Search results for: Cell Connection Configurations
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1355

Search results for: Cell Connection Configurations

1085 Optimization of PEM Fuel Cell Biphasic Model

Authors: Boubekeur Dokkar, Nasreddine Chennouf, Noureddine Settou, Belkhir Negrou, Abdesslam Benmhidi

Abstract:

The optimal operation of proton exchange membrane fuel cell (PEMFC) requires good water management which is presented under two forms vapor and liquid. Moreover, fuel cells have to reach higher output require integration of some accessories which need electrical power. In order to analyze fuel cells operation and different species transport phenomena a biphasic mathematical model is presented by governing equations set. The numerical solution of these conservation equations is calculated by Matlab program. A multi-criteria optimization with weighting between two opposite objectives is used to determine the compromise solutions between maximum output and minimal stack size. The obtained results are in good agreement with available literature data.

Keywords: Biphasic model, PEM fuel cell, optimization, simulation, specie transport.

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1084 Intrinsic Electromagnetic Fields and Atom-Field Coupling in Living Cells

Authors: Masroor H. S. Bukhari, Z. H. Shah

Abstract:

The possibility of intrinsic electromagnetic fields within living cells and their resonant self-interaction and interaction with ambient electromagnetic fields is suggested on the basis of a theoretical and experimental study. It is reported that intrinsic electromagnetic fields are produced in the form of radio-frequency and infra-red photons within atoms (which may be coupled or uncoupled) in cellular structures, such as the cell cytoskeleton and plasma membrane. A model is presented for the interaction of these photons among themselves or with atoms under a dipole-dipole coupling, induced by single-photon or two-photon processes. This resonance is manifested by conspicuous field amplification and it is argued that it is possible for these resonant photons to undergo tunnelling in the form of evanescent waves to a short range (of a few nanometers to micrometres). This effect, suggested as a resonant photon tunnelling mechanism in this report, may enable these fields to act as intracellular signal communication devices and as bridges between macromolecules or cellular structures in the cell cytoskeleton, organelles or membrane. A brief overview of an experimental technique and a review of some preliminary results are presented, in the detection of these fields produced in living cell membranes under physiological conditions.

Keywords: bioelectromagnetism, cell membrane, evanescentwaves, photon tunnelling, resonance

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1083 Polymorphic Marker Designed from Bioinformatics Sequences Related to Cell Wall Strength for Discrimination of Mangosteen (Garcinia mangostana L.) Clones Resistant to Gamboge Disorder

Authors: E. Mansyah, Sobir, E. Santosa, A. Sisharmini, Sulassih

Abstract:

Gamboge disorder (GD) or fruit damage by the yellow sap is a major problem in mangosteen. Mangosteen plants varied in the level of GD, from very low or non GD to low, moderate and high GD. However it was difficult to differentiate between GD and non GD plants because evaluation of the disorder is strongly influenced by environment. In this study we investigated the usefulness of primer designed from bioinformatics related to cell wall strength, termed as MCWS, to predict GD. Plant materials used were 28 mangosteen plants selected based on percentage of GD categorized as high, moderate, low and very low or non GD. The result showed that the specific DNA fragments were absent in the high GD accessions. The MCWS marker suggests as a novel polymorphic marker for GD in mangosteen as well as a marker for detect variability in mangosteen as apomictic plant.

Keywords: Bioinformatics, cell wall strength, gamboge disorder, mangosteen, polymorphic marker.

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1082 Laboratory Scale Extraction of Sugar Cane using High Electric Field Pulses

Authors: M. N. Eshtiaghi, N. Yoswathana

Abstract:

The aim of this study was to extract sugar from sugarcane using high electric field pulse (HELP) as a non-thermal cell permeabilization method. The result of this study showed that it is possible to permeablize sugar cane cells using HELP at very short times (less than 10 sec.) and at room temperature. Increasing the field strength (from 0.5kV/cm to 2kV/cm) and pulse number (1 to 12) led to increasing the permeabilization of sugar cane cells. The energy consumption during HELP treatment of sugar cane (2.4 kJ/kg) was about 100 times less compared to thermal cell disintegration at 85 <=C (about 271.7 kJ/kg). In addition, it was possible to extract sugar cane at a moderate temperature (45 <=C) using HELP pretreatment. With combination of HELP pretreatment followed by thermal extraction at 75 <=C, extraction resulted in up to 3% more sugar (on the basis of total extractable sugar) compared to samples without HELP pretreatment.

