Search results for: induced breeding
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 607

Search results for: induced breeding

517 Modelling the Role of Prophylaxis in Malaria Prevention

Authors: Farai Nyabadza

Abstract:

Malaria is by far the world-s most persistent tropical parasitic disease and is endemic to tropical areas where the climatic and weather conditions allow continuous breeding of the mosquitoes that spread malaria. A mathematical model for the transmission of malaria with prophylaxis prevention is analyzed. The stability analysis of the equilibria is presented with the aim of finding threshold conditions under which malaria clears or persists in the human population. Our results suggest that eradication of mosquitoes and prophylaxis prevention can significantly reduce the malaria burden on the human population.

Keywords: Prophylaxis prevention, basic reproductive number, stability.

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516 Induction of alpha-Amylase in Wheat Grain Cultivars as an Indicator of Resistance to Pre-harvest Sprouting

Authors: Aidar A. Khakimzhanov, Vladimir A. Kuzovlev, Nurgul S. Mamytova, Dinara A. Shansharova, Oleg V. Fursov

Abstract:

The influence of humidity and low temperature on the α- amylase activity and isoenzyme composition of grains of different wheat varieties have been studied. The identified samples of varieties have significant difference in the level of enzyme induction under the impact of high humidity and low temperature. It is proposed to use this methodological approach for testing genotypes and wheat breeding lines for resistance to pre-harvest sprouting (PHS).

Keywords: α-Amylase, isoenzymes, wheat, pre-harvest sprouting.

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515 Tidal Flow Patterns Near A Coastal Headland

Authors: Fu E. Tang, Daoyi Chen

Abstract:

Experimental investigations were carried out in the Manchester Tidal flow Facility (MTF) to study the flow patterns in the region around and adjacent to a hypothetical headland in tidal (oscillatory) ambient flow. The Planar laser-induced fluorescence (PLIF) technique was used for visualization, with fluorescent dye released at specific points around the headland perimeter and in its adjacent recirculation zone. The flow patterns can be generalized into the acceleration, stable flow and deceleration stages for each halfcycle, with small variations according to location, which are more distinct for low Keulegan-Carpenter number (KC) cases. Flow patterns in the mixing region are unstable and complex, especially in the recirculation zone. The flow patterns are in agreement with previous visualizations, and support previous results in steady ambient flow. It is suggested that the headland lee could be a viable location for siting of pollutant outfalls.

Keywords: Planar laser-induced Fluorescence, recirculation zone, tidal flow, wake flows

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514 Influence of Calcium Intake Level to Osteoporptic Vertebral bone and Degenerated Disc in Biomechanical Study

Authors: Dae Gon Woo, Ji Hyung Park, Chi Hoon Kim, Tae Woo Lee, Beob Yi Lee, Han Sung Kim

Abstract:

The aim of the present study is to analyze the generation of osteoporotic vertebral bone induced by lack of calcium during growth period and analyze its effects for disc degeneration, based on biomechanical and histomorphometrical study. Mechanical and histomorphological characteristics of lumbar vertebral bones and discs of rats with calcium free diet (CFD) were detected and tracked by using high resolution in-vivo micro-computed tomography (in-vivo micro-CT), finite element (FE) and histological analysis. Twenty female Sprague-Dawley rats (6 weeks old, approximate weight 170g) were randomly divided into two groups (CFD group: 10, NOR group: 10). The CFD group was maintained on a refmed calcium-controlled semisynthetic diet without added calcium, to induce osteoporosis. All lumbar (L 1-L6) were scanned by using in vivo micro-CT with 35i.un resolution at 0, 4, 8 weeks to track the effects of CFD on the generation of osteoporosis. The fmdings of the present study indicated that calcium insufficiency was the main factor in the generation of osteoporosis and it induced lumbar vertebral disc degeneration. This study is a valuable experiment to firstly evaluate osteoporotic vertebral bone and disc degeneration induced by lack of calcium during growth period from a biomechanical and histomorphometrical point of view.

Keywords: Calcium free diet, Disc degeneration, Osteoporosis, in-vivo micro-CT, Finite element analysis, Histology.

