Search results for: Nile Tilapia Oreochromis niloticus
31 Effect of Dietary α-Cellulose Levels on the Growth Parameters of Nile Tilapia Oreochromis niloticus Fingerlings
Authors: Keri Alhadi Ighwela, Aziz Bin Ahmad, A. B. Abol-Munafi
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Three purified diets were formulated using fish meal, soya bean, wheat flour, palm oil, minerals and maltose. The carbohydrate in the diets was increased from 5 to 15% by changing the cellulose content to study the effect of dietary carbohydrate level on the growth parameters of Nile tilapia Oreochromis niloticus. The protein and the lipid contents were kept constant in all the diets. The results showed that, weight gain, protein efficiency ratio, net protein utilisation and hepatosomatic index of fish fed the diet containing 15% cellulose were the lowest among all groups. Addition, the fish fed the diet containing 5% cellulose had the best specific growth rate, and food conversion ratio. While, there was no effect of the dietary cellulose levels on condition factor and survival rate. These results indicate that Nile tilapia fingerlings are able to utilize dietary cellulose does not exceed 10% in their feed for optimum growth.Keywords: Dietary cellulose, growth parameters, Nile Tilapia Oreochromis niloticus, purified diets.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 469930 Development of All-male Fingerlings by Heat Treatment and the Genetic Mechanism of Heat Induced Sex Determination in Nile Tilapia(Oreochromis niloticus L.)
Authors: P. O. Angienda, B. O. Aketch, E. N. Waindi
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Juvenile Nile tilapia subjected to heat treatment at temperatures ranging from 260C to 370C showed positive correlation (P<0.01) between treatment temperatures and resultant sex ratios, while, survival rate of the fry showed a negative correlation against temperature (P<0.01). The optimal temperature for both sex shift towards males and survival rates was 36±0.5°C, producing male percentage of 86.31 and a fry survival of 65.25. To determine the genetic basis of temperature sex-determination in Nile tilapia, we employed three microsatellite markers (Abur36, Abur100 and UNH846). Abur36 predicted the sex of 95% of the heat induced individuals, suggesting that the locus influence sex ratio and its interaction with temperature result in male biased sex ratio. This locus could turn out to be the major sex determining gene operating in Nile tilapia. These markers could be used in marker-assisted selection to select genotypes that give a higher percentage of males for commercial production.Keywords: Heat treatment, Microsatellite, Nile tilapia, sex-determination.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 368429 Charaterisation of Salmonella Isolated from Nile Tilapia (Oreochromis niloticus) along Lake Victoria Beaches in Western Kenya
Authors: Wandili S. Awuor, Onyango D. Miruka, Waindi N. Eliud
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Foodborne Salmonella infections have become a major problem world wide. Salmonellosis transmitted from fish are quite common. Established quality control measures exist for export oriented fish, none exists for fish consumed locally. This study aimed at characterization of Salmonella isolated from Nile tilapia . The study was carried out in selected beaches along L. Victoria in Western Kenya between March and June 2007. One hundred and twenty fish specimens were collected. Salmonella isolates were confirmed using serotyping, biochemical testing in addition to malic acid dehydrogenase (mdh) and fliC gene sequencing. Twenty Salmonella isolates were confirmed by mdh gene sequencing. Nine (9) were S. enterica serotype typhimurium, four (4) were S. enterica Serotype, enteritidis and seven (7) were S. enterica serotype typhi. Nile tilapia have a role in transmission of Salmonellosis in the study area, poor sanitation was a major cause of pollution at the beach inshore waters.Keywords: fliC, mdh, Salmonellosis, Serotype
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 198128 Heavy Metal Concentration in Gills and Bones of Tilapia (Oreochromis niloticus) from Jega River, Kebbi State, Nigeria
Authors: D. Y. Bawa, M. I. Ribah, I. S. Jega, V. O. Oyedepo
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A study was conducted to assess some heavy metal concentration (Cadmium (Cd), Copper (Cu), Iron (Fe), Lead (Pb) and Zinc (Zn)) in the gills and bones of Oreochromis niloticus obtained from Jega river. 30 fish samples were collected from March to July 2014 (fortnightly). Bones and gills were used for the assessment of some heavy metals using Atomic Absorption Spectrometer. Results indicated that Pb was not detected in both gills and bones but Fe, Cd, Zn and Cu were present in both the gills and bones of the fish samples. The concentrations of heavy metals in gills were; Fe 3.37±1.10, Cd 0.62±0.08, Zn 6.21±0.11 and Cu 1.28±0.10 mg/kg. The concentrations of heavy metals in bones: Fe 13.08±1.00 mg/kg, Cd 0.99±0.06 mg/kg, Zn 1.28±0.10 mg/kg and Cu 2.23±0.20 mg/kg. The results were found to be within the internationally acceptable standard limits. However, the consumption of small amounts of the identified heavy metals in fish could lead to gradual accumulation over a long period of time and exert toxic effects to consumers. Efforts should be made by the Government to provide appropriate channels for waste disposal to reduce impact on fish.
