Search results for: Robust stability

Authors: Nikhil, Bestamin Özkaya, Ari Visa, Chiu-Yue Lin, Jaakko A. Puhakka, Olli Yli-Harja

Abstract:

The performance of a sucrose-based H2 production in a completely stirred tank reactor (CSTR) was modeled by neural network back-propagation (BP) algorithm. The H2 production was monitored over a period of 450 days at 35±1 ºC. The proposed model predicts H2 production rates based on hydraulic retention time (HRT), recycle ratio, sucrose concentration and degradation, biomass concentrations, pH, alkalinity, oxidation-reduction potential (ORP), acids and alcohols concentrations. Artificial neural networks (ANNs) have an ability to capture non-linear information very efficiently. In this study, a predictive controller was proposed for management and operation of large scale H2-fermenting systems. The relevant control strategies can be activated by this method. BP based ANNs modeling results was very successful and an excellent match was obtained between the measured and the predicted rates. The efficient H2 production and system control can be provided by predictive control method combined with the robust BP based ANN modeling tool.

Keywords: Back-propagation, biohydrogen, bioprocessmodeling, neural networks.

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Authors: S. Ritddech, P. Aroonjarattham, K. Aroonjarattham

Abstract:

The pullout strength had an effect on the stability of plate screw fixation when inserted in the cervical spine. Nine different titanium alloy bone screws were used to test the pullout strength through finite element analysis. The result showed that the Moss Miami I can bear the highest pullout force at 1,075 N, which causes the maximum von Mises stress at 858.87 MPa, a value over the yield strength of titanium. The bone screw should have large outer diameter, core diameter and proximal root radius to increase the pullout strength.

Keywords: Pullout strength, Screw parameter, Cervical spine, Finite element analysis.

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Authors: Jonghyun Park, Toan Nguyen Dinh, Gueesang Lee

Abstract:

In this paper, we present a novel approach to accurately detect text regions including shop name in signboard images with complex background for mobile system applications. The proposed method is based on the combination of text detection using edge profile and region segmentation using fuzzy c-means method. In the first step, we perform an elaborate canny edge operator to extract all possible object edges. Then, edge profile analysis with vertical and horizontal direction is performed on these edge pixels to detect potential text region existing shop name in a signboard. The edge profile and geometrical characteristics of each object contour are carefully examined to construct candidate text regions and classify the main text region from background. Finally, the fuzzy c-means algorithm is performed to segment and detected binarize text region. Experimental results show that our proposed method is robust in text detection with respect to different character size and color and can provide reliable text binarization result.

Keywords: Text detection, edge profile, signboard image, fuzzy clustering.

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Authors: Ahmad Sharieh, R Bremananth

Abstract:

Artificial Intelligence based gaming is an interesting topic in the state-of-art technology. This paper presents an automation of a tradition Omani game, called Al-Hawalees. Its related issues are resolved and implemented using artificial intelligence approach. An AI approach called mini-max procedure is incorporated to make a diverse budges of the on-line gaming. If number of moves increase, time complexity will be increased in terms of propositionally. In order to tackle the time and space complexities, we have employed a back propagation neural network (BPNN) to train in off-line to make a decision for resources required to fulfill the automation of the game. We have utilized Leverberg- Marquardt training in order to get the rapid response during the gaming. A set of optimal moves is determined by the on-line back propagation training fashioned with alpha-beta pruning. The results and analyses reveal that the proposed scheme will be easily incorporated in the on-line scenario with one player against the system.

Keywords: Artificial neural network, back propagation gaming, Leverberg-Marquardt, minimax procedure.

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Authors: Latha Parameswaran, K. Anbumani

Abstract:

Multimedia security is an incredibly significant area of concern. The paper aims to discuss a robust image watermarking scheme, which can withstand geometric attacks. The source image is initially moment normalized in order to make it withstand geometric attacks. The moment normalized image is wavelet transformed. The first level wavelet transformed image is segmented into blocks if size 8x8. The product of mean and standard and standard deviation of each block is computed. The second level wavelet transformed image is divided into 8x8 blocks. The product of block mean and the standard deviation are computed. The difference between products in the two levels forms the watermark. The watermark is inserted by modulating the coefficients of the mid frequencies. The modulated image is inverse wavelet transformed and inverse moment normalized to generate the watermarked image. The watermarked image is now ready for transmission. The proposed scheme can be used to validate identification cards and financial instruments. The performance of this scheme has been evaluated using a set of parameters. Experimental results show the effectiveness of this scheme.

Keywords: Image moments, wavelets, content-based watermarking, moment normalization, geometric attacks.