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513 Bridge Analysis Structure under Human Induced Dynamic Load

Authors: O. Kratochvíl, J. Križan

Abstract:

The paper deals with the analysis of the dynamic response of footbridges under human - induced dynamic loads. This is a frequently occurring and often dominant load for footbridges as it stems from the very purpose of a footbridge - to convey pedestrian. Due to the emergence of new materials and advanced engineering technology, slender footbridges are increasingly becoming popular to satisfy the modern transportation needs and the aesthetical requirements of the society. These structures however are always lively with low stiffness, low mass, low damping and low natural frequencies. As a consequence, they are prone to vibration induced by human activities and can suffer severe vibration serviceability problems, particularly in the lateral direction. Pedestrian bridges are designed according to first and second limit states, these are the criteria involved in response to static design load. However, it is necessary to assess the dynamic response of bridge design load on pedestrians and assess it impact on the comfort of the user movement. Usually the load is considered a person or a small group which can be assumed in perfect motion synchronization. Already one person or small group can excite significant vibration of the deck. In order to calculate the dynamic response to the movement of people, designer needs available and suitable computational model and criteria. For the calculation program ANSYS based on finite element method was used.

Keywords: Footbridge, dynamic analysis, vibration serviceability of footbridges, lateral vibration, stiffness, dynamic force, walking force, slender suspension footbridges, natural frequencies and vibration modes, rhythm jumping, normal walking.

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512 Numerical Analysis of Wind Loads on a Hemicylindrical Roof Building

Authors: Marco Raciti Castelli, Sergio Toniato, Ernesto Benini

Abstract:

The flow field over a three dimensional pole barn characterized by a cylindrical roof has been numerically investigated. Wind pressure and viscous loads acting on the agricultural building have been analyzed for several incoming wind directions, so as to evaluate the most critical load condition on the structure. A constant wind velocity profile, based on the maximum reference wind speed in the building site (peak gust speed worked out for 50 years return period) and on the local roughness coefficient, has been simulated. In order to contemplate also the hazard due to potential air wedging between the stored hay and the lower part of the ceiling, the effect of a partial filling of the barn has been investigated. The distribution of wind-induced loads on the structure have been determined, allowing a numerical quantification of the effect of wind direction on the induced stresses acting on a hemicylindrical roof.

Keywords: CFD, wind, building, hemicylindrical roof.

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511 Hydrodynamic Analysis of Reservoir Due to Vertical Component of Earthquake Using an Analytical Solution

Authors: M. Pasbani Khiavi, M. A. Ghorbani

Abstract:

This paper presents an analytical solution to get a reliable estimation of the hydrodynamic pressure on gravity dams induced by vertical component earthquake when solving the fluid and dam interaction problem. Presented analytical technique is presented for calculation of earthquake-induced hydrodynamic pressure in the reservoir of gravity dams allowing for water compressibility and wave absorption at the reservoir bottom. This new analytical solution can take into account the effect of bottom material on seismic response of gravity dams. It is concluded that because the vertical component of ground motion causes significant hydrodynamic forces in the horizontal direction on a vertical upstream face, responses to the vertical component of ground motion are of special importance in analysis of concrete gravity dams subjected to earthquakes.

Keywords: Dam, Reservoir, Analytical solution, Vertical component, Earthquake

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510 Sensing Characteristics to Acid Vapors of a TPPS Coated Fiber Optic: A Preliminary Analysis

Authors: A. Bahrampour, A. Iadicicco, G. De Luca, M. Giordano, A. Cutolo, L. Monsù Scolaro, A. Cusano

Abstract:

In this work we report on preliminary analysis of a novel optoelectronic gas sensor based on an optical fiber integrated with a tetrakis(4-sulfonatophenyl)porphyrin (TPPS) thin film. The sensitive materials are selectively deposited on the core region of a fiber tip by UV light induced deposition technique. A simple and cheap process which can be easily extended to different porphyrin derivatives. When the TPPS film on the fiber tip is exposed to acid and/or base vapors, dramatic changes occur in the aggregation structure of the dye molecules in the film, from J- to H-type, resulting in a profound modification of their corresponding reflectance spectra. From the achieved experimental results it is evident that the presence of intense and narrow band peaks in the reflected spectra could be monitored to detect hazardous vapors.

Keywords: Optical fiber sensor, Porphyrins, Thin films UV induced deposition, TPPS.