Keywords: Gills and bones, heavy metal concentration, Jega river, Oreochromis niloticus.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 76127 Physical, Textural and Sensory Properties of Noodles Supplemented with Tilapia Bone Flour (Tilapia nilotica)
Authors: Supatchalee Sirichokworrakit
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Fishbone of Nile Tilapia (Tilapia nilotica), waste from the frozen Nile Tilapia fillet factory, is one of calcium sources. In order to increase fish bone powder value, this study aimed to investigate the effect of Tilapia bone flour (TBF) addition (5, 10, 15% by flour weight) on cooking quality, texture and sensory attributes of noodles. The results indicated that tensile strength, color value (a*) and water absorption of noodles significantly decreased (p£0.05) as the levels of TBF increased from 0-15%. While cooking loss, cooking time and color values (L* and b*) of noodles significantly increased (p£0.05). Sensory evaluation indicated that noodles with 5% TBF received the highest overall acceptability score.
Keywords: Tilapia bone flour, Noodles, Cooking quality, Calcium.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 408826 Determinants of Selenium Intake in a High HIV Prevalence Fishing Community in Bondo District, Kenya
Authors: Samwel Boaz Otieno, Fred Were, Ephantus Kabiru, Kaunda Waza
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A study was done to establish determinants of selenium intake in a high HIV prevalence fishing community in the Pala Bondo district, Kenya. It was established that most of the respondents (61%) were small holder Farmers and Fishermen {χ2 (1, N=386) p<0.000}, and that most of them (91.2%) had up to college level education {χ2.(1, N=386) p<0.000}, while the number of males and females were not significantly different {χ (1, N=386) p=0.263} and 83.5% of respondents were married {χ2 (1, N=386) p=0.000}. The study showed that adults take on average 2.68 meals a day (N=382, SD=0.603), while children take 3.02 meals (N=386, SD=1.031) a day, and that in most households (82.6%) food is prepared by the women {χ2 (1, N=386) p=0.000} and further that 50% of foods eaten in that community are purchased {χ2 (1, N=386)=0.1818, p=0.6698}. The foods eaten by 75.2% of the respondents were Oreochromis niloticus, Lates niloticus, and Sorghum bicolour, 64.1% vegetables and that both children and adults eat same types of food, and further that traditional foods which have become extinct are mainly vegetables (46%). The study established that selenium levels in foods eaten in Pala sub-locations varies with traditional vegetables having higher levels of selenium; for example, Laurnea cornuta (148.5 mg/kg), Cleome gynandra (121.5 mg/kg), Vignia ungulata (21.97 mg/kg), while Rastrineobola argentea (51 mg/kg), Lates niloticus (0), Oreochromis niloticus (0) Sorgum bicolour (19.97 mg/kg), and Sorgum bicolour (0). The study showed that there is an inverse relationship between foods eaten and selenium levels {RR=1.21, p=0.000}, with foods eaten by 75.2% of respondents (Oreochromis niloticus/Lates niloticus) having no detectable selenium. The four soil types identified in the study area had varying selenium levels with pleat loam (13.3 mg/kg), sandy loam (10.7 mg/kg), clay (2.8 mg/kg) and loam (4.8 mg/kg). It was concluded from this study that for the foods eaten by most of the respondents the selenium levels were below Daily Reference Intake.Keywords: Determinants, HIV, food, fishing, selenium.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 127925 Effect of Stocking Density on Monosex Nile Tilapia Growth during Pond Culture in India
Authors: Suman B. Chakraborty, Samir Banerjee
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Stocking density is considered one of the important factors affecting fish growth. But, information related to impact of stocking density on growth performance of monosex tilapia population under the ecological conditions of Gangetic plains in West Bengal, India is limited. The aim of our study was to compare the growth potential of monosex tilapia at various stocking densities and to determine an ideal stocking density for culture of all-male monosex fish. The males were isolated by examination of genital papilla region and were stocked separately in 0.01 ha earthen ponds at different stocking densities (5000, 10000, 15000, 20000, 25000 and 30000 fingerlings/ha). It was found that the highest weight, length, daily weight gain, growth rate and protein content were observed for the 20000 fish/ha density class. Thus, culture of monosex tilapia at a density of 20000 fish/ha can be considered ideal for augmented production of the fish under Indian context.Keywords: Growth potential, Nile tilapia, Pond culture, Stockingdensity.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 585724 Analysis of Fertilizer Effect in the Tilapia Growth of Mozambique (Oreochromis mossambicus)
Authors: Sérgio Afonso Mulema, Andrés Carrión García, Vicente Ernesto
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This paper analyses the effect of fertilizer (organic and inorganic) in the growth of tilapia. An experiment was implemented in the Aquapesca Company of Mozambique; there were considered four different treatments. Each type of fertilizer was applied in two of these treatments; a feed was supplied to the third treatment, and the fourth was taken as control. The weight and length of the tilapia were used as the growth parameters, and to measure the water quality, the physical-chemical parameters were registered. The results show that the weight and length were different for tilapias cultivated in different treatments. These differences were evidenced mainly by organic and feed treatments, where there was the largest and smallest value of these parameters, respectively. In order to prove that these differences were caused only by applied treatment without interference for the aquatic environment, a Fisher discriminant analysis was applied, which confirmed that the treatments were exposed to the same environment condition.Keywords: Fertilizer, tilapia, growth, statistical methods.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 89923 Biokinetics of Coping Mechanism of Freshwater tilapia following Exposure to Waterborne and Dietary Copper
