Search results for: Residual strain
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 746

Search results for: Residual strain

176 Green Function and Eshelby Tensor Based on Mindlin’s 2nd Gradient Model: An Explicit Study of Spherical Inclusion Case

Authors: A. Selmi, A. Bisharat

Abstract:

Using Fourier transform and based on the Mindlin's 2nd gradient model that involves two length scale parameters, the Green's function, the Eshelby tensor, and the Eshelby-like tensor for a spherical inclusion are derived. It is proved that the Eshelby tensor consists of two parts; the classical Eshelby tensor and a gradient part including the length scale parameters which enable the interpretation of the size effect. When the strain gradient is not taken into account, the obtained Green's function and Eshelby tensor reduce to its analogue based on the classical elasticity. The Eshelby tensor in and outside the inclusion, the volume average of the gradient part and the Eshelby-like tensor are explicitly obtained. Unlike the classical Eshelby tensor, the results show that the components of the new Eshelby tensor vary with the position and the inclusion dimensions. It is demonstrated that the contribution of the gradient part should not be neglected.

Keywords: Eshelby tensor, Eshelby-like tensor, Green’s function, Mindlin’s 2nd gradient model, Spherical inclusion.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 725
175 Surface Modification of Titanium Alloy with Laser Treatment

Authors: Nassier A. Nassir, Robert Birch, D. Rico Sierra, S. P. Edwardson, G. Dearden, Zhongwei Guan

Abstract:

The effect of laser surface treatment parameters on the residual strength of titanium alloy has been investigated. The influence of the laser surface treatment on the bonding strength between the titanium and poly-ether-ketone-ketone (PEKK) surfaces was also evaluated and compared to those offered by titanium foils without surface treatment to optimize the laser parameters. Material characterization using an optical microscope was carried out to study the microstructure and to measure the mean roughness value of the titanium surface. The results showed that the surface roughness shows a significant dependency on the laser power parameters in which surface roughness increases with the laser power increment. Moreover, the results of the tensile tests have shown that there is no significant dropping in tensile strength for the treated samples comparing to the virgin ones. In order to optimize the laser parameter as well as the corresponding surface roughness, single-lap shear tests were conducted on pairs of the laser treated titanium stripes. The results showed that the bonding shear strength between titanium alloy and PEKK film increased with the surface roughness increment to a specific limit. After this point, it is interesting to note that there was no significant effect for the laser parameter on the bonding strength. This evidence suggests that it is not necessary to use very high power of laser to treat titanium surface to achieve a good bonding strength between titanium alloy and the PEKK film.

Keywords: Bonding strength, laser surface treatment, PEKK, titanium alloy.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 860
174 Experimental Investigation on Shear Behaviour of Fibre Reinforced Concrete Beams Using Steel Fibres

Authors: G. Beulah Gnana Ananthi, A. Jaffer Sathick, M. Abirami

Abstract:

Fibre reinforced concrete (FRC) has been widely used in industrial pavements and non-structural elements such as pipes, culverts, tunnels, and precast elements. The strengthening effect of fibres in the concrete matrix is achieved primarily due to the bridging effect of fibres at the crack interfaces. The workability of the concrete was reduced on addition of high percentages of steel fibres. The optimum percentage of addition of steel fibres varies with its aspect ratio. For this study, 1% addition of steel has resulted to be the optimum percentage for both Hooked and Crimped Steel Fibres and was added to the beam specimens. The fibres restrain efficiently the cracks and take up residual stresses beyond the cracking. In this sense, diagonal cracks are effectively stitched up by fibres crossing it. The failure of beams within the shear failure range changed from shear to flexure in the presence of sufficient steel fibre quantity. The shear strength is increased with the addition of steel fibres and had exceeded the enhancement obtained with the transverse reinforcement. However, such increase is not directly in proportion with the quantity of fibres used. Considering all the clarification made in the present experimental investigation, it is concluded that 1% of crimped steel fibres with an aspect ratio of 50 is the best type of steel fibres for replacement of transverse stirrups in high strength concrete beams when compared to the steel fibres with hooked ends.

