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Possible Protective Effect of Kombucha Tea Ferment on Cadmium Chloride Induced Liver and Kidney Damage in Irradiated Rats

Authors: Nashwa Kamel Ibrahim


Kombucha Tea Ferment (KT), was given to male albino rats, (1ml/Kg of body weight), via gavages, during 2 weeks before intraperitoneal administration of 3.5 mg/Kg body weight CdCl2 and/or whole body γ-irradiation with 4Gy, and during 4 weeks after each treatment. Hepatic and nephritic pathological changes included significant increases of serum alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) activities, and creatinine and urea contents with significant decrease in serum total antioxidant capacity (TAC). Increase in oxidative stress markers in liver and kidney tissues expressed by significant increase in malondialdehyde (MDA) and nitric oxide (NO) contents associated to significant depletion in superoxide dismutase (SOD) and catalase (CAT) activities, and reduced glutathione (GSH) content were recorded. KT administration results in recovery of all the pathological changes. It could be concluded that KT might protect liver and kidney from oxidative damage induced by exposure to cadmium and/ or γ-irradiation.

Keywords: Cadmium, Kombucha, radiation, rats

Digital Object Identifier (DOI):

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[1] D.R. Spitz, E.I.Azzam, J.J. Li, and D. Gius, "Metabolic oxidation/reduction reactions and cellular responses to ionizing radiation: A unifying concept in stress response biology". Cancer Metast Rev vol. 23, pp. 311-322, 2004.
[2] J.Sun, Y. Chen, M. Li, and Z. Ge, "Role of antioxidant enzymes on ionizing radiation resistance". Free Radic Biol Med vol.24, pp. 586-93, 1998.
[3] A.Klos, "Lead, cadmium and mercury content in metals planned for consumption in selected kindergartens in Warsaw". Proceeding of the 4th International Scientific-Technical Conference, (STC-01), Warsaw, 2001, pp. 4-5.
[4] E.Kowalczyk, K. Anna, F. Pawel, K. Maria, N. Jan, B. Jan, K. Jozef, and T. Piotr, "Effect of anthocyanins on selected biochemical parameters in rats exposed to cadmium". Acta Biochem. Polonica vol.50, pp. 543-548, 2003.
[5] A.B.Ericson , Gallen, P.Weterholm , " Cigarette smoking as an etiological factor in cleft lip and palate". Am J Obstet Gynecol vol.135, pp. 348-351, 1979.
[6] M.Waisberg, P. Joseph, B. Hale, and D. Beyersmann, "Molecular and cellular mechanisms of cadmium carcinogenesis: a review". Toxicology vol.192, pp. 95-117, 2003.
[7] Z.A.Shaikh, T.A. Vu, and K. Zaman, "Oxidative stress as a mechanism of chronic cadmium-induced hepatotoxicity and renal toxicity and protection by antioxidants". Toxicol Appl Pharmacol vol.154, pp. 256- 263, 1999.
[8] E.Casalino, G. Calzaretti, C. Sblano, and C. Landriscina, "Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium". Toxicology vol.179, pp. 37-50, 2002.
[9] G. B.Morales, H.H. and Sanchez, "Manufacture of a beverage from cheese whey using "tea fungus" fermentation". Rev Latinoam Microbiol vol. 45, pp. 5, 2003.
[10] C.Dufresne , and E. Farnworth , "Tea, kombucha health: a review". Food Res Int vol. 336, pp. 409-421, 2000.
[11] T. Pauline , P. Dipti , B. Anju , S. Kavimani , S.K. Sharma , A.K. Kian , S.K. Sarada , M. Sairam , G. Ilavazhagan , K. Devendra , and W. Selvamurthy , "Studies on toxicity, anti-stress and hepato-protective properties of Kombucha tea". Biomed Environ Sci vol.14, no.3, pp. 207, 2001.
[12] M. Sai Ram, B. Anju , T. Pauline , P. Dipti , A. K Kian , S.S. Mongia , S.K. Sharma ,B. Singh , R. Singh , G. Ilavazhagan ,D. Kumar ,and W. Selvamurthy , " Effect of kombucha tea on chromate (VI)-induced oxidative stress in albino rats". J Ethnopharmacol vol.71no.1-2, pp.235, 2000.
[13] O. A. Gharib, "Does kombucha tea reduce the damage induced by radiation exposure?" Egypt J Rad Sci Applic vol.20no.1, pp. 141, 2007.
[14] K .N. Tzirogiannis, G. I. Panoutsopoulos, M. D. Demonakou, R. I .Hereti, K. N .Alexandropoulou, A. C. Basayannis, and M. G. Mykoniatis , "Time-course of cadmium-induced acute hepatotoxicity in the rat liver: the role of apoptosis". Arch Toxicol vol.77, pp. 694-701, 2003.
[15] S. Reitman, S. Frankel, "A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases". Am J Clin Pathol vol.28no.1, pp. 56, 1957.
[16] D. P. Kind, and E. G. King, 1954. Practical Clinical Biochemistry 1: H. Varley, AH Gownlock and M Bell, eds, 5th edition, William Heinmann Medical Books Ltd, London 1954, P. 892.
[17] D. Koracevic, G. Koracevic, V. Djordjevic, S. Andrejevic, and V. Cosic, "Method for the measurement of antioxidant activity in human fluid". J Clinc Pathol vol.54, no.5, pp.356, 2001.