Authors: Jeng-Wei Tsai
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The purpose of this study was to understand the main sources of copper (Cu) accumulation in target organs of tilapia (Oreochromis mossambicus) and to investigate how the organism mediate the process of Cu accumulation under prolonged conditions. By measuring both dietary and waterborne Cu accumulation and total concentrations in tilapia with biokinetic modeling approach, we were able to clarify the biokinetic coping mechanisms for the long term Cu accumulation. This study showed that water and food are both the major source of Cu for the muscle and liver of tilapia. This implied that control the Cu concentration in these two routes will be correlated to the Cu bioavailability for tilapia. We found that exposure duration and level of waterborne Cu drove the Cu accumulation in tilapia. The ability for Cu biouptake and depuration in organs of tilapia were actively mediated under prolonged exposure conditions. Generally, the uptake rate, depuration rate and net bioaccumulation ability in all selected organs decreased with the increasing level of waterborne Cu and extension of exposure duration.Muscle tissues accounted for over 50%of the total accumulated Cu and played a key role in buffering the Cu burden in the initial period of exposure, alternatively, the liver acted a more important role in the storage of Cu with the extension of exposures. We concluded that assumption of the constant biokinetic rates could lead to incorrect predictions with overestimating the long-term Cu accumulation in ecotoxicological risk assessments.Keywords: Biokinetics, Chronic exposure, Copper, Coping mechanism, Tilapia
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 162122 Snails and Fish as Pollution Biomarkers in Lake Manzala and Laboratory B: Lake Manzala Fish
Authors: Hanaa M. M. El-Khayat, Hanan S. Gaber, Hoda Abdel-Hamid, Kadria M. A. Mahmoud, Hoda M. A. Abu Taleb
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This work aimed to examine Oreochromis niloticus fish from Lake Manzala in Port Said, Dakahlya and Damietta governorates, Egypt, as a bio-indicator for the lake water pollution through recording alterations in their hematological, physiological, and histopathological parameters. All fish samples showed a significant increase in levels of alkaline phosphatase (ALP), creatinine and glutathione-S-transferase (GST); only Dakahlya samples showed a significant increase (p<0.01) in aspartate aminotransferase (AST) level and most Dakahlya and Damietta samples showed reversed albumin and globulin ratio and a significant increase in γ-glutamyltransferase (GGT) level. Port-Said and Damietta samples showed a significant decrease of hemoglobin (Hb) while Dakahlya samples showed a significant decrease in white blood cell (WBC) count. Histopathological investigation for different fish organs showed that Port-Said and Dakahlya samples were more altered than Damietta. The muscle and gill followed by intestine were the most affected organs. The muscle sections showed severe edema, neoplasia, necrotic change, fat vacuoles and splitting of muscle fiber. The gill sections showed dilated blood vessels of the filaments, curling of gill lamellae, severe hyperplasia, edema and blood vessels congestion of filaments. The intestine sections revealed degeneration, atrophy, dilation in blood vessels and necrotic changes in sub-mucosa and mucosa with edema in between. The recorded significant alterations, in most of the physiological and histological parameters in O. niloticus samples from Lake Manzala, were alarming for water pollution impacts on lake fish community, which constitutes the main diet and the main source of income for the people inhabiting these areas, and were threatening their public health and economy. Also, results evaluate the use of O. niloticus fish as important bio-indicator for their habitat stressors.
Keywords: Lake Manzala, Oreochromis niloticus fish, water pollution, physiological, hematological and histopathological parameters.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 156021 Evaluation of Water Quality of the Surface Water of the Damietta Nile Branch, Damietta Governorate, Egypt
Authors: M. S. M. El-Bady
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Water quality and heavy metals pollution of the Damietta Nile Branch at Damietta governorate were investigated in the current work. Fourteen different sampling points were selected along the Damietta Nile branch from Ras EL-Bar (sample 1) to Sheremsah (sample 14). Physical and chemical parameters and the concentrations of Cd, Cr, Cu, Ni, Fe, Al, Hg, Pb and Zn were investigated for water quality assessment of Damietta Nile Branch at Damietta Governorate. Most of the samples show that the water is suitable for drinking and irrigation purposes. All locations of samples near the sea are unsuitable water but the samples in the south direction away from the sea are suitable or good water for drinking and irrigation.