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509 A Further Improvement on the Resurrected Core-Spreading Vortex Method

Authors: M-J. Huang, C-J. Huang, L-C. Chen

Abstract:

In a previously developed fast vortex method, the diffusion of the vortex sheet induced at the solid wall by the no-slip boundary conditions was modeled according to the approximation solution of Koumoutsakos and converted into discrete blobs in the vicinity of the wall. This scheme had been successfully applied to a simulation of the flow induced with an impulsively initiated circular cylinder. In this work, further modifications on this vortex method are attempted, including replacing the approximation solution by the boundary-element-method solution, incorporating a new algorithm for handling the over-weak vortex blobs, and diffusing the vortex sheet circulation in a new way suitable for high-curvature solid bodies. The accuracy is thus largely improved. The predictions of lift and drag coefficients for a uniform flow past a NASA airfoil agree well with the existing literature.

Keywords: Resurrected core-spreading vortex method, Boundaryelement method, Vortex sheet, Over-weak vortex blobs.

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508 Numerical Study on the Cavity-Induced Piping Failure of Embankment

Authors: H. J. Kim, G. C. Park, K. C. Kim, J. H. Shin

Abstract:

Cavities are frequently found beneath conduits on pile foundations in old embankments. Cavity reduces seepage length significantly and consequently causes piping failure of embankments. Case studies of embankment failures indicate that the relative settlement between ground and pile supported-concrete conduit was the main reason of the cavity. In this paper, an attempt to simulate the cavity-induced piping failure mechanism was made using finite element numerical method. Piping potential is examined by carrying out parametric study for influencing factors such as cavity length, water level, and flow conditions. The concentration of hydraulic gradient adjacent to cavity was found. It is found that the hydraulic gradient close to the cavity exceeds considerably the critical hydraulic gradient causing piping. Piping failure potential due to the existence of cavity is evaluated and contour map for the potential risk of an embankment for piping failure is proposed.

Keywords: Cavity, Embankment, Hydraulic gradient, Piping.

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507 Cr Induced Magnetization in Zinc-Blende ZnO Based Diluted Magnetic Semiconductors

Authors: Bakhtiar Ul Haq, R. Ahmed, A. Shaari, Mazmira Binti Mohamed, Nisar Ali

Abstract:

The capability of exploiting the electronic charge and spin properties simultaneously in a single material has made diluted magnetic semiconductors (DMS) remarkable in the field of spintronics. We report the designing of DMS based on zinc-blend ZnO doped with Cr impurity. The full potential linearized augmented plane wave plus local orbital FP-L(APW+lo) method in density functional theory (DFT) has been adapted to carry out these investigations. For treatment of exchange and correlation energy, generalized gradient approximations have been used. Introducing Cr atoms in the matrix of ZnO has induced strong magnetic moment with ferromagnetic ordering at stable ground state. Cr:ZnO was found to favor the short range magnetic interaction that reflect tendency of Cr clustering. The electronic structure of ZnO is strongly influenced in the presence of Cr impurity atoms where impurity bands appear in the band gap.

Keywords: ZnO, Density functional theory, Diluted magnetic semiconductors, Ferromagnetic materials, FP-L(APW+lo).

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506 A Wall Law for Two-Phase Turbulent Boundary Layers

Authors: Dhahri Maher, Aouinet Hana

Abstract:

The presence of bubbles in the boundary layer introduces corrections into the log law, which must be taken into account. In this work, a logarithmic wall law was presented for bubbly two phase flows. The wall law presented in this work was based on the postulation of additional turbulent viscosity associated with bubble wakes in the boundary layer. The presented wall law contained empirical constant accounting both for shear induced turbulence interaction and for non-linearity of bubble. This constant was deduced from experimental data. The wall friction prediction achieved with the wall law was compared to the experimental data, in the case of a turbulent boundary layer developing on a vertical flat plate in the presence of millimetric bubbles. A very good agreement between experimental and numerical wall friction prediction was verified. The agreement was especially noticeable for the low void fraction when bubble induced turbulence plays a significant role.

Keywords: Bubbly flows, log law, boundary layer.

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505 Characterization of the LMOS with Different Channel Structure

Authors: Hung-Pei Hsu, Jyi-Tsong Lin, Po-Hsieh Lin, Cheng-Hsien Chang, Ming-Tsung Shih, Chan-Hsiang Chang, Shih-Chuan Tseng, Min-Yan Lin, Shih-Wen Hsu

Abstract:

In this paper, we propose a novel metal oxide semiconductor field effect transistor with L-shaped channel structure (LMOS), and several type of L-shaped structures are also designed, studied and compared with the conventional MOSFET device for the same average gate length (Lavg). The proposed device electrical characteristics are analyzed and evaluated by three dimension (3-D) ISE-TCAD simulator. It can be confirmed that the LMOS devices have higher on-state drain current and both lower drain-induced barrier lowering (DIBL) and subthreshold swing (S.S.) than its conventional counterpart has. In addition, the transconductance and voltage gain properties of the LMOS are also improved.

