Abdul Rauf and Muhammad Irfan and Muhammad Nadeem and Ishtiaq Ahmed and Hafiz Muhammad Nasir Iqbal
Optimization of Growth Conditions for Acidic Protease Production from Rhizopus oligosporus through Solid State Fermentation of Sunflower Meal
898 - 901
2010
4
12
International Journal of Biotechnology and Bioengineering
https://publications.waset.org/pdf/12762
https://publications.waset.org/vol/48
World Academy of Science, Engineering and Technology
Rhizopus oligosporus was used in the present study
for the production of protease enzyme under SSF. Sunflower meal
was used as byproduct of oil industry incorporated with organic salts
was employed for the production of protease enzyme. The main
purpose of the present was to study different parameters of protease
productivity, its yields and to optimize basal fermentation conditions.
The optimal conditions found for protease production using
sunflower meal as a substrate in the present study were inoculum size
(1), substrate (Sunflower meal), substrate concentration (20 g), pH
(3), cultivation period (72 h), incubation temperature (35oC),
substrate to diluents ratio (12) and tween 81 (1 mL). The maximum
production of protease in the presence of cheaper substrate at low
concentration and stability at acidic pH, these characteristics make
the strain and its enzymes useful in different industry.
Open Science Index 48, 2010