Keywords: Water quality indices, Damietta Governorate, Nile River, pollution.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 85620 Variability of Hydrological Modeling of the Blue Nile
Authors: Abeer Samy, Oliver C. Saavedra Valeriano, Abdelazim Negm
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The Blue Nile Basin is the most important tributary of the Nile River. Egypt and Sudan are almost dependent on water originated from the Blue Nile. This multi-dependency creates conflicts among the three countries Egypt, Sudan, and Ethiopia making the management of these conflicts as an international issue. Good assessment of the water resources of the Blue Nile is an important to help in managing such conflicts. Hydrological models are good tool for such assessment. This paper presents a critical review of the nature and variability of the climate and hydrology of the Blue Nile Basin as a first step of using hydrological modeling to assess the water resources of the Blue Nile. Many several attempts are done to develop basin-scale hydrological modeling on the Blue Nile. Lumped and semi distributed models used averages of meteorological inputs and watershed characteristics in hydrological simulation, to analyze runoff for flood control and water resource management. Distributed models include the temporal and spatial variability of catchment conditions and meteorological inputs to allow better representation of the hydrological process. The main challenge of all used models was to assess the water resources of the basin is the shortage of the data needed for models calibration and validation. It is recommended to use distributed model for their higher accuracy to cope with the great variability and complexity of the Blue Nile basin and to collect sufficient data to have more sophisticated and accurate hydrological modeling.
Keywords: Blue Nile Basin, Climate Change, Hydrological Modeling, Watershed.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 307319 Clove Essential Oil Improves Lipid Peroxidation and Antioxidant Activity in Tilapia Fish Fillet Cooked by Grilling and Microwaving
Authors: E. Oskoueian, E. Maroufyan, Y.M. Goh, E. Ramezani-Fard, M. Ebrahimi
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The fish meat plays an important role in the human health as it contains high quality protein. The tilapia fish considered as the third largest group of farmed fish. The oxidative deterioration of fish meat may occur during the cooking process. The proper cooking process and using natural antioxidant to prevent oxidation and enhance the quality of the tilapia fish fillet is necessary. Hence, this research was carried out to evaluate the potential of clove essential oil to prevent lipid peroxidation and enhance the antioxidant activity of tilapia fish fillet cooked using microwaving and grilling methods. The results showed that cooking using microwave significantly (p<0.05) increased the lipid peroxidation and decreased the DPPH and ferric reducing activity power of the fish fillet as compared to grilling method. The fortification of fish fillet using clove essential oil prevented from lipid peroxidation and enhanced the antioxidant activity of the fish fillet significantly (p<0.05). Consequently, fortification of tilapia fish fillet using clove essential oil followed by cooking using griller to have high quality cooked fish meat is recommended.
Keywords: Antioxidant activity, fillet, fish, fortification, lipid peroxidation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 254918 Histopathological Changes in Liver and Muscle of Tilapia Fish from QIRE Exposed to Concentrations of Heavy Metals
Authors: Justina I. R. Udotong
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Toxicity of copper (Cu), lead (Pb) and iron (Fe) to Tilapia guinensis was carried out for 4 days with a view to determining their effects on the liver and muscle tissues. Tilapia guinensis samples of about 10 - 14cm length and 0.2 – 0.4kg weight each were obtained from University of Calabar fish ponds and acclimated for three (3) days before the experimental set up. Survivors after the 96-hr LC50 test period were selected from test solutions of the heavy metals for the histopathological studies. Histological preparations of liver and muscle tissues were randomly examined for histopathological lesions. Results of the histological examinations showed gross abnormalities in the liver tissues due to pathological and degenerative changes compared to liver and muscle tissues from control samples (tilapia fishes from aquaria without heavy metals). Extensive hepatocyte necrosis with chronic inflammatory changes was observed in the liver of fishes exposed to Cu solution. Similar but less damaging effects were observed in the liver of fishes exposed to Pb and Fe. The extent of lesion observed was therefore heavy metal-related. However, no pathologic changes occurred in the muscle tissues.Keywords: Degenerative changes, heavy metal, hepatocyte necrosis, histopathology, toxicity.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 322817 Assessment of Irrigation Practices at Main Irrigation Network in the Nile Delta
Authors: Ahmed Mohsen, Yoshinobu Kitamura, Katsuyuki Shimizu
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The improvement of irrigation systems in the Nile Delta is one of the most important attempts in Egypt to implement more effective irrigation technology by improving the existing irrigation networks. Demand delivery system in the existing irrigation network is using of mechanical gates structures to automatically divert water from one portion of an agricultural field to another in the desired amount and sequence. This paper discusses evaluating main irrigation networks system under the government managed before and after improvement systems in the Nile Delta. The overall results indicate that policy of using the demand delivery concept through irrigation networks is successful by improving water delivery performance among them than the rotation delivery concept that used before. It is provided fair share of water delivery among irrigation districts and available water in the end of irrigation network, although this system located in an end of irrigation networks in the Nile Delta.Keywords: Automation system, Irrigation district, Rotation system, Water delivery performance
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 240416 Establishing Econometric Modeling Equations for Lumpy Skin Disease Outbreaks in the Nile Delta of Egypt under Current Climate Conditions
Authors: Abdelgawad, Salah El-Tahawy
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This paper aimed to establish econometrical equation models for the Nile delta region in Egypt, which will represent a basement for future predictions of Lumpy skin disease outbreaks and its pathway in relation to climate change. Data of lumpy skin disease (LSD) outbreaks were collected from the cattle farms located in the provinces representing the Nile delta region during 1 January, 2015 to December, 2015. The obtained results indicated that there was a significant association between the degree of the LSD outbreaks and the investigated climate factors (temperature, wind speed, and humidity) and the outbreaks peaked during the months of June, July, and August and gradually decreased to the lowest rate in January, February, and December. The model obtained depicted that the increment of these climate factors were associated with evidently increment on LSD outbreaks on the Nile Delta of Egypt. The model validation process was done by the root mean square error (RMSE) and means bias (MB) which compared the number of LSD outbreaks expected with the number of observed outbreaks and estimated the confidence level of the model. The value of RMSE was 1.38% and MB was 99.50% confirming that this established model described the current association between the LSD outbreaks and the change on climate factors and also can be used as a base for predicting the of LSD outbreaks depending on the climatic change on the future.