Keywords: Cell permeabilization, High electric field pulses, Non-thermal processing, Sugar cane extraction.

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1081 Automatic Staging and Subtype Determination for Non-Small Cell Lung Carcinoma Using PET Image Texture Analysis

Authors: Seyhan Karaçavuş, Bülent Yılmaz, Ömer Kayaaltı, Semra İçer, Arzu Taşdemir, Oğuzhan Ayyıldız, Kübra Eset, Eser Kaya

Abstract:

In this study, our goal was to perform tumor staging and subtype determination automatically using different texture analysis approaches for a very common cancer type, i.e., non-small cell lung carcinoma (NSCLC). Especially, we introduced a texture analysis approach, called Law’s texture filter, to be used in this context for the first time. The 18F-FDG PET images of 42 patients with NSCLC were evaluated. The number of patients for each tumor stage, i.e., I-II, III or IV, was 14. The patients had ~45% adenocarcinoma (ADC) and ~55% squamous cell carcinoma (SqCCs). MATLAB technical computing language was employed in the extraction of 51 features by using first order statistics (FOS), gray-level co-occurrence matrix (GLCM), gray-level run-length matrix (GLRLM), and Laws’ texture filters. The feature selection method employed was the sequential forward selection (SFS). Selected textural features were used in the automatic classification by k-nearest neighbors (k-NN) and support vector machines (SVM). In the automatic classification of tumor stage, the accuracy was approximately 59.5% with k-NN classifier (k=3) and 69% with SVM (with one versus one paradigm), using 5 features. In the automatic classification of tumor subtype, the accuracy was around 92.7% with SVM one vs. one. Texture analysis of FDG-PET images might be used, in addition to metabolic parameters as an objective tool to assess tumor histopathological characteristics and in automatic classification of tumor stage and subtype.

Keywords: Cancer stage, cancer cell type, non-small cell lung carcinoma, PET, texture analysis.

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1080 Power Allocation in User-Centric Cell-Free Massive MIMO Systems with Limited Fronthaul Capacity

Authors: Siminfar Samakoush Galougah

Abstract:

In this paper, we study two power allocation problems for an uplink user-centric (UC) cell-free massive multiple-input multiple-output (CF-mMIMO) system. Besides, we assume each access point (AP) is connected to a central processing unit (CPU) via fronthaul link with limited capacity. To efficiently use the fronthaul capacity, two strategies for transmitting signals from APs to the CPU are employed; namely: compress-forward-estimate (CFE), estimate-compress-forward (ECF). The capacity of the aforementioned strategies in user-centric CF-mMIMO are drived. Then, we solved the two power allocation problems with minimum Spectral Efficiency (SE) and sum-SE maximization objectives for ECF and CFE strategies.

Keywords: Cell-free massive MIMO, limited capacity fronthaul, spectral efficiency, power allocation problem.

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1079 Determination of an Efficient Differentiation Pathway of Stem Cells Employing Predictory Neural Network Model

Authors: Mughal Yar M, Israr Ul Haq, Bushra Noman

Abstract:

The stem cells have ability to differentiated themselves through mitotic cell division and various range of specialized cell types. Cellular differentiation is a way by which few specialized cell develops into more specialized.This paper studies the fundamental problem of computational schema for an artificial neural network based on chemical, physical and biological variables of state. By doing this type of study system could be model for a viable propagation of various economically important stem cells differentiation. This paper proposes various differentiation outcomes of artificial neural network into variety of potential specialized cells on implementing MATLAB version 2009. A feed-forward back propagation kind of network was created to input vector (five input elements) with single hidden layer and one output unit in output layer. The efficiency of neural network was done by the assessment of results achieved from this study with that of experimental data input and chosen target data. The propose solution for the efficiency of artificial neural network assessed by the comparatative analysis of “Mean Square Error" at zero epochs. There are different variables of data in order to test the targeted results.

Keywords: Computational shcmin, meiosis, mitosis, neuralnetwork, Stem cell SOM;

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1078 Investigating the Influence of Porosity on Thermal and Mechanical Properties of a C/C Composite Using Image Based FE Modelling

Authors: Abdulrahman A. Alghamdi, Paul M. Mummery, Mohammad A. Sheikh

Abstract:

In this paper, 3D image based composite unit cell is constructed from high resolution tomographic images. Through-thickness thermal diffusivity and in-plane Young’s modulus are predicted for the composite unit cell. The accuracy of the image based composite unit cell is tested by comparing its results with the experimental results obtained from laser flash and tensile test. The FE predictions are in close agreement with experimental results. Through-thickness thermal diffusivity and in-plane Young’s modulus of a virgin C/C composite are predicted by replacing the properties of air (porosity) with the properties of carbon matrix. The effect of porosity was found to be more profound on thermal diffusivity than young’s modulus.

