Influence of Calcium Intake Level to Osteoporptic Vertebral bone and Degenerated Disc in Biomechanical Study
The aim of the present study is to analyze the generation of osteoporotic vertebral bone induced by lack of calcium during growth period and analyze its effects for disc degeneration, based on biomechanical and histomorphometrical study. Mechanical and histomorphological characteristics of lumbar vertebral bones and discs of rats with calcium free diet (CFD) were detected and tracked by using high resolution in-vivo micro-computed tomography (in-vivo micro-CT), finite element (FE) and histological analysis. Twenty female Sprague-Dawley rats (6 weeks old, approximate weight 170g) were randomly divided into two groups (CFD group: 10, NOR group: 10). The CFD group was maintained on a refmed calcium-controlled semisynthetic diet without added calcium, to induce osteoporosis. All lumbar (L 1-L6) were scanned by using in vivo micro-CT with 35i.un resolution at 0, 4, 8 weeks to track the effects of CFD on the generation of osteoporosis. The fmdings of the present study indicated that calcium insufficiency was the main factor in the generation of osteoporosis and it induced lumbar vertebral disc degeneration. This study is a valuable experiment to firstly evaluate osteoporotic vertebral bone and disc degeneration induced by lack of calcium during growth period from a biomechanical and histomorphometrical point of view.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1061551Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1727
 F.R. Greer and N.F. Krebs, "Optimizing bone health and calcium intakes of infants, children, and adolescents" Pediatrics, vol. 117, no. 2, pp. 578-585, 2006.
 B.L. Langdahl, A.G. Loft, E.F. Eriksen, L. Mosekilde, P. Charles, "Bone mass, bone turnover, body composition, and calcium homeostasis in former hyperthyroid patients treated by combined medical therapy" Thyroid. vol. 6, no. 3, pp. 161-168, 1996.
 C. Gennari, "Calcium and vitamin D nutrition and bone disease of the elderly" Public health nutr.vol. 4, no. 2B, pp. 547-559, 2001.
 M. van der Klift and H. A. P. Pols, "Assessment of fracture risk: Who should be treated for osteoporosis?" Best Practice & Research Clinical Rheumatology, vol. 19, no. 6, pp. 937-950, 2005.
 C. Cooper, "Osteoporosis: An Increasing Public Health Problem" Medicographia, vol. 24, no. 4, pp. 277-284, 2002.
 P. D. Delmas, "Treatment of Postmenopausal Osteoporosis" The Lancet, vol. 359, no. 9322, pp.2018-2026, 2002.
 B. Kaplan, M. Hirsch, "Current approach to fracture prevention in postmenopausal osteoporosis" Clin Exp Obstet Gynecol, Vol. 31, no. 4, pp. 251-255, 2004.
 T. Hara, T. Sato, M. Oka, S. Mori and H. Shirai, "Effects of ovariectomy and/or dietary calcium deficiency on bone dynamics in the rat hard palate, mandible and proximal tibia" Arch. Oral. Biol, Vol. 46, no. 5, pp. 443-451, 2001.
 D. G. Woo, Y. Y. Won, H. S. Kim and D. Lim, "A Biomechanical Study of Osteoporotic Vertebral Trabecular Bone: The Use of Micro-CT and High-Resolution Finite Element Analysis" Journal of Mechanical Science and Technology, vol. 21, no. 4, pp. 593-601, 2007.
 L. Bresnahan, A.T. Ogden, R.N. Natarajan, R.G. Fessler, "A Biomechanical Evaluation of Graded Posterior Element Removal for Treatment of Lumbar Stenosis" Spine, Vol. 34, no. 1, pp. 17-23, 2008.