.

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535 Analysis of Conduction-Radiation Heat Transfer in a Planar Medium: Application of the Lattice Boltzmann Method

Authors: Ahmed Mahmoudi, Imen Mejri, Mohamed A. Abbassi, Ahmed Omri

Abstract:

In this paper, the 1-D conduction-radiation problem is solved by the lattice Boltzmann method. The effects of various parameters such as the scattering albedo, the conduction–radiation parameter and the wall emissivity are studied. In order to check on the accuracy of the numerical technique employed for the solution of the considered problem, the present numerical code was validated with the published study. The found results are in good agreement with those published

Keywords: Conduction, lattice Boltzmann method, planar medium, radiation.

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534 A Physically-Based Analytical Model for Reduced Surface Field Laterally Double Diffused MOSFETs

Authors: M. Abouelatta, A. Shaker, M. El-Banna, G. T. Sayah, C. Gontrand, A. Zekry

Abstract:

In this paper, a methodology for physically modeling the intrinsic MOS part and the drift region of the n-channel Laterally Double-diffused MOSFET (LDMOS) is presented. The basic physical effects like velocity saturation, mobility reduction, and nonuniform impurity concentration in the channel are taken into consideration. The analytical model is implemented using MATLAB. A comparison of the simulations from technology computer aided design (TCAD) and that from the proposed analytical model, at room temperature, shows a satisfactory accuracy which is less than 5% for the whole voltage domain.

Keywords: LDMOS, MATLAB, RESURF, modeling, TCAD.

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533 Modeling and Analysis of SVPWM Based Dynamic Voltage Restorer

Authors: Ahmed Helal, Sherif Zain Elabideen, Ahmed Lotfy

Abstract:

In this paper the modeling and analysis of Space Vector Pulse Width Modulation (SVPWM) based Dynamic Voltage Restorer (DVR) using PSCAD/EMTDC software will be presented in details. The simulation includes full modeling of the SVPWM technique used to control the DVR inverter. A test power system composed of three phase voltage source, sag generator, DVR and three phase resistive load is used to demonstrate restoration capability of the DVR. The simulation results of the presented DVR proved excellent voltage sag mitigation to protect sensitive loads.

Keywords: Dynamic voltage restorer, power quality, simulationand modeling, voltage sag.

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532 Lattice Boltzmann Simulation of the Carbonization of Wood Particle

Authors: Ahmed Mahmoudi, Imen Mejri, Mohamed A. Abbassi, Ahmed Omri

Abstract:

A numerical study based on the Lattice Boltzmann Method (LBM) is proposed to solve one, two and three dimensional heat and mass transfer for isothermal carbonization of thick wood particles. To check the validity of the proposed model, computational results have been compared with the published data and a good agreement is obtained. Then, the model is used to study the effect of reactor temperature and thermal boundary conditions, on the evolution of the local temperature and the mass distributions of the wood particle during carbonization

Keywords: Lattice Boltzmann Method, pyrolysis conduction, carbonization, Heat and mass transfer.

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531 Radiation Heat Transfer Effect in Solid Oxide Fuel Cell: Application of the Lattice Boltzmann Method

Authors: Imen Mejri, Ahmed Mahmoudi, Mohamed A. Abbassi, Ahmed Omri

Abstract:

The radiation effect within the solid anode, electrolyte, and cathode SOFC layers problem has been investigated in this paper. Energy equation is solved by the Lattice Boltzmann method (LBM). The Rosseland method is used to model the radiative transfer in the electrodes. The Schuster-Schwarzschild method is used to model the radiative transfer in the electrolyte. Without radiative effect, the found results are in good agreement with those published. The obtained results show that the radiative effect can be neglected.

Keywords: SOFC, lattice Boltzmann method, conduction, radiation.

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530 Performance Analysis of Selective Adaptive Multiple Access Interference Cancellation for Multicarrier DS-CDMA Systems

Authors: Maged Ahmed, Ahmed El-Mahdy

Abstract:

In this paper, Selective Adaptive Parallel Interference Cancellation (SA-PIC) technique is presented for Multicarrier Direct Sequence Code Division Multiple Access (MC DS-CDMA) scheme. The motivation of using SA-PIC is that it gives high performance and at the same time, reduces the computational complexity required to perform interference cancellation. An upper bound expression of the bit error rate (BER) for the SA-PIC under Rayleigh fading channel condition is derived. Moreover, the implementation complexities for SA-PIC and Adaptive Parallel Interference Cancellation (APIC) are discussed and compared. The performance of SA-PIC is investigated analytically and validated via computer simulations.

