Cytotoxic Effect of Crude Extract of Sea Pen Virgularia gustaviana on HeLa and MDA-MB-231 Cancer Cell Lines
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Cytotoxic Effect of Crude Extract of Sea Pen Virgularia gustaviana on HeLa and MDA-MB-231 Cancer Cell Lines

Authors: Sharareh Sharifi, Pargol Ghavam Mostafavi, Ali Mashinchian Moradi, Mohammad Hadi Givianrad, Hassan Niknejad


Marine organisms such as soft coral, sponge, ascidians, and tunicate containing rich source of natural compound have been studied in last decades because of their special chemical compounds with anticancer properties. The aim of this study was to investigate anti-cancer property of ethyl acetate extracted from marine sea pen Virgularia gustaviana found from Persian Gulf coastal (Bandar Abbas). The extraction processes were carried out with ethyl acetate for five days. Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used for qualitative identification of crude extract. The viability of HeLa and MDA-Mb-231 cancer cells was investigated using MTT assay at the concentration of 25, 50, and a 100 µl/ml of ethyl acetate is extracted. The crude extract of Virgularia gustaviana demonstrated ten fractions with different Retention factor (Rf) by TLC and Retention time (Rt) evaluated by HPLC. The crude extract dose-dependently decreased cancer cell viability compared to control group. According to the results, the ethyl acetate extracted from Virgularia gustaviana inhibits the growth of cancer cells, an effect which needs to be further investigated in the future studies.

Keywords: Virgularia gustaviana, Cembrane Diterpene, anti-cancer, HeLa cancer Cell, MDA-Md-231 Cancer cell.