Keywords: Porosity, C/C composite, image based FE modelling, CMC.

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1077 Comparison of the Performance of GaInAsSb and GaSb Cells under Different Temperature Blackbody Radiations

Authors: Liangliang Tang, Chang Xu, Xingying Chen

Abstract:

GaInAsSb cells probably show better performance than GaSb cells in low-temperature thermophotovoltaic systems due to lower bandgap; however, few experiments proved this phenomenon so far. In this paper, numerical simulation is used to evaluate GaInAsSb and GaSb cells with similar structures under different radiation temperatures. We found that GaInAsSb cells with n-type emitters show slightly higher output power densities compared with that of GaSb cells with n-type emitters below 1,550 K-blackbody radiation, and the power density of the later cells will suppress the formers above this temperature point. During the temperature range of 1,000~2,000 K, the efficiencies of GaSb cells are about twice of GaInAsSb cells if perfect filters are used to prevent the emission of the non-absorbed long wavelength photons. Several parameters that affect the GaInAsSb cell were analyzed, such as doping profiles, thicknesses of GaInAsSb epitaxial layer and surface recombination velocity. The non-p junctions, i.e., n-type emitters are better for GaInAsSb cell fabrication, which is similar to that of GaSb cells.

Keywords: Thermophotovoltaic cell, GaSb, GaInAsSb, diffused emitters.

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1076 Cytotoxic Effect of Crude Extract of Sea Pen Virgularia gustaviana on HeLa and MDA-MB-231 Cancer Cell Lines

Authors: Sharareh Sharifi, Pargol Ghavam Mostafavi, Ali Mashinchian Moradi, Mohammad Hadi Givianrad, Hassan Niknejad

Abstract:

Marine organisms such as soft coral, sponge, ascidians, and tunicate containing rich source of natural compound have been studied in last decades because of their special chemical compounds with anticancer properties. The aim of this study was to investigate anti-cancer property of ethyl acetate extracted from marine sea pen Virgularia gustaviana found from Persian Gulf coastal (Bandar Abbas). The extraction processes were carried out with ethyl acetate for five days. Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used for qualitative identification of crude extract. The viability of HeLa and MDA-Mb-231 cancer cells was investigated using MTT assay at the concentration of 25, 50, and a 100 µl/ml of ethyl acetate is extracted. The crude extract of Virgularia gustaviana demonstrated ten fractions with different Retention factor (Rf) by TLC and Retention time (Rt) evaluated by HPLC. The crude extract dose-dependently decreased cancer cell viability compared to control group. According to the results, the ethyl acetate extracted from Virgularia gustaviana inhibits the growth of cancer cells, an effect which needs to be further investigated in the future studies.

Keywords: Virgularia gustaviana, Cembrane Diterpene, anti-cancer, HeLa cancer Cell, MDA-Md-231 Cancer cell.

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1075 Work Function Engineering of Functionally Graded ZnO+Ga2O3 Thin Film for Solar Cell and Organic Light Emitting Diodes Applications

Authors: Yong-Taeg Oh, Won Song, Seok-Eui Choi, Bo-Ra Koo, Dong-Chan Shin

Abstract:

ZnO+Ga2O3 functionally graded thin films (FGTFs) were examined for their potential use as Solar cell and organic light emitting diodes (OLEDs). FGTF transparent conducting oxides (TCO) were fabricated by combinatorial RF magnetron sputtering. The composition gradient was controlled up to 10% by changing the plasma power of the two sputter guns. A Ga2O3+ZnO graded region was placed on the top layer of ZnO. The FGTFs showed up to 80% transmittance. Their surface resistances were reduced to < 10% by increasing the Ga2O3: pure ZnO ratio in the TCO. The FGTFs- work functions could be controlled within a range of 0.18 eV. The controlled work function is a very promising technology because it reduces the contact resistance between the anode and Hall transport layers of OLED and solar cell devices.

Keywords: Work Function, TCO, Functionally Graded Thin Films, Resistance, Transmittance.

