Search results for: fuzzy logic control.

Authors: Abdulmajeed Bashir Mlitan, Ethan Hack

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Soil samples were collected from and around Alkomos cement factory, Alkomos town, Libya. Soil physiochemical properties were determined. In addition, olive leaves were scanned for their fungal content. This work can conclude that the results obtained for the examined physiochemical characteristics of soil in the area studied prove that cement dust from the Alkomos cement factory in Libya has had a significant impact on the soil. The affected soil properties are pH and total calcium content. These characteristics were found to be higher than those in similar soils from the same area. The increment of soil pH in the same area may be a result of precipitation of cement dust over the years. Different responses were found in each season and each site. For instance, the dominance of fungi of soil and leaves was lowest at 100 m from the factory and the evenness and diversity increased at this site compared to the control area and 250 m from the factory.

Keywords: Pollution, Soil Microbial, Alkomos, Libya.

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Authors: E. Farsad, S. P. Abbasi, A. Goodarzi, M. S. Zabihi

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Nowadays, quasi-continuous wave diode lasers are used in a widespread variety of applications. Temperature effects in these lasers can strongly influence their performance. In this paper, the effects of temperature have been experimentally investigated on different features of a 60W-QCW diode laser. The obtained results indicate that the conversion efficiency and operation voltage of diode laser decrease with the augmentation of the working temperature associated with a redshift in the laser peak wavelength. Experimental results show the emission peak wavelength of laser shifts 0.26 nm and the conversion efficiency decreases 1.76 % with the increase of temperature from 40 to 50 ̊C. Present study also shows the slope efficiency decreases gradually at low temperatures and rapidly at higher temperatures. Regarding the close dependence of the mentioned parameters to the operating temperature, it is of great importance to carefully control the working temperature of diode laser, particularly for medical applications.

Keywords: diode laser, experimentally, temperature, wavelength

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Authors: S.C. Chin, N. Shafiq, M.F. Nuruddin

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In the modern construction practices, industrial wastes or by-products are largely used as raw materials in cement and concrete. These impart many benefits to the environment and bringabout an economic impact because the cost of waste disposal is constantly increasing due to strict environmental regulations. It was reported in literature that the leakage of oil onto concrete element in older cement grinding unit resulted in concrete with greater resistance to freezing and thawing. This effect was thought to be similar to adding an air-entraining chemical admixture to concrete. This paper presents an investigation on the load deflection behaviour and crack patterns of reinforced concrete (RC) beams subjected to four point loading. Ten 120x260x1900 mm beams were cast with 100% ordinary Portland cement (OPC) concrete, 20% fly ash (FA) and 20% rice husk ash (RHA) blended cement concrete. 0.15% dosage of admixtures (used engine oil, new engine oil, and superplasticizer) was used throughout the experiment. Results show that OPC and OPC/RHA RC beams containing used engine oil and superplasticizer exhibit higher capacity, 18-26% than their corresponding control mix.

Keywords: by-products, RC beams, superplasticizer, used engine oil

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Authors: L. Labéjof, I. Mororó, P. Galle, G. Barbosa, M.I. Severo, A.H. de Oliveira

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Ultrastructure of duodenum mucosa of irradiated rat was studied versus dose rate of irradiation following exposure to gamma rays from 60-Cobalt source. The animals were whole body irradiated at two dose rates (1 Gy.mn-1 and 1 Gy.h-1) and three total doses (1, 2 or 4 Gy) for each dose rate. 24 or 48 h after irradiation, their small intestine was removed and samples of duodenum were processed for observations under a transmission electron microscopy. Samples of duodenum mucosa of control rats were processed in the same way. For the lower dose rate of 1 Gy.h-1, main lesions characteristic of apoptosis were detected within irradiated enterocytes at a total dose of 2 Gy and 24 h after exposure. Necrosis was noted in the samples, 48 h after exposition. For the higher dose rate of 1 Gy.mn-1, fewer changes were detected at all total doses 24 or 48 h irradiation. Thus, it was shown that the appearance of radiationinduced alterations varies not only with increasing total dose and post-irradiation time but especially with decreasing dose rate.