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[1] L. C. Hu, J. H. Su, M. Y. Chiang, M. C. Lu, T. L. Hwang, Y. H. Chen, W. P. Hu, N. C. Lin, W. H. Wang, L. S. Fang, Y. H. Kuo, P. J. Sung, Flexibilins A-C, new cembrane-type diterpenoids from the Formosan soft coral, Sinularia flexibilis, Marine drugs, 11 (2013) 1999-2012.
[2] J. Rocha, L. Peixe, N. C. Gomes, R. Calado, Cnidarians as a source of new marine bioactive compounds--an overview of the last decade and future steps for bioprospecting, Marine drugs, 9 (2011) 1860-1886.
[3] R. Montaser, H. Luesch, Marine natural products: a new wave of drugs? Future medicinal chemistry, 3 (2011) 1475-1489.
[4] J. W. Blunt, B. R. Copp, R. A. Keyzers, M. H. G. Munro, M. R. Prinsep, Marine natural products, Natural Product Reports, 33 (2016) 382-431.
[5] T. E. Adrian, Novel marine-derived anti-cancer agents, Current pharmaceutical design, 13 (2007) 3417-3426.
[6] M. Bayet-Robert, S. Lim, C. Barthomeuf, D. Morvan, Biochemical disorders induced by cytotoxic marine natural products in breast cancer cells as revealed by proton NMR spectroscopy-based metabolomics, Biochemical pharmacology, 80 (2010) 1170-1179.
[7] S. S. Al-Lihaibi, W. M. Alarif, A. Abdel-Lateff, S.-E.N. Ayyad, A. B. Abdel-Naim, F. F. El-Senduny, F. A. Badria, Three new cembranoid-type diterpenes from Red Sea soft coral Sarcophyton glaucum: Isolation and antiproliferative activity against HepG2 cells, European journal of medicinal chemistry, 81 (2014) 314-322.
[8] R. Kazlauskas, P. Murphy, R. Wells, P. Schonholzer, J. Coll, Cembranoid constituents from an Australian collection of the soft coral Sinularia flexibilis, Australian Journal of Chemistry, 31 (1978) 1817-1824.
[9] S. Bilasy Sel, S. I. Khalifa, S. M. Saleh, S. H. Abou El-Ela, HPLC method for the quantitative determination of sarcophine, a source of cembranoids with cancer chemopreventive activity, Journal of pharmaceutical and biomedical analysis, 46 (2008) 784-787.
[10] H. Niknejad, G. Yazdanpanah, Anticancer effects of human amniotic membrane and its epithelial cells, Medical hypotheses, 82 (2014) 488-489.
[11] J. M. Arif, S. S. Sawant, K. A. El Sayed, M. Kunhi, M. P. Subramanian, Y. M. Siddiqui, D. T. Youssef, K. Al-Hussain, M. N. Al-Ahdal, F. Al-Khodairy, Antiproliferative potential of sarcophine and its semisynthetic sulfur-containing derivatives against human mammary carcinoma cell lines, Journal of Natural Medicines, 61 (2007) 154-158.
[12] H. Niknejad, M. Khayat-Khoei, H. Peirovi, H. Abolghasemi, Human amniotic epithelial cells induce apoptosis of cancer cells: a new anti-tumor therapeutic strategy, Cytotherapy, 16 (2014) 33-40.
[13] H. Niknejad, G. Yazdanpanah, M. Mirmasoumi, H. Abolghasemi, H. Peirovi, A. Ahmadiani, Inhibition of HSP90 could be possible mechanism for anti-cancer property of amniotic membrane, Medical hypotheses, 81 (2013) 862-865.
[14] F. Reyes, A. Arda, R. Martin, R. Fernandez, A. Rueda, D. Montalvo, C. Gomez, C. Jimenez, J. Rodriguez, J.M. Sanchez-Puelles, New cytotoxic cembranes from the sea pen Gyrophyllum sibogae, Journal of natural products, 67 (2004) 1190-1192.
[15] I. Bhatnagar, S.K. Kim, Marine antitumor drugs: status, shortfalls and strategies, Marine drugs, 8 (2010) 2702-2720.
[16] M. Zidane, P. Pondaven, C. Roussakis, M. T. More, Effects in vitro of pachymatismin, a glycoprotein from the marine sponge Pachymatisma johnstonii, on a non-small-cell bronchopulmonary carcinoma line (NSCLC-N6), Anticancer Res, 16 (1996) 2805-2812.
[17] R. E. Curiel, C. S. Garcia, L. Farooq, M. F. Aguero, I. Espinoza-Delgado, Bryostatin-1 and IL-2 synergize to induce IFN-gamma expression in human peripheral blood T cells: implications for cancer immunotherapy, Journal of immunology, 167 (2001) 4828-4837.
[18] S. Ankisetty, S. I. Khan, B. Avula, D. Gochfeld, I. A. Khan, M. Slattery, Chlorinated didemnins from the tunicate Trididemnum solidum, Marine drugs, 11 (2013) 4478-4486.
[19] C.-H. Liang, G.-H. Wang, C.-C. Liaw, M.-F. Lee, S.-H. Wang, D.-L. Cheng, T.-H. Chou, Extracts from Cladiella australis, Clavularia viridis and Klyxum simplex (soft corals) are capable of inhibiting the growth of human oral squamous cell carcinoma cells, Marine drugs, 6 (2008) 595-606.
[20] K. Sreejamole, C. Radhakrishnan, Antioxidant and cytotoxic activities of ethyl acetate extract of the Indian green mussel Perna viridis, Asian Journal of Pharmaceutical and Clinical Research, 6 (2013) 197-201.
[21] G. H. Wang, T. H. Chou, R. J. Lin, J. H. Sheu, S. H. Wang, C. H. Liang, Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells, Journal of toxicology, 2009 (2009) 634868.
[22] N. T. Ngoc, N. P. Thao, T. T. Huong, D. C. Thung, N. V. Thanh, N. H. Nam, N. X. Cuong, Y. H. Kim, P. V. Kiem, C. V. Minh, Polyhydroxylated sterols from the soft coral sarcophyton pauciplicatum, Vietnam Journal of Chemistry, 53 (2015) 18-22.
[23] S. Sharifi, Fatty Acid Extracts of Sea Pen (Virgularia gustaviana) and Their Potential Applications as Antibacterial, Antifungal, and Anti-Inflammatory Agents, Biotechnology and Bioengineering, 212 (2016) 10005579.
[24] S. Sharifi, S. Safaeian, Anti-Inflammatory Effect Of Lipid Extract Of Sea Pen (Virgularia Gustaviana) In Mice, Asian Journal of Pharmaceutical and Clinical Research, 8 (2015).