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1074 Experimental Study on Smart Anchor Head

Authors: Young-Jun You, Ki-Tae Park, Kyu-Wan Lee

Abstract:

Since prestressed concrete members rely on the tensile strength of the prestressing strands to resist loads, loss of even few them could result catastrophic. Therefore, it is important to measure present residual prestress force. Although there are some techniques for obtaining present prestress force, some problems still remain. One method is to install load cell in front of anchor head but this may increase cost. Load cell is a transducer using the elastic material property. Anchor head is also an elastic material and this might result in monitoring monitor present prestress force. Features of fiber optic sensor such as small size, great sensitivity, high durability can assign sensing function to anchor head. This paper presents the concept of smart anchor head which acts as load cell and experiment for the applicability of it. Test results showed the smart anchor head worked good and strong linear relationship between load and response.

Keywords: SHM, prestress force, anchor head, fiber optic sensor

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1073 Effective Cooling of Photovoltaic Solar Cells by Inserting Triangular Ribs: A Numerical Study

Authors: S. Saadi, S. Benissaad, S. Poncet, Y. Kabar

Abstract:

In photovoltaic (PV) cells, most of the absorbed solar radiation cannot be converted into electricity. A large amount of solar radiation is converted to heat, which should be dissipated by any cooling techniques. In the present study, the cooling is achieved by inserting triangular ribs in the duct. A comprehensive two-dimensional thermo-fluid model for the effective cooling of PV cells has been developed. It has been first carefully validated against experimental and numerical results available in the literature. A parametric analysis was then carried out about the influence of the number and size of the ribs, wind speed, solar irradiance and inlet fluid velocity on the average solar cell and outlet air temperatures as well as the thermal and electrical efficiencies of the module. Results indicated that the use of triangular ribbed channels is a very effective cooling technique, which significantly reduces the average temperature of the PV cell, especially when increasing the number of ribs.

Keywords: Effective cooling, numerical modeling, photovoltaic cell, triangular ribs.

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1072 Apoptosis Induced by Low-concentration Ethanol in Hepatocellular Carcinoma Cell Strains and Down-regulated AFP and Survivin Analysis by Proteomic Technology

Authors: Xin Kai, Juan Li, Sexin Huang, Zengliang Bai

Abstract:

Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.

Keywords: Hepatocellular carcinoma, Ethanol, Proteomics, survivin and AFP

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1071 Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81

Authors: M.V.R.K Sarma, Krishna Saharan, Lalit Kumar, Ashwani Gautam, Avhijeet Kapoor, Nishant Srivastava, Vikram Sahai, V.S Bisaria

Abstract:

The fluorescent pseudomonad strain R81 is a root colonizing rhizobacteria which promotes the growth of many plants by various mechanisms. Its broth containing siderophore (ironchelating compound) and 2,4- diacetyl phloroglucinol (DAPG) is used for preparing bioinoculant formulations for agronomical applications. Glycerol was found to be the best carbon source for improved biomass production. Splitting of nitrogen source to NH4Cl and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan at 0.5 % in the medium increased the siderophore production to 850 mg/l. During batch cultivation of the strain in a bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of siderophore and 20 mg/l of DAPG was achieved when glycerol was 15 g/l and C/N ratio was maintained at 12.5. In case of intermittent feeding of fresh medium during fed-batch cultivation, the dry cell mass was increased to 25 g/l with improved production of DAPG to 70 mg/l.

Keywords: Batch cultivation, Fed-batch cultivation, fluorescent pseudomonad, Metabolites

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1070 Fiber Microstructure in Solanum Found in Thailand

Authors: Aree Thongpukdee, Chockpisit Thepsithar, Sujitra Timchookul

Abstract:

The study aimed to investigate characteristics of vegetative tissue for taxonomic purpose and possibly trend of waste application in industry. Stems and branches of 15 species in Solanum found in Thailand were prepared for fiber and examined by light microscopy. Microstructural characteristic data of fiber i.e. fiber length and width, fiber lumen diameter and fiber cell wall thickness were recorded. The longest average fiber cell length (>3.9 mm.) were obtained in S. lycopersicum L. and S. tuberosum L. Fiber cells from S. lycopersicum also revealed the widest average diameter of whole cell and its lumen at >45.5 μm and >29 μm respectively. However fiber cells with thickest wall of > 9.6 μm were belonged to the ornamental tree species, S. wrightii Benth. The results showed that the slenderness ratio, Runkel ratio, and flexibility coefficient, with potentially suitable for feedstock in paper industry fell in 4 exotic species, i.e. Solanumamericanum L., S. lycopersicum, S. seaforthianum Andr., and S. tuberosum L

Keywords: Fiber, microstructure, Solanaceae, Solanum.