Keywords: LSD, climate factors, econometric models, Nile Delta.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 96115 Nile Red, an Alternative Fluorescence Method for Quantification of Neutral Lipids in Microalgae
Authors: P. Rattanapoltee, P. Kaewkannetra
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According to biodiesel from microalgae is an attractive fuel for several reasons such as renewable, biodegradable and environmental friendly. Thus, this study, green microalgae Scenedesmus acutus PPNK1 isolated from natural water, was selected based on high growth rates, easy cultivation and high lipid content. The Nile red fluorescence method has been successfully applied to the determination of lipids in S. acutus PPNK1. The combination of the method to the lipid composition in algal cells showed the yellow fluorescence under fluorescent microscope. Interestingly, maximum cell numbers and biomass concentration were obtained at 5.44´107 cells/mL and 1.60 g/L when it was cultivated in BG-11 medium while in case of BG-11 with nitrogen deprivation (N 0.25 g/L), accumulated lipid content in cells (44.67%) was achieved that was higher than that found in case of BG-11 medium at about 2 times (22.63%).
Keywords: Biodiesel, Lipid, Microalgae, Nile red.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 699214 Seismic Inversion to Improve the Reservoir Characterization: Case Study in Central Blue Nile Basin - Sudan
Authors: S. E. Musa, N. E. Mohamed, N. A. Ahmed
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In this study, several crossplots of the P-impedance with the lithology logs (gamma ray, neutron porosity, deep resistivity, water saturation and Vp/Vs curves) were made in three available wells, which were drilled in central part of the Blue Nile basin in depths varies from 1460m to 1600m. These crossplots were successful to discriminate between sand and shale when using PImpedance values, and between the wet sand and the pay sand when using both P-impedance and Vp/Vs together. Also some impedance sections were converted to porosity sections using linear formula to characterize the reservoir in terms of porosity. The used crossplots were created on log resolution, while the seismic resolution can identify only the reservoir, unless a 3D seismic angle stacks were available; then it would be easier to identify the pay sand with great confidence; through high resolution seismic inversion and geostatistical approach when using P-impedance and Vp/Vs volumes.
