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Cell Growth and Metabolites Produced by Fluorescent Pseudomonad R62 in Modified Chemically Defined Medium

Authors: K. Saharan, M.V. R. K. Sarma, A. S. Roesti, A. Prakash, B. N. Johri, M. Aragno, V. S. Bisaria, V. Sahai


Chemically defined Schlegel-s medium was modified to improve production of cell growth and other metabolites that are produced by fluorescent pseudomonad R62 strain. The modified medium does not require pH control as pH changes are kept within ± 0.2 units of the initial pH 7.1 during fermentation. The siderophore production was optimized for the fluorescent pseudomonad strain in the modified medium containing 1% glycerol as a major carbon source supplemented with 0.05% succinic acid and 0.5% Ltryptophan. Indole-3 acetic acid (IAA) production was higher when L-tryptophan was used at 0.5%. The 2,4- diacetylphloroglucinol (DAPG) was higher with amended three trace elements in medium. The optimized medium produced 2.28 g/l of dry cell mass and 900 mg/l of siderophore at the end of 36 h cultivation, while the production levels of IAA and DAPG were 65 mg/l and 81 mg/l respectively at the end of 48 h cultivation.

Keywords: Fluorescent pseudomonad, Fermentation, Metabolites production, PGPR.

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[1] E. María and D.M.E. de Villegas, "Biotechnological production of siderophores," in Microbial Siderophores, vol.12, A. Varma, S. Chincholkar, Eds. Berlin, Heidelberg: Springer Verlag, 2007, pp. 219- 231.
[2] S.H. Hua T. Yoshida, Y. Meng, T. Kabashima, K. Ito, Y. Nishiya, Y. Kawamura, and T. Yoshimoto, "Purification and characterization of thermostable glycerol kinase from Thermus flavus," J. Ferment. Bioeng. vol. 83, pp. 328-332, Jan.1997.
[3] D.M.E de Villegas, P. Villa, and A. Frias, "Evaluation of the siderophores production by Pseudomonas aeruginosa PSS," Rev. Latinoam. Microbiol, vol. 44, pp.112-117, Sep. 2002.
[4] T. Leisinger, and L.Margrafet, "Secondary metabolites of the fluorescent pseudomonads," Microbiol Rev, vol. 43, pp. 422-442, Sep. 1979.
[5] D.J. O-Sullivan, and F. O-Gara, "Traits of fluorescent pseudomonas sp. involved in suppression of plant root pathogens," Microbiol. Rev, vol. 56, pp 662-676, Dec.1992.
[6] R. Gaur, S. Noam, S. Kawaljeet, B.N. Johri, P. Rossi, and M. Aragno, "Diacetylphloroglucinol producing pseudomonads do not influence AM fungi in wheat rhizosphere," Curr. Sci, vol. 86, pp 453-457. Feb. 2004.
[7] D. Roesti, R. Gaur, B.N. Johri, G. Imfeld, S. Sharma, K. Kwaljeet, and M. Aragno, "Plant growth stage, fertilizer management and bioinoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields," Soil. Biol. Biochem, vol. 38, pp 1111-1120, Jan. 2006.
[8] D. Rachid, and B. Ahmed, "Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens" African J. Biotechnol, vol. 4, pp 697-702, July 2005.
[9] M. Aragno, and H.G. Schlegel, "The mesophilic hydrogen-oxidizing (Knallgas) bacteria," in The Prokaryotes, A. Balows, H.G. Tr├╝per, M. Dworkin, W. Harder, and K.H. Schleifer KH, Eds. Berlin Heidelberg New York: Springer Verlag, 1991, pp. 344-384.
[10] J.M. Meyer, and M.A. Abdallah, "The florescent pigment of Pseudomonas fluorescens biosynthesis, purification and physicalchemical properties," J Gen Microbiol, vol. 107, pp 319-328, April 1978.
[11] S.A. Gordon and R.P. Weber, "Colorimetric estimation of indole-acetic acid," Plant Physiol, vol. 26, pp. 192-195, Jan. 1951.
[12] B. Schwyn and J.B. Neilands,"Universal Chemical Assay for the detection and determination of siderophores," Anal Biochem, vol. 140, pp. 47-56, Jan. 1987.