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1069 Application of Thermoplastic Microbioreactor to the Single Cell Study of Budding Yeast to Decipher the Effect of 5-Hydroxymethylfurfural on Growth

Authors: Elif Gencturk, Ekin Yurdakul, Ahmet Y. Celik, Senol Mutlu, Kutlu O. Ulgen

Abstract:

Yeast cells are generally used as a model system of eukaryotes due to their complex genetic structure, rapid growth ability in optimum conditions, easy replication and well-defined genetic system properties. Thus, yeast cells increased the knowledge of the principal pathways in humans. During fermentation, carbohydrates (hexoses and pentoses) degrade into some toxic by-products such as 5-hydroxymethylfurfural (5-HMF or HMF) and furfural. HMF influences the ethanol yield, and ethanol productivity; it interferes with microbial growth and is considered as a potent inhibitor of bioethanol production. In this study, yeast single cell behavior under HMF application was monitored by using a continuous flow single phase microfluidic platform. Microfluidic device in operation is fabricated by hot embossing and thermo-compression techniques from cyclo-olefin polymer (COP). COP is biocompatible, transparent and rigid material and it is suitable for observing fluorescence of cells considering its low auto-fluorescence characteristic. The response of yeast cells was recorded through Red Fluorescent Protein (RFP) tagged Nop56 gene product, which is an essential evolutionary-conserved nucleolar protein, and also a member of the box C/D snoRNP complexes. With the application of HMF, yeast cell proliferation continued but HMF slowed down the cell growth, and after HMF treatment the cell proliferation stopped. By the addition of fresh nutrient medium, the yeast cells recovered after 6 hours of HMF exposure. Thus, HMF application suppresses normal functioning of cell cycle but it does not cause cells to die. The monitoring of Nop56 expression phases of the individual cells shed light on the protein and ribosome synthesis cycles along with their link to growth. Further computational study revealed that the mechanisms underlying the inhibitory or inductive effects of HMF on growth are enriched in functional categories of protein degradation, protein processing, DNA repair and multidrug resistance. The present microfluidic device can successfully be used for studying the effects of inhibitory agents on growth by single cell tracking, thus capturing cell to cell variations. By metabolic engineering techniques, engineered strains can be developed, and the metabolic network of the microorganism can thus be manipulated such that chemical overproduction of target metabolite is achieved along with the maximum growth/biomass yield.  

Keywords: COP, HMF, ribosome biogenesis, thermoplastic microbioreactor, yeast.

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1068 Coordinated Multi-Point Scheme Based On Channel State Information in MIMO-OFDM System

Authors: Su-Hyun Jung, Chang-Bin Ha, Hyoung-Kyu Song

Abstract:

Recently, increasing the quality of experience (QoE) is an important issue. Since performance degradation at cell edge extremely reduces the QoE, several techniques are defined at LTE/LTE-A standard to remove inter-cell interference (ICI). However, the conventional techniques have disadvantage because there is a trade-off between resource allocation and reliable communication. The proposed scheme reduces the ICI more efficiently by using channel state information (CSI) smartly. It is shown that the proposed scheme can reduce the ICI with fewer resources.

Keywords: Adaptive beam forming, CoMP, LTE-A, ICI reduction.

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1067 Relations of Progression in Cognitive Decline with Initial EEG Resting-State Functional Network in Mild Cognitive Impairment

Authors: Chia-Feng Lu, Yuh-Jen Wang, Yu-Te Wu, Sui-Hing Yan

Abstract:

This study aimed at investigating whether the functional brain networks constructed using the initial EEG (obtained when patients first visited hospital) can be correlated with the progression of cognitive decline calculated as the changes of mini-mental state examination (MMSE) scores between the latest and initial examinations. We integrated the time–frequency cross mutual information (TFCMI) method to estimate the EEG functional connectivity between cortical regions, and the network analysis based on graph theory to investigate the organization of functional networks in aMCI. Our finding suggested that higher integrated functional network with sufficient connection strengths, dense connection between local regions, and high network efficiency in processing information at the initial stage may result in a better prognosis of the subsequent cognitive functions for aMCI. In conclusion, the functional connectivity can be a useful biomarker to assist in prediction of cognitive declines in aMCI.

Keywords: Cognitive decline, functional connectivity, MCI, MMSE.