Keywords: Fibre reinforced concrete, steel fibre, shear strength, crack pattern.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 802
173 Preliminary Study of Antimicrobial Activity against Escherichia coli and Probiotic Properties of Lactic Acid Bacteria Isolated from Thailand Fermented Foods

Authors: Phanwipa Pangsri, Yawariyah Weahayee

Abstract:

The lactic acid bacteria (LAB) were isolated from 10 samples of fermented foods (Sa-tor-dong and Bodo) in South locality of Thailand. The 23 isolates of lactic acid bacteria were selected, which were exhibited a clear zone and growth on MRS agar supplemented with CaCO3. All of lactic acid bacteria were tested on morphological and biochemical. The result showed that all isolates were Gram’s positive, non-spore forming but only 10 isolates displayed catalase negative. The 10 isolates including BD1 .1, BD 1.2, BD 2.1, BD2.2, BD 2.3, BD 3.1, BD 4.1, BD 5.2, ST 4.1 and ST 5.2 were selected for inhibition activity determination. Only 2 strains (ST 4.1 and BD 2.3) showed inhibition zone on agar, when using Escherichia coli sp. as target strain. The ST 4.1 showed highest inhibition zone on agar, which was selected for probiotic property testing. The ST4.1 isolate could grow in MRS broth containing a high concentration of sodium chloride 6%, bile salts 7%, pH 4-10 and vary temperature at 15-45°C.

Keywords: Lactic acid bacteria, Probiotic, Antimicrobial.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2582
172 Post Elevated Temperature Effect on the Strength and Microstructure of Thin High Performance Cementitious Composites (THPCC)

Authors: A. Q. Sobia, A. Shyzleen, M. S. Hamidah, I. Azmi, S. F. A. Rafeeqi, S. Ahmad

Abstract:

Reinforced Concrete (RC) structures strengthened with fiber reinforced polymer (FRP) lack in thermal resistance under elevated temperatures in the event of fire. This phenomenon led to the lining of strengthened concrete with thin high performance cementitious composites (THPCC) to protect the substrate against elevated temperature. Elevated temperature effects on THPCC, based on different cementitious materials have been studied in the past but high-alumina cement (HAC)-based THPCC have not been well characterized. This research study will focus on the THPCC based on HAC replaced by 60%, 70%, 80% and 85% of ground granulated blast furnace slag (GGBS). Samples were evaluated by the measurement of their mechanical strength (28 & 56 days of curing) after exposed to 400°C, 600°C and 28°C of room temperature for comparison and corroborated by their microstructure study. Results showed that among all mixtures, the mix containing only HAC showed the highest compressive strength after exposed to 600°C as compared to other mixtures. However, the tensile strength of THPCC made of HAC and 60% GGBS content was comparable to the THPCC with HAC only after exposed to 600°C. Field emission scanning electron microscopy (FESEM) images of THPCC accompanying Energy Dispersive X-ray (EDX) microanalysis revealed that the microstructure deteriorated considerably after exposure to elevated temperatures which led to the decrease in mechanical strength.

Keywords: Ground granulated blast furnace slag, high aluminacement, microstructure at elevated temperature and residual strength.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2379
171 Analysis of Different Resins in Web-to-Flange Joints

Authors: W. F. Ribeiro, J. L. N. Góes

Abstract:

The industrial process adds to engineering wood products features absent in solid wood, with homogeneous structure and reduced defects, improved physical and mechanical properties, bio-deterioration, resistance and better dimensional stability, improving quality and increasing the reliability of structures wood. These features combined with using fast-growing trees, make them environmentally ecological products, ensuring a strong consumer market. The wood I-joists are manufactured by the industrial profiles bonding flange and web, an important aspect of the production of wooden I-beams is the adhesive joint that bonds the web to the flange. Adhesives can effectively transfer and distribute stresses, thereby increasing the strength and stiffness of the composite. The objective of this study is to evaluate different resins in a shear strain specimens with the aim of analyzing the most efficient resin and possibility of using national products, reducing the manufacturing cost. First was conducted a literature review, where established the geometry and materials generally used, then established and analyzed 8 national resins and produced six specimens for each.

Keywords: Engineered wood products, structural resin, wood i-joist.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2316
170 Determining Full Stage Creep Properties from Miniature Specimen Creep Test

Authors: W. Sun, W. Wen, J. Lu, A. A. Becker

Abstract:

In this work, methods for determining creep properties which can be used to represent the full life until failure from miniature specimen creep tests based on analytical solutions are presented. Examples used to demonstrate the application of the methods include a miniature rectangular thin beam specimen creep test under three-point bending and a miniature two-material tensile specimen creep test subjected to a steady load. Mathematical expressions for deflection and creep strain rate of the two specimens were presented for the Kachanov-Rabotnov creep damage model. On this basis, an inverse procedure was developed which has potential applications for deriving the full life creep damage constitutive properties from a very small volume of material, in particular, for various microstructure constitutive  regions, e.g. within heat-affected zones of power plant pipe weldments. Further work on validation and improvement of the method is addressed.

