Search results for: Atomic force microscopy
653 Iron Doped Biomaterial Calcium Borate: Synthesis and Characterization
Authors: G. Çelik Gül, F. Kurtuluş
Abstract:
Colemanite is the most common borate mineral, and the main source of the boron required by plants, human, and earth. Transition metals exhibit optical and physical properties such as; non-linear optical character, structural diversity, thermal stability, long cycle life and luminescent radiation. The doping of colemanite with a transition metal, bring it very interesting and attractive properties which make them applicable in industry. Iron doped calcium borate was synthesized by conventional solid state method at 1200 °C for 12 h with a systematic pathway. X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy/energy dispersive analyze (SEM/EDS) were used to characterize structural and morphological properties. Also, thermal properties were recorded by thermogravimetric-differential thermal analysis (TG/DTA).
Keywords: Colemanite, conventional synthesis, powder x-ray diffraction, borates.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1005652 Numerical Simulation of Interfacial Flow with Volume-Of-Fluid Method
Authors: Afshin Ahmadi Nadooshan
Abstract:
In this article, various models of surface tension force (CSF, CSS and PCIL) for interfacial flows have been applied to dynamic case and the results were compared. We studied the Kelvin- Helmholtz instabilities, which are produced by shear at the interface between two fluids with different physical properties. The velocity inlet is defined as a sinusoidal perturbation. When gravity and surface tension are taking into account, we observe the development of the Instability for a critic value of the difference of velocity of the both fluids. The VOF Model enables to simulate Kelvin-Helmholtz Instability as dynamic case.
Keywords: Interfacial flow, Incompressible flow, surface tension, Volume-Of-Fluid, Kelvin-Helmholtz.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2560651 Application of Ti/RuO2-SnO2-Sb2O5 Anode for Degradation of Reactive Black-5 Dye
Authors: Jayesh P. Ruparelia, Bhavna D. Soni
Abstract:
Electrochemical-oxidation of Reactive Black-5 (RB- 5) was conducted for degradation using DSA type Ti/RuO2-SnO2- Sb2O5 electrode. In the study, for electro-oxidation, electrode was indigenously fabricated in laboratory using titanium as substrate. This substrate was coated using different metal oxides RuO2, Sb2O5 and SnO2 by thermal decomposition method. Laboratory scale batch reactor was used for degradation and decolorization studies at pH 2, 7 and 11. Current density (50mA/cm2) and distance between electrodes (8mm) were kept constant for all experiments. Under identical conditions, removal of color, COD and TOC at initial pH 2 was 99.40%, 55% and 37% respectively for initial concentration of 100 mg/L RB-5. Surface morphology and composition of the fabricated electrode coatings were characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) respectively. Coating microstructure was analyzed by X-ray diffraction (XRD). Results of this study further revealed that almost 90% of oxidation occurred within 5-10 minutes.
Keywords: Electrochemical-oxidation, RB- dye, Decolorization.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2684650 Topical Delivery of Thymidine Dinucleotide to Induce p53 Generation in the Skin by Elastic Liposome
Authors: Yi-Ping Fang, Yi-Ting Wong
Abstract:
Transcription factor p53 has a powerful tumor suppressing function that is associated with many cancers. However, p53 of the molecular weight was higher make the limitation across to skin or cell membrane. Thymidine dinucleotide (pTT), an oligonucleotide, can activate the p53 transcription factor. pTT is a hydrophilic and negative charge oligonucleotide, which delivery in to cell membrane need an appropriate carrier. The aim of this study was to improve the bioavailability of the nucleotide fragment, thymidine dinucleotide (pTT), using elasic liposome carriers to deliver the drug into the skin. The study demonstrate that dioleoylphosphocholine (DOPC) incorporated with sodium cholate at molar ratio 1:1 can archived the particle size about 220 nm. This elastic liposome could penetration through skin from stratum corneum to whole epidermis by confocal laser scanning microscopy (CLSM). Moreover, we observed the the slight increase in generation of p53 by western blot.Keywords: Elastic liposome, Tymidine dinucleotide, p53, Topical delivery
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1836649 MHD Mixed Convection in a Vertical Porous Channel
Authors: B. Fersadou, H. Kahalerras
