@article{(Open Science Index):https://publications.waset.org/pdf/6767, title = {Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81}, author = {M.V.R.K Sarma and Krishna Saharan and Lalit Kumar and Ashwani Gautam and Avhijeet Kapoor and Nishant Srivastava and Vikram Sahai and V.S Bisaria}, country = {}, institution = {}, abstract = {The fluorescent pseudomonad strain R81 is a root colonizing rhizobacteria which promotes the growth of many plants by various mechanisms. Its broth containing siderophore (ironchelating compound) and 2,4- diacetyl phloroglucinol (DAPG) is used for preparing bioinoculant formulations for agronomical applications. Glycerol was found to be the best carbon source for improved biomass production. Splitting of nitrogen source to NH4Cl and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan at 0.5 % in the medium increased the siderophore production to 850 mg/l. During batch cultivation of the strain in a bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of siderophore and 20 mg/l of DAPG was achieved when glycerol was 15 g/l and C/N ratio was maintained at 12.5. In case of intermittent feeding of fresh medium during fed-batch cultivation, the dry cell mass was increased to 25 g/l with improved production of DAPG to 70 mg/l.}, journal = {International Journal of Biomedical and Biological Engineering}, volume = {4}, number = {5}, year = {2010}, pages = {388 - 392}, ee = {https://publications.waset.org/pdf/6767}, url = {https://publications.waset.org/vol/41}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 41, 2010}, }