Keywords: Basin, Blue Nile, Inversion, Seismic.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 247513 Prevalence and Antimicrobial Susceptibility Patterns of Enteric Bacteria Isolated from Water and Fish in Lake Victoria Basin of Western Kenya
Authors: Jackson H. O. Onyuka, Rose Kakai, David M. Onyango, Peter F. Arama, John Gichuki, Ayub V.O. Ofulla
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A cross sectional study design and standard microbiological procedures were used to determine the prevalence and antimicrobial susceptibility patterns of Escherichia coli, Salmonella enterica serovar typhimurium and Vibrio cholerae O1 isolated from water and two fish species Rastrineobola argentea and Oreochromis niloticus collected from fish landing beaches and markets in the Lake Victoria Basin of western Kenya. Out of 162 samples analyzed, 133 (82.1%) were contaminated, with S. typhimurium as the most prevalent (49.6%), followed by E. coli (46.6%), and lastly V. cholerae (2.8%). All the bacteria isolates were sensitive to ciprofloxacin. E. coli isolates were resistant to ampicillin, tetracycline, cotrimoxazole, chloramphenical and gentamicin while S. typhimurium isolates exhibited resistance to ampicillin, tetracycline, and cotrimoxazole. The V. cholerae O1 isolates were resistant to tetracycline and ampicillin. The high prevalence of drug resistant enteric bacteria in water and fish from the study region needs public health intervention from the local government.Keywords: Aquatic environments, Antimicrobial resistance, Enteric bacteria, Lake Victoria Basin
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 254312 Engineering Geological Characteristics of Soil Materials, East Nile Delta, Egypt
Authors: A. I. M. Ismail, N. Ryden
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This paper is concerned with the study of mineralogy and engineering characteristics of soil materials derived from the eastern part of Nile Delta. The clay minerals of the studied soil by using X- ray diffraction are mainly illite (average 72.6 %) and kaolinite (average 2.6 %), expandable portion in illite-smectite mixed layer (average 7 %). Smectite is more abundant in fluviatile clays, whereas kaolinite is more abundant in lagoonal clays. On the other hand, illite and illite-smectite are more abundant in marine clays. The geotechnical results show that the soil under study consists mainly of about 0.3 % gravel, 5 % sand, 51.5 % silt and 42.2 % clay in average. The average shrinkage limit attains 11 % whereas the average value of the plasticity index is 23.4 %. The free swelling ranges from 40 % to 75 % and has a value of 55 % giving an indication about the inadequacy of such soil under foundations. From a construction point of view, the soil under investigation poses many problems even under light foundations due to the swelling and shrinkage. Such swelling and shrinkage is due to the high content of soil materials in the expandable clay minerals of illite and smectite. Based on the results of the present and earlier studies, trial application of soil stabilisation is recommended.Keywords: Engineering Geological Investigations, Nile Delta, Swelling, Shrinkage
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 378511 Probiotic Properties of Lactic Acid Bacteria Isolated from Fermented Food
Authors: Wilailak Siripornadulsil, Siriyanapat Tasaku, Jutamas Buahorm, Surasak Siripornadulsil
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The objectives of this study were to isolate LAB from various sources, dietary supplement, Thai traditional fermented food, and freshwater fish and to characterize their potential as probiotic cultures. Out of 1,558 isolates, 730 were identified as LAB based on isolation on MRS agar supplemented with a bromocresol purple indicator&CaCO3 and Gram-positive, catalase- and oxidase-negative characteristics. Eight isolates showed the potential probiotic properties including tolerance to acid, bile salt & heat, proteolytic, amylolytic & lipolytic activities and oxalate-degrading capability. They all showed the antimicrobial activity against some Gram-negative and Gram-positive pathogenic bacteria. Based on 16S rDNA sequence analysis, they were identified as Enterococcus faecalis BT2 & MG30, Leconostoc mesenteroides SW64 and Pediococcus pentosaceous BD33, CF32, NP6, PS34 & SW5. The health beneficial effects and food safety will be further investigated and developed as a probiotic or protective culture used in Nile tilapia belly flap meat fermentation.
Keywords: Lactic acid bacteria, pathogen, probiotic, protective culture.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 389710 Assessment of Diagnostic Enzymes as Indices of Heavy Metal Pollution in Tilapia Fish
Authors: Justina I. R. Udotong
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Diagnostic enzymes like aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were determined as indices of heavy metal pollution in Tilapia guinensis. Three different sets of fishes treated with lead (Pb), iron (Fe) and copper (Cu) were used for the study while a fourth group with no heavy metal served as a control. Fishes in each of the groups were exposed to 2.65mg/l of Pb, 0.85mg/l of Fe and 0.35 mg/l of Cu in aerated aquaria for 96 hours. Tissue fractionation of the liver tissues was carried out and the three diagnostic enzymes (AST, ALT, and ALP) were estimated. Serum levels of the same diagnostic enzymes were also measured. The mean values of the serum enzyme activity for ALP in each experimental group were 19.5±1.62, 29.67±2.17 and 1.15±0.27 IU/L for Pb, Fe and Cu groups compared with 9.99±1.34 IU/L enzyme activity in the control. This result showed that Pb and Fe caused increased release of the enzyme into the blood circulation indicating increased tissue damage while Cu caused a reduction in the serum level as compared with the level in the control group. The mean values of enzyme activity obtained in the liver were 102.14±6.12, 140.17±2.06 and 168.23±3.52 IU/L for Pb, Fe and Cu groups, respectively compared to 91.20±9.42 IU/L enzyme activity for the control group. The serum and liver AST and ALT activities obtained in Pb, Fe, Cu and control groups are reported. It was generally noted that the presence of the heavy metal caused liver tissues damage and consequent increased level of the diagnostic enzymes in the serum.Keywords: Diagnostic enzymes, enzyme activity, heavy metals, tissues investigations.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 23429 Reconstitute Information about Discontinued Water Quality Variables in the Nile Delta Monitoring Network Using Two Record Extension Techniques
Authors: Bahaa Khalil, Taha B. M. J. Ouarda, André St-Hilaire