Abstract:
This work deals with the problem of MHD mixed convection in a completely porous and differentially heated vertical channel. The model of Darcy-Brinkman-Forchheimer with the Boussinesq approximation is adopted and the governing equations are solved by the finite volume method. The effects of magnetic field and buoyancy force intensities are given by the Hartmann and Richardson numbers respectively, as well as the Joule heating represented by Eckert number on the velocity and temperature fields, are examined. The main results show an augmentation of heat transfer rate with the decrease of Darcy number and the increase of Ri and Ha when Joule heating is neglected.Keywords: Heat sources, magnetic field, mixed convection, porous channel.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2582648 Critical Analysis of Different Actuation Techniques for a Micro Cantilever
Authors: B. G. Sheeparamatti, Prashant Hanasi, Vanita Abbigeri
Abstract:
The objective of this work is to carryout critical comparison of different actuation mechanisms like electrostatic, thermal, piezoelectric, and magnetic with reference to a micro cantilever. The relevant parameters like force generated, displacement are compared in actuation methods. With these results, helps in choosing the best actuation method for a particular application. In this study, Comsol/Multiphysics software is used. Modeling and simulation is done by considering the micro cantilever of same dimensions as an actuator using all the above mentioned actuation techniques. In addition to their small size, micro actuators consume very little power and are capable of accurate results. In this work, a comparison of actuation mechanisms is done to decide the efficient system in micro domain.Keywords: Actuation techniques, microswitch, micro actuator, microsystems.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2419647 Investigation of Mg and Zr Addition on the Mechanical Properties of Commercially Pure Al
Authors: Samiul Kaiser, M. S. Kaiser
Abstract:
The influence of Mg and Zr addition on mechanical properties such as hardness, tensile strength and impact energy of commercially pure Al are investigated. The microstructure and fracture behavior are also studied by using Optical and Scanning Electron Microscopy. It is observed that magnesium addition improves the mechanical properties of commercially pure Al at the expense of ductility due to formation of β″ (Al3Mg) and β′ (Al3Mg2) phase into the alloy. Zr addition also plays a positive role through grain refinement effect and the formation of metastable L12 Al3Zr precipitates. In addition, it is observed that the fractured surface of Mg added alloy is brittle and higher numbers of dimples are observed in case of Zr added alloy.
Keywords: Al-alloys, hardness, tensile strength, impact energy, microstructure.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 675646 Analyzing and Determining the Ideal Response Force for Combatting Terrorist Groups
Authors: Erhan Turgut, Salih Ergün, Abdülkadir Öz
Abstract:
Terror is a modern war strategy which uses violence as a means of communication in order to achieve political objectives. In today’s security environment narrowing the propaganda field of terrorist organization is the primary goal for the security forces. In this sense, providing and maintaining public support is the most necessary ability for security units. Rather than enemy and threat-oriented approach, homeland security oriented approach is essential to ensure public support. In this study, terror assumed as a homeland security issue and assigning the law enforcement forces with military status is analyzed.Keywords: Terrorism, Counter-terrorism, Military Status Law-enforcement.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2193645 An Intelligent System for Knee and Ankle Rehabilitation
Authors: Dimitar Karastoyanov, Vladimir Monov
Abstract:
The paper is concerned with the state examination as well as the problems during the post surgical (orthopedic) rehabilitation of the knee and ankle joint. An observation of the current appliances for a passive rehabilitation devices is presented. The major necessary and basic features of the intelligent rehabilitation devices are considered. An approach for a new intelligent appliance is suggested. The main advantages of the device are: both active as well as passive rehabilitation of the patient based on the human - patient reactions and a real time feedback. The basic components: controller; electrical motor; encoder, force – torque sensor are discussed in details. The main modes of operation of the device are considered.
