Search results for: attitude and orbit control systems

Authors: Bahr Eldin S. M, K. S. Rama Rao, N. Perumal

Abstract:

A generalized unified power quality conditioner (GUPQC) by using three single-phase three-level voltage source converters (VSCs) connected back-to-back through a common dc link is proposed in this paper as a new custom power device for a three-feeder distribution system. One of the converters is connected in shunt with one feeder for mitigation of current harmonics and reactive power compensation, while the other two VSCs are connected in series with the other two feeders to maintain the load voltage sinusoidal and at constant level. A new control scheme based on synchronous reference frame is proposed for series converters. The simulation analysis on compensation performance of GUPQC based on PSCAD/EMTDC is reported.

Keywords: Custom power device, generalized unified power quality conditioner, PSCAD/ETMDC, voltage source converter

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Authors: Alexander Y. Vaninsky

Abstract:

It is suggested to evaluate environmental performance of energy sector using Data Envelopment Analysis with nondiscretionary factors (DEA-ND) with relative indicators as inputs and outputs. The latter allows for comparison of the objects essentially different in size. Inclusion of non-discretionary factors serves separation of the indicators that are beyond the control of the objects. A virtual perfect object comprised of maximal outputs and minimal inputs was added to the group of actual ones. In this setting, explicit solution of the DEA-ND problem was obtained. Energy sector of the United States was analyzed using suggested approach for the period of 1980 – 2006 with expected values of economic indicators for 2030 used for forming the perfect object. It was obtained that environmental performance has been increasing steadily for the period from 7.7% through 50.0% but still remains well below the prospected level

Keywords: DEA with Non Discretionary Factors, Environmental Performance, Energy Sector, Explicit Solution, Perfect Object.

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Authors: Youguo Wang, Lenan Wu

Abstract:

Stochastic resonance (SR) is a phenomenon whereby the signal transmission or signal processing through certain nonlinear systems can be improved by adding noise. This paper discusses SR in nonlinear signal detection by a simple test statistic, which can be computed from multiple noisy data in a binary decision problem based on a maximum a posteriori probability criterion. The performance of detection is assessed by the probability of detection error Per . When the input signal is subthreshold signal, we establish that benefit from noise can be gained for different noises and confirm further that the subthreshold SR exists in nonlinear signal detection. The efficacy of SR is significantly improved and the minimum of Per can dramatically approach to zero as the sample number increases. These results show the robustness of SR in signal detection and extend the applicability of SR in signal processing.

Keywords: Probability of detection error, signal detection, stochastic resonance.

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Authors: O. Pop, C. Barbulescu, M. Nemes, St. Kilyeni

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The tracing methods determine the contribution the power system sources have in their supplying. These methods can be used to assess the transmission prices, but also to recover the transmission fixed cost. In this paper is presented the influence of the modification of commons structure has on the specific price of transfer and on active power losses. The authors propose a power losses allocation method, based on Kirschen-s method. The system operator must make use of a few basic principles about allocation. The only necessary information is the power flows on system branches and the modifications applied to power system buses. In order to illustrate this method, the 25-bus test system is used, elaborated within the Electrical Power Engineering Department, from Timisoara, Romania.

Keywords: Power systems, P-U bus, P-Q bus, loss allocation, traceability methods.

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Authors: Sarah Odofin, Zhiwei Gao, Sun Kai

Abstract:

Operations, maintenance and reliability of wind turbines have received much attention over the years due to the rapid expansion of wind farms. This paper explores early fault diagnosis technique for a 5MW wind turbine system subjected to multiple faults, where genetic optimization algorithm is employed to make the residual sensitive to the faults, but robust against disturbances. The proposed technique has a potential to reduce the downtime mostly caused by the breakdown of components and exploit the productivity consistency by providing timely fault alarms. Simulation results show the effectiveness of the robust fault detection methods used under Matlab/Simulink/Gatool environment.

Keywords: Disturbance robustness, fault monitoring and detection, genetic algorithm and observer technique.

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Authors: Panagiotis Kyratsis, Konstantinos Kakoulis

Abstract:

Nowadays, the rapid development of CAD systems’ programming environments results in the creation of multiple downstream applications, which are developed and becoming increasingly available. CAD based manufacturing simulations is gradually following the same trend. Drilling is the most popular holemaking process used in a variety of industries. A specially built piece of software that deals with the drilling kinematics is presented. The cutting forces are calculated based on the tool geometry, the cutting conditions and the tool/work-piece materials. The results are verified by experimental work. Finally, the response surface methodology (RSM) is applied and mathematical models of the total thrust force and the thrust force developed because of the main cutting edges are proposed.

