Victor A. Naumenko and Yuriy A. Tyulenev and Alla A. Kushch
Herpes Simplex Virus Type I Infection of Mice Testis and Effect on Fertility
703 - 706
2012
6
12
International Journal of Medical and Health Sciences
https://publications.waset.org/pdf/1938
https://publications.waset.org/vol/72
World Academy of Science, Engineering and Technology
The objective of current issue was to develop a model
of testicular herpes simplex virus (HSV) type I infection for
assessment of viral effect on fertility. 56 male mice were inoculated
intraperitoneally with different concentrations of HSV on 8 day post
partum. It was revealed that the optimal dose was 100 plaque
forming units per mice as it provided testicular infection in 100 of
survivors. HSV proteins were detected both in somatic and germ
cells (spermatogonia, spermatocytes, spermatides). Although DNA
load in testis was descending from 3 to 28 days post infection only
12.5 of infected males had offspring after mating with uninfected
females comparing to 87.5 in control (p0.012). These results are
the first direct evidence for HSV impact in male sterility. Prepuberal
mice appeared to be a suitable model for investigation of
pathogenesis of virusassociated fertility disorders.
Open Science Index 72, 2012