Keywords: Ankle, knee, rehabilitation, computer control.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2586644 Haemocompatibility of Surface Modified AISI 316L Austenitic Stainless Steel Tested in Artificial Plasma
Authors: W. Walke, J. Przondziono, K. Nowińska
Abstract:
The study comprises evaluation of suitability of passive layer created on the surface of AISI 316L stainless steel for products that are intended to have contact with blood. For that purpose, prior to and after chemical passivation, samples were subject to 7 day exposure in artificial plasma at the temperature of T=37°C. Next, tests of metallic ions infiltration from the surface to the solution were performed. The tests were performed with application of spectrometer JY 2000, by Yobin – Yvon, employing Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES). In order to characterize physical and chemical features of electrochemical processes taking place during exposure of samples to artificial plasma, tests with application of electrochemical impedance spectroscopy were suggested. The tests were performed with application of measuring unit equipped with potentiostat PGSTAT 302n with an attachment for impedance tests FRA2. Measurements were made in the environment simulating human blood at the temperature of T=37°C. Performed tests proved that application of chemical passivation process for AISI 316L stainless steel used for production of goods intended to have contact with blood is well-grounded and useful in order to improve safety of their usage.
Keywords: AISI 316L stainless steel, chemical passivation, artificial plasma, ions infiltration, EIS.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2096643 Effect of Lime on the California Bearing Ratio Behaviour of Fly Ash - mine Overburden Mixes
Authors: B. Behera, M. K. Mishra
Abstract:
Typically thermal power plants are located near to surface coal mines that produce huge amount of fly ash as a waste byproduct. Disposal of fly ash causes significant economic and environmental problems. Now-a-days, research is going on for bulk utilization of fly ash. In order to increase its percentage utilization, an investigation was carried out to evaluate its potential for haul road construction. This paper presents the laboratory California bearing ratio (CBR) tests and evaluates the effect of lime on CBR behavior of fly ash - mine overburden mixes. Tests were performed with different percentages of lime (2%, 3%, 6%, and 9%). The results show that the increase in bearing ratio of fly ash-overburden mixes was achieved by lime treatment. Scanning electron microscopy (SEM) analyses were conducted on 28 days cured specimens. The SEM study showed that the bearing ratio development is related to the microstructural development.Keywords: California bearing ratio, Fly ash, Mine overburden, Lime.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1625642 Photodegradation of Phenol Red in the Presence of ZnO Nanoparticles
Authors: T.K. Tan, P.S. Khiew, W.S. Chiu, S.Radiman, R.Abd-Shukor, N.M. Huang, H.N. Lim
Abstract:
In our recent study, we have used ZnO nanoparticles assisted with UV light irradiation to investigate the photocatalytic degradation of Phenol Red (PR). The ZnO photocatalyst was characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), specific surface area analysis (BET) and UVvisible spectroscopy. X-ray diffractometry result for the ZnO nanoparticles exhibit normal crystalline phase features. All observed peaks can be indexed to the pure hexagonal wurtzite crystal structures, with the space group of P63mc. There are no other impurities in the diffraction peak. In addition, TEM measurement shows that most of the nanoparticles are rod-like and spherical in shape and fairly monodispersed. A significant degradation of the PR was observed when the catalyst was added into the solution even without the UV light exposure. In addition, the photodegradation increases with the photocatalyst loading. The surface area of the ZnO nanomaterials from the BET measurement was 11.9 m2/g. Besides the photocatalyst loading, the effect of some parameters on the photodegradation efficiency such as initial PR concentration and pH were also studied.
Keywords: Nanostructures, phenol red, zinc oxide, heterogeneous photocatalyst.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3163641 Reduction of Content of Lead and Zinc from Wastewater by Using of Metallurgical Waste
Authors: L. Rozumová, J. Seidlerová
Abstract:
The aim of this paper was to study the sorption properties of a blast furnace sludge used as the sorbent. The sorbent was utilized for reduction of content of lead and zinc ions. Sorbent utilized in this work was obtained from metallurgical industry from process of wet gas treatment in iron production. The blast furnace sludge was characterized by X-Ray diffraction, scanning electron microscopy, and XRFS spectroscopy. Sorption experiments were conducted in batch mode. The sorption of metal ions in the sludge was determined by correlation of adsorption isotherm models. The adsorption of lead and zinc ions was best fitted with Langmuir adsorption isotherms. The adsorption capacity of lead and zinc ions was 53.8 mg.g-1 and 10.7 mg.g-1, respectively. The results indicated that blast furnace sludge could be effectively used as secondary material and could be also employed as a low-cost alternative for the removal of heavy metals ions from wastewater.