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Authors: M. Zarebnia, R. Parvaz

Abstract:

In this paper, numerical solutions of the nonlinear Benjamin-Bona-Mahony-Burgers (BBMB) equation are obtained by a method based on collocation of cubic B-splines. Applying the Von-Neumann stability analysis, the proposed method is shown to be unconditionally stable. The method is applied on some test examples, and the numerical results have been compared with the exact solutions. The L∞ and L2 in the solutions show the efficiency of the method computationally.

Keywords: Benjamin-Bona-Mahony-Burgers equation, Cubic Bspline, Collocation method, Finite difference.

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Authors: Zdravka Aljinović, Snježana Pivac, Boško Šego

Abstract:

According to the interaction of inflation and unemployment, expectation of the rate of inflation in Croatia is estimated. The interaction between inflation and unemployment is shown by model based on three first-order differential i.e. difference equations: Phillips relation, adaptive expectations equation and monetary-policy equation. The resulting equation is second order differential i.e. difference equation which describes the time path of inflation. The data of the rate of inflation and the rate of unemployment are used for parameters estimation. On the basis of the estimated time paths, the stability and convergence analysis is done for the rate of inflation.

Keywords: Differencing, inflation, time path, unemployment.

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Authors: Lina, Arlends Chris, Bagus Mulyawan, Agus B. Dharmawan

Abstract:

Blood cell analysis plays a significant role in the diagnosis of human health. As an alternative to the traditional technique conducted by laboratory technicians, this paper presents an automatic white blood cell (leukocyte) detection system using Image Stitching and Color Overlapping Windows. The advantage of this method is to present a detection technique of white blood cells that are robust to imperfect shapes of blood cells with various image qualities. The input for this application is images from a microscope-slide translation video. The preprocessing stage is performed by stitching the input images. First, the overlapping parts of the images are determined, then stitching and blending processes of two input images are performed. Next, the Color Overlapping Windows is performed for white blood cell detection which consists of color filtering, window candidate checking, window marking, finds window overlaps, and window cropping processes. Experimental results show that this method could achieve an average of 82.12% detection accuracy of the leukocyte images.

Keywords: Color overlapping windows, image stitching, leukocyte detection.

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Authors: Avinesh Prasad, Bibhya Sharma, Jito Vanualailai

Abstract:

This paper proposes a solution to the motion planning and control problem of a point-mass robot which is required to move safely to a designated target in a priori known workspace cluttered with fixed elliptical obstacles of arbitrary position and sizes. A tailored and unique algorithm for target convergence and obstacle avoidance is proposed that will work for any number of fixed obstacles. The control laws proposed in this paper also ensures that the equilibrium point of the given system is asymptotically stable. Computer simulations with the proposed technique and applications to a planar (RP) manipulator will be presented.

Keywords: Point-mass Robot, Asymptotic stability, Motionplanning, Planar Robot Arm.

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Authors: Kamaldeep Joshi, Rajkumar Yadav, Sachin Allwadhi

Abstract:

Image steganography is the best aspect of information hiding. In this, the information is hidden within an image and the image travels openly on the Internet. The Least Significant Bit (LSB) is one of the most popular methods of image steganography. In this method, the information bit is hidden at the LSB of the image pixel. In one bit LSB steganography method, the total numbers of the pixels and the total number of message bits are equal to each other. In this paper, the LSB method of image steganography is used for watermarking. The watermarking is an application of the steganography. The watermark contains 80*88 pixels and each pixel requirs 8 bits for its binary equivalent form so, the total number of bits required to hide the watermark are 80*88*8(56320). The experiment was performed on standard 256*256 and 512*512 size images. After the watermark insertion, histogram analysis was performed. A noise factor (salt and pepper) of 0.02 was added to the stego image in order to evaluate the robustness of the method. The watermark was successfully retrieved after insertion of noise. An experiment was performed in order to know the imperceptibility of stego and the retrieved watermark. It is clear that the LSB watermarking scheme is robust to the salt and pepper noise.

Keywords: LSB, watermarking, salt and pepper, PSNR.

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Authors: Santosh Kr. Choudhary

Abstract:

In this article, LQR based PID controller design for 3DOF helicopter system is investigated. The 3-DOF helicopter system is a benchmark laboratory model having strongly nonlinear characteristics and unstable dynamics which make the control of such system a challenging task. This article first presents the mathematical model of the 3DOF helicopter system and then illustrates the basic idea and technical formulation for controller design. The paper explains the simple approach for the approximation of PID design parameters from the LQR controller gain matrix. The simulation results show that the investigated controller has both static and dynamic performance, therefore the stability and the quick control effect can be obtained simultaneously for the 3DOF helicopter system.

