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Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81
Abstract:The fluorescent pseudomonad strain R81 is a root colonizing rhizobacteria which promotes the growth of many plants by various mechanisms. Its broth containing siderophore (ironchelating compound) and 2,4- diacetyl phloroglucinol (DAPG) is used for preparing bioinoculant formulations for agronomical applications. Glycerol was found to be the best carbon source for improved biomass production. Splitting of nitrogen source to NH4Cl and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan at 0.5 % in the medium increased the siderophore production to 850 mg/l. During batch cultivation of the strain in a bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of siderophore and 20 mg/l of DAPG was achieved when glycerol was 15 g/l and C/N ratio was maintained at 12.5. In case of intermittent feeding of fresh medium during fed-batch cultivation, the dry cell mass was increased to 25 g/l with improved production of DAPG to 70 mg/l.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1332572Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2024
 A. Sharma, B.N. Johri, A.K. Sharma, B.R. Glick, "Plant growthpromoting bacterium pseudomonas sp. strain GRP3 influences iron acquisition in mung bean (Vigna radiata L. Wilzeck)," Soil. Biol. Biochem, vol. 35, pp 887-894, Mar. 2003.
 A.R. Podile, and G.K. Kishore, "Plant Growth Promoting Rhizobacteria," in Plant-associated bacteria, 1st ed. S.S. Gnanamanickam, Ed, Netherlands, Springer, 2006, pp 195-230.
 J. Leong, J, "Siderophores: their biochemistry and possible role in the biocontrol of plant pathogen," Ann. Rev. Phytopathol, vol. 24, pp 187- 209, 1986.
 C. Voisard, C. Keel, D. Hass, and G. Defago, "Cyanide production by Pseudomonas fluorescens suppress helps black root rot of tobacco under gnotobiotic conditions," EMBO J, vol. 8, pp 351-358. 1989.
 R. Gaur, S. Noam, S. Kawaljeet, B.N. Johri, P. Rossi, and M. Aragno, "Diacetylphloroglucinol producing pseudomonads do not influence AM fungi in wheat rhizosphere," Curr. Sci, vol. 86, pp 453-457. Feb. 2004.
 D.J. O-Sullivan, and F. O-Gara, "Traits of fluorescent pseudomonas sp. involved in suppression of plant root pathogens," Microbiol. Rev, vol. 56, pp 662-676, Dec.1992.
 D. Roesti, R. Gaur, B.N. Johri, G. Imfeld, S. Sharma, K. Kwaljeet, and M. Aragno, "Plant growth stage, fertilizer management and bioinoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields," Soil. Biol. Biochem, vol. 38, pp 1111-1120, Jan. 2006.
 M. Aragno, and H.G. Schlegel, "The mesophilic hydrogen-oxidizing (Knallgas) bacteria," in The Prokaryotes, A. Balows, H.G. Tr├╝per, M. Dworkin, W. Harder, and K.H. Schleifer KH, Eds. Berlin Heidelberg New York: Springer Verlag, 1991, pp. 344-384.
 J.M. Meyer, and M.A. Abdallah, "The florescent pigment of pseudomonas fluorescens biosynthesis, purification and physicalchemical properties," J Gen Microbiol, vol. 107, pp 319-328, April 1978.
 S.H. Bok, and A.L. Demain, "An improved colorimetric assay for polyols," Anal. Biochem, vol. 81, pp.18-20, March 1977.
 S.H. Hua, T. Yoshida, Y. Meng, T. Kabashima, K. Ito, Y. Nishiya, Y. Kawamura, and T. Yoshimoto, "Purification and characterization of thermostable glycerol kinase from Thermus flavus," J. Ferment. Bioeng, vol. 83, pp 328-332, Jan. 1997.
 D. Rachid, and B. Ahmed, "Effect of iron and growth inhibitors on siderophores production by Pseudomonas fluorescens" African J. Biotechnol, vol. 4, pp 697-702, July 2005.
 E. Mar├¡a, and D├¡az de Villegas "Biotechnological production of siderophores," in Microbial Siderophores, Series: Soil Biology, Vol. 12, A. Varma, and S. Chincholkar, Eds, Berlin, Heidelberg: Springer Verlag, 2007, pp. 219-231.
 T. Yamane, and S. Shimizu, "Fed-batch techniques in microbial processes," Adv. Biochem. Eng, vol. 30, pp 147-194. 1984.
 L. Yee, and H.W. Blanch, "Recombinant trypsin production in high cell density fed-batch cultures in Escherichia coli," Biotechnol. Bioeng, vol. 41, pp 781-790, April 1993.