Keywords: Blast furnace sludge, lead, zinc, sorption.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 903640 The Effect of Screw Parameters on Pullout Strength of Screw Fixation in Cervical Spine
Authors: S. Ritddech, P. Aroonjarattham, K. Aroonjarattham
Abstract:
The pullout strength had an effect on the stability of plate screw fixation when inserted in the cervical spine. Nine different titanium alloy bone screws were used to test the pullout strength through finite element analysis. The result showed that the Moss Miami I can bear the highest pullout force at 1,075 N, which causes the maximum von Mises stress at 858.87 MPa, a value over the yield strength of titanium. The bone screw should have large outer diameter, core diameter and proximal root radius to increase the pullout strength.
Keywords: Pullout strength, Screw parameter, Cervical spine, Finite element analysis.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3155639 Analysis of Polymer Surface Modifications due to Discharges Initiated by Water Droplets under High Electric Fields
Authors: Michael G. Danikas, Ramanujam Sarathi, Pavlos Ramnalis, Stefanos L. Nalmpantis
Abstract:
This paper investigates the influence of various parameters on the behaviour of water droplets on polymeric surfaces under high electric fields. An inclined plane test was carried out to understand the droplet behaviour in strong electric field. Parameters such as water droplet conductivity, droplet volume, polymeric surface roughness and droplet positioning with respect to the electrodes were studied. The flashover voltage is affected by all aforementioned parameters. The droplet positioning is in some cases more vital than the droplet volume. Surface damages were analysed using Scanning Electron Microscopy (SEM) studies and by Energy dispersive X-ray Analysis (EDAX). It is observes that magnitude of discharge have direct influence on amount of surface daKeywords: Water droplet, polymeric surface, hydrophobicity, partial discharges, SEM, EDAX.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2028638 Effects of Coupling Agent on the Properties of Durian Skin Fibre Filled Polypropylene Composite
Authors: Hazleen Anuar, Nur Aimi Mohd Nasir, Yousuf El-Shekeil
Abstract:
Durian skin is a newly explores natural fibre potentially reinforced polyolefin for diverse applications. In this work, investigation on the effect of coupling agent, maleic anhydride polypropylene (MAPP) on the mechanical, morphological, and thermal properties of polypropylene (PP) reinforced with durian skin fibre (DSF) was conducted. The presence of 30 wt% DSF significantly reduced the tensile strength of PP-DSF composite. Interestingly, even though the same trend goes to PP-DSF with the presence of MAPP, the reduction is only about 4% relative to unreinforced PP and 18% higher than PP-DSF without MAPP (untreated composite or UTC). The used of MAPP in treated composite (TC) also increased the tensile modulus, flexural properties and degradation temperature. The enhanced mechanical properties are consistent with good interfacial interaction as evidenced under scanning electron microscopy.Keywords: Durian skin fiber, coupling agent, mechanical properties, thermogravimetry analysis.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2168637 Myotonometry Method for Assessment Muscle Performance
Authors: Rusu Ligia, Cosma Germina, Lica Eliana, Marin Mihnea, Cernăianu Sorina, Copilusi Petre Cristian, Rusu Petre Florinel
Abstract:
The aim of this paper is to present the role of myotonometry in assessment muscle viscoelasticity by measurement of force index (IF) and stiffness (S) at thigh muscle groups. The results are used for improve the muscle training. The method is based on mechanic impulse on the muscle group, that involve a muscle response like acceleration, speed and amplitude curves. From these we have information about elasticity, stiffness beginning from mechanic oscillations of muscle tissue. Using this method offer the possibility for monitoring the muscle capacity for produce mechanic energy, that allows a efficiency of movement with a minimal tissue deformation.Keywords: assessment, infraspinatus syndrome, kinetic therapy, rehabilitation
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2179636 Mechanical Properties of the Palm Fibers Reinforced HDPE Composites
Authors: Daniella R. Mulinari, Araujo J. F. Marina, Gabriella S. Lopes
Abstract:
Natural fibers are used in polymer composites to improve mechanical properties to replace inorganic reinforcing agents produced by non-renewable resources. The present study investigates the tensile and flexural behaviors of palm fibers-high density polyethylene (HDPE) composite as a function of volume fraction. The surface of the fibers was treated by mercerization treatments to improve the wetting behavior of the apolar HDPE. The treatment characterization was obtained by scanning electron microscopy, X-Ray diffraction and infrared spectroscopy. Results evidences that a good adhesion interfacial between fibers-matrix caused an increase strength and modulus flexural as well as tensile strength in the modified fibers/HDPE composites when compared to the pure HDPE and untreated fibers reinforced composites.Keywords: Mechanical properties, palm fibers, polymer composites, surface treatment.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2285635 The Transformative Impact of Telecommunication in Africa: Connecting Nations, Empowering Lives
Authors: I. M. Mungadi, M. S. Argungu
Abstract:
This study delves into the transformative impact of telecommunication in Africa, illuminating its role in connecting nations and empowering lives across the continent. Over recent decades, the rapid expansion of telecommunication infrastructure has become a powerful force, fostering socio-economic growth and development. Beyond the exchange of information, this digital revolution has influenced education, healthcare, commerce, governance, and social interaction. The abstract explores the multifaceted dimensions of telecommunication's influence on Africa, addressing both its positive transformations and the challenges it presents. By examining the dynamic interplay between technological advancements and societal changes, this research contributes to a nuanced understanding of how telecommunication is shaping a more interconnected, informed, and empowered Africa.