Keywords: 3DOF helicopter system, PID controller, LQR controller, modeling, simulation.

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Authors: M. R. Mustafa, M. H. Isa, R. B. Rezaur

Abstract:

The use of artificial neural network (ANN) modeling for prediction and forecasting variables in water resources engineering are being increasing rapidly. Infrastructural applications of ANN in terms of selection of inputs, architecture of networks, training algorithms, and selection of training parameters in different types of neural networks used in water resources engineering have been reported. ANN modeling conducted for water resources engineering variables (river sediment and discharge) published in high impact journals since 2002 to 2011 have been examined and presented in this review. ANN is a vigorous technique to develop immense relationship between the input and output variables, and able to extract complex behavior between the water resources variables such as river sediment and discharge. It can produce robust prediction results for many of the water resources engineering problems by appropriate learning from a set of examples. It is important to have a good understanding of the input and output variables from a statistical analysis of the data before network modeling, which can facilitate to design an efficient network. An appropriate training based ANN model is able to adopt the physical understanding between the variables and may generate more effective results than conventional prediction techniques.

Keywords: ANN, discharge, modeling, prediction, sediment,

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Authors: Sebastian B. Mendes

Abstract:

The presence of a vertical edge-crack within a web plate subjected to pure bending induces local compressive stresses about the crack which may cause tension buckling. Approximate theoretical expressions were derived for the critical far-field tensile stress and bending moment capacity of an edge-cracked web plate associated with tension buckling. These expressions were validated with finite element analyses and used to investigate the possibility of tension buckling in web-cracked trial girders. It was found that tension buckling is an unlikely occurrence unless the web is relatively thin or the crack is very long.

Keywords: Fatigue crack, tension buckling, Rayleigh-Ritz, structural stability.

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Authors: Elham Amini Boroujeni, Hamid Reza Momeni

Abstract:

Design of an observer based controller for a class of fractional order systems has been done. Fractional order mathematics is used to express the system and the proposed observer. Fractional order Lyapunov theorem is used to derive the closed-loop asymptotic stability. The gains of the observer and observer based controller are derived systematically using the linear matrix inequality approach. Finally, the simulation results demonstrate validity and effectiveness of the proposed observer based controller.

Keywords: Fractional order calculus, Fractional order observer, Linear matrix inequality, Nonlinear Systems, Observer based Controller.

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Authors: C. Ardil

Abstract:

This paper proposes entropic programming for the freighter aircraft selection problem using the multiple criteria decision analysis method. The study aims to propose a systematic and comprehensive framework by focusing on the perspective of freighter aircraft selection. In order to achieve this goal, an integrated entropic programming approach was proposed to evaluate and rank alternatives. The decision criteria and aircraft alternatives were identified from the research data analysis. The objective criteria weights were determined by the mean weight method and the standard deviation method. The proposed entropic programming model was applied to a practical decision problem for evaluating and selecting freighter aircraft. The proposed entropic programming technique gives robust, reliable, and efficient results in modeling decision making analysis problems. As a result of entropic programming analysis, Boeing B747-8F, a freighter aircraft alternative ( a3), was chosen as the most suitable freighter aircraft candidate.   

Keywords: entropic programming, additive weighted model, multiple criteria decision making analysis, MCDMA, TOPSIS, aircraft selection, freighter aircraft, Boeing B747-8F, Boeing B777F, Airbus A350F

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Authors: Qamar Saeed, Vali Uddin, Reza Katebi

Abstract:

In this paper multivariable predictive PID controller has been implemented on a multi-inputs multi-outputs control problem i.e., quadruple tank system, in comparison with a simple multiloop PI controller. One of the salient feature of this system is an adjustable transmission zero which can be adjust to operate in both minimum and non-minimum phase configuration, through the flow distribution to upper and lower tanks in quadruple tank system. Stability and performance analysis has also been carried out for this highly interactive two input two output system, both in minimum and non-minimum phases. Simulations of control system revealed that better performance are obtained in predictive PID design.

Keywords: Proportional-integral-derivative Control, GeneralizedPredictive Control, Predictive PID Control, Multivariable Systems

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Authors: A. Thabet, M. Boutayeb, M. N. Abdelkrim

Abstract:

This paper investigates a method for the state estimation of nonlinear systems described by a class of differential-algebraic equation (DAE) models using the extended Kalman filter. The method involves the use of a transformation from a DAE to ordinary differential equation (ODE). A relevant dynamic power system model using decoupled techniques will be proposed. The estimation technique consists of a state estimator based on the EKF technique as well as the local stability analysis. High performances are illustrated through a simulation study applied on IEEE 13 buses test system.