Keywords: Application programming interface, CAD, drilling, response surface methodology, RSM.

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Authors: Ahmad Khalesizadeh, Alireza Vakili, Mohsen Danesh Mesgaran, Reza Valizadeh

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The objective of this study was to determine the effects of garlic oil (Allium sativa), turmeric powder (Curcuma longa Linn) and Monensin on Total apparent digestibility of nutrients in Baloochi lambs. The experiment was designed as a 4 x 4 Latin square using 4 ruminally baloochi lambs with 4 treatments in four 28-d periods. Treatments were control (no additive), garlic oil (0. 4 g/d), monensin (0. 2 g/d) and turmeric powder (20 g/d). Total apparent digestibility's (% of intake) of organic matter (OM), dry matter (DM), crud protein (CP), ether extract(EE), non fiber carbohydrate (NFC), acid detergent fiber (ADF) and neutral detergent fiber (NDF) in the total tract were not influenced by addition of either additives.

Keywords: apparent digestibility, essential oil, garlic oil, monensin, turmeric

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Authors: Nopparat Pochai

Abstract:

Smoke discharging is a main reason of air pollution problem from industrial plants. The obstacle of a building has an affect with the air pollutant discharge. In this research, a mathematical model of the smoke dispersion from two sources and one source with a structural obstacle is considered. The governing equation of the model is an isothermal mass transfer model in a viscous fluid. The finite element method is used to approximate the solutions of the model. The triangular linear elements have been used for discretising the domain, and time integration has been carried out by semi-implicit finite difference method. The simulations of smoke dispersion in cases of one chimney and two chimneys are presented. The maximum calculated smoke concentration of both cases are compared. It is then used to make the decision for smoke discharging and air pollutant control problems on industrial area.

Keywords: Air pollution, Smoke dispersion, Finite element method, Stream function, Vorticity equation, Convection-diffusion equation, Semi-implicit method

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Authors: P. Ghasemi Dehkordi, A. Doosti, M. R. Hajimirzaei

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TTV is an unenveloped circular single-stranded DNA virus with a diameter of 30-32 nm that first was described in 1997 in Japan. TTV was detected in various populations without proven pathology, including blood donors and in patients with chronic HBV and HCV hepatitis. The aim of this study was to determine the prevalence of TTV DNA in Iranian patients with chronic hepatitis B and C. Viral TTV-DNA was studied in 442 samples (202 with HBV, 138 with HCV and 102 controls) collected from west south of Iran. All extracted serum DNA was amplified by TTV ORF1 gene specific primers using the semi nested PCR technique. TTV DNA was detected in the serum of 8.9% and 10.8% patients with chronic hepatitis B and C, respectively. Prevalence of TTV-DNA in the serum of 102 controls was 2.9%. Results showed significant relation of TTV with HBV and HCV in patients by using T test examination (P<0.01). The prevalence of TTV-DNA in Iranian hepatitis B and C patients is rather high, and compare with other countries. To control and prevention of the distribution of TT-virus, examination of the blood and blood products it seems to be necessary.

Keywords: Transfusion-transmitted virus (TTV), Hepatitis Cvirus (HCV), Hepatitis B virus (HBV), ORF1 gene, Semi nested PCR, Iran.

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Authors: S. Falkenberg, L. Overmeyer

Abstract:

The “conveyor belt" as a product represents a complex high performance component with a wide range of different applications. Further development of these highly complex components demands an integration of new technologies and new enhanced materials. In this context nanostructured fillers appear to have a more promising effect on the performance of the conveyor belt composite than conventional micro-scaled fillers. Within the project “DotTrans" nanostructured fillers, for example silicon dioxide, are used to optimize performance parameters of conveyor belt systems. The objective of the project includes operating parameters like energy consumption or friction characteristics as well as adaptive parameters like cut or wear resistance.

Keywords: Conveyor belt, nanostructured fillers, wear resistance, friction characteristics.

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Authors: Antonio Di Leva, Carla Reyneri, Michele Sonnessa

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PARADIGMA (PARticipative Approach to DIsease Global Management) is a pilot project which aims to develop and demonstrate an Internet based reference framework to share scientific resources and findings in the treatment of major diseases. PARADIGMA defines and disseminates a common methodology and optimised protocols (Clinical Pathways) to support service functions directed to patients and individuals on matters like prevention, posthospitalisation support and awareness. PARADIGMA will provide a platform of information services - user oriented and optimised against social, cultural and technological constraints - supporting the Health Care Global System of the Euro-Mediterranean Community in a continuous improvement process.