Keywords: Creep damage property, analytical solutions, inverse approach, miniature specimen test.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 782
169 Photomechanical Analysis of Wooden Testing Bodies under Flexural Loadings

Authors: J. Gazzola, I. M. Dal Fabbro, J. Soriano, M. V. G. Silva, S. Rodrigues

Abstract:

Application of wood in rural construction is diffused all around the world since remote times. However, its inclusion in structural design deserves strong support from broad knowledge of material properties. The pertinent literature reveals the application of optical methods in determining the complete field displacement on bodies exhibiting regular as well as irregular surfaces. The use of moiré techniques in experimental mechanics consists in analyzing the patterns generated on the body surface before and after deformation. The objective of this research work is to study the qualitative deformation behavior of wooden testing specimens under specific loading situations. The experiment setup follows the literature description of shadow moiré methods. Results indicate strong anisotropy influence of the generated displacement field. Important qualitative as well as quantitative stress and strain distribution were obtained wooden members which are applicable to rural constructions.

Keywords: Moiré methods, wooden structural material, rural constructions.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1531
168 Total Lipid of Mutant Synechococcus sp. PCC 7002

Authors: Azlin S Azmi, Mus’ab Zainal, Sarina Sulaiman, Azura Amid, Zaki Zainudin

Abstract:

Microalgae lipid is a promising feedstock for biodiesel production. The objective of this work was to study growth factors affecting marine mutant Synechococcus sp. (PCC 7002) for high lipid production. Four growth factors were investigated; nitrogen-phosporus-potassium (NPK) concentration, light intensity, temperature and NaNO3 concentration on mutant strain growth and lipid production were studied. Design Expert v8.0 was used to design the experimental and analyze the data. The experimental design selected was Min-Run Res IV which consists of 12 runs and the response surfaces measured were specific growth rate and lipid concentration. The extraction of lipid was conducted by chloroform/methanol solvents system. Based on the study, mutant Synechococcus sp. PCC 7002 gave the highest specific growth rate of 0.0014 h-1 at 0% NPK, 2500 lux, 40oC and 0% NaNO3. On the other hand, the highest lipid concentration was obtained at 0% NPK, 3500 lux, 30oC and 1% NaNO3.

Keywords: Cyanobacteria, lipid, mutant, marine Synechococcus sp. PCC 7002, specific growth rate.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2671
167 Material Parameter Identification of Modified AbdelKarim-Ohno Model

Authors: M. Cermak, T. Karasek, J. Rojicek

Abstract:

The key role in phenomenological modelling of cyclic plasticity is good understanding of stress-strain behaviour of given material. There are many models describing behaviour of materials using numerous parameters and constants. Combination of individual parameters in those material models significantly determines whether observed and predicted results are in compliance. Parameter identification techniques such as random gradient, genetic algorithm and sensitivity analysis are used for identification of parameters using numerical modelling and simulation. In this paper genetic algorithm and sensitivity analysis are used to study effect of 4 parameters of modified AbdelKarim-Ohno cyclic plasticity model. Results predicted by Finite Element (FE) simulation are compared with experimental data from biaxial ratcheting test with semi-elliptical loading path.

Keywords: Genetic algorithm, sensitivity analysis, inverse approach, finite element method, cyclic plasticity, ratcheting.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2373
166 Nonlinear Seismic Dynamic Response of Continuous Curved Highway Viaducts with Different Bearing Supports

Authors: Rinna Tanaka, Carlos Mendez Galindo, Toshiro Hayashikawa

Abstract:

The results show that the bridge equipped with seismic isolation bearing system shows a high amount of energy dissipation. The purpose of the present study is to analyze the overall performance of continuous curved highway viaducts with different bearing supports, with an emphasis on the effectiveness of seismic isolation based on lead rubber bearing and hedge reaction force bearing system consisted of friction sliding bearing and rubber bearing. The bridge seismic performance has been evaluated on six different cases with six bearing models. The effects of the different arrangement of bearing on the deck superstructure displacements, the seismic damage at the bottom of the piers, movement track at the pier-s top and the total and strain energies absorbed by the structure are evaluated. In conclusion, the results provide sufficient evidence of the effectiveness on the use of seismic isolation on steel curved highway bridges.