Keywords: Dose rate, Radiation Inury, Apoptosis, SmallBowel

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Authors: L. Grinis, V. Haslavsky, U. Tzadka

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This paper describes an experimental, theoretical model and numerical study of concentrated vortex flow past a sphere in a hydraulic check valve. The phenomenon of the rotation of the ball around the axis of the device through which liquid flows has been found. That is, due to the rotation of the sphere in the check valve vibration is caused. We observe the rotation of the sphere around the longitudinal axis of the check valve. This rotation is induced by a vortex shedding from the sphere. We will discuss computational simulation and experimental investigations of this strong sphere rotation. The frequency of the sphere vibration and interaction with the check valve wall has been measured as a function of the wide range Reynolds Number. The validity of the computational simulation and of the assumptions on which it is based has been proved experimentally. This study demonstrates the possibility to control the vibrations in a hydraulic system and proves to be very effective suppression of the self-excited vibration.

Keywords: Check-valve, vibration, vortex shedding

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Authors: Anant Oonsivilai, Kenedy A. Greyson

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The growth and interconnection of power networks in many regions has invited complicated techniques for energy management services (EMS). State estimation techniques become a powerful tool in power system control centers, and that more information is required to achieve the objective of EMS. For the online state estimator, assuming the continuous time is equidistantly sampled with period Δt, processing events must be finished within this period. Advantage of Kalman Filtering (KF) algorithm in using system information to improve the estimation precision is utilized. Computational power is a major issue responsible for the achievement of the objective, i.e. estimators- solution at a small sampled period. This paper presents the optimum utilization of processors in a state estimator based on KF. The model used is presented using Petri net (PN) theory.

Keywords: Kalman filters, model, Petri Net, power system, sequential State estimator.

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Authors: Salvatore L., Pires B., Campos M. C. M., De Souza Jr M. B.

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It is estimated that the total cost of abnormal conditions to US process industries is around $20 billion dollars in annual losses. The hydrotreatment (HDT) of diesel fuel in petroleum refineries is a conversion process that leads to high profitable economical returns. However, this is a difficult process to control because it is operated continuously, with high hydrogen pressures and it is also subject to disturbances in feed properties and catalyst performance. So, the automatic detection of fault and diagnosis plays an important role in this context. In this work, a hybrid approach based on neural networks together with a pos-processing classification algorithm is used to detect faults in a simulated HDT unit. Nine classes (8 faults and the normal operation) were correctly classified using the proposed approach in a maximum time of 5 minutes, based on on-line data process measurements.

Keywords: Fault detection, hydrotreatment, hybrid systems, neural networks.

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Authors: Yu-Ching Yang, Haw-Long Lee, Win-Jin Chang

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The present work numerically analyzes the transient heat transfer in the absorber plates of a flat-plate solar collector based on the dual-phase-lag (DPL) heat conduction model. An efficient numerical scheme involving the hybrid application of the Laplace transform and control volume methods is used to solve the linear hyperbolic heat conduction equation. This work also examines the effect of different medium parameters on the behavior of heat transfer. Results show that, while the heat-flux phase lag induces thermal waves in the medium, the temperature-gradient phase lag smoothens the thermal waves by promoting non-Fourier diffusion-like conduction into the medium.

Keywords: Absorber plates, dual-phase-lag, non-Fourier, solar collector.

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Authors: I. Slim, H. Akkari, A. Ben Abdallah, I. Bhouri, M. Hedi Bedoui

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Osteoporosis is a common disease characterized by low bone mass and deterioration of micro-architectural bone tissue, which provokes an increased risk of fracture. This work treats the texture characterization of trabecular bone radiographs. The aim was to analyze according to clinical research a group of 174 subjects: 87 osteoporotic patients (OP) with various bone fracture types and 87 control cases (CC). To characterize osteoporosis, Fractal and MultiFractal (MF) methods were applied to images for features (attributes) extraction. In order to improve the results, a new method of MF spectrum based on the q-stucture function calculation was proposed and a combination of Fractal and MF attributes was used. The Support Vector Machines (SVM) was applied as a classifier to distinguish between OP patients and CC subjects. The features fusion (fractal and MF) allowed a good discrimination between the two groups with an accuracy rate of 96.22%.

Keywords: Fractal, micro-architecture analysis, multifractal, SVM, osteoporosis.

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Authors: Martha C. Orazulume, Jibril D. Jiya

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Flexible satellites are equipped with various appendages which vibrate under the influence of any excitation and make the attitude of the satellite to be unstable. Therefore, the system must be able to adjust to balance the effect of these appendages in order to point accurately and satisfactorily which is one of the most important problems in satellite design. Proportional Integral Derivative (PID) Controller is simple to design and computationally efficient to implement which is used to stabilize the effect of these flexible appendages. However, manual turning of the PID is time consuming, waste energy and money. Particle Swarm Optimization (PSO) is used to tune the parameters of PID Controller. Simulation results obtained show that PSO tuned PID Controller is able to re-orient the spacecraft attitude as well as dampen the effect of mechanical resonance and yields better performance when compared with manually tuned PID Controller.