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The world economic crises and budget constraints have caused authorities, especially those in developing countries, to rationalize water quality monitoring activities. Rationalization consists of reducing the number of monitoring sites, the number of samples, and/or the number of water quality variables measured. The reduction in water quality variables is usually based on correlation. If two variables exhibit high correlation, it is an indication that some of the information produced may be redundant. Consequently, one variable can be discontinued, and the other continues to be measured. Later, the ordinary least squares (OLS) regression technique is employed to reconstitute information about discontinued variable by using the continuously measured one as an explanatory variable. In this paper, two record extension techniques are employed to reconstitute information about discontinued water quality variables, the OLS and the Line of Organic Correlation (LOC). An empirical experiment is conducted using water quality records from the Nile Delta water quality monitoring network in Egypt. The record extension techniques are compared for their ability to predict different statistical parameters of the discontinued variables. Results show that the OLS is better at estimating individual water quality records. However, results indicate an underestimation of the variance in the extended records. The LOC technique is superior in preserving characteristics of the entire distribution and avoids underestimation of the variance. It is concluded from this study that the OLS can be used for the substitution of missing values, while LOC is preferable for inferring statements about the probability distribution.Keywords: Record extension, record augmentation, monitoringnetworks, water quality indicators.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16118 Optimal use of Climate in the Construction of Traditional Housing as a Greenhouse in Iran
Authors: Emad Hezbkhah, Ebrahim Akhlaghi
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From a long time age, human beings have chosen their place of residence and comfort so that those places would have relatively ideal natural and climatic conditions. For this reason, from the beginning, the civilizations have been formed in the susceptible natural regions such as Mesopotamia in Iran and Nile coasts in Egypt. Also, the core of human density has been made in the form of an oasis in the deserts. Regarding the formation and combination of the native architecture in different regions of Iran, we find that different properties of these climates have affected frequently the formation of cities and the architectural combinations of these regions. Thus, the precise determinations of climatic areas and attaining the climatic properties of different regions have a great deal of importance in presenting appropriate and compatible-with-climate designs.Keywords: climate and architecture, energy Optimizing, Greenhouse
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18567 Evaluation of Some Chemical Parameters as Potential Determinants of Fresh Water Snails with Special Reference to Medically Important Snails in Egypt
Authors: H.M. El-Khayat, N.M. Ismail, K.M. Mahmoud, F.M. Ragb, K.M. El-Said, B. B. Mostafa, F. A. El- Deeb, A.A. Tantawy
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Seasonal survey of freshwater snails in different water courses in Egypt during two successive years included 13 snail species. They represented by Biomphalaria alexandrina, Bulinus truncatus, Physa acuta, Helisoma duryi, Lymnaea natalensis, Planorbis pantries, Cleopatra bulimoides, Lanistes carinatus, Bellamya unicolor, Melanoides tuberculata, Theodoxus niloticus, Succinia cleopatra and Valvata nilotica. B. alexandrina was most abundant during autumn and spring represented by 26and14 snails/site, respectively. B. truncatus was most abundant during winter (7.7and3.6snails/site) of the two years, respectively. L. natalensis was represented by 7snails/site in summer. The tolerance of different snail species to the chemical elements was determined seasonally and correlated to their abundance. In spring, autumn and winter, B. alexandrina was significantly found to live under the highest level of Pb, Cd,Cu, Na, K and Ca concentrations than the other species (p<0.01). Most snail species could be lived at approximately the same concentrations of Na, K and Ca during all seasons.Keywords: chemical parameters, freshwater, snails, tolerance
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16376 Using Low Permeability Sand-Fadr Mixture Membrane for Isolated Swelling Soil
Authors: Mohie Eldin Mohamed Afifiy Elmashad
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Desert regions around the Nile valley in Upper Egypt contain great extent of swelling soil. Many different comment procedures of treatment of the swelling soils for construction such as pre-swelling, load balance OR soil replacement. One of the measure factors which affect the level of the aggressiveness of the swelling soil is the direction of the infiltration water directions within the swelling soils. In this paper a physical model was installed to measure the effect of water on the swelling soil with replacement using fatty acid distillation residuals (FADR) mixed with sand as thick sand-FADR mixture to prevent the water pathway arrive to the swelling soil. Testing program have been conducted on different artificial samples with different sand to FADR contents ratios (4%, 6%, and 9%) to get the optimum value fulfilling the impermeable replacement. The tests show that a FADR content of 9% is sufficient to produce impermeable replacement.Keywords: Swelling soil, FADR, soil improvement, permeability
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18035 Strategies and Compromises: Towards an Integrated Energy and Climate Policy for Egypt
Authors: S.T. El Sheltawy, A. A. Refaat
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Until recently, energy security and climate change were considered separate issues to be dealt with by policymakers. The two issues are now converging, challenging the security and climate communities to develop a better understanding of how to deal with both issues simultaneously. Although Egypt is not a major contributor to the world's total GHG emissions, it is particularly vulnerable to the potential effects of global climate change such as rising sea levels and changed patterns of rainfall in the Nile Basin. Climate change is a major threat to sustainable growth and development in Egypt, and the achievement of the Millennium Development Goals. Egypt-s capacity to respond to the challenges of climate instability will be expanded by improving overall resilience, integrating climate change goals into sustainable development strategies, increasing the use of modern energy systems with reduced carbon intensity, and strengthening international initiatives. This study seeks to establish a framework for considering the complex and evolving links between energy security and climate change, applicable to Egypt.Keywords: climate change, climate policy, cnergy policy, energy security, sustainable development
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 17874 Characterization of the Microbial Induced Carbonate Precipitation Technique as a Biological Cementing Agent for Sand Deposits
Authors: Sameh Abu El-Soud, Zahra Zayed, Safwan Khedr, Adel M. Belal
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The population increase in Egypt is urging for horizontal land development which became a demand to allow the benefit of different natural resources and expand from the narrow Nile valley. However, this development is facing challenges preventing land development and agriculture development. Desertification and moving sand dunes in the west sector of Egypt are considered the major obstacle that is blocking the ideal land use and development. In the proposed research, the sandy soil is treated biologically using Bacillus pasteurii bacteria as these bacteria have the ability to bond the sand partials to change its state of loose sand to cemented sand, which reduces the moving ability of the sand dunes. The procedure of implementing the Microbial Induced Carbonate Precipitation Technique (MICP) technique is examined, and the different factors affecting on this process such as the medium of bacteria sample preparation, the optical density (OD600), the reactant concentration, injection rates and intervals are highlighted. Based on the findings of the MICP treatment for sandy soil, conclusions and future recommendations are reached.