Keywords: Curved highway viaducts, non-linear dynamic response, seismic damage.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1637
165 The Biomechanics of Cycling with a Transtibial Prosthesis: A Case Study of a Professional Cyclist

Authors: D. Koutny, D. Palousek, P. Stoklasek, J. Rosicky, L. Tepla, M. Prochazkova, Z. Svoboda, P. Krejci

Abstract:

The article deals with biomechanics of cyclist with unilateral transtibial amputation. Transtibial amputation completely removes ankle and part of muscles of a lower leg which are responsible for production of force during pedaling and causes significant geometric and power asymmetry between the limbs during cycling movement. The primary goal of this work is to assess the effects of length adjustment of the crank on the kinematics and muscle activity of cyclist. The paper presents experimental work, which aims to find a suitable ratio of the length of kinematic components to improve overall athletic performance. The study presents the results of the kinematic analysis of the cycling movement with different crank length realized by tracking camera system together with the results of muscle activity measurements captured by electromyography and measurement of forces in the cranks by strain gauges.

Keywords: Amputation, electromyography, kinematics of cycling, leg asymmetry, motion capture, transtibial prosthesis.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3579
164 Molecular Dynamics Study on Laninamivir Inhibiting Neuraminidases of H5N1 and pH1N1 Influenza a Viruses

Authors: A. Meeprasert, W. Khuntawee, S. Hannongbua, T. Rungrotmongkol

Abstract:

Viral influenza A subtypes H5N1 and pandemic H1N1 (pH1N1) have worldwide emerged and transmitted. The most common anti-influenza drug for treatment of both seasonal and pandemic influenza viruses is oseltamivir that nowadays becomes resistance to influenza neuraminidase. The novel long-acting drug, laninamivir, was discovered for treatment of the patients infected with influenza B and influenza A viruses. In the present study, laninamivir complexed with wild-type strain of both H5N1 and pH1N1 viruses were comparatively determined the structures and drug-target interactions by means of molecular dynamics (MD) simulations. The results show that the hydrogen bonding interactions formed between laninamivir and its binding residues are likely similar for the two systems. Additionally, the presence of intermolecular interactions from laninamivir to the residues in the binding pocket is established through their side chains in accordance with hydrogen bond interactions.

Keywords: Laninamivir, neuraminidase, H5N1, pandemic H1N1, wild-type, MD simulation

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1684
163 A Novel Fuzzy Logic Based Controller to Adjust the Brightness of the Television Screen with Respect to Surrounding Light

Authors: A. V. Sai Balasubramanian, N. Ravi Shankar, S. Subbaraman, R. Rengaraj

Abstract:

One of the major cause of eye strain and other problems caused while watching television is the relative illumination between the screen and its surrounding. This can be overcome by adjusting the brightness of the screen with respect to the surrounding light. A controller based on fuzzy logic is proposed in this paper. The fuzzy controller takes in the intensity of light surrounding the screen and the present brightness of the screen as input. The output of the fuzzy controller is the grid voltage corresponding to the required brightness. This voltage is given to CRT and brightness is controller dynamically. For the given test system data, different de-fuzzifier methods have been implemented and the results are compared. In order to validate the effectiveness of the proposed approach, a fuzzy controller has been designed by obtaining a test data from a real time system. The simulations are performed in MATLAB and are verified with standard system data. The proposed approach can be implemented for real time applications.

Keywords: Fuzzy controller, Grid voltage

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2789
162 Design and Fabrication of Micro-Bubble Oxygenator

Authors: Chiang-Ho Cheng, An-Shik Yang, Hong-Yih Cheng

Abstract:

This paper applies the MEMS technology to design and fabricate a micro-bubble generator by a piezoelectric actuator. Coupled with a nickel nozzle plate, an annular piezoelectric ceramic was utilized as the primary structure of the generator. In operations, the piezoelectric element deforms transversely under an electric field applied across the thickness of the generator. The surface of the nozzle plate can expand or contract because of the induction of radial strain, resulting in the whole structure to bend, and successively transport oxygen micro-bubbles into the blood flow for enhancing the oxygen content in blood. In the tests, a high magnification microscope and a high speed CCD camera were employed to photograph the time evolution of meniscus shape of gaseous bubbles dispensed from the micro-bubble generator for flow visualization. This investigation thus explored the bubble formation process including the influences of inlet gas pressure along with driving voltage and resonance frequency on the formed bubble extent.