Keywords: Attitude control, flexible satellite, particle swarm optimization, PID controller.

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Authors: Alireza M. goltabar, Issa shooshpasha , Reza Shamstabar kami , Mostafa Habibi

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Added stresses due to adjacent structure should be considered in foundation design and stress control in soil under the structure. This case is considered less than other cases in design and calculation whereas stresses in implementation are greater than analytical stress. Structure load are transmitted to earth by foundation and role of foundation is propagation of load on the continuous and half extreme soil. This act cause that, present stresses lessen to allowable strength of soil. Some researchers such as Boussinesq and westergaurd by using of some assumption studied on this issue, theorically. Target of this paper is study and evaluation of added stresses under structure due to adjacent structure. For this purpose, by using of assumption, theoric relation and numeral methods, effects of adjacent structure with 4 to 10 storeys on the main structure with 4 storeys are studied and effect of parameters and sensitivity of them are evaluated.

Keywords: stress, soil, adjacent structure, foundation, loading.

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Authors: Astrid de Leeuw, Pierre Valois

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Environmental education is the key to enhancing or changing students’ ways of thinking and acting in order to create an environmentally robust future for all. The present study investigates the beliefs of 812 primary school students, which merit consideration when developing educational interventions. Results of multiple regression analyses reveal that educational interventions should focus on promoting students’ feelings of control over pro-environmental behaviors (PEB). For example, schools could provide recycling bins on the premises. Furthermore, it is critical to develop positive attitudes in students by stressing the various benefits of PEB for keeping our planet clean and protecting wildlife. Unfortunately, our results indicate that students believe that PEB is boring and annoying. Suggestions are offered for making PEB more interesting and relevant. Further research is needed to test the effectiveness of interventions based on the present results.

Keywords: Pro-environmental behaviors, primary school students, theory of planned behavior, beliefs, educational interventions.

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Authors: Hamid Rafiei, Reza Janamiri, Mohammad Hossein Sedaghat, Amir Hatampour

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In this work, the results of mixing study by a jet mixer in a tank have been investigated in the laboratory scale. The tank dimensions are H/D=1 and the jet entrance have been considered in the center of upper surface of tank. RNG-k-ε model is used as the turbulent model for the prediction of the pattern of turbulent flow inside the tank. For this purpose, a tank with volume of 110 liter is simulated and it has been divided into 410,000 tetrahedral control cells for performing the calculations. The grids at the vicinity of the nozzle and suction pare are finer to get more accurate results. The experimental results showed that in a vertical jet, the lowest mixing time takes place at 35 degree. In addition, mixing time decreased by increasing the Reynolds number. Furthermore, the CFD simulation predicted the items as well a flow patterns precisely that validates the experiments.

Keywords: Jet mixer, CFD, Turbulent model, Nozzle angle, Mixing time, Reynolds Number.

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Authors: D. G. Jung, J. B. Lim, J. H. Kim, J. J. Kim

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The international society focuses on the environment protection and natural energy sources control for the global cooperation against weather change and sustainable growth. The study presents the overview of the water shortage status and the necessity of wastewater reuse facility in military facilities and for the possibility of the introduction, compares the economics by means of cost-benefit analysis. The military features such as the number of users of military barracks and the water use were surveyed by the design principles by facility types, the application method of wastewater reuse facility was selected, the feed water, its application and the volume of reuse volume were defined and the expectation was estimated, confirming the possibility of introducing a wastewater reuse possibility by means of cost-benefit analysis.

Keywords: military barracks, wastewater reuse facility, cost-benefit analysis

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Authors: M. Mydlárová Blaščáková, J. Bernasovská, J. Poráčová, I. Boroňová

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Obesity is frequent attendant phenomenon of patients with endocrinological disease. Between BMI and endocrinological diseases is close correlation. In thesis we focused on the allocation of hormone concentration – PTH and TSH, CHOL a mineral element Ca in a blood serum. The examined group was formed by 100 respondents (women) aged 36 – 83 years, who were divided into two groups – control group (CG), group with diagnosed endocrine disease (DED). The concentration of PTH and TSH, Ca and CHOL was measured through the medium of analyzers Cobas e411 (Japan); Cobas Integra 400 (Switzerland). At individuals was measured body weight as well as stature and thereupon from those data we enumerated BMI. On the basis of Student T-test in biochemical parameter of PTH and Ca we found out significantly meaningful difference (p<0,05) between CG and DED. In CG we made a founding the association between BMI and PTH by means of correlation analysis.