Keywords: Transformative, telecommunication, nations, empowering, connecting.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 134634 Effect of Dose Rate of Irradiation on Ultrastructure of Duodenal Mucosa
Authors: L. Labéjof, I. Mororó, P. Galle, G. Barbosa, M.I. Severo, A.H. de Oliveira
Abstract:
Ultrastructure of duodenum mucosa of irradiated rat was studied versus dose rate of irradiation following exposure to gamma rays from 60-Cobalt source. The animals were whole body irradiated at two dose rates (1 Gy.mn-1 and 1 Gy.h-1) and three total doses (1, 2 or 4 Gy) for each dose rate. 24 or 48 h after irradiation, their small intestine was removed and samples of duodenum were processed for observations under a transmission electron microscopy. Samples of duodenum mucosa of control rats were processed in the same way. For the lower dose rate of 1 Gy.h-1, main lesions characteristic of apoptosis were detected within irradiated enterocytes at a total dose of 2 Gy and 24 h after exposure. Necrosis was noted in the samples, 48 h after exposition. For the higher dose rate of 1 Gy.mn-1, fewer changes were detected at all total doses 24 or 48 h irradiation. Thus, it was shown that the appearance of radiationinduced alterations varies not only with increasing total dose and post-irradiation time but especially with decreasing dose rate.Keywords: Dose rate, Radiation Inury, Apoptosis, SmallBowel
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1946633 Transient Currents in a Double Conductor Line above a Conducting Half-Space
Authors: Valentina Koliskina, Inta Volodko
Abstract:
Transient eddy current problem is solved in the present paper by the method of the Laplace transform for the case of a double conductor line located parallel to a conducting half-space. The Fourier sine and cosine integral transforms are used in order to find the Laplace transform of the solution. The inverse Laplace transform of the solution is found in closed form. The integrated electromotive force per unit length of the double conductor line is calculated in the form of an improper integral.Keywords: Transient eddy currents, Laplace transform, double conductor line.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1432632 Use of Carica papaya as a Bio-Sorbent for Removal of Heavy Metals in Wastewater
Authors: W. E. Igwegbe, B. C. Okoro, J. C. Osuagwu
Abstract:
The study assessed the effectiveness of Pawpaw (Carica papaya) wood in reducing the concentrations of heavy metals in wastewater acting as a bio-sorbent. The following heavy metals were considered; Zinc, Cadmium, Lead, Copper, Iron, Selenium, Nickel and Manganese. The physiochemical properties of Carica papaya stem were studied. The experimental sample was sourced from the trunk of a felled matured pawpaw tree. Wastewater for experimental use was prepared by dissolving soil samples collected from a dump site at Owerri, Imo state of Nigeria in water. The concentration of each metal remaining in solution as residual metal after bio-sorption was determined using Atomic absorption Spectrometer. The effects of pH and initial heavy metal concentration were studied in a batch reactor. The results of Spectrometer test showed that there were different functional groups detected in the Carica papaya stem biomass. There was increase in metal removal as the pH increased for all the metals considered except for Nickel and Manganese. Optimum bio-sorption occurred at pH 5.9 with 5g/100ml solution of bio-sorbent. The results of the study showed that the treated wastewater is fit for irrigation purpose based on Canada wastewater quality guideline for the protection of Agricultural standard. This approach thus provides a cost effective and environmentally friendly option for treating wastewater.Keywords: Biomass, bio-sorption, Carica papaya, heavy metal, wastewater.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2818631 Influence of Surface-Treated Coarse Recycled Concrete Aggregate on Compressive Strength of Concrete
Authors: Sallehan Ismail, Mahyuddin Ramli
Abstract:
