@article{(Open Science Index):https://publications.waset.org/pdf/4379,
	  title     = {Induction of Apoptosis by Newcastle Disease Virus Strains AF220 and V4-UPM in Human Promyelocytic Leukemia (HL60) and Human T-Lymphoblastic Leukemia (CEM-SS) Cells},
	  author    = {Siti Aishah Abu Bakar and  Madihah Zawawi and  Abdul Manaf Ali and  Aini Ideris},
	  country	= {},
	  institution	= {},
	  abstract     = {Newcastle Disease Virus (NDV), an avian
paramyxovirus, is a highly contagious, generalised virus disease of
domestic poultry and wild birds characterized by gastro-intestinal,
respiratory and nervous signs. In this study, it was shown that NDV
strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic
Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS
cells. Results from MTT cytolytic assay showed that CD50 for NDV
AF2240 against HL60 was 130 HAU and NDV V4-UPM against
HL60 and CEM-SS were 110.6 and 150.9 HAU respectively.
Besides, both strains were found to inhibit the proliferation of cells in
a dose dependent manner. The mode of cell death either by apoptosis
or necrosis was further analyzed using acridine orange and propidium
iodide (AO/PI) staining. Our results showed that both NDV strains
induced primarily apoptosis in treated cells at CD50 concentration. In
conclusion, both NDV strains caused cytolytic effects primarily via
apoptosis in leukemia cells.},
	    journal   = {International Journal of Bioengineering and Life Sciences},
	  volume    = {6},
	  number    = {4},
	  year      = {2012},
	  pages     = {135 - 139},
	  ee        = {https://publications.waset.org/pdf/4379},
	  url   	= {https://publications.waset.org/vol/64},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 64, 2012},
	}