Keywords: Soil stabilization, biological treatment, MICP, sand cementation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10293 Nano-Bioremediation of Contaminated Industrial Wastewater Using Biosynthesized AgNPs and Their Nano-Composite
Authors: Osama M. Darwesh, Sahar H. Hassan, Abd El-Raheem R. El-Shanshoury, Shawky Z. Sabae
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Nanotechnology as multidisciplinary technology is growing rapidly with important applications in several sectors. Also, nanobiotechnology is known for the use of microorganisms for the synthesis of targeted nanoparticles. The present study deals with the green synthesis of silver nanoparticles using aquatic bacteria and the development of a biogenic nanocomposite for environmental applications. 20 morphologically different colonies were isolated from the collected water samples from eight different locations at the Rosetta branch of the Nile Delta, Egypt. The obtained results illustrated that the most effective bacterial isolate (produced the higher amount of AgNPs after 24 h of incubation time) is isolate R3. Bacillus tequilensis was the strongest extracellular bio-manufactory of AgNPs. Biosynthesized nanoparticles had a spherical shape with a mean diameter of 2.74 to 28.4 nm. The antimicrobial activity of silver nanoparticles against many pathogenic microbes indicated that the produced AgNPs had high activity against all tested multi-antibiotic resistant pathogens. Also, the stabilized prepared AgNPs-SA nanocomposite has greater catalytic activity for the decolourization of some dyes like Methylene blue (MB) and Crystal violet. Such results represent a promising stage for producing eco-friendly, cost-effective, and easy-to-handle devices for the bioremediation of contaminated industrial wastewater.
Keywords: Bioremediation, AgNPs, AgNPs-SA nanocomposite, Bacillus tequilensis, nanobiotechnology.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3622 Wadi Halfa Oolitic Ironstone Formation, Wadi Halfa and Argein Areas, North Sudan
Authors: M. Nafi, A. El Amein, M. El Dawi, K. Salih, O. Elbahi, A. Abou
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In present study, a large deposit of oolitic iron ore of Late Carboniferous-Permotriassic-Lower Jurassic age was discovered in Wadi Halfa and Argein areas, North Sudan. It seems that the iron ore mineralization exists in the west and east bank of the River Nile of the study area that are found on the Egyptian-Sudanese border. The Carboniferous-Lower Jurassic age strata were covered by 67 sections and each section has been examined and carefully described. The iron-ore in Wadi Halfa occurs as oolitic ironstone and contained two horizons: (A) horizon and (B) horizon. Only horizon (A) was observed in southern Argein area. The texture of the ore is variable depending on the volume of the component. In thin sections, the average of the ooids was ranged between 90%-80%. The matrix varies between 10%-20% by volume and detritus quartz in other component my reach up to 30% by volume in sandy massive ore. Ooids size ranges from 0.2mm-1.00 mm on average in very coarse ooids may attend up to 1 mm in size. The matrix around the ooids is dominated by iron hydroxide, carbonate, fine, and amorphous silica. The probable ore reserve estimate of 1.234 billion at a head grade of 41.29% Fe for the Wadi Halfa Oolitic Ironstone Formation. The iron ore shows higher content of phosphorus ranges from 6.15% to 0.16%, with mean 1.45%. The new technology Hatch–Ironstone Chloride Segregation (HICS) can be used to produce commercial-quality of iron and reduce phosphorus and silica to acceptable levels for steel industry. The presence of infrastructures in addition to the presence of massive quantities of iron ore would make exploitation economically.Keywords: HICS, Late Carboniferous age, Oolitic iron ore, phosphorus.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2549