Keywords: Micro-bubble, nozzle, oxygenator, piezoelectric.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1355
161 Analytical Prediction of Seismic Response of Steel Frames with Superelastic Shape Memory Alloy

Authors: Mohamed Omar

Abstract:

Superelastic Shape Memory Alloy (SMA) is accepted when it used as connection in steel structures. The seismic behaviour of steel frames with SMA is being assessed in this study. Three eightstorey steel frames with different SMA systems are suggested, the first one of which is braced with diagonal bracing system, the second one is braced with nee bracing system while the last one is which the SMA is used as connection at the plastic hinge regions of beams. Nonlinear time history analyses of steel frames with SMA subjected to two different ground motion records have been performed using Seismostruct software. To evaluate the efficiency of suggested systems, the dynamic responses of the frames were compared. From the comparison results, it can be concluded that using SMA element is an effective way to improve the dynamic response of structures subjected to earthquake excitations. Implementing the SMA braces can lead to a reduction in residual roof displacement. The shape memory alloy is effective in reducing the maximum displacement at the frame top and it provides a large elastic deformation range. SMA connections are very effective in dissipating energy and reducing the total input energy of the whole frame under severe seismic ground motion. Using of the SMA connection system is more effective in controlling the reaction forces at the base frame than other bracing systems. Using SMA as bracing is more effective in reducing the displacements. The efficiency of SMA is dependant on the input wave motions and the construction system as well.

Keywords: Finite element analysis, seismic response, shapesmemory alloy, steel frame, superelasticity

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1845
160 Aerodynamic Bicycle Torque Augmentation with a Wells Turbine in Wheels

Authors: Tsuyoshi Yamazaki, Etsuo Morishita

Abstract:

Cyclists often run through a crosswind and sometimes we experience the adverse pressure. We came to an idea that Wells turbine can be used as power augmentation device in the crosswind something like sails of a yacht. Wells turbine always rotates in the same direction irrespective of the incoming flow direction, and we use it in the small-scale power generation in the ocean where waves create an oscillating flow. We incorporate the turbine to the wheel of a bike. A commercial device integrates strain gauges in the crank of a bike and transmitted force and torque applied to the pedal of the bike as an e-mail to the driver’s mobile phone. We can analyze the unsteady data in a spreadsheet sent from the crank sensor. We run the bike with the crank sensor on the rollers at the exit of a low-speed wind tunnel and analyze the effect of the crosswind to the wheel with a Wells turbine. We also test the aerodynamic characteristics of the turbine separately. Although power gain depends on the flow direction, several Watts increase might be possible by the Wells turbine incorporated to a bike wheel.

Keywords: Aerodynamics, wells turbine, bicycle, wind engineering.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 858
159 Optimization of Growth Conditions for Acidic Protease Production from Rhizopus oligosporus through Solid State Fermentation of Sunflower Meal

Authors: Abdul Rauf, Muhammad Irfan, Muhammad Nadeem, Ishtiaq Ahmed, Hafiz Muhammad Nasir Iqbal

Abstract:

Rhizopus oligosporus was used in the present study for the production of protease enzyme under SSF. Sunflower meal was used as by-product of oil industry incorporated with organic salts was employed for the production of protease enzyme. The main purpose of the present was to study different parameters of protease productivity, its yields and to optimize basal fermentation conditions. The optimal conditions found for protease production using sunflower meal as a substrate in the present study were inoculum size (1%), substrate (Sunflower meal), substrate concentration (20 g), pH (3), cultivation period (72 h), incubation temperature (35oC), substrate to diluent-s ratio (1:2) and tween 81 (1 mL). The maximum production of protease in the presence of cheaper substrate at low concentration and stability at acidic pH, these characteristics make the strain and its enzymes useful in different industry.

Keywords: Acidic protease, Rhizopus oligosporus, Mediaoptimization, Solid state Fermentation

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3004
158 Study on Microbial Pretreatment for Enhancing Enzymatic Hydrolysis of Corncob

Authors: Kessara Seneesrisakul, Erdogan Gulari, Sumaeth Chavadej

Abstract:

The complex structure of lignocellulose leads to great difficulties in converting it to fermentable sugars for the ethanol production. The major hydrolysis impediments are the crystallinity of cellulose and the lignin content. To improve the efficiency of enzymatic hydrolysis, microbial pretreatment of corncob was investigated using two bacterial strains of Bacillus subtilis A 002 and Cellulomonas sp. TISTR 784 (expected to break open the crystalline part of cellulose) and lignin-degrading fungus, Phanerochaete sordida SK7 (expected to remove lignin from lignocellulose). The microbial pretreatment was carried out with each strain under its optimum conditions. The pretreated corncob samples were further hydrolyzed to produce reducing glucose with low amounts of commercial cellulase (25 U·g-1 corncob) from Aspergillus niger. The corncob samples were determined for composition change by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscope (SEM). According to the results, the microbial pretreatment with fungus, P. sordida SK7 was the most effective for enhancing enzymatic hydrolysis, approximately, 40% improvement.