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1066 Robot Cell Planning

Authors: Allan Tubaileh, Ibrahim Hammad, Loay Al Kafafi

Abstract:

A new approach to determine the machine layout in flexible manufacturing cell, and to find the feasible robot configuration of the robot to achieve minimum cycle time is presented in this paper. The location of the input/output location and the optimal robot configuration is obtained for all sequences of work tasks of the robot within a specified period of time. A more realistic approach has been presented to model the problem using the robot joint space. The problem is formulated as a nonlinear optimization problem and solved using Sequential Quadratic Programming algorithm.

Keywords: Robotics, Layout.

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1065 Relocation of Plastic Hinge of Interior Beam-Column Connections with Intermediate Bars in Reinforced Concrete and T-Section Steel Inserts in Precast Concrete Frames

Authors: P. Wongmatar, C. Hansapinyo, C. Buachart

Abstract:

Failure of typical seismic frames has been found by plastic hinge occurring on beams section near column faces. On the other hand, the seismic capacity of the frames can be enhanced if the plastic hinges of the beams are shifted away from the column faces. This paper presents detailing of reinforcements in the interior beam– column connections aiming to relocate the plastic hinge of reinforced concrete and precast concrete frames. Four specimens were tested under quasi-static cyclic load including two monolithic specimens and two precast specimens. For one monolithic specimen, typical seismic reinforcement was provided and considered as a reference specimen named M1. The other reinforced concrete frame M2 contained additional intermediate steel in the connection area compared with the specimen M1. For the precast specimens, embedded T-section steels in joint were provided, with and without diagonal bars in the connection area for specimen P1 and P2, respectively. The test results indicated the ductile failure with beam flexural failure in monolithic specimen M1 and the intermediate steel increased strength and improved joint performance of specimen M2. For the precast specimens, cracks generated at the end of the steel inserts. However, slipping of reinforcing steel lapped in top of the beams was seen before yielding of the main bars leading to the brittle failure. The diagonal bars in precast specimens P2 improved the connection stiffness and the energy dissipation capacity.

Keywords: Relocation, Plastic hinge, Intermediate bar, Tsection steel, Precast concrete frame.

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1064 Antimicrobial Properties of Copper in Gram-Negative and Gram-Positive Bacteria

Authors: Travis J. Meyer, Jasodra Ramlall, Phyo Thu, Nidhi Gadura

Abstract:

For centuries humans have used the antimicrobial properties of copper to their advantage. Yet, after all these years the underlying mechanisms of copper mediated cell death in various microbes remain unclear. We had explored the hypothesis that copper mediated increased levels of lipid peroxidation in the membrane fatty acids is responsible for increased killing in Escherichia coli. In this study we show that in both gram positive (Staphylococcus aureus) and gram negative (Pseudomonas aeruginosa) bacteria there is a strong correlation between copper mediated cell death and increased levels of lipid peroxidation. Interestingly, the non-spore forming gram positive bacteria as well as gram negative bacteria show similar patterns of cell death, increased levels of lipid peroxidation, as well as genomic DNA degradation, however there is some difference in loss in membrane integrity upon exposure to copper alloy surface.

Keywords: Antimicrobial, copper, gram positive, gram negative.

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1063 Probabilistic Characteristics of older PR Frames in the Mid-America Earthquake Region

Authors: Do-Hwan Kim, Roberto Leon

Abstract:

Probabilistic characteristics of seismic responses of the Partially Restrained connection rotation (PRCR) and panel zone deformation (PZD) installed in older steel moment frames were investigated in accordance with statistical inference in decision-making process. The 4, 6 and 8 story older steel moment frames with clip angle and T-stub connections were designed and analyzed using 2%/50yrs ground motions in four cities of the Mid-America earthquake region. The probability density function and cumulative distribution function of PRCR and PZD were determined by the goodness-of-fit tests based on probabilistic parameters measured from the results of the nonlinear time-history analyses. The obtained probabilistic parameters and distributions can be used to find out what performance level mainly PR connections and panel zones satisfy and how many PR connections and panel zones experience a serious damage under the Mid-America ground motions.