Keywords: Adaptive interference cancellation, communicationsystems, multicarrier signal processing, spread spectrum

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529 A Systematic Approach for Design a Low-Cost Mobility Assistive Device for Elderly People

Authors: Omar Salah, Ahmed A. Ramadan, Salvatore Sessa, Ahmed A. Abo-Ismail

Abstract:

Walking and sit to stand are activities carried out by all the people many times during the day, but physical disabilities due to age and diseases create needs of assistive devices to help elderly people during their daily life. This study aims to study the different types and mechanisms of the assistive devices. We will analyze the limitations and the challenges faced by the researchers in this field. We will introduce the Assistive Device developed at the Egypt-Japan University of Science and Technology, named E-JUST Assistive Device (EJAD). EJAD will be a low cost intelligent assistive device to help elders in walking and sit-to-stand activities.

Keywords: Active walker, Assistive robotics, Standing Assistance, Walking Assistance

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528 Kinetics Study of Ammonia Removal from Synthetic Waste Water

Authors: Edison Muzenda, John Kabuba, Freeman Ntuli, Mansoor Mollagee

Abstract:

The aim of this study was to investigate ammonium exchange capacity of natural and activated clinoptilolite from Kwazulu-Natal Province, South Africa. X – ray fluorescence (XRF) analysis showed that the clinoptilolite contained exchangeable ions of sodium, potassium, calcium and magnesium. This analysis also confirmed that the zeolite sample had a high silicon composition compared to aluminium. Batch equilibrium studies were performed in an orbital shaker and the data fitted the Langmuir isotherm very well. The ammonium exchange capacity was found to increase with pH and temperature. Clinoptilolite functionalization with hydrochloric acid increased its ammonia uptake ability.

Keywords: Activated clinoptilolite, Ammonium exchange, Equilibrium, Functionalization, Langmuir isotherm

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527 Effects of Asphalt Modification with Nanomaterials on Fresh and Stored Bitumen

Authors: Ahmed W. Oda, Ahmed El-Desouky, Hassan Mahdy, Osama M. Moussa

Abstract:

Nanomaterials have many applications in the field of asphalt paving. Two locally produced nanomaterials were used in the asphalt binder modification. The nanomaterials used are Nanosilica (NS), and Nanoclay (NC). The virgin asphalt binder was characterized by the conventional tests. The bitumen was modified by 3%, 5% and 7% of NS and NC. The penetration index (PI), and the retaining penetration (RP) was calculated based on the results of the penetration and the softening point tests. The results show that the RP becomes 95.35% at 5% NS modified bitumen and reaches 97.56% when bitumen is modified with 3% NC. The results show significant improvement in the bitumen stiffness when modified by the two types of nanomaterials, either fresh or aged (stored).

Keywords: Bitumen, modified bitumen, aged, stored, nanomaterials.

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526 Steady State Temperature Distribution of Cast-Resin Dry Type Transformer Based on New Thermal Model Using Finite Element Method

Authors: Magdy B. Eteiba, Essam A. Alzahab, Yomna O. Shaker

Abstract:

In this paper, a thermal model of cast- resin dry type transformer is proposed. The proposed thermal model is solved by finite element technique to get the temperature at any location of the transformer. The basic modes of heat transfer such as conduction; convection and radiation are used to get the steady state temperature distribution of the transformer. The predicted temperatures are compared with experimental results reported in this paper and it is found a good agreement between them. The effects of various parameters such as width of air duct, ambient temperature and emissivity of the outer surface were also studied.

Keywords: Convection, dry type transformer, finite-elementtechnique, thermal model.

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525 Coproduction of Fructose and Ethanol from Dates by S. cerevisiae ATCC 36859

Authors: M. A. Zeinelabdeen, A. E. Abasaeed, M. H. Gaily, A. K. Sulieman, M. D. Putra

Abstract:

Coproduction of fructose and ethanol from dates extract by a glucose-selective S. cerevisiae ATCC 36859 strain has been studied. Various initial sugar concentrations (i.e., 131.4, 315.3, 408.2, and 500.0 g/l) have been tested. The fermentation experiments were performed in a water shaker bath at 30°C and 120 rpm. The results showed that highest yields of fructose (95.0%) and ethanol (72.8%) were achieved for the 131.4 g/l concentration. Increasing the initial concentration to 315.3 g/l resulted in lower yields of fructose (82.2%) and ethanol (61.0%). However, further increase to 408.2 g/l increased the fructose yield (97.5%) at the expense of ethanol yield (42.0%) due to probable substrate inhibitions that resulted in lower glucose conversion. At 500 g initial sugar/l the growth rate of ATCC 36859 was highly inhibited. 

Keywords: Dates, ethanol, fructose, fermentation, S. cerevisiae.