Keywords: Power system, Dynamic decoupled model, Extended Kalman Filter, Convergence analysis, Time computing.

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Authors: Tirtha S. Das, Ayan K. Sau, Subir K. Sarkar

Abstract:

Digital watermarking is a way to provide the facility of secure multimedia data communication besides its copyright protection approach. The Spread Spectrum modulation principle is widely used in digital watermarking to satisfy the robustness of multimedia signals against various signal-processing operations. Several SS watermarking algorithms have been proposed for multimedia signals but very few works have discussed on the issues responsible for secure data communication and its robustness improvement. The current paper has critically analyzed few such factors namely properties of spreading codes, proper signal decomposition suitable for data embedding, security provided by the key, successive bit cancellation method applied at decoder which have greater impact on the detection reliability, secure communication of significant signal under camouflage of insignificant signals etc. Based on the analysis, robust SS watermarking scheme for secure data communication is proposed in wavelet domain and improvement in secure communication and robustness performance is reported through experimental results. The reported result also shows improvement in visual and statistical invisibility of the hidden data.

Keywords: Spread spectrum modulation, spreading code, signaldecomposition, security, successive bit cancellation

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Authors: Bu Seog Ju, Jae Sang Kim, Woo Young Jung

Abstract:

In recent year, with recent increase of interest towards leisure sports, increased number of Zip-Line or Zip-Wire facilities has built. Many researches have been actively conducted on the emphasis of the cable and the wire at the bridge. However, very limited researches have been conducted on the safety of the Zip-Line structure. In fact, fall accidents from Zip-Line have been reported frequently. Therefore, in this study, the structural safety of Zip-Line under dynamic impact loading condition were evaluated on the previously installed steel cable for leisure (Zip-Line), using 3-dimensional nonlinear Finite Element (FE) model. The result from current study would assist assurance of systematic stability of Zip-Line.

Keywords: Zip-Line, Wire, Cable, 3D FE Model, Safety.

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Authors: Sheren H. Salah, Ahmed Y. Ben Sasi

Abstract:

The inverted pendulum system is a classic control problem that is used in universities around the world. It is a suitable process to test prototype controllers due to its high non-linearities and lack of stability. The inverted pendulum represents a challenging control problem, which continually moves toward an uncontrolled state. This paper presents the possibility of balancing an inverted pendulum system using sliding mode control (SMC). The goal is to determine which control strategy delivers better performance with respect to pendulum’s angle and cart's position. Therefore, proportional-integral-derivative (PID) is used for comparison. Results have proven SMC control produced better response compared to PID control in both normal and noisy systems.

Keywords: Inverted pendulum (IP) proportional-integralderivative (PID), sliding mode control (SMC).

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Authors: M. V. Sudhamani, C. R. Venugopal

Abstract:

Content-based Image Retrieval (CBIR) aims at searching image databases for specific images that are similar to a given query image based on matching of features derived from the image content. This paper focuses on a low-dimensional color based indexing technique for achieving efficient and effective retrieval performance. In our approach, the color features are extracted using the mean shift algorithm, a robust clustering technique. Then the cluster (region) mode is used as representative of the image in 3-D color space. The feature descriptor consists of the representative color of a region and is indexed using a spatial indexing method that uses *R -tree thus avoiding the high-dimensional indexing problems associated with the traditional color histogram. Alternatively, the images in the database are clustered based on region feature similarity using Euclidian distance. Only representative (centroids) features of these clusters are indexed using *R -tree thus improving the efficiency. For similarity retrieval, each representative color in the query image or region is used independently to find regions containing that color. The results of these methods are compared. A JAVA based query engine supporting query-by- example is built to retrieve images by color.

Keywords: Content-based, indexing, cluster, region.

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Authors: Milad. A. Shalluf

Abstract:

The determination of sugars in foods is very significant. Their relation in fact, can affect the chemical and sensorial quality of the matrix (e.g., sweetness, pH, total acidity, microbial stability, global acceptability) and can provide information on food to optimize several selected technological processes. Three stages of ripeness (green, yellow and red) of tomatoes (Lycopersicon Esculentum cv. Elegance) at different harvest dates were evaluated. Fruit from all harvests were exposed to different of ozone doses (0.25, 0.50 and 1 mg O3/g tomatoes) and clean air for 5 day at 15 °C±2 and 90-95 % relative humidity. Then, fruits were submitted for extraction and analysis after a day from the finish of exposure of each stage. The concentrations of the glucose and fructose increased in the tomatoes which were subjected to ozone treatments.