Keywords: Decision Support Systems, Ontology, Healt Care, Clinical Pathway

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Authors: Peña Aguilar Juan M., Arriaga Barrera H., Velázquez Alejos Miguel, Genis Ernesto, Valencia Pérez L. R., Bermúdez Peña M. Carmen

Abstract:

To give a general view of the innovation environments is the aim of this paper, we pretend to make an analysis between Mexico and BRICS (Brazil, Russia, India, China and South Africa- countries belonging to the group of five major emerging economies). The comparison takes by reference a set of various indicators that directly or indirectly affect innovation in a positive or negative way. Firstly, a research to obtain the values of each of the indicators was conducted, considering the main primary sources, then, within a set of radial charts is presented the resulting values of each nation and a comparison between them. Finally, a description of the gaps between Mexico and the BRICS were established, including the areas of opportunity for Mexico

Keywords: Innovation, triple-helix, innovation systems, indicators.

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Authors: Hossein Mohammadpour, Rahman Dashti

Abstract:

Power transformers are among the most important and expensive equipments in the electric power systems. Consequently the transformer protection is an essential part of the system protection. This paper presents a new method for locating transformer winding faults such as turn-to-turn, turn-to-core, turn-totransformer body, turn-to-earth, and high voltage winding to low voltage winding. In this study the current and voltage signals of input and output terminals of the transformer are measured, which the Fourier transform of measured signals and harmonic analysis determine the fault's location.

Keywords: turn-to-turn faults, short circuit, Fourier transform, harmonic analysis.

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Authors: S. Liangwongsan, A. Oonsivilai

Abstract:

Nowadays, hard disk is one of the most popular storage components. In hard disk industry, the hard disk drive must pass various complex processes and tested systems. In each step, there are some failures. To reduce waste from these failures, we must find the root cause of those failures. Conventionall data analysis method is not effective enough to analyze the large capacity of data. In this paper, we proposed the Hough method for straight line detection that helps to detect straight line defect patterns that occurs in hard disk drive. The proposed method will help to increase more speed and accuracy in failure analysis.

Keywords: Hough Transform, Failure Analysis, Media, Hard Disk Drive

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Authors: U. Ramesh Babu, V. Vijay Kumar Reddy, S. Tara Kalyani

Abstract:

The growth in the demand of electrical energy is leading to load on the Power system which increases the occurrence of frequent oscillations in the system. The reason for the oscillations is due to the lack of damping torque which is required to dominate the disturbances of Power system. By using FACT devices, such as Unified Power Flow Controller (UPFC) can control power flow, reduce sub-synchronous resonances and increase transient stability. Hence, UPFC is used to damp the oscillations occurred in Power system. This research focuses on adapting the neuro fuzzy controller for the UPFC design by connecting the infinite bus (SMIB - Single machine Infinite Bus) to a linearized model of synchronous machine (Heffron-Phillips) in the power system. This model gains the capability to improve the transient stability and to damp the oscillations of the system.

Keywords: Power System, UPFC, (ANFIS) Adaptive Neuro Fuzzy Inference System, transient, Low frequency oscillations.

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Authors: Wang, Kuei-Ing, Jou-Fan Shih

Abstract:

This study adopts deterrence theory as well as social identities as moderators, and explores their moderating affects on online toxic disinhibition. Survey and Experimental methodologies are applied to test the research model and four hypotheses are developed in this study. The controllability of identity positively influenced the behavior of toxic disinhibition both in experimental and control groups while the fluidity of the identity did not have significant influences on online disinhibition. Punishment certainty, punishment severity as well as social identity negatively moderated the relation between the controllability of the identity and the toxic disinhibition. The result of this study shows that internet users hide their real identities when they behave inappropriately on internet, but once they acknowledge that the inappropriate behavior will be found and punished severely, the inappropriate behavior then will be weakened.

Keywords: Seductive properties of Internet, Online Disinhibition, Punishment Certainty, Punishment Severity, Social Identity.

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Authors: Yi Hong, Zhao Yang Dong

Abstract:

This paper presents the performance analysis of space-time trellis codes in orthogonal frequency division multiplexing systems (STTC-OFDMs) over quasi-static frequency selective fading channels. In particular, the effect of channel delay distributions on the code performance is discussed. For a STTCOFDM over multiple-tap channels, two extreme conditions that produce the largest minimum determinant are highlighted. The analysis also proves that the corresponding coding gain increases with the maximum tap delay. The performance of STTC-OFDM, under various channel conditions, is evaluated by simulation. It is shown that the simulation results agree with the performance analysis.