Keywords: Average gate length (Lavg), drain-induced barrier lowering (DIBL), L-shaped channel MOSFET (LMOS), subthreshold swing (S.S.).

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504 Numerical Studies on Thrust Vectoring Using Shock-Induced Self Impinging Secondary Jets

Authors: S. Vignesh, N. Vishnu, S. Vigneshwaran, M. Vishnu Anand, Dinesh Kumar Babu, V. R. Sanal Kumar

Abstract:

Numerical studies have been carried out using a validated two-dimensional standard k-omega turbulence model for the design optimization of a thrust vector control system using shock induced self-impinging supersonic secondary double jet. Parametric analytical studies have been carried out at different secondary injection locations to identifying the highest unsymmetrical distribution of the main gas flow due to shock waves, which produces a desirable side force more lucratively for vectoring. The results from the parametric studies of the case on hand reveal that the shock induced self-impinging supersonic secondary double jet is more efficient in certain locations at the divergent region of a CD nozzle than a case with supersonic single jet with same mass flow rate. We observed that the best axial location of the self-impinging supersonic secondary double jet nozzle with a given jet interaction angle, built-in to a CD nozzle having area ratio 1.797, is 0.991 times the primary nozzle throat diameter from the throat location. We also observed that the flexible steering is possible after invoking ON/OFF facility to the secondary nozzles for meeting the onboard mission requirements. Through our case studies we concluded that the supersonic self-impinging secondary double jet at predesigned jet interaction angle and location can provide more flexible steering options facilitating with 8.81% higher thrust vectoring efficiency than the conventional supersonic single secondary jet without compromising the payload capability of any supersonic aerospace vehicle.

Keywords: Fluidic thrust vectoring, rocket steering, self-impinging secondary supersonic jet, TVC in aerospace vehicles.

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503 Thermal Cracking Respone of Reinforced Concrete Beam to Gradient Temperature

Authors: L. Dahmani, M.Kouane

Abstract:

In this paper are illustrated the principal aspects connected with the numerical evaluation of thermal stress induced by high gradient temperature in the concrete beam. The reinforced concrete beam has many advantages over steel beam, such as high resistance to high temperature, high resistance to thermal shock, Better resistance to fatigue and buckling, strong resistance against, fire, explosion, etc. The main drawback of the reinforced concrete beam is its poor resistance to tensile stresses. In order to investigate the thermal induced tensile stresses, a numerical model of a transient thermal analysis is presented for the evaluation of thermo-mechanical response of concrete beam to the high temperature, taking into account the temperature dependence of the thermo physical properties of the concrete like thermal conductivity and specific heat.

Keywords: Cracking, Gradient Temperature, Reinforced Concrete beam, Thermo-mechanical analysis.

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502 Phyllantus niruri Protects against Fe2+ and SNP Induced Oxidative Damage in Mitochondrial Enriched Fractions of Rats Brain

Authors: Olusola Olalekan Elekofehinti, Isaac Gbadura Adanlawo, Joao Batista Teixeira Rocha

Abstract:

The potential neuroprotective effect of Phyllantus nuriri against Fe2+ and sodium nitroprusside (SNP) induced oxidative stress in mitochondria of rats brain was evaluated. Cellular viability was assessed by MTT reduction, reactive oxygen species (ROS) generation was measured using the probe 2,7-dichlorofluoresce indiacetate (DCFH-DA). Glutathione content was measured using dithionitrobenzoic acid (DTNB). Fe2+ (10μM) and SNP (5μM) significantly decreased mitochondrial activity, assessed by MTT reduction assay, in a dose-dependent manner, this occurred in parallel with increased glutathione oxidation, ROS production and lipid peroxidation end-products (thiobarbituric acid reactive substances, TBARS). The co-incubation with methanolic extract of Phyllantus nuriri (10-200 μg/ml) reduced the disruption of mitochondrial activity, gluthathione oxidation, ROS production as well as the increase in TBARS levels caused by both Fe2+ and SNP in a dose dependent manner. HPLC analysis of the extract revealed the presence of gallic acid (20.540.01), caffeic acid (7.930.02), rutin (25.310.05), quercetin (31.280.03) and kaemferol (14.360.01). This result suggests that these phytochemicals account for the protective actions of P. niruri against Fe2+ and SNP -induced oxidative stress. Our results show that P. nuriri consist important bioactive molecules in the search for an improved therapy against the deleterious effects of Fe2+, an intrinsic producer of reactive oxygen species (ROS), that leads to neuronal oxidative stress and neurodegeneration.