[18] C. J. Halled, and J. G. Cook, "Reduced nicotinamide adenine dinucleotide-coupled reaction for emergency blood urea estimation". Clin Chim Acta vol. 35, pp.33-40, 1971.
[19] R. J. Henery, From Principle and Techniques: Clinical chemistry 2nd edition. New York: Harper & Row, 1974, pp.525.
[20] T. Yoshioka, K. Kawada, T. Shimada, and M. Mori, "Lipid peroxidation in maternal and cord blood and protective mechanism against activatedoxygen toxicity in the blood". Am J Obstet Gynecol vol.135,pp. 372, 1979.
[21] M. Minami, H. Yoshikawa, "A simplified assay method of superoxide dismutase". Clinica Acta vol.92, pp. 337, 1979.
[22] H. Aebi, "Catalase in vitro". Methods Enzymol vol.105, pp.121-6, 1984.
[23] E. Beutler, O. Duron, and B.M. Kelly, "Improved method for determination of blood glutathione". Lab Clin Med vol.61,no.5,pp.882, 1963.
[24] Y. Geng , M. Almqvist , and G.K. Hansson , "DNA cloning and expression of inducible nitric oxide synthase from rat vascular smooth muscle cells". Biochim Biophys Acta vol.1218, pp.421, 1994.
[25] T. Finkel, and N.J. Holbrook, "Oxidants, oxidative stress and biology of aging". Nature vol. 408, pp.239- 47, 2000.
[26] M.M. Kaplan. "Serum alkaline phosphatase - another piece is added to the puzzle". Hepato vol. 6, no.3, pp.526, 1986.
[27] K.C. Kregel, and H.J. Zhang, "An integrated view of oxidative stress in aging: basic mechanisms, functional effects, and pathological considerations". Am. J Physiol Regul Integr Comp Physiol vol. 292,pp. 18-36, 2007.
[28] D. Roy, K. Maya, M. Shawn, L. Larry, D. Christine, and J. Basil, "Dynamic responses of the glutathione system to acute oxidative stress in dystrophic mouse (mdx) muscles". Am J Physiol Regul Integr Comp Physiol vol.291, pp. 704-710, 2006.
[29] S. Yalin , U. Comclekoglu , S. Bagis , N.O. Sahin , O.O. Ogenler ,and R. Hatungil , " Acute effect of single- dose cadmium treatment on lipid peroxidation and antioxidant enzymes in ovriectomized rats". Ecotoxicol Environ Saf vol.65, pp.140-144, 2006.
[30] S. Sarkar, P.Yadav, R. Trivedi, A.K. Bansal, and D. Bhatnager , "Cadmium-induced lipid peroxidation and the status antioxidant system in rat tissues". J Trace Elem Med Biol vol.9, pp. 144-149, 1995.
[31] K. Mitsumori , M. Shibutani , S. Sato , H. Onodera , J. Nakagawa , Y. Hayashi ,and M. Ando, " Relationship between the development of hepato-renal toxicity and cadmium accumulation in rats given minimum to large amounts of cadmium chloride in the long-term: preliminary study". Arch Toxicol vol.72, pp.545-552, 1998.
[32] J.P. Groten, E.J. Sinkeldam, J.B. Luten, P.J. Bladeren, "Comparison of the toxicity of inorganic and liver-incorporated cadmium: A four week feeding study in rats". Food Chem. Toxicol vol.28,pp. 435-441,1990.
[33] M. Tanaka, M. Yanagi, K. Shirota, Y. Une , Y. Nomura , T. Masaoka ,and F. Akahori , " Effect of cadmium in the zinc deficient rat". Vet Hum Toxicol vol.37, pp. 203-208, 1995.
[34] S.J. Stohs, and D. Bagchi , " Oxidative mechanisms in the toxicity of metal ions". Free Rad Biol Med vol.18,pp. 321-336, 1995.
[35] H. Dally, and A. Hartwig, "Induction and repair inhibition of oxidative DNA damage by nickel (II) and cadmium (II) in mammalian cells". Carcinogenesis vol.18, pp. 1021-1026, 1997.
[36] P. O-Brien, and H.J. Salacinski, "Evidence that the reactions of cadmium in the presence of metallothionein can produce hydroxyl radicals". Arch Toxicol vol.72, pp. 690-700, 1998.
[37] J. Navarro, E. Obrador, J. Carretero, I. Petchen, J. Avino, P. Perez, and J.M. Estrela, "Changes in glutathione status and the antioxidant system in blood and in cancer cells associate with tumor growth in vivo". Free Radic Biol Med vol.26, no.3-4, pp.410, 1999.
[38] B.I. Ognjanović, S.Z. Pavlović, R.V.A. Ikić Tajn, S.D. Maletić, Z.S. Sai─ìić, and V.M. Petrović, "Protective influence of vitamin E on antioxidant defense system in the blood of rats treated with cadmium". Physiol Res vol.52, pp. 563-570, 2003.
[39] M. Nagoraj, S. Sunitha, and P. Varalaskhmi, "Effect of lupeol, a pentacyclic triterpene, on lipid peroxidation and antioxidant status in rat kidney after chronic cadmium exposure". J Applied Toxicol vol.20, pp.413-417, 2000.
[40] M.W. Fariss, "Cadmium toxicity: Unique cytoprotective properties of alpha tocopheryl succinate in hepatocytes". Toxicology vol.69, pp. 63- 77, 1991.
[41] S.M. Zaidi, T.M. Al-Qirim, and N. Banu, "Effects of antioxidants vitamins and glutathione depletion and lipid peroxidation induced by restraint stress in the rat liver". Drugs RD vol.6, no.3, pp.157, 2005.
[42] P.J. Blanc, "Characterization of the tea fungus metabolites", Biotechnol Lett vol.18, no.2, pp.139-143, 1996.