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Ficus deltoidea Extract Protects HaCaT Keratinocytes from UVB Irradiation-Induced Inflammation

Authors: Rosnani Hasham, Hyun Kyung Choi, Chang Seo Park


Ficus deltoidea from the Moraceae family is a popular medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory properties. In the present study, the anti-inflammatory effects of F. deltoidea extract on UVB-irradiated HaCaT Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated (12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of F. deltoidea extract. Cell viability following UVB irradiation was significantly higher in the groups treated with the F. deltoidea extract at doses of 0.05, 0.08 or 0.1% than in control group with UVB irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play primary roles in the inflammation process upon UV irradiation and are known to be stimulated by UVB irradiation. Treatment with the F. deltoidea extract dramatically inhibited the UV-induced TNF-α, IL-1α, IL-6, and COX-2 expression. These results suggest that the F. deltoidea extract inhibits the production of pro-inflammatory cytokines and may be an effective protective agent for the treatment of skin diseases.

Keywords: Cytokines, anti-inflammatory activity, COX-2, Ficus deltoidea

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[1] K. B. Beckman, and B. N. Ames, “The free radical theory of aging matures,”. Physiol. Rev., vol. 78, pp. 547–581, 1998.
[2] S. Pillai, C. Oresajo, and J. Hayward, “Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation — a review,”. Int. J. Cosmet. Sci., vol. 27, pp. 17–34, 2005.
[3] A. Pupe, R. Moison, P. De Haes, G. B. Van Henegouwen, L. Rhodes, H. Degreef, and M. Garmyn, “Eicosapentaenoic acid, a n-3 polyunsaturated fatty acid differentially modulates TNF-alpha, IL-1alpha, IL-6 and PGE2 expression in UVB irradiated human keratinocytes,”. J. Invest. Dermatol., vol. 118, pp. 692–698, 2002.
[4] C. E. M. Griffiths, G. Russman, G. Majmudar, R. S. Singer, T. A. Hamilton, and J. J. Voorhees, “Restoration of collagen formation in photodamaged human skin by tretinoin (retinoic acid),”. N. Engl. J. Med., vol. 329, pp. 530-534, 1993.
[5] L. J. McCawley, and L. M. Matrisian, “Matrix metalloproteinases: they're not just for matrix anymore,”. Curr. Opin. Cell. Biol., vol. 13, pp. 534–540, 2001.
[6] E. J. Jung, J. Lee, J. Baek, K. Jung, S. Huh, S. Kim, J. Koh, and D. Park, “Effect of Camellia japonica oil on human type I procollagen production and skin barrier function,”. J. Ethnopharmacol., vol. 112, pp. 127–131, 2007.
[7] S. R. Pinnell, “Cutaneous photodamage, oxidative stress, and topical antioxidant protection”. J. Am. Acad. Dermatol., vol. 48, pp. 1–19, 2003.
[8] I. A. Jahan, N. Nahar, M. Mosihuzzaman, B. Rokeya, L. Ali, A. K. Azad Khan, T. Makhmur, and M. Iqbal Choudhary, “Hypoglycaemic and antioxidant activities of Ficus racemosa Linn. Fruits,”. Nat. Prod. Res., vol. 23, pp. 399-408, 2009.
[9] R. K. Singh, S. Mehta, D. Jaiswal, P. K. Rai, and G. Watal, “Antidiabetic effect of Ficus bengalensis aerial roots in experimental animals,”. J. Ethnopharmacol., vol. 123, pp. 110-114, 2009.
[10] A. Grone, “Keratinocytes and cytokines,”. Vet. Immunol. Immunopathol., vol. 88, pp. 1–12, 2002.
[11] L. Rittie, and G. J. Fisher, “UV-light-induced signal cascades and skin aging,”. Ageing Res. Rev., vol. 1, pp. 705–720, 2002.
[12] Z. Adam, M. Hamid, A. Ismail, and S. Khamis, “Effect of Ficus deltoidea extract on hepatic basal and insulin-stimulated glucose uptake,”. J. Biol. Sci., vol. 9, pp. 796-803, 2009.
[13] M. R. Sulaiman, M. K. Hussain, Z. A. Zakaria, M. N. Somchit, S. Moin, A. S. Mohamad, and D. A. Israf, “Evaluation of the antinociceptive activity of Ficus deltoidea aqueous extract,”. Fitoterapia, vol. 79, pp. 557-561, 2008.
[14] M. J. Oh, M. Abdul Hamid, S. Ngadiran, Y. K. Seo, M. R. Sarmidi, and C. S. Park, “Ficus deltoidea (Mas cotek) extract exerted antimelanogenic activity by preventing tyrosinase activity in vitro and by suppressing tyrosinase gene expression in B16F1 melanoma cells,”. Arch. Dermatol. Res., vol. 303, pp. 161-170, 2010.
[15] Z. Abdullah, H. Khalid, I. Zhari, and M. A. Rasadah, “Antiinflammatory activity of standardized extracts of leaves of three varieties of Ficus deltoidea,”. Int. J. Pharm. Clin. Res., vol. 1, pp. 100-105, 2009.