Keywords: Biochemical markers, hormones, obesity, women.

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Authors: Erjola Reufi, Jozefita Marku, Thomas Bier

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Ultrasonic pulse velocity (UPV) method has been shown for some time to provide a reliable means of estimating properties and offers a unique opportunity for direct, quick and safe control of building damaged by earthquake, fatigue, conflagration and catastrophic scenarios. On this investigation hybrid reinforced concrete has been investigated by UPV method. Hooked end steel fiber of length 50 and 30 mm was added to concrete in different proportion 0, 0.25, 0.5, and 1 % by the volume of concrete. On the other hand, polypropylene fiber of length 12, 6, 3 mm was added to concrete of 0.1, 0.2, and 0.4 % by the volume of concrete. Fifteen different mixture has been prepared to investigate the relation between compressive strength and UPV values and also to investigate on the effect of volume and type of fiber on UPV values.

Keywords: Compressive strength, polypropylene fiber, steel fiber, ultrasonic pulse velocity, volume, type of fiber.

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Authors: P. Mohamed Fathimal, P. Arockia Jansi Rani

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In this era of online communication, which transacts data in 0s and 1s, confidentiality is a priced commodity. Ensuring safe transmission of encrypted data and their uncorrupted recovery is a matter of prime concern. Among the several techniques for secure sharing of images, this paper proposes a k out of n region incrementing image sharing scheme for color images. The highlight of this scheme is the use of simple Boolean and arithmetic operations for generating shares and the Lagrange interpolation polynomial for authenticating shares. Additionally, this scheme addresses problems faced by existing algorithms such as color reversal and pixel expansion. This paper regenerates the original secret image whereas the existing systems regenerates only the half toned secret image.

Keywords: Threshold Secret Sharing Scheme, Access Control, Steganography, Authentication, Secret Image Sharing, XOR, Pixel Expansion.

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Authors: M. Rezvani, M. Ahangari, F. Zaefarian

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To study the performance of soybean (Glycine max L.) cultivars in varying weeding regimes, a field experiment was conducted in 2010. The experiment was split plot in a randomized complete block design with 3 replicates. The four cultivars and two lines of soybean including: Sahar, Hill, Sari, Telar, 032 and 033 in main plot and weeding regime consist of no weeding (control), one weeding (35 days after planting) and two weeding (35+20 days after planting) were randomized in sub plot. In weed infested plots inevitably had the highest yield reduction in all varieties. On the other hand, plots weeded twice showed the best performance for all cultivars and lines. Although 033 had the highest yield over weeding regimes, but Hill was the best cultivar in suppression of weeds, which indicated the competitiveness of this cultivar. Double weeding, with the use of competitive soybean cultivars would be an effective approach for producing yield.

Keywords: Biomass, Competition, Density, Weed suppression

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Authors: Su Yi Ming, Hou Ying, Zou Guang Ping

Abstract:

Metal-net rubber is a new dry friction damping material, compared with the traditional metal rubber, which has high mechanization degree, and the mechanical performance of metal-net rubber is more stable. Through the sine sweep experiment and random vibration experiment of metal-net rubber vibration isolator, the influence of several important factors such as the lines slope, relative density and wire diameter on the transfer rate, natural frequency and root-mean-square response acceleration of metal-net rubber vibration isolation system, were studied through the method of control variables. Also, several relevant change curves under different vibration levels were derived, and the effects of vibration level on the natural frequency and root-mean-square response acceleration were analyzed through the curves.

Keywords: Metal-net rubber vibration isolator, relative density, vibration level, wire diameter.

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Authors: Amrita Tripathi, Neeru Adlakha

Abstract:

Calcium [Ca2+] dynamics is studied as a potential form of neuron excitability that can control many irregular processes like metabolism, secretion etc. Ca2+ ion enters presynaptic terminal and increases the synaptic strength and thus triggers the neurotransmitter release. The modeling and analysis of calcium dynamics in neuron cell becomes necessary for deeper understanding of the processes involved. A mathematical model has been developed for cylindrical shaped neuron cell by incorporating physiological parameters like buffer, diffusion coefficient, and association rate. Appropriate initial and boundary conditions have been framed. The closed form solution has been developed in terms of modified Bessel function. A computer program has been developed in MATLAB 7.11 for the whole approach.