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524 Optimal Design of Substation Grounding Grid Based on Genetic Algorithm Technique

Authors: Ahmed Z. Gabr, Ahmed A. Helal, Hussein E. Said

Abstract:

With the incessant increase of power systems capacity and voltage grade, the safety of grounding grid becomes more and more prominent. In this paper, the designing substation grounding grid is presented by means of genetic algorithm (GA). This approach purposes to control the grounding cost of the power system with the aid of controlling grounding rod number and conductor lengths under the same safety limitations. The proposed technique is used for the design of the substation grounding grid in Khalda Petroleum Company “El-Qasr” power plant and the design was simulated by using CYMGRD software for results verification. The result of the design is highly complying with IEEE 80-2000 standard requirements.

Keywords: Genetic algorithm, optimum grounding grid design, power system analysis, power system protection, single layer model, substation.

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523 A New Floating Point Implementation of Base 2 Logarithm

Authors: Ahmed M. Mansour, Ali M. El-Sawy, Ahmed T Sayed

Abstract:

Logarithms reduce products to sums and powers to products; they play an important role in signal processing, communication and information theory. They are primarily used for hardware calculations, handling multiplications, divisions, powers, and roots effectively. There are three commonly used bases for logarithms; the logarithm with base-10 is called the common logarithm, the natural logarithm with base-e and the binary logarithm with base-2. This paper demonstrates different methods of calculation for log2 showing the complexity of each and finds out the most accurate and efficient besides giving insights to their hardware design. We present a new method called Floor Shift for fast calculation of log2, and then we combine this algorithm with Taylor series to improve the accuracy of the output, we illustrate that by using two examples. We finally compare the algorithms and conclude with our remarks.

Keywords: Logarithms, log2, floor, iterative, CORDIC, Taylor series.

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522 Power Frequency Magnetic Field Survey in Indoor Power Distribution Substation in Egypt

Authors: Ahmed Hossam_ ElDin, Ahmed Farag, Ibrahim Madi., Hanaa Karawia

Abstract:

In our modern society electricity is vital to our health, safety, comfort and well-being. While our daily use of electricity is often taken for granted, public concern has arisen about potential adverse health effects from electric and magnetic – electromagnetic – fields (EMFs) produced by our use of electricity. This paper aims to compare between the measured magnetic field values and the simulated models for the indoor medium to low voltage (MV/LV) distribution substations. To calculate the magnetic flux density in the substations, interactive software SUBCALC is used which is based on closed form solution of the Biot-Savart law with 3D conductor model. The comparison between the measured values and the simulated models was acceptable. However there were some discrepancies, as expected, may be due to the current variation during measurements.

Keywords: Distribution substation, magnetic field, measurement, simulation.

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521 Analysis and Simulation of Automotive Interleaved Buck Converter

Authors: Mohamed. A. Shrud, Ahmad H. Kharaz, Ahmed. S. Ashur, Ahmed Faris, Mustafa Benamar

Abstract:

This paper will focus on modeling, analysis and simulation of a 42V/14V dc/dc converter based architecture. This architecture is considered to be technically a viable solution for automotive dual-voltage power system for passenger car in the near further. An interleaved dc/dc converter system is chosen for the automotive converter topology due to its advantages regarding filter reduction, dynamic response, and power management. Presented herein, is a model based on one kilowatt interleaved six-phase buck converter designed to operate in a Discontinuous Conduction Mode (DCM). The control strategy of the converter is based on a voltagemode- controlled Pulse Width Modulation (PWM) with a Proportional-Integral-Derivative (PID). The effectiveness of the interleaved step-down converter is verified through simulation results using control-oriented simulator, MatLab/Simulink.

Keywords: Automotive, dc-to-dc power modules, design, interleaved, Matlab\Simulink and PID control.

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520 Glutamic Acid Production from Potato by Brevibacterium linens

Authors: Marzieh Moosavi-Nasab, Masoumeh Izadi, Sara Hosseinpour

Abstract:

In this study, the possibility of using potato as a substrate for glutamic acid production by Brevibacterium linens was investigated. For preparation of fermentation medium, potato was hydrolyzed by hydrochloridric acid. The medium contained potato hydrolysate, tween 80, mineral solution, glucose, and potassium hydrogen phosphate. The initial pH of the medium was adjusted to 7-7.5. For achieving the optimum time with maximum yield, the beakers containing the medium and the inoculums were incubated in a rotary water bath flask shaker for one to five days. Thin layer choromatography was used for quantitative and qualitative assay of the glutamic acid produced. The results revealed that as fermentation time increased, pH of the fermentation medium significantly decreased (P<0.05). Furthermore, glutamic acid concentration in fermentation medium increased significantly (P<0.05). The highest amount of the glutamic acid obtained was 5.6 g/l on the forth day of fermentation.

Keywords: Brevibacterium linens, Fermentation, Glutamicacid, Thin layer choromatography

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