Keywords: Mid-America earthquake, Panel zone, PR connection, Probabilistic characteristics, seismic performance

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1062 Evaluation for Punching Shear Strength of Slab-Column Connections with Ultra High Performance Fiber-Reinforced Concrete Overlay

Authors: H. S. Youm, S. G. Hong

Abstract:

This paper presents the test results on 5 slab-column connection specimens with Ultra High Performance Fiber-Reinforced Concrete (UHPFRC) overlay including 1 control specimen to investigate retrofitting effect of UHPFRC overlay on the punching shear capacity. The test parameters were the thickness of the UHPFRC overlay and the amount of steel re-bars in it. All specimens failed in punching shear mode with abrupt failure aspect. The test results showed that by adding a thin layer of UHPFRC over the Reinforced Concrete (RC) substrates, considerable increases in global punching shear resistance up to 82% and structural rigidity were achieved. Furthermore, based on the cracking patterns the composite systems appeared to be governed by two failure modes: 1) diagonal shear failure in RC section and 2) debonding failure at the interface.

Keywords: Punching shear strength, retrofit, slab-column connection, UHPFRC, UHPFRC overlay.

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1061 Piezoelectric Bimorph Harvester Based on Different Lead Zirconate Titanate Materials to Enhance Energy Collection

Authors: Irene Perez-Alfaro, Nieves Murillo, Carlos Bernal, Daniel Gil-Hernandez

Abstract:

Nowadays, the increasing applicability of internet of things (IoT) systems has changed the way that the world around is perceived. The massive interconnection of systems by means of sensing, processing and communication, allows multitude of data to be at our fingertips. In this way, countless advances have been made in different fields such as personal care, predictive maintenance in industry, quality control in production processes, security, and in everything imaginable. However, all these electronic systems have in common the need to be electrically powered. In this context, batteries and wires are the most commonly used solutions, but they are not a definitive solution in some applications, because of the attainability, the serviceability, or the performance requirements. Therefore, the need arises to look for other types of solutions based on energy harvesting and long-life electronics. Energy Harvesting can be defined as the action of capturing energy from the environment and store it for an instantaneous use or later use. Among the materials capable of harvesting energy from the environment, such as thermoelectrics, electromagnetics, photovoltaics or triboelectrics, the most suitable is the piezoelectric material. The phenomenon of piezoelectricity is one of the most powerful sources for energy harvesting, ranging from a few micro wats to hundreds of wats, depending on certain factors such as material type, geometry, excitation frequency, mechanical and electrical configurations, among others. In this research work, an exhaustive study is carried out on how different types of piezoelectric materials and electrical configurations influence the maximum power that a bimorph harvester is able to extract from mechanical vibrations. A series of experiments has been carried out in which the manufactured bimorph specimens are excited under fixed inertial vibrational conditions. In addition, in order to evaluate the dependence of the maximum transferred power, different load resistors are tested. In this way, the pure active power that achieves the maximum power transfer can be approximated. In this paper, we present the design of low-cost energy harvesting solutions based on piezoelectric smart materials with tunable frequency. The results obtained show the differences in energy extraction between the PZT materials studied and their electrical configurations. The aim of this work is to gain a better understanding of the behavior of piezoelectric materials, and the design process of bimorph PZT harvesters to optimize environmental energy extraction.

Keywords: Bimorph harvesters, electrical impedance, energy harvesting, piezoelectric, smart material.

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1060 Optimum Radio Capacity Estimation of a Single-Cell Spread Spectrum MIMO System under Rayleigh Fading Conditions

Authors: P. Varzakas

Abstract:

In this paper, the problem of estimating the optimal radio capacity of a single-cell spread spectrum (SS) multiple-inputmultiple- output (MIMO) system operating in a Rayleigh fading environment is examined. The optimisation between the radio capacity and the theoretically achievable average channel capacity (in the sense of information theory) per user of a MIMO single-cell SS system operating in a Rayleigh fading environment is presented. Then, the spectral efficiency is estimated in terms of the achievable average channel capacity per user, during the operation over a broadcast time-varying link, and leads to a simple novel-closed form expression for the optimal radio capacity value based on the maximization of the achieved spectral efficiency. Numerical results are presented to illustrate the proposed analysis.

Keywords: Channel capacity, MIMO systems, Radio capacity, Rayleigh fading, Spectral efficiency.

