%0 Journal Article
	%A M.V.R.K Sarma and  Krishna Saharan and  Lalit Kumar and  Ashwani Gautam and  Avhijeet Kapoor and  Nishant Srivastava and  Vikram Sahai and  V.S Bisaria
	%D 2010
	%J International Journal of Biomedical and Biological Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 41, 2010
	%T Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81
	%U https://publications.waset.org/pdf/6767
	%V 41
	%X The fluorescent pseudomonad strain R81 is a root
colonizing rhizobacteria which promotes the growth of many plants
by various mechanisms. Its broth containing siderophore (ironchelating
compound) and 2,4- diacetyl phloroglucinol (DAPG) is
used for preparing bioinoculant formulations for agronomical
applications. Glycerol was found to be the best carbon source for
improved biomass production. Splitting of nitrogen source to NH4Cl
and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan
at 0.5 % in the medium increased the siderophore
production to 850 mg/l. During batch cultivation of the strain in a
bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of
siderophore and 20 mg/l of DAPG was achieved when glycerol was
15 g/l and C/N ratio was maintained at 12.5. In case of intermittent
feeding of fresh medium during fed-batch cultivation, the dry cell
mass was increased to 25 g/l with improved production of DAPG to
70 mg/l.
	%P 388 - 392