Keywords: Laplace Transform, Modified Bessel function, reaction diffusion equation, diffusion coefficient, excess buffer, calcium influx

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Authors: K. Saharan, M.V. R. K. Sarma, A. S. Roesti, A. Prakash, B. N. Johri, M. Aragno, V. S. Bisaria, V. Sahai

Abstract:

Chemically defined Schlegel-s medium was modified to improve production of cell growth and other metabolites that are produced by fluorescent pseudomonad R62 strain. The modified medium does not require pH control as pH changes are kept within ± 0.2 units of the initial pH 7.1 during fermentation. The siderophore production was optimized for the fluorescent pseudomonad strain in the modified medium containing 1% glycerol as a major carbon source supplemented with 0.05% succinic acid and 0.5% Ltryptophan. Indole-3 acetic acid (IAA) production was higher when L-tryptophan was used at 0.5%. The 2,4- diacetylphloroglucinol (DAPG) was higher with amended three trace elements in medium. The optimized medium produced 2.28 g/l of dry cell mass and 900 mg/l of siderophore at the end of 36 h cultivation, while the production levels of IAA and DAPG were 65 mg/l and 81 mg/l respectively at the end of 48 h cultivation.

Keywords: Fluorescent pseudomonad, Fermentation, Metabolites production, PGPR.

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Authors: M. Baghdasaryan, A. Sukiasyan

Abstract:

An algorithm for estimating the stable operation conditions of the synchronous motor of the ore mill electric drive is proposed. The stable operation conditions of the synchronous motor are revealed, taking into account the estimation of the q angle change and the technological factors. The stability condition obtained allows to ensure the stable operation of the motor in the synchronous mode, taking into account the nonlinear character of the mill loading. The developed algorithm gives an opportunity to present the undesirable phenomena, arising in the electric drive system. The obtained stability condition can be successfully applied for the optimal control of the electromechanical system of the mill.

Keywords: Electric drive, synchronous motor, ore mill, stability, technological factors.

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Authors: Wararit Panichkitkosolkul

Abstract:

The unit root tests based on the robust estimator for the first-order autoregressive process are proposed and compared with the unit root tests based on the ordinary least squares (OLS) estimator. The percentiles of the null distributions of the unit root test are also reported. The empirical probabilities of Type I error and powers of the unit root tests are estimated via Monte Carlo simulation. Simulation results show that all unit root tests can control the probability of Type I error for all situations. The empirical power of the unit root tests based on the robust estimator are higher than the unit root tests based on the OLS estimator.

Keywords: Autoregressive, Ordinary least squares, Type I error, Power of the test, Monte Carlo simulation.

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Authors: B. Akturk, N. Yuzer, N. Kabay

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When concrete is exposed to high temperatures, some changes may occur in its physical and mechanical properties. Especially, high strength concrete (HSC), may exhibit damages such as cracks and spallings. To overcome this problem, incorporating polymer fibers such as polypropylene (PP) in concrete is a well-known method. In high temperatures, PP decomposes and releases harmful gases such as CO and CO2. This study researches the use of raw rice husk (RRH) as a sustainable material, instead of PP fibers considering its several favorable properties, and its usability in HSC. RRH and PP fibers were incorporated in concrete at 0.5-3% and 0.2-0.5% by weight of cement, respectively. Concrete specimens were exposed to 20 (control), 300, 600 and 900°C. Under these temperatures, residual compressive and splitting tensile strength was determined. During the high temperature effect, the amount of released harmful gases was measured by a gas detector.

Keywords: Gas analysis, high temperature, high strength concrete, polypropylene fibers, raw rice husk.

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Authors: Hooman Jafarabadi

Abstract:

In this paper application of artificial intelligence for baby and children caring is studied. Then a new idea for injury prevention and safety announcement is presented by using digital image processing. The paper presents the structure of the proposed system. The system determines the possibility of the dangers for children and babies in yards, gardens and swimming pools or etc. In the presented idea, multi camera System is used and receiver videos are processed to find the hazardous areas then the entrance of children and babies in the determined hazardous areas are analyzed. In this condition the system does the programmed action capture, produce alarm or tone or send message.

Keywords: Baby and children Care and Nursing, Intelligent Control Systems for Nursing, Electronic Care and Nursing, Dangers and safety for children and babies, Motion detection, Expert danger alarm systems.

