Coproduction of Fructose and Ethanol from Dates by S. cerevisiae ATCC 36859
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 33093
Coproduction of Fructose and Ethanol from Dates by S. cerevisiae ATCC 36859

Authors: M. A. Zeinelabdeen, A. E. Abasaeed, M. H. Gaily, A. K. Sulieman, M. D. Putra

Abstract:

Coproduction of fructose and ethanol from dates extract by a glucose-selective S. cerevisiae ATCC 36859 strain has been studied. Various initial sugar concentrations (i.e., 131.4, 315.3, 408.2, and 500.0 g/l) have been tested. The fermentation experiments were performed in a water shaker bath at 30°C and 120 rpm. The results showed that highest yields of fructose (95.0%) and ethanol (72.8%) were achieved for the 131.4 g/l concentration. Increasing the initial concentration to 315.3 g/l resulted in lower yields of fructose (82.2%) and ethanol (61.0%). However, further increase to 408.2 g/l increased the fructose yield (97.5%) at the expense of ethanol yield (42.0%) due to probable substrate inhibitions that resulted in lower glucose conversion. At 500 g initial sugar/l the growth rate of ATCC 36859 was highly inhibited. 

Keywords: Dates, ethanol, fructose, fermentation, S. cerevisiae.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1088526

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2678

References:


[1] K. Selim, M. AbdeLe-Bary, and O. Ismaael, "Effect of irradiation and heat treatments on the quality characteristics of siwi date fruit (Phoenis dactyliferal)," AgroLife Scientific J., vol. 1, pp. 103-111, 2012.
[2] A. A. Al- Qarawi, B. H. Ali, S. A. Al-Mougy, and H. M. Mousa, "Gastrointestinal transit in mice treated with various extracts of date (Phoenix dactylifera L.)," Food Chem Toxicol., vol. 41, no. 1, pp. 37- 39, Jan. 2003.
[3] S. M. Jain, "In vitro mutagenesis for improving date palm (Phoenix dactylifera L.)," Emir. J. Food Agric., vol. 24, no. 5, pp. 400-407, 2012.
[4] F. Al-shreed, et al, "A study on the export of Saudi Arabian dates in the global markets," JDAE., vol. 4, no. 9, pp. 268-274, May. 2012.
[5] N. A. Al-Wusaibai, A. Ben Abdallah, M. S. Al-Husainai, H. Al-Salman and M. Elballaj, "A comparative study between mechanical and manual pollination in two premier Saudi Arabian date palm cultivars," INDJST., vol. 5, no. 4, pp. 2487-2490, Apr. 2012.
[6] S. Moshaf, Z. H. Esfahani, and M. H. Aziz, "Optimization of conditions for Xanthan gum production from waste date in submerged fermentation," WASET., vol. 57, pp. 521-524, 2011.
[7] M. D. Putra, et al, "Production of fructose from highly concentrated dates extract (Periodical style—Accepted for publication)," Biotech. Lett., to be published.
[8] A. Hasnaoui, et al, "Chemical composition and microbial quality of dates grown in Figuig oasis of Morocco,"IJAB., vol. 12, no.2, pp. 311- 314, Mar. 2010.
[9] A. K. Mostafazadeh, M. Sarshar, Sh. Javadian, M. R. Zarefard, and Z. A. Haghighi, "Separation of fructose and glucose from date syrup using resin chromatographic method: experimental data and mathematical modeling," Sep Purif Technol., vol. 79, no. 1, pp. 72-78, May. 2011.
[10] D. M. Lima, et al, "Fructose syrup: a biotechnology asset," Food Technol. Biotechnol., vol. 49, no. 4, pp. 424-434, Oct. 2011.
[11] S. E. Fleming, and J. W. Grootwasink, "Preparation of high-fructose syrup from the tubers of the Jerusalem artichoke (Helianthus tuberosus L.)," Crit Rev Food Sc., vol. 12, no. 1, pp. 1-28, Nov. 1979.
[12] L. M. Hanover, and J. S. White, "Manufacturing, composition, and applications of fructose1’2," AJCN., vol. 58, no. 5, pp. 724S-732S, Dec. 1993.
[13] I. M. Al-Nashef, M. H. Gaily, S. M. Al-Zahrani, and A. E. Abasaeed, "Method for separating fructose and glucose,"US 7942972 B2, May. 2011.
[14] H. Atiyeh, and Z. Duvnjak, "Study of the production of fructose and ethanol from sucrose media by Saccharomyces cerevisiae," Appl Microbiol Biotechnol., vol. 57, no. 3, pp. 407-411, Oct. 2001.
[15] D. W. Koren, and Z. Duvnjak, "Fed-batch production of concentrated fructose syrup and ethanol using Saccharomyces cerevisiae ATCC 36859," Acta Biotechnol., vol. 12, no. 6, pp. 489-496, 1992.
[16] M. H. Gaily, et al, "The effect of activation time on the production of fructose and bioethanol from date extract," AJB., vol. 11, no. 33, pp. 8212-8217, Apr . 2012.
[17] A. K. Sulieman, M. H. Gaily, M. A. Zeinelabdeen, M. D. Putra, and A. E. Abasaeed, "Production of bioethanol fuel from low-grade-date extract,"IJCEA., vol. 4, no. 3, pp. 140-143, Jun. 2013.
[18] S. Govindaswamy, and L. M. Vane, "Kinetics of growth and ethanol production on different carbon substrates using genetically engineered xylose-fermenting yeast," Bioresource Technol., vol. 98, no. 3, pp. 677- 685, Feb. 2007.
[19] M. Balat, H. Balat, and C. Oz, "Progress in bioethanol processing," Prog Energ Combust., vol. 34, no. 5, pp. 551-573, Oct. 2008.
[20] M. E. E. Abashar, and S. S. E. E. Elnashaie, "Dynamic and chaotic behavior of periodically forced fermentors for bioethanol production," Chem Eng Sci., vol. 65, no. 16, pp. 4894-4905, Aug. 2010.