This paper reports on the influence of surface-treated coarse recycled concrete aggregate (RCA) on developing the compressive strength of concrete. The coarse RCA was initially treated by separately impregnating it in calcium metasilicate (CM) or wollastonite and nanosilica (NS) prepared at various concentrations. The effects of both treatment materials on concrete properties (e.g., slump, density and compressive strength) were evaluated. Scanning electron microscopy (SEM) analysis was performed to examine the microstructure of the resulting concrete. Results show that the effective use of treated coarse RCA significantly enhances the compressive strength of concrete. This result is supported by the SEM analysis, which indicates the formation of a dense interface between the treated coarse RCA and the cement matrix. Coarse RCA impregnated in CM solution results in better concrete strength than NS, and the optimum concentration of CM solution recommended for treated coarse RCA is 10%.
Keywords: Calcium metasilicate, compressive strength, nanosilica, recycled concrete aggregate.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2857630 Multichannel Image Mosaicing of Stem Cells
Authors: Alessandro Bevilacqua, Alessandro Gherardi, Filippo Piccinini
Abstract:
Image mosaicing techniques are usually employed to offer researchers a wider field of view of microscopic image of biological samples. a mosaic is commonly achieved using automated microscopes and often with one “color" channel, whether it refers to natural or fluorescent analysis. In this work we present a method to achieve three subsequent mosaics of the same part of a stem cell culture analyzed in phase contrast and in fluorescence, with a common non-automated inverted microscope. The mosaics obtained are then merged together to mark, in the original contrast phase images, nuclei and cytoplasm of the cells referring to a mosaic of the culture, rather than to single images. The experiments carried out prove the effectiveness of our approach with cultures of cells stained with calcein (green/cytoplasm and nuclei) and hoechst (blue/nuclei) probes.
Keywords: Microscopy, image mosaicing, fluorescence, stem cells.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1484629 Chitosan/Casein Microparticles: Preparation, Characterization and Drug Release Studies
Authors: Selvakumar Dhanasingh, Shunmuga Kumar Nallaperumal
Abstract:
Microparticles carrier systems made from naturally occurring polymers based on chitosan/casein system appears to be a promising carrier for the sustained release of orally and parenteral administered drugs. In the current study we followed a microencapsulation technique based aqueous coacervation method to prepare chitosan/casein microparticles of compositions 1:1, 1:2 and 1:5 incorporated with chloramphenicol. Glutaraldehyde was used as a chemical cross-linking agent. The microparticles were prepared by aerosol method and studied by optical microscopy, infrared spectroscopy, thermo gravimetric analysis, swelling studies and drug release studies at various pH. The percentage swelling of the polymers are found to be in the order pH 4 > pH 10 > pH 7 and the increase in casein composition decrease the swelling percentage. The drug release studies also follow the above order.
Keywords: Chitosan/casein micro particles, chloramphenicol, drug release, microencapsulation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3199628 Suitability of Class F Flyash for Construction Industry: An Indian Scenario
Authors: M. N. Akhtar, J. N. Akhtar
Abstract:
The present study evaluates the properties of class F fly ash as a replacement of natural materials in civil engineering construction industry. The low-lime flash similar to class F is the prime variety generated in India, although it has significantly smaller volumes of high-lime fly ash as compared to class C. The chemical and physical characterization of the sample is carried out with the number of experimental approaches in order to investigate all relevant features present in the samples. For chemical analysis, elementary quantitative results from point analysis and scanning electron microscopy (SEM)/dispersive spectroscopy (EDS) techniques were used to identify the element images of different fractions. The physical properties found very close to the range of common soils. Furthermore, the fly ash-based bricks were prepared by the same sample of class F fly ash and the results of compressive strength similar to that of Standard Clay Brick Grade 1 available in the local market of India.