Keywords: Phyllantus niruri, mitochondria, antioxidant, oxidative stress, synaptosome.

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501 Dynamic Response Analyses for Human-Induced Lateral Vibration on Congested Pedestrian Bridges

Authors: M. Yoneda

Abstract:

In this paper, a lateral walking design force per person is proposed and compared with Imperial College test results. Numerical simulations considering the proposed walking design force which is incorporated into the neural-oscillator model are carried out placing much emphasis on the synchronization (the lock-in phenomenon) for a pedestrian bridge model with the span length of 50 m. Numerical analyses are also conducted for an existing pedestrian suspension bridge. As compared with full scale measurements for this suspension bridge, it is confirmed that the analytical method based on the neural-oscillator model might be one of the useful ways to explain the synchronization (the lock-in phenomenon) of pedestrians being on the bridge.

Keywords: Pedestrian bridge, human-induced lateral vibration, neural-oscillator, full scale measurement, dynamic response analysis.

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500 Protective Effect of Saponin Extract from the Root of Garcinia kola (Bitter kola) against Paracetamol- Induced Hepatotoxicity in Albino Rats

Authors: Yemisi Rufina Alli Smith, Isaac Gbadura Adanlawo

Abstract:

Liver disorders are one of the major problems of the world. Despite its frequent occurrence, high morbidity and high mortality, its medical management is currently inadequate. This study was designed to evaluate the hepatoprotective effect of saponin extract of the root of Garcinia kola on the integrity of the liver of paracetamol induced wistar albino rats. Twenty five (25) male adult wistar albino rats were divided into five (5) groups. Group I was the Control group that received distilled water only, group II was the negative control that received 2 g/kg of paracetamol on the 13th day, and group III, IV and V were pre-treated with 100, 200 and 400mg/kg of the saponin extract before inducing the liver damage on the 13th day with 2 g/kg of paracetamol. Twenty four (24) h after administration, the rats were sacrificed and blood samples were collected. The serum Alanine Transaminase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) activities, Bilirubin and conjugated bilirubin, glucose and protein concentrations were evaluated. The liver was fixed immediately in Formalin and was processed and stained in Haematoxylin and Eosin (H&E). Administration of saponin extract from the root of Garcinia kola significantly decreased paracetamol induced elevated enzymes in the test group. Also histological observations showed that saponin extract of the root of Garcinia kola exhibited a significant liver protection against the toxicant as evident by the cells trying to return to normal. Saponin extract from the root of Garcinia kola indicated a protection of structural integrity of the hepatocytic cell membrane and regeneration of the damaged liver.

Keywords: Garcinia kola, Hepatoprotective, paracetamol, Saponin.

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499 Assessment of Landslide Volume for Alishan Highway Based On Database of Rainfall-Induced Slope Failure

Authors: Yun-Yao Chi, Ya-Fen Lee

Abstract:

In this paper, a study of slope failures along the Alishan Highway is carried out. An innovative empirical model is developed based on 15-year records of rainfall-induced slope failures. The statistical models are intended for assessing the volume of landslide for slope failure along the Alishan Highway in the future. The rainfall data considered in the proposed models include the effective cumulative rainfall and the critical rainfall intensity. The effective cumulative rainfall is defined at the point when the curve of cumulative rainfall goes from steep to flat. Then, the rainfall thresholds of landslide are established for assessing the volume of landslide and issuing warning and/or closure for the Alishan Highway during a future extreme rainfall. Slope failures during Typhoon Saola in 2012 demonstrate that the new empirical model is effective and applicable to other cases with similar rainfall conditions.

Keywords: Slope failure, landslide, volume, model, rainfall thresholds.