Keywords: Space-time trellis code, OFDM, delay profile.

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Authors: Tadeusz Witkowski, Paweł Antczak, Arkadiusz Antczak

Abstract:

In the paper we discuss the influence of the route flexibility degree, the open rate of operations and the production type coefficient on makespan. The flexible job-open shop scheduling problem FJOSP (an extension of the classical job shop scheduling) is analyzed. For the analysis of the production process we used a hybrid heuristic of the GRASP (greedy randomized adaptive search procedure) with simulated annealing algorithm. Experiments with different levels of factors have been considered and compared. The GRASP+SA algorithm has been tested and illustrated with results for the serial route and the parallel one.

Keywords: Makespan, open shop, route flexibility, serial and parallel route, simulation modeling, type of production.

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Authors: Ching-Huei Lin, Yao-Pang Hsu, M. Z. Dosaev, Yu. D. Selyutskii, L. A. Klimina

Abstract:

Hysteresis phenomenon has been observed in the operations of both horizontal-axis and vertical-axis wind turbines (HAWTs and VAWTs). In this study, wind tunnel experiments were applied to investigate the characters of hysteresis phenomena between the angular speed and the external resistance of electrical loading during the operation of a Darrieus type VAWT. Data of output voltage, output current, angular speed of wind turbine under different wind speeds are measured and analyzed. Results show that the range of external resistance changes with the wind speed. The range decreases as the wind speed increases following an exponential decay form. Experiments also indicate that the maximum output power of wind turbines is always inside the range where hysteresis happened. These results provide an important reference to the design of output control system of wind turbines.

Keywords: Hysteresis phenomenon, Angular speed, Range ofexternal resistance

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Authors: Seyed Alireza Valadabadi, Hossein Aliabadi Farahani

Abstract:

This experiment was carried out to study the effect of AMF, drought stress and phosphorus on physiological growth indices of basil at Iran using by a split-plot design with three replications. The main-plot factor included: two levels of irrigation regimes (control=no drought stress and irrigation after 80 evaporation= drought stress condition) while the sub-plot factors included phosphorus (0, 35 and 70 kg/ha) and application and non-application of Glomus fasciculatum. The results showed that total dry matter (TDM), life area index (LAI), relative growth rate (RGR) and crop growth rate (CGR) were all highly significantly different among the phosphorus, whereas drought stress had effect of practical significance on TDM, LAI, RGR and CGR. The results also showed that the highest TDM, LAI, RGR and CGR were obtained from application of Glomus fasciculatum under no-drought condition.

Keywords: Drought stress, Glomus fasciculatum, physiological growth indices

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Authors: Michal M. Koziol

Abstract:

The article presents test results on the changes occurring in sewage sludge during the process of its storage. Tests were conducted on mechanically dehydrated sewage sludge derived from large municipal sewage treatment plants equipped with biological sewage treatment systems. In testing presented in the paper the focus was on the basic fuel properties of sewage sludge: moisture content, heat of combustion, carbon share. In the first part of the article the overview of the issues concerning the sewage sludge management is presented and the genesis of tests is explained. Further in the paper, selected results of conducted tests are discussed. Changes in tested parameters were determined in the period of a 10- month sewage storage.

Keywords: fuel properties, laboratory tests, sewage sludge, storage

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Authors: Yen-Ting Wang, Pao-Cheng Lin, Chen-Fu Huang, Lung-Ching Liang, Alex J.Y. Lee

Abstract:

This study examined the effects of 8-week Pilates training program on limits of stability (LOS) and abdominal muscle strength in young dancers. Twenty-four female volunteered and randomly assigned as experimental group (EG) or control group (CG). All subjects received the same dance lessons but the EG underwent an extra Pilates mat exercises for 40 minutes, three times a week, for 8 weeks. LOS was evaluated by the Biodex Balance System and the abdominal strength was measured by 30/60 seconds sit-ups test. One factor ANCOVA was used to examine the differences between groups after training. The results showed that the overall LOS scores at levels 2/8 and the 30/60 seconds sit-ups for the EG group pre- and post-training were changed from 22/38 % to 31/51 % and 20/33 times to 24/42 times, respectively. The study demonstrated that 8-week Pilates training can improve the LOS performance and abdominal strength in young dancers.

