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Effect of Dose Rate of Irradiation on Ultrastructure of Duodenal Mucosa
Abstract:Ultrastructure of duodenum mucosa of irradiated rat was studied versus dose rate of irradiation following exposure to gamma rays from 60-Cobalt source. The animals were whole body irradiated at two dose rates (1 Gy.mn-1 and 1 Gy.h-1) and three total doses (1, 2 or 4 Gy) for each dose rate. 24 or 48 h after irradiation, their small intestine was removed and samples of duodenum were processed for observations under a transmission electron microscopy. Samples of duodenum mucosa of control rats were processed in the same way. For the lower dose rate of 1 Gy.h-1, main lesions characteristic of apoptosis were detected within irradiated enterocytes at a total dose of 2 Gy and 24 h after exposure. Necrosis was noted in the samples, 48 h after exposition. For the higher dose rate of 1 Gy.mn-1, fewer changes were detected at all total doses 24 or 48 h irradiation. Thus, it was shown that the appearance of radiationinduced alterations varies not only with increasing total dose and post-irradiation time but especially with decreasing dose rate.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1054986Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1595
 BD Kavanagh, CC Pan, LA Dawson, SK Das, Li XA, RK Ten Haken, M Miften. "Radiation dose-volume effects in the stomach and small bowel". Int J Radiat Oncol Biol Phys. 2010 vol 76(3 Suppl):S101-7.
 T Bölling, N Willich. "Late effects of abdominal irradiation in children: a review of the literature". Anticancer Res. 2010 vol 30(1):227-31.
 A Huscher, M Bignardi, E Magri, E Vitali, N Pasinetti, L Costa, P Frata,
 Magrini SM. "Determinants of small bowel toxicity in postoperative pelvic irradiation for gynaecological malignancies". Anticancer Res. 2009 vol 29(11):4821-6.
 C Fiorino, R Valdagni, T Rancati, G Sanguineti. "Dose-volume effects for normal tissues in external radiotherapy: pelvis". Radiother Oncol. 2009 vol 93 2):153-67.
 Fiorino C, Alongi F, Perna L, Broggi S, Cattaneo GM, Cozzarini C, Di Muzio N, Calandrino R. "Dose-volume relationships for acute bowel toxicity in patients treated with pelvic nodal irradiation for prostate cancer". Int J Radiat Oncol Biol Phys. 2009 vol 75(1):29-35.
 Hellebust TP, Kristensen GB, Olsen DR. "Late effects after radiotherapy for locally advanced cervical cancer: comparison of two brachytherapy schedules and effect of dose delivered weekly". Int J Radiat Oncol Biol Phys. 2010; vol 76(3):713-8.
 Igaki H, Nakagawa K, Uozaki H, Akahane M, Hosoi Y, Fukayama M, Miyagawa K,Akashi M, Ohtomo K, Maekawa K. "Pathological changes in the gastrointestinal tract of a heavily radiation-exposed worker at the Tokai-mura criticality accident". J Radiat Res (Tokyo). 2008 49 vol (1):55-62.
 Dri├ík D, Osterreicher J, V├ívrov├í J, Reh├íkov├í Z, Vilasov├í Z. "Morphological changes of rat jejunum after whole body gammairradiation and their impact in biodosimetry". Physiol Res. 2008; vol 57(3):475-9. Vyas D, Robertson CM, Stromberg PE, Martin JR, Dunne WM, Houchen CW, Barrett TA, Ayala A, Perl M, Buchman TG, Coopersmith CM. "Epithelial apoptosis in mechanistically distinct methods of injury in the murine small intestine". Histol Histopathol. 2007 22(6):623-30.
 Monobe M, Hino M, Sumi M, Uzawa A, Hirayama R, Ando K, Kojima S. "Protective effects of melatonin on gamma-ray induced intestinal damage". Int J Radiat Biol. 2005 81(11):855-60.
 Walichiewicz P, Przybyszewski WM, Snietura M, Lange D, Bkhiyan A, Widel M. "Protective effect of local temporary ischemia depends on applied dose of radiation". Cancer Lett. 2005;222(1):113-8.
 Wang J, Zheng H, Ou X, Albertson CM, Fink LM, Herbert JM, Hauer- Jensen M. "Hirudin ameliorates intestinal radiation toxicity in the rat: support for thrombin inhibition as strategy to minimize side-effects after radiation therapy and as countermeasure against radiation exposure". J Thromb Haemost. 2004;2(11):2027-35.
 Labéjof LP, Galle P, Mangabeira PA, de Oliveira AH, Severo MI. "Histological changes in rat duodenum mucosa after whole-body gamma irradiation". Cell Mol Biol (Noisy-le-grand). 2002;48(5):537-45.
 Nandchahal K. "Crypt cell population changes in the mouse jejunum during injury and repair after whole-body gamma irradiation". Radiobiol Radiother (Berl). 1990;31(4):337-9.