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498 Ficus deltoidea Extract Protects HaCaT Keratinocytes from UVB Irradiation-Induced Inflammation

Authors: Rosnani Hasham, Hyun Kyung Choi, Chang Seo Park

Abstract:

Ficus deltoidea from the Moraceae family is a popular medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory properties. In the present study, the anti-inflammatory effects of F. deltoidea extract on UVB-irradiated HaCaT Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated (12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of F. deltoidea extract. Cell viability following UVB irradiation was significantly higher in the groups treated with the F. deltoidea extract at doses of 0.05, 0.08 or 0.1% than in control group with UVB irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play primary roles in the inflammation process upon UV irradiation and are known to be stimulated by UVB irradiation. Treatment with the F. deltoidea extract dramatically inhibited the UV-induced TNF-α, IL-1α, IL-6, and COX-2 expression. These results suggest that the F. deltoidea extract inhibits the production of pro-inflammatory cytokines and may be an effective protective agent for the treatment of skin diseases.

Keywords: Ficus deltoidea, anti-inflammatory activity, cytokines, COX-2.

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497 PIIN Suppression Using Random Diagonal Code for Spectral Amplitude Coding Optical CDMA System

Authors: Hilal Adnan Fadhil, Syed Alwei, R. Badlishah Ahmad

Abstract:

A new code for spectral-amplitude coding optical code-division multiple-access system is proposed called Random diagonal (RD) code. This code is constructed using code segment and data segment. One of the important properties of this code is that the cross correlation at data segment is always zero, which means that Phase Intensity Induced Noise (PIIN) is reduced. For the performance analysis, the effects of phase-induced intensity noise, shot noise, and thermal noise are considered simultaneously. Bit-error rate (BER) performance is compared with Hadamard and Modified Frequency Hopping (MFH) codes. It is shown that the system using this new code matrices not only suppress PIIN, but also allows larger number of active users compare with other codes. Simulation results shown that using point to point transmission with three encoded channels, RD code has better BER performance than other codes, also its found that at 0 dbm PIIN noise are 10-10 and 10-11 for RD and MFH respectively.

Keywords: OCDMA, MFH, PIIN, and BER.

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496 Possible Protective Effect of Kombucha Tea Ferment on Cadmium Chloride Induced Liver and Kidney Damage in Irradiated Rats

Authors: Nashwa Kamel Ibrahim

Abstract:

Kombucha Tea Ferment (KT), was given to male albino rats, (1ml/Kg of body weight), via gavages, during 2 weeks before intraperitoneal administration of 3.5 mg/Kg body weight CdCl2 and/or whole body γ-irradiation with 4Gy, and during 4 weeks after each treatment. Hepatic and nephritic pathological changes included significant increases of serum alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) activities, and creatinine and urea contents with significant decrease in serum total antioxidant capacity (TAC). Increase in oxidative stress markers in liver and kidney tissues expressed by significant increase in malondialdehyde (MDA) and nitric oxide (NO) contents associated to significant depletion in superoxide dismutase (SOD) and catalase (CAT) activities, and reduced glutathione (GSH) content were recorded. KT administration results in recovery of all the pathological changes. It could be concluded that KT might protect liver and kidney from oxidative damage induced by exposure to cadmium and/ or γ-irradiation.

Keywords: Cadmium, Kombucha, radiation, rats

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495 In vitro Propagation of Purple Nutsedge (Cyperus rotundus L.) for Useful Chemical Extraction

Authors: Chockpisit Thepsithar, Nongnuch Euawong, Nukul Jonghomkajorn

Abstract:

The in vitro culture procedure of purple nutsedge (Cyperus rotundus L.) for multiple shoot induction and tuber formation was established. Multiple shoots were significantly induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige and Skoog (MS) medium supplemented with 4.44 μM 6- benzyladinine (BA) alone or in combination with 2.85 μM 1- indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of culturing. Moreover, MS medium supplemented with 4.44 μM BA and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9 tubers with 3.4 rhizomes per explant. In combination with ancymidol and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5 tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively. However, MS medium containing 3.9 or 7.8 μM ancymidol in combination with either 60 or 80 g/L sucrose enchanced significant root formation at 20.9 – 23.6 roots per explant.