Keywords: Balance, Core Strength Exercise Training, and Posture Stability.

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Authors: Mahmud Fotuhi-Firuzabad, Saeed Afshar

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This paper presents the results of a preventive maintenance application-based study and modeling of failure rates in breakers of electrical distribution systems. This is a critical issue in the reliability assessment of a system. In the analysis conducted in this paper, the impacts of failure rate variations caused by a preventive maintenance are examined. This is considered as a part of a Reliability Centered Maintenance (RCM) application program. A number of load point reliability indices is derived using the mathematical model of the failure rate, which is established using the observed data in a distribution system.

Keywords: Reliability-Centered Maintenance (RCM), failure rate, preventive maintenance (PM), Distribution System Reliability.

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Authors: Siamak Rasulzadeh

Abstract:

Graph transformation has recently become more and more popular as a general visual modeling language to formally state the dynamic semantics of the designed models. Especially, it is a very natural formalism for languages which basically are graph (e.g. UML). Using this technique, we present a highly understandable yet precise approach to formally model and analyze the behavioral semantics of UML 2.0 Activity diagrams. In our proposal, AGG is used to design Activities, then using our previous approach to model checking graph transformation systems, designers can verify and analyze designed Activity diagrams by checking the interesting properties as combination of graph rules and LTL (Linear Temporal Logic) formulas on the Activities.

Keywords: UML 2.0 Activity, Verification, Model Checking, Graph Transformation, Dynamic Semantics.

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Authors: Abdur Rosyid, Mohamed El-Madany, Mohanad Alata

Abstract:

Due to simplicity and low cost, rotordynamic system is often modeled by using lumped parameters. Recently, finite elements have been used to model rotordynamic system as it offers higher accuracy. However, it involves high degrees of freedom. In some applications such as control design, this requires higher cost. For this reason, various model reduction methods have been proposed. This work demonstrates the quality of model reduction of rotor-bearing-support system through substructuring. The quality of the model reduction is evaluated by comparing some first natural frequencies, modal damping ratio, critical speeds, and response of both the full system and the reduced system. The simulation shows that the substructuring is proven adequate to reduce finite element rotor model in the frequency range of interest as long as the number and the location of master nodes are determined appropriately. However, the reduction is less accurate in an unstable or nearly-unstable system.

Keywords: Finite element model, rotordynamic system, model reduction, substructuring.

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Authors: Aurelian Octavian Ciucâ, Ioan Bitir-Istrate, Mircea Scripcariu

Abstract:

The rapidly increasing costs of power line extensions and fossil fuel, combined with the desire to reduce carbon dioxide emissions pushed the development of hybrid power system suited for remote locations, the purpose in mind being that of autonomous local power systems. The paper presents the suggested solution for a “high penetration" hybrid power system, it being determined by the location of the settlement and its “zero policy" on carbon dioxide emissions. The paper focuses on the technical solution and the power flow management algorithm of the system, taking into consideration local conditions of development.

Keywords: Renewable energy, hybrid power system, wind turbine, photovoltaic panels, bio-diesel cogeneration, bio-fuel.

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Authors: M. Pervan, T. Pavic Kramaric

Abstract:

The aim of this paper is to investigate the influence of market share and diversification on the nonlife insurers- performance. The underlying relationships have been investigated in different industries and different disciplines (economics, management...), still, no consistency exists either in the magnitude or statistical significance of the relationship between market share (and diversification as well) on one side and companies- performance on the other side. Moreover, the direction of the relationship is also somewhat questionable. While some authors find this relationship to be positive, the others reveal its negative association. In order to test the influence of market share and diversification on companies- performance in Croatian nonlife insurance industry for the period from 1999 to 2009, we designed an empirical model in which we included the following independent variables: firms- profitability from previous years, market share, diversification and control variables (i.e. ownership, industrial concentration, GDP per capita, inflation). Using the two-step generalized method of moments (GMM) estimator we found evidence of a positive and statistically significant influence of both, market share and diversification, on insurers- profitability.