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Authors: Andrey V. Timofeev

Abstract:

This paper introduces an original method for guaranteed estimation of the accuracy for an ensemble of Lipschitz classifiers. The solution was obtained as a finite closed set of alternative hypotheses, which contains an object of classification with probability of not less than the specified value. Thus, the classification is represented by a set of hypothetical classes. In this case, the smaller the cardinality of the discrete set of hypothetical classes is, the higher is the classification accuracy. Experiments have shown that if cardinality of the classifiers ensemble is increased then the cardinality of this set of hypothetical classes is reduced. The problem of the guaranteed estimation of the accuracy for an ensemble of Lipschitz classifiers is relevant in multichannel classification of target events in C-OTDR monitoring systems. Results of suggested approach practical usage to accuracy control in C-OTDR monitoring systems are present.

Keywords: Lipschitz classifiers, confidence set, C-OTDR monitoring, classifiers accuracy, classifiers ensemble.

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Authors: Roza Dastres, Mohsen Soori

Abstract:

Digital Signal Processing (DSP) is the use of digital processing systems by computers in order to perform a variety of signal processing operations. It is the mathematical manipulation of a digital signal's numerical values in order to increase quality as well as effects of signals. DSP can include linear or nonlinear operators in order to process and analyze the input signals. The nonlinear DSP processing is closely related to nonlinear system detection and can be implemented in time, frequency and space-time domains. Applications of the DSP can be presented as control systems, digital image processing, biomedical engineering, speech recognition systems, industrial engineering, health care systems, radar signal processing and telecommunication systems. In this study, advanced methods and different applications of DSP are reviewed in order to move forward the interesting research filed.

Keywords: Digital signal processing, advanced telecommunication, nonlinear signal processing, speech recognition systems.

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Authors: Nazir Ahmad Zafar, Nabeel Sabir, Amir Ali

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Functionalities and control behavior are both primary requirements in design of a complex system. Automata theory plays an important role in modeling behavior of a system. Z is an ideal notation which is used for describing state space of a system and then defining operations over it. Consequently, an integration of automata and Z will be an effective tool for increasing modeling power for a complex system. Further, nondeterministic finite automata (NFA) may have different implementations and therefore it is needed to verify the transformation from diagrams to a code. If we describe formal specification of an NFA before implementing it, then confidence over transformation can be increased. In this paper, we have given a procedure for integrating NFA and Z. Complement of a special type of NFA is defined. Then union of two NFAs is formalized after defining their complements. Finally, formal construction of intersection of NFAs is described. The specification of this relationship is analyzed and validated using Z/EVES tool.

Keywords: Modeling, Nondeterministic finite automata, Znotation, Integration of approaches, Validation.

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Authors: D. S. Mohale, A. V. Chandewar

Abstract:

Aim- Study was undertaken to investigate the effect of altered behavioral condition like depression on various oxidative stress markers and biochemical parameters in rats. Methods- Rats were subjected for short (21 days) and long term (84 days) social isolation; the rats displayed an increase in depression on force swim test relative to control. Various markers of oxidative stress like lipid per oxidation (LPO), reduced glutathione (GSH), Supers oxide dismutase (SOD), catalase (CAT) and biochemical parameters like SGOT, SGPT, and blood glucose were determined. Results- There was significant increase in the level of LPO and decrease in the levels of GSH, SOD and CAT after long term isolation. Biochemical parameters were significantly altered after social isolation. Conclusion- Increased oxidative stress in depression which may leads to alteration of biochemical parameters.

Keywords: Depression, Glucose, LPO, Oxidative stress.

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Authors: Victor A. Naumenko, Yuriy A. Tyulenev, Alla A. Kushch

Abstract:

The objective of current issue was to develop a model of testicular herpes simplex virus (HSV) type I infection for assessment of viral effect on fertility. 56 male mice were inoculated intraperitoneally with different concentrations of HSV on 8 day post partum. It was revealed that the optimal dose was 100 plaque forming units per mice as it provided testicular infection in 100% of survivors. HSV proteins were detected both in somatic and germ cells (spermatogonia, spermatocytes, spermatides). Although DNA load in testis was descending from 3 to 28 days post infection only 12.5% of infected males had offspring after mating with uninfected females comparing to 87.5% in control (p=0.012). These results are the first direct evidence for HSV impact in male sterility. Prepuberal mice appeared to be a suitable model for investigation of pathogenesis of virus-associated fertility disorders.

Keywords: Herpes simplex virus type I, male fertility, prepuberal mice, spermatogenesis.

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