Keywords: Purple nutsedge, Cyperus rotundus, multiple shoot induction, tuber formation

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494 Olive Leaves Extract Restored the antioxidant Perturbations in Red Blood Cells Hemolysate in Streptozotocin Induced Diabetic Rats

Authors: Ismail I. Abo Ghanema, Kadry M. Sadek

Abstract:

Oxidative stress and overwhelming free radicals associated with diabetes mellitus are likely to be linked with development of certain complication such as retinopathy, nephropathy and neuropathy. Treatment of diabetic subjects with antioxidant may be of advantage in attenuating these complications. Olive leaf (Oleaeuropaea), has been endowed with many beneficial and health promoting properties mostly linked to its antioxidant activity. This study aimed to evaluate the significance of supplementation of Olive leaves extract (OLE) in reducing oxidative stress, hyperglycemia and hyperlipidemia in Sterptozotocin (STZ)- induced diabetic rats. After induction of diabetes, a significant rise in plasma glucose, lipid profiles except High density lipoproteincholestrol (HDLc), malondialdehyde (MDA) and significant decrease of plasma insulin, HDLc and Plasma reduced glutathione GSH as well as alteration in enzymatic antioxidants was observed in all diabetic animals. During treatment of diabetic rats with 0.5g/kg body weight of Olive leaves extract (OLE) the levels of plasma (MDA) ,(GSH), insulin, lipid profiles along with blood glucose and erythrocyte enzymatic antioxidant enzymes were significantly restored to establish values that were not different from normal control rats. Untreated diabetic rats on the other hand demonstrated persistent alterations in the oxidative stress marker (MDA), blood glucose, insulin, lipid profiles and the antioxidant parameters. These results demonstrate that OLE may be of advantage in inhibiting hyperglycemia, hyperlipidemia and oxidative stress induced by diabetes and suggest that administration of OLE may be helpful in the prevention or at least reduced of diabetic complications associated with oxidative stress.

Keywords: Diabetes mellitus, olive leaves, oxidative stress, red blood cells

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493 A Study on the Effects of Prolactin and Its Abnormalities on Semen Parameters of Male White Rats

Authors: Rizvi Hasan

Abstract:

Male factor infertility due to endocrine disturbances such as abnormalities in prolactin levels are encountered in a significant proportion. This case control study was carried out to determine the effects of prolactin on the male reproductive tract, using 200 male white rats. The rats were maintained as the control group (G1), hypoprolactinaemic group (G2), 3 hyperprolactinaemic groups induced using oral largactil (G3), low dose fluphenazine (G4) and high dose fluphenazine (G5). After 100 days, rats were subjected to serum prolactin (PRL) level measurements and for basic seminal fluid analysis (BSA). The difference between serum PRL concentrations of rats in G2, G3, G4 and G5 as compared to the control group were highly significant by Student’s t-test (p<0.001). There were statistically significant differences in seminal fluid characteristics of rats with induced prolactin abnormalities when compared with those of control group (p value <0.05), effects were more marked as the PRL levels rise.

Keywords: Male factor infertility, Prolactin, Seminal fluid analysis, animal studies.

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492 Spectral Analysis of Radiation-Induced Natural Convection in Littoral Waters

Authors: Yadan Mao, Chengwang Lei, John C. Patterson

Abstract:

The mixing of pollutions and sediments in near shore regions of natural water bodies depends heavily on the characteristics such as the strength and frequency of flow instability. In the present paper, the instability of natural convection induced by absorption of solar radiation in littoral regions is considered. Spectral analysis is conducted on the quasi-steady state flow to reveal the power and frequency modes of the instability at various positions. Results indicate that the power of instability, the number of frequency modes, the prominence of higher frequency modes, and the highest frequency mode increase with the offshore distance and/or Rayleigh number. Harmonic modes are present at relatively low Rayleigh numbers. For a given offshore distance, the position with the strongest power of instability is located adjacent to the sloping bottom while the frequency modes are the same over the local depth. As the Rayleigh number increases, the unstable region extends toward the shore.

Keywords: Instability, Littoral waters, natural convection, Spectral analysis

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491 Development of All-male Fingerlings by Heat Treatment and the Genetic Mechanism of Heat Induced Sex Determination in Nile Tilapia(Oreochromis niloticus L.)

Authors: P. O. Angienda, B. O. Aketch, E. N. Waindi

Abstract:

Juvenile Nile tilapia subjected to heat treatment at temperatures ranging from 260C to 370C showed positive correlation (P<0.01) between treatment temperatures and resultant sex ratios, while, survival rate of the fry showed a negative correlation against temperature (P<0.01). The optimal temperature for both sex shift towards males and survival rates was 36±0.5°C, producing male percentage of 86.31 and a fry survival of 65.25. To determine the genetic basis of temperature sex-determination in Nile tilapia, we employed three microsatellite markers (Abur36, Abur100 and UNH846). Abur36 predicted the sex of 95% of the heat induced individuals, suggesting that the locus influence sex ratio and its interaction with temperature result in male biased sex ratio. This locus could turn out to be the major sex determining gene operating in Nile tilapia. These markers could be used in marker-assisted selection to select genotypes that give a higher percentage of males for commercial production.