Keywords: Diversification, market share, nonlife insurance

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Authors: Tatsuya Kasuga, Hidehisa Shimada, Kimio Oguchi

Abstract:

Recent rapid progress in ICT (Information and Communication Technology) has advanced the penetration of sensor networks (SNs) and their attractive applications. Agriculture is one of the fields well able to benefit from ICT. Plant factories control several parameters related to plant growth in closed areas such as air temperature, humidity, water, culture medium concentration, and artificial lighting by using computers and AI (Artificial Intelligence) is being researched in order to obtain stable and safe production of vegetables and medicinal plants all year anywhere, and attain self-sufficiency in food. By providing isolation from the natural environment, a plant factory can achieve higher productivity and safe products. However, the biggest issue with plant factories is the return on investment. Profits are tenuous because of the large initial investments and running costs, i.e. electric power, incurred. At present, LED (Light Emitting Diode) lights are being adopted because they are more energy-efficient and encourage photosynthesis better than the fluorescent lamps used in the past. However, further cost reduction is essential. This paper introduces experiments that reveal which color of LED lighting best enhances the growth of cultured radish sprouts. Radish sprouts were cultivated in the experimental environment formed by a hydroponics kit with three cultivation shelves (28 samples per shelf) each with an artificial lighting rack. Seven LED arrays of different color (white, blue, yellow green, green, yellow, orange, and red) were compared with a fluorescent lamp as the control. Lighting duration was set to 12 hours a day. Normal water with no fertilizer was circulated. Seven days after germination, the length, weight and area of leaf of each sample were measured. Electrical power consumption for all lighting arrangements was also measured. Results and discussions: As to average sample length, no clear difference was observed in terms of color. As regards weight, orange LED was less effective and the difference was significant (p < 0.05). As to leaf area, blue, yellow and orange LEDs were significantly less effective. However, all LEDs offered higher productivity per W consumed than the fluorescent lamp. Of the LEDs, the blue LED array attained the best results in terms of length, weight and area of leaf per W consumed. Conclusion and future works: An experiment on radish sprout cultivation under 7 different color LED arrays showed no clear difference in terms of sample size. However, if electrical power consumption is considered, LEDs offered about twice the growth rate of the fluorescent lamp. Among them, blue LEDs showed the best performance. Further cost reduction e.g. low power lighting remains a big issue for actual system deployment. An automatic plant monitoring system with sensors is another study target.

Keywords: Electric power consumption, LED color, LED lighting, plant factory.

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Authors: A. C. B. C. J. Fernandes, V. C. de Jesus, S. Noruziaan, O. F. G. G. Vilela, K. K. Somarin, R. França, F. H. S. L. Pinheiro

Abstract:

Objective: This in vitro study aimed to evaluate the shear bond strength (SBS) of orthodontic tubes after different enamel pre-treatments. Materials and Methods: A total of 39 crown halves were randomly divided into 3 groups (n = 13). Group I (control group) was exposed to prophy paste (PP), 37% phosphoric acid (PA), and a self-etching primer (SEP). Group II received no prophylaxis, but only PA and SEP. Group III was exposed to PP and SEP. The SBS was used to evaluate the bond strength of the orthodontic tubes one year after bonding. One-way ANOVA and Tukey’s post-hoc test were used to compare SBS values between the three groups. The statistical significance was set to 5%. Results: The difference in SBS values of groups I (36.672 ± 9.315 Mpa), II (34.242 ± 9.986 Mpa), and III (39.055 ± 5.565 Mpa) were not statistically significant (P < 0.05). Conclusion: This study suggests that chairside time can be significantly reduced with the use of PP and a SEP without compromising adhesion. Further evidence is needed by means of a split-mouth design trial.

Keywords: Shear bond strength, orthodontic tubes, self-etching primer, pumice, prophy.

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Authors: Rezvan Najafi, Korosh Heydari, Massoud Saidijam

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5-FU is a chemotherapeutic agent that has been used in colorectal cancer (CRC) treatment. However, it is usually associated with the acquired resistance, which decreases the therapeutic effects of 5-FU. miR-200c is involved in chemotherapeutic drug resistance, but its mechanism is not fully understood. In this study, the effect of inhibition of miR-200c in sensitivity of HCT-116 CRC cells to 5-FU was evaluated. HCT-116 cells were transfected with LNA-anti- miR-200c for 48 h. mRNA expression of miR-200c was evaluated using quantitative real- time PCR. The protein expression of phosphatase and tensin homolog (PTEN) and E-cadherin were analyzed by western blotting. Annexin V and propidium iodide staining assay were applied for apoptosis detection. The caspase-3 activation was evaluated by an enzymatic assay. The results showed LNA-anti-miR-200c inhibited the expression of PTEN and E-cadherin protein, apoptosis and activation of caspase 3 compared with control cells. In conclusion, these results suggest that miR-200c as a prognostic marker can overcome to 5-FU chemoresistance in CRC.

Keywords: Colorectal cancer, miR-200c, 5-FU resistance, E-cadherin, PTEN.

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