Keywords: Corncob, Enzymatic hydrolysis, Microorganisms, Pretreatment.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2432
157 Bioinformatics and Molecular Biological Characterization of a Hypothetical Protein SAV1226 as a Potential Drug Target for Methicillin/Vancomycin- Staphylococcus aureus Infections

Authors: Nichole Haag, Kimberly Velk, Tyler McCune, Chun Wu

Abstract:

Methicillin/multiple-resistant Staphylococcus aureus (MRSA) are infectious bacteria that are resistant to common antibiotics. A previous in silico study in our group has identified a hypothetical protein SAV1226 as one of the potential drug targets. In this study, we reported the bioinformatics characterization, as well as cloning, expression, purification and kinetic assays of hypothetical protein SAV1226 from methicillin/vancomycin-resistant Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis revealed a low degree of structural similarity with known proteins. Kinetic assays demonstrated that hypothetical protein SAV1226 is neither a domain of an ATP dependent dihydroxyacetone kinase nor of a phosphotransferase system (PTS) dihydroxyacetone kinase, suggesting that the function of hypothetical protein SAV1226 might be misannotated on public databases such as UniProt and InterProScan 5.

Keywords: Dihydroxyacetone kinase, essential genes, Methicillin-resistant Staphylococcus aureus, drug target.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1769
156 Effect of Friction Models on Stress Distribution of Sheet Materials during V-Bending Process

Authors: Maziar Ramezani, Zaidi Mohd Ripin

Abstract:

In a metal forming process, the friction between the material and the tools influences the process by modifying the stress distribution of the workpiece. This frictional behaviour is often taken into account by using a constant coefficient of friction in the finite element simulations of sheet metal forming processes. However, friction coefficient varies in time and space with many parameters. The Stribeck friction model is investigated in this study to predict springback behaviour of AA6061-T4 sheets during V-bending process. The coefficient of friction in Stribeck curve depends on sliding velocity and contact pressure. The plane-strain bending process is simulated in ABAQUS/Standard. We compared the computed punch load-stroke curves and springback related to the constant coefficient of friction with the defined friction model. The results clearly showed that the new friction model provides better agreement between experiments and results of numerical simulations. The influence of friction models on stress distribution in the workpiece is also studied numerically

Keywords: Friction model, Stress distribution, V-bending.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2740
155 Study of Stress Wave Propagation with NHDMOC

Authors: G.Y. Zhang , M.L. Xu, R.Q. Zhang, W.H. Tang

Abstract:

MOC (method of cell) is a new method of investigating wave propagating in material with periodic microstructure, and can reflect the effect of microstructure. Wave propagation in periodically laminated medium consisting of linearly elastic layers can be treated as a special application of this method. In this paper, it was used to simulate the dynamic response of carbon-phenolic to impulsive loading under certain boundary conditions. From the comparison between the results obtained from this method and the exact results based on propagator matrix theory, excellent agreement is achieved. Conclusion can be made that the oscillation periodicity is decided by the thickness of sub-cells. In the end, the NHDMOC method, which permits studying stress wave propagation with one dimensional strain, was applied to study the one-dimensional stress wave propagation. In this paper, the ZWT nonlinear visco-elastic constitutive relationship with 7 parameters, NHDMOC, and corresponding equations were deduced. The equations were verified, comparing the elastic stress wave propagation in SHPB with, respectively, the elastic and the visco-elastic bar. Finally the dispersion and attenuation of stress wave in SHPB with visco-elastic bar was studied.

Keywords: MOC, NHDMOC, visco-elastic, wave propagation

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1930
154 Enhanced Planar Pattern Tracking for an Outdoor Augmented Reality System

Authors: L. Yu, W. K. Li, S. K. Ong, A. Y. C. Nee

Abstract:

In this paper, a scalable augmented reality framework for handheld devices is presented. The presented framework is enabled by using a server-client data communication structure, in which the search for tracking targets among a database of images is performed on the server-side while pixel-wise 3D tracking is performed on the client-side, which, in this case, is a handheld mobile device. Image search on the server-side adopts a residual-enhanced image descriptors representation that gives the framework a scalability property. The tracking algorithm on the client-side is based on a gravity-aligned feature descriptor which takes the advantage of a sensor-equipped mobile device and an optimized intensity-based image alignment approach that ensures the accuracy of 3D tracking. Automatic content streaming is achieved by using a key-frame selection algorithm, client working phase monitoring and standardized rules for content communication between the server and client. The recognition accuracy test performed on a standard dataset shows that the method adopted in the presented framework outperforms the Bag-of-Words (BoW) method that has been used in some of the previous systems. Experimental test conducted on a set of video sequences indicated the real-time performance of the tracking system with a frame rate at 15-30 frames per second. The presented framework is exposed to be functional in practical situations with a demonstration application on a campus walk-around.