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1059 Statistical Analysis of Parameters Effects on Maximum Strain and Torsion Angle of FRP Honeycomb Sandwich Panels Subjected to Torsion

Authors: Mehdi Modabberifar, Milad Roodi, Ehsan Souri

Abstract:

In recent years, honeycomb fiber reinforced plastic (FRP) sandwich panels have been increasingly used in various industries. Low weight, low price and high mechanical strength are the benefits of these structures. However, their mechanical properties and behavior have not been fully explored. The objective of this study is to conduct a combined numerical-statistical investigation of honeycomb FRP sandwich beams subject to torsion load. In this paper, the effect of geometric parameters of sandwich panel on maximum shear strain in both face and core and angle of torsion in a honeycomb FRP sandwich structures in torsion is investigated. The effect of Parameters including core thickness, face skin thickness, cell shape, cell size, and cell thickness on mechanical behavior of the structure were numerically investigated. Main effects of factors were considered in this paper and regression equations were derived. Taguchi method was employed as experimental design and an optimum parameter combination for the maximum structure stiffness has been obtained. The results showed that cell size and face skin thickness have the most significant impacts on torsion angle, maximum shear strain in face and core.

Keywords: Finite element, honeycomb FRP sandwich panel, torsion, civil engineering.

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1058 The Impact of the Cell-Free Solution of Lactic Acid Bacteria on Cadaverine Production by Listeria monocytogenes and Staphylococcus aureus in Lysine-Decarboxylase Broth

Authors: Fatih Özogul, Nurten Toy, Yesim Özogul

Abstract:

The influences of cell-free solutions (CFSs) of lactic acid bacteria (LAB) on cadaverine and other biogenic amines production by Listeria monocytogenes and Staphylococcus aureus were investigated in lysine decarboxylase broth (LDB) using HPLC. Cell free solutions were prepared from Lactococcus lactis subsp. lactis, Leuconostoc mesenteroides subsp. cremoris, Pediococcus acidilactici and Streptococcus thermophiles. Two different concentrations that were 50% and 25% CFS and the control without CFSs were prepared. Significant variations on biogenic amine production were observed in the presence of L. monocytogenes and S. aureus (P < 0.05). The function of CFS on biogenic amine production by foodborne pathogens varied depending on strains and specific amine. Cadaverine formation by L. monocytogenes and S. aureus in control were 500.9 and 948.1 mg/L, respectively while the CFSs of LAB induced 4-fold lower cadaverine production by L. monocytogenes and 7-fold lower cadaverine production by S. aureus. The CFSs resulted in strong decreases in cadaverine and putrescine production by L. monocytogenes and S. aureus, although remarkable increases were observed for histamine, spermidine, spermine, serotonin, dopamine, tyramine and agmatine in the presence of LAB in lysine decarboxylase broth.

Keywords: Cell-free solution, lactic acid bacteria, cadaverine, food borne-pathogen.

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1057 Enhanced Imperialist Competitive Algorithm for the Cell Formation Problem Using Sequence Data

Authors: S. H. Borghei, E. Teymourian, M. Mobin, G. M. Komaki, S. Sheikh

Abstract:

Imperialist Competitive Algorithm (ICA) is a recent meta-heuristic method that is inspired by the social evolutions for solving NP-Hard problems. The ICA is a population-based algorithm which has achieved a great performance in comparison to other metaheuristics. This study is about developing enhanced ICA approach to solve the Cell Formation Problem (CFP) using sequence data. In addition to the conventional ICA, an enhanced version of ICA, namely EICA, applies local search techniques to add more intensification aptitude and embed the features of exploration and intensification more successfully. Suitable performance measures are used to compare the proposed algorithms with some other powerful solution approaches in the literature. In the same way, for checking the proficiency of algorithms, forty test problems are presented. Five benchmark problems have sequence data, and other ones are based on 0-1 matrices modified to sequence based problems. Computational results elucidate the efficiency of the EICA in solving CFP problems.

Keywords: Cell formation problem, Group technology, Imperialist competitive algorithm, Sequence data.

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1056 Electrical Performance of a Solid Oxide Fuel Cell Unit with Non-Uniform Inlet Flow and High Fuel Utilization

Authors: Ping Yuan, Mu-Sheng Chiang, Syu-Fang Liu, Shih-Bin Wang, Ming-Jun Kuo

Abstract:

This study investigates the electrical performance of a planar solid oxide fuel cell unit with cross-flow configuration when the fuel utilization gets higher and the fuel inlet flow are non-uniform. A software package in this study solves two-dimensional, simultaneous, partial differential equations of mass, energy, and electro-chemistry, without considering stack direction variation. The results show that the fuel utilization increases with a decrease in the molar flow rate, and the average current density decreases when the molar flow rate drops. In addition, non-uniform Pattern A will induce more severe happening of non-reaction area in the corner of the fuel exit and the air inlet. This non-reaction area deteriorates the average current density and then deteriorates the electrical performance to –7%.

Keywords: Performance, Solid oxide fuel cell, non-uniform, fuelutilization

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