Polymorphic Marker Designed from Bioinformatics Sequences Related to Cell Wall Strength for Discrimination of Mangosteen (Garcinia mangostana L.) Clones Resistant to Gamboge Disorder

Authors: E. Mansyah, Sobir, E. Santosa, A. Sisharmini, Sulassih

Abstract:

Gamboge disorder (GD) or fruit damage by the yellow sap is a major problem in mangosteen. Mangosteen plants varied in the level of GD, from very low or non GD to low, moderate and high GD. However it was difficult to differentiate between GD and non GD plants because evaluation of the disorder is strongly influenced by environment. In this study we investigated the usefulness of primer designed from bioinformatics related to cell wall strength, termed as MCWS, to predict GD. Plant materials used were 28 mangosteen plants selected based on percentage of GD categorized as high, moderate, low and very low or non GD. The result showed that the specific DNA fragments were absent in the high GD accessions. The MCWS marker suggests as a novel polymorphic marker for GD in mangosteen as well as a marker for detect variability in mangosteen as apomictic plant.

Keywords: Bioinformatics, cell wall strength, gamboge disorder, mangosteen, polymorphic marker.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1070619

References:

[1] Sabar. " Kebijakan Departemen Perdagangan di bidang ekspor buahbuahan," Temu Pelaku Agribisnis Mangga dan Manggis, Bandung, 29-30 November 2005.
[2] O. Yaacob, H.D., Tindall. "Mangosteen Cultivation. FAO Plant Protecion Paper 129. Food and Agriculture Organization of the United Nations. Rome. 1995
[3] Dorly, S. Tjitrosemito, R. Poerwanto. Juliarni. "Secretory Duct Structure and Phytochemistry Compounds of Yellow Latex in Mangosteen Fruit," HAYATI Journal of Biosciences, vol. 15, no. 3, pp. 99-104, Sept.2008
[4] N. C. Carpita, "Tensile Strength of Cell Walls of Living Cells", Plant Physiol, vol. 79, pp. 485-488. 1985.
[5] E. Mansyah, M.A.S Jawal , I. Muas Jumjunidang, T. Purnaman, Riska," Hasil-hasil penelitian tentang getah kuning pada buah manggis (gamboge disorder) di Balitbu Tropika". Seminar Nasional Program dan Pengembangan buah Nusantara. Solok, 10 November 2010, pp. 190-203
[6] J. E K. Cox , "Garcinia mangostana L. - Mangosteen,". in Propagation of Tropical fruit Trees, Common Wealth Bureau. R.J. Gardner, Ed. Farn Harn Royal. England, 1976, pp. 361- 367.
[7] Y. Li, Q. Qian, Y. Zhou, M. Yan M, L. Sun, M. Zhang, Z. Fu, Y. Wang, B. Han, X. Pang, M. Chen, and J. Li, "BRITTLE CULM1, Which Encodes a COBRA-Like Protein, Affects the Mechanical Properties of Rice Plant". The Plant Cell, vol. 15, pp. 2020-2031. 2003
[8] E. Mansyah, A. Baihaki, R. Setiamihardja, J.S. Darsa, Sobir, R. Poerwanto. "Analisis variabilitas genetik manggis (Garcinia mangostana L.) di Jawa dan Sumatera Barat menggunakan teknik RAPD". Zuriat, vol 4, no. 1, pp. 35-44. 2003b.
[9] S. Sinaga, Sobir, R. Poerwanto, H. Aswidinnoor, D. Duryadi, "Genetic variability analysis on apomictic mangosteen (Garcinia mangostana) in Indonesian and its close related species by using RAPD markers". Floribunda, vol. 3, no. 4, pp. 77 - 83. 2007
[10] C. M. Ramage, L. Sando, C.P. Peace, B.J. Caroll , R.A. Drew, " Genetic diversity revealed in the apomictic fruit species Garcinia mangostana L. (mangosteen)". Euphytica. vol. 136, no. 1, pp. 1-10, 2004.
[11] Sobir, S. Sinaga, R. Poerwanto, Rismitasari, R. Lukman, "Comparison analysis of genetic diversity of Indonesian mangosteen (Garcinia mangostana L.) and related species by using isoenzym and AFLP markers". Jurnal Biodiversitas, vol. 10, no. 2, pp. 163-167. 2009.
[12] E.Mansyah, Sobir, E. Santosa, R. Poerwanto, "Assessment of inter simple sequence repeat (ISSR) technique in mangosteen (Garcinia mangostana L.) grown in different Sumatra region", Journal of Horticulture and Forestry, vol. 2, no. 6, pp. 127-134. 2010.
[13] E. Mansyah, I. Muas, M. A. S. Jawal, Sobir, " Morphological variability of apomictic mangosteen (Garcinia mangostana L.) in Indonesia: morphological evidence of natural populations from Sumatera region". SABRAO J. Breeding Genet. vol. 4, no. 2, pp. 1-8. 2010.
[14] M. A. Innis, D. H. Gelfand, "Optimization of PCRs". In PCR Protocols, Innis, Gelfand, Sninsky and White, Eds. Academic Press, New York. 1990, pp. 3-12.
[15] J. J. Doyle, J. L. Doyle. "Isolation of plant DNA from fresh tissues". Focus, vol. 12, pp. 13-15. 1987.
[16] R. Zhong, D. H. Burk, W. H. Morrison, Z. H. Ye,. "A Kinesin-Like Protein Is Essential for Oriented Deposition of Cellulose Microfibrils and Cell Wall Strength". The Plant Cell, Vol. 14, 3101-3117. 2002
[17] A. Chan, D. Puiu, A. Melake, J. Orvis, Q. Zhao, J. Wortman, T. Utterback, M. J. Rosovitz, J.M. Inman, P. Amedeo, S. Schobel, K. Galinsky, C. Fraser, J. Ravel, P. Rabinowicz, "NCBI Reference Sequence: XM_002510131.1 Ricinus communis Kinesin heavy chain, putative, mRNA" Submitted (22-OCT-2008) J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD 20850, USA
[18] A.J. Richards, Plant Breeding Systems. Second Edition. Departemen of Agricultural and Environtmental Science University of Newcastle Upon Tyne. Chapman and Hall. London. 529 pp. 1997.
[19] A. Kokubo, S. Kuraishi, N. Sakurai, "Culm Strength of Barley: Correlation Among Maximum Bending Stress, Cell Wall Dimensions, and Cellulose Content". Plant Physiol.vol. 91, pp. 876-882. 1989.
[20] F. Nicol, H. Ho¨fte, "Plant cell expansion: scaling the wall". Current Opinion in Plant Biology, vol. 1, pp. 12-17. 1998.