Keywords: Augmented reality framework, server-client model, vision-based tracking, image search.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1141
153 Induction of Apoptosis by Newcastle Disease Virus Strains AF220 and V4-UPM in Human Promyelocytic Leukemia (HL60) and Human T-Lymphoblastic Leukemia (CEM-SS) Cells

Authors: Siti Aishah Abu Bakar, Madihah Zawawi, Abdul Manaf Ali, Aini Ideris

Abstract:

Newcastle Disease Virus (NDV), an avian paramyxovirus, is a highly contagious, generalised virus disease of domestic poultry and wild birds characterized by gastro-intestinal, respiratory and nervous signs. In this study, it was shown that NDV strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS cells. Results from MTT cytolytic assay showed that CD50 for NDV AF2240 against HL60 was 130 HAU and NDV V4-UPM against HL60 and CEM-SS were 110.6 and 150.9 HAU respectively. Besides, both strains were found to inhibit the proliferation of cells in a dose dependent manner. The mode of cell death either by apoptosis or necrosis was further analyzed using acridine orange and propidium iodide (AO/PI) staining. Our results showed that both NDV strains induced primarily apoptosis in treated cells at CD50 concentration. In conclusion, both NDV strains caused cytolytic effects primarily via apoptosis in leukemia cells.

Keywords: Apoptosis, Cytolytic, Leukaemia, Newcastle DiseaseVirus

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1975
152 A Development of the Multiple Intelligences Measurement of Elementary Students

Authors: Chaiwat Waree

Abstract:

This research aims at development of the Multiple Intelligences Measurement of Elementary Students. The structural accuracy test and normality establishment are based on the Multiple Intelligences Theory of Gardner. This theory consists of eight aspects namely linguistics, logic and mathematics, visual-spatial relations, body and movement, music, human relations, self-realization/selfunderstanding and nature. The sample used in this research consists of elementary school students (aged between 5-11 years). The size of the sample group was determined by Yamane Table. The group has 2,504 students. Multistage Sampling was used. Basic statistical analysis and construct validity testing were done using confirmatory factor analysis. The research can be summarized as follows; 1. Multiple Intelligences Measurement consisting of 120 items is content-accurate. Internal consistent reliability according to the method of Kuder-Richardson of the whole Multiple Intelligences Measurement equals .91. The difficulty of the measurement test is between .39-.83. Discrimination is between .21-.85. 2). The Multiple Intelligences Measurement has construct validity in a good range, that is 8 components and all 120 test items have statistical significance level at .01. Chi-square value equals 4357.7; p=.00 at the degree of freedom of 244 and Goodness of Fit Index equals 1.00. Adjusted Goodness of Fit Index equals .92. Comparative Fit Index (CFI) equals .68. Root Mean Squared Residual (RMR) equals 0.064 and Root Mean Square Error of Approximation equals 0.82. 3). The normality of the Multiple Intelligences Measurement is categorized into 3 levels. Those with high intelligence are those with percentiles of more than 78. Those with moderate/medium intelligence are those with percentiles between 24 and 77.9. Those with low intelligence are those with percentiles from 23.9 downwards.

Keywords: Multiple Intelligences, Measurement, Elementary Students.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2960
151 Molecular Characterization of Echinococcus granulosus through Amplification of 12S rRNA Gene and Cox1 Gene Fragments from Cattle in Chittagong, Bangladesh

Authors: M. Omer Faruk, A. M. A. M. Zonaed Siddiki, M. Fazal Karim, Md. Masuduzzaman, S. Chowdhury, Md. Shafiqul Islam, M. Alamgir Hossain

Abstract:

The dog tapeworms Echinococcus granulosus develop hydatid cysts in various organs in human and domestic animals worldwide including Bangladesh. The aim of this study was to identify and characterize the genotype of E. granulosus isolated from cattle using 12S rRNA and Cytochrome oxidase 1 (COX 1) genes. A total of 43 hydatid cyst samples were collected from 390 examined cattle samples derived from slaughterhouses. Among them, three cysts were fertile. Genomic DNA was extracted from germinal membrane and/or protoscoleces followed by PCR amplification of mitochondrial 12S rRNA and Cytochrome oxidase 1 gene fragments. The sequence data revealed existence of G1 (64.28%) and possible G3 (21.43%) genotypes for the first time in Bangladesh. The study indicates that common sheep strain G1 is the dominant subtype of E. granulosus in Chittagong region of Bangladesh. This will increase our understanding of the epidemiology of hydatidosis in the southern part of the country and will be useful to plan suitable control measures in the long run.