Keywords: Heat treatment, Microsatellite, Nile tilapia, sex-determination.

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490 Comparative Micro-Morphology, Anatomy and Architecture of Leaf of Physalis

Authors: Chockpisit Thepsithar, Aree Thongpukdee

Abstract:

Two species of Physalis, P.angulataL. and P. peruviana L. were used as models for comparative study to understand the values of micro-morphological, -anatomical and architectural characteristics of leaf for taxonomic purposes and possibly breeding and commercial applications. Both speciespossess amphistomaticleaves with 1-layer epidermis, 3-4-layer spongy mesophyll andbicollateral bundle midrib. Palisade parenchyma cells of P. angulatawere almost twice longer (65-75 μm) than the other one. Type of stomata was similar as anomocyticbut stomatal index(SI) at adaxial surface and abaxial surface of P. angulata were less than of P. peruvianaas 3.57, 4.00 and6.25, 6.66 respectively. Some leaf architectural characteristics such as leaf shape, order of venationalsoprovided information of taxonomic significance

Keywords: Physalis, Solanaceae, micromorphology, anatomy, leaf architecture.

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489 Aqueous Extract of Flacourtia indica Prevents Carbon Tetrachloride Induced Hepatotoxicity in Rat

Authors: Gnanaprakash K, Madhusudhana Chetty C, Ramkanth S, Alagusundaram M, Tiruvengadarajan VS, Angala Parameswari S, Mohamed Saleem TS

Abstract:

Carbon tetrachloride (CCl4) is a well-known hepatotoxin and exposure to this chemical is known to induce oxidative stress and causes liver injury by the formation of free radicals. Flacourtia indica commonly known as 'Baichi' has been reported as an effective remedy for the treatment of a variety of diseases. The objective of this study was to investigate the hepatoprotective activity of aqueous extract of leaves of Flacourtia indica against CCl4 induced hepatotoxicity. Animals were pretreated with the aqueous extract of Flacourtia indica (250 & 500 mg/kg body weight) for one week and then challenged with CCl4 (1.5 ml/kg bw) in olive oil (1:1, v/v) on 7th day. Serum marker enzymes (ALP, AST, ALT, Total Protein & Total Bilirubin) and TBARS level (Marker for oxidative stress) were estimated in all the study groups. Alteration in the levels of biochemical markers of hepatic damage like AST, ALT, ALP, Total Protein, Total Bilirubin and lipid peroxides (TBARS) were tested in both CCl4 treated and extract treated groups. CCl4 has enhanced the AST, ALT, ALP and the Lipid peroxides (TBARS) in liver. Treatment of aqueous extract of Flacourtia indica leaves (250 & 500 mg/kg) exhibited a significant protective effect by altering the serum levels of AST, ALT, ALP, Total Protein, Total Bilirubin and liver TBARS. These biochemical observations were supported by histopathological study of liver sections. From this preliminary study it has been concluded that the aqueous extract of the leaves of Flacourtia indica protects liver against oxidative damages and could be used as an effective protector against CCl4 induced hepatic damage. Our findings suggested that Flacourtia indica possessed good hepatoprotective activity

Keywords: Carbon Tetrachloride, Flacourtia indica, Hepatoprotective activity, Oxidative stress

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488 Performance of Subcarrier- OCDMA System with Complementary Subtraction Detection Technique

Authors: R. K. Z. Sahbudin, M. K. Abdullah, M. Mokhtar, S. B. A. Anas, S. Hitam

Abstract:

A subcarrier - spectral amplitude coding optical code division multiple access system using the Khazani-Syed code with Complementary subtraction detection technique is proposed. The proposed system has been analyzed by taking into account the effects of phase-induced intensity noise, shot noise, thermal noise and intermodulation distortion noise. The performance of the system has been compared with the spectral amplitude coding optical code division multiple access system using the Hadamard code and the Modified Quadratic Congruence code. The analysis shows that the proposed system can eliminate the multiple access interference using the Complementary subtraction detection technique, and hence improve the overall system performance.

Keywords: Complementary subtraction, Khazani-Syed code, multiple access interference, phase-induced intensity noise

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