Keywords: Flyash, class F, class C, chemical, physical, SEM, EDS.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 856627 Effect of Silver Nanoparticles Size Prepared by Photoreduction Method on Optical Absorption Spectra of TiO2/Ag/N719 Dye Composite Films
Authors: C. Photiphitak, P. Rakkwamsuk, P. Muthitamongkol, C. Sae-Kung, C. Thanachayanont
Abstract:
TiO2/Ag composite films were prepared by incorporating Ag in the pores of mesoporous TiO2 films using a photoreduction method. The Ag nanoparticle sizes were in a range of 3.66-38.56 nm. The TiO2/Ag composite films were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscropy (TEM). The TiO2 films and TiO2/Ag composite films were immersed in a 0.3 mM N719 dye solution and characterized by UV-Vis spectrophotometer. The TiO2/Ag/N719 composite film showed that an optimal size of Ag nanoparticles was 19.12 nm and, hence, gave the maximum optical absorption spectra. The improved absorption was due to surface plasmon resonance induced by the Ag nanoparticles to enhance the absorption coefficient of the dye.Keywords: Silver nanoparticle, TiO2/Ag composite films, Optical properties, surface plasmon resonance
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2602626 Feasibility Study of a BLDC Motor with Integrated Drive Circuit
Authors: Jun-Hyuk Choi, Joon Sung Park, Jung-Moo Seo, In-Soung Jung
Abstract:
A brushless DC motor with integrated drive circuit for air management system is presented. Using magnetic equivalent circuit model a basic design of the motor is determined, and specific configurations are inspected thanks to finite element analysis. In order to reduce an unbalanced magnetic force in an axial direction, induced forces between a stator core and a permanent magnet are calculated with respect to the relative positions of them. For the high efficiency, and high power density, BLDC motor and drive are developed. Also vibration mode and eccentricity of a rotor are considered at the rated and maximum rotational speed Through the experimental results, a validity of the simulated one is confirmed.
Keywords: blower, BLDC, inverter
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2215625 The Effect of Dispersed MWCNTs Using SDBS Surfactant on Bacterial Growth
Authors: J.E. Park, G.R. Kim, D.J. Yoon, C.H. Sin, I.S. Park, T.S. Bea, M.H. Lee
Abstract:
Carbon nanotubes (CNTs) are attractive because of their excellent chemical durability mechanical strength and electrical properties. Therefore there is interest in CNTs for not only electrical and mechanical application, but also biological and medical application. In this study, the dispersion power of surfactant-treated multiwalled carbon nanotubes (MWCNTs) and their effect on the antibacterial activity were examined. Surfactant was used sodium dodecyl-benzenesulfonate (SDBS). UV-vis absorbance and transmission electron microscopy(TEM) were used to characterize the dispersion of MWCNTs in the aqueous phase, showing that the surfactant molecules had been adsorbed onto the MWCNTs surface. The surfactant-treated MWCNTs exhibited antimicrobial activities to streptococcus mutans. The optical density growth curves and viable cell number determined by the plating method suggested that the antimicrobial activity of surfactant-treated MWCNTs was both concentration and treatment time-dependent.Keywords: MWCNT, SDBS, surfactant, antibacterial.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3037624 Harvesting of Kinetic Energy of the Raindrops
Authors: K. C. R. Perera, B. G. Sampath, V. P. C. Dassanayake, B. M. Hapuwatte.
Abstract:
This paper presents a methodology to harvest the kinetic energy of the raindrops using piezoelectric devices. In the study 1m×1m PVDF (Polyvinylidene fluoride) piezoelectric membrane, which is fixed by the four edges, is considered for the numerical simulation on deformation of the membrane due to the impact of the raindrops. Then according to the drop size of the rain, the simulation is performed classifying the rainfall types into three categories as light stratiform rain, moderate stratiform rain and heavy thundershower. The impact force of the raindrop is dependent on the terminal velocity of the raindrop, which is a function of raindrop diameter. The results were then analyzed to calculate the harvestable energy from the deformation of the piezoelectric membrane.
Keywords: Raindrop, piezoelectricity, deformation, terminal velocity.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 6595