Keywords: Echinococcus granulosus, molecular characterization, cattle, Bangladesh.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1076
150 sEMG Interface Design for Locomotion Identification

Authors: Rohit Gupta, Ravinder Agarwal

Abstract:

Surface electromyographic (sEMG) signal has the potential to identify the human activities and intention. This potential is further exploited to control the artificial limbs using the sEMG signal from residual limbs of amputees. The paper deals with the development of multichannel cost efficient sEMG signal interface for research application, along with evaluation of proposed class dependent statistical approach of the feature selection method. The sEMG signal acquisition interface was developed using ADS1298 of Texas Instruments, which is a front-end interface integrated circuit for ECG application. Further, the sEMG signal is recorded from two lower limb muscles for three locomotions namely: Plane Walk (PW), Stair Ascending (SA), Stair Descending (SD). A class dependent statistical approach is proposed for feature selection and also its performance is compared with 12 preexisting feature vectors. To make the study more extensive, performance of five different types of classifiers are compared. The outcome of the current piece of work proves the suitability of the proposed feature selection algorithm for locomotion recognition, as compared to other existing feature vectors. The SVM Classifier is found as the outperformed classifier among compared classifiers with an average recognition accuracy of 97.40%. Feature vector selection emerges as the most dominant factor affecting the classification performance as it holds 51.51% of the total variance in classification accuracy. The results demonstrate the potentials of the developed sEMG signal acquisition interface along with the proposed feature selection algorithm.

Keywords: Classifiers, feature selection, locomotion, sEMG.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1494
149 Thermal Stability Boundary of FG Panel under Aerodynamic Load

Authors: Sang-Lae Lee, Ji-Hwan Kim

Abstract:

In this study, it is investigated the stability boundary of Functionally Graded (FG) panel under the heats and supersonic airflows. Material properties are assumed to be temperature dependent, and a simple power law distribution is taken. First-order shear deformation theory (FSDT) of plate is applied to model the panel, and the von-Karman strain- displacement relations are adopted to consider the geometric nonlinearity due to large deformation. Further, the first-order piston theory is used to model the supersonic aerodynamic load acting on a panel and Rayleigh damping coefficient is used to present the structural damping. In order to find a critical value of the speed, linear flutter analysis of FG panels is performed. Numerical results are compared with the previous works, and present results for the temperature dependent material are discussed in detail for stability boundary of the panel with various volume fractions, and aerodynamic pressures.

Keywords: Functionally graded panels, Linear flutter analysis, Supersonic airflows, Temperature dependent material property.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1595
148 Laser Beam Forming of 3 mm Steel Plate and the Evolving Properties

Authors: Stephen Akinlabi, Mukul Shukla, Esther Akinlabi, Marwala Tshilidzi

Abstract:

This paper reports the evolving properties of a 3 mm low carbon steel plate after Laser Beam Forming achieve this objective, the chemical analyse material and the formed components were carried thereafter both were characterized through microhardness profiling microstructural evaluation and tensile testing. showed an increase in the elemental concentration of the component when compared to the as received attributed to the enhancement property of the LBF process Ultimate Tensile Strength (UTS) and the Vickers the formed component shows an increase when compared to the as received material, this was attributed to strain hardening and grain refinement brought about by the LBF process. The microstructure of the as received steel consists of equiaxed ferrit that of the formed component exhibits elongated orming process (LBF). To es of the as received out and compared; profiling, The chemical analyses formed material; this can be process. The microhardness of ferrite and pearlite while grains.

Keywords: Laser beam forming, deformation , deformation, elongated grains

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1894
147 A Simulation Study of E-Glass Reinforced Polyurethane Footbed and Investigation of Parameters Effecting Elastic Behaviour of Footbed Material

Authors: Berkay Ergene, Çağın Bolat

Abstract:

In this study, we mainly focused on a simulation study regarding composite footbed in order to contribute to shoe industry. As a footbed, e-glass fiber reinforced polyurethane was determined since polyurethane based materials are already used for footbed in shoe manufacturing frequently. Flat, elliptical and rectangular grooved shoe soles were modeled and analyzed separately as TPU, 10% glass fiber reinforced, 30% glass fiber reinforced and 50% glass fiber reinforced materials according to their properties under three point bending and compression situations to determine the relationship between model, material type and mechanical behaviours of composite model. ANSYS 14.0 APDL mechanical structural module is utilized in all simulations and analyzed stress and strain distributions for different footbed models and materials. Furthermore, materials constants like young modulus, shear modulus, Poisson ratio and density of the composites were calculated theoretically by using composite mixture rule and interpreted for mechanical aspects.

Keywords: Composite, elastic behaviour, footbed, simulation.

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 752