Keywords: Post-harvest Treatment, Controlled Atmosphere Storage, Ozone, Tomatoes, Glucose, Fructose

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Authors: J. Cecrdle, J. Malecek

Abstract:

This paper deals with the conceptual design of the new aeroelastic demonstrator for the whirl flutter simulation. The paper gives a theoretical background of the whirl flutter phenomenon and describes the events of the whirl flutter occurrence in the aerospace practice. The second part is focused on the experimental research of the whirl flutter on aeroelastic similar models. Finally the concept of the new aeroelastic demonstrator is described. The demonstrator represents the wing and engine of the twin turboprop commuter aircraft including a driven propeller. It allows the changes of the main structural parameters influencing the whirl flutter stability characteristics. It is intended for the experimental investigation of the whirl flutter in the wind tunnel. The results will be utilized for validation of analytical methods and software tools.

Keywords: aeroelasticity, flutter, whirl flutter, W-WING demonstrator

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Authors: S. Heydarian

Abstract:

Proton transfer and hydrogen bonding are two aspects of the chemistry of hydrogen that respectively govern the behaviour and structure of many molecules, both simple and complex. All the theoretical enol and keto conformations of 1,3-diphenyl-1,3- propandion known as dibenzoylmethane (DBM), have been investigated by means of atoms in molecules (AIM) theory. It was found that the most stable conformers are those stabilized by hydrogen bridges.The aim of the present paper is a thorough conformational analysis of DBM (with special attention on chelated cis-enol conformers) in order to obtain detailed information on the geometrical parameters, relative stabilities and rotational motion of the phenyl groups. It is also important to estimate the barrier height for ptoton transfer and hydrogen bond strength, which are the main factors governing conformational stability.

Keywords: Acetylacetone, Atoms in molecules, Dibenzoylmethane, Intramolecular hydrogen bond, Resonanceconjugation

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Authors: H. Bouadi, M. Bouchoucha, M. Tadjine

Abstract:

In this paper; we are interested principally in dynamic modelling of quadrotor while taking into account the high-order nonholonomic constraints in order to develop a new control scheme as well as the various physical phenomena, which can influence the dynamics of a flying structure. These permit us to introduce a new state-space representation. After, the use of Backstepping approach for the synthesis of tracking errors and Lyapunov functions, a sliding mode controller is developed in order to ensure Lyapunov stability, the handling of all system nonlinearities and desired tracking trajectories. Finally simulation results are also provided in order to illustrate the performances of the proposed controller.

Keywords: Dynamic modeling, nonholonomic constraints, Backstepping, sliding mode.

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Authors: Vinod Kumar, Navin Kumar, Surjit Angra, Prince Sharma

Abstract:

This paper presents the design analysis of saddle support of a horizontal pressure vessel. Since saddle have the vital role to support the pressure vessel and to maintain its stability, it should be designed in such a way that it can afford the vessel load and internal pressure of the vessel due to liquid contained in the vessel. A model of horizontal pressure vessel and saddle support is created in ANSYS. Stresses are calculated using mathematical approach and ANSYS software. The analysis reveals the zone of high localized stress at the junction part of the pressure vessel and saddle support due to operating conditions. The results obtained by both the methods are compared with allowable stress value for safe designing.

Keywords: ANSYS, Pressure Vessel, Saddle, Support.

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Authors: Jaedo Kim, Youngjoon Han, Hernsoo Hahn

Abstract:

This paper propose the robust character segmentation method for license plate with topological transform such as twist,rotation. The first step of the proposed method is to find a candidate region for character and license plate. The character or license plate must be appeared as closed loop in the edge image. In the case of detecting candidate for character region, the evaluation of detected region is using topological relationship between each character. When this method decides license plate candidate region, character features in the region with binarization are used. After binarization for the detected candidate region, each character region is decided again. In this step, each character region is fitted more than previous step. In the next step, the method checks other character regions with different scale near the detected character regions, because most license plates have license numbers with some meaningful characters around them. The method uses perspective projection for geometrical normalization. If there is topological distortion in the character region, the method projects the region on a template which is defined as standard license plate using perspective projection. In this step, the method is able to separate each number region and small meaningful characters. The evaluation results are tested with a number of test images.

Keywords: License Plate Detection, Character Segmentation, Perspective Projection, Topological Transform.

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Authors: Yi-Haur Shiau, Sun-In Lin, Shi-Wei Lo, Hsiu-Mei Chou, Yi-Hsuan Chen

Abstract:

This paper proposes a novel multi-format stream grid architecture for real-time image monitoring system. The system, based on a three-tier architecture, includes stream receiving unit, stream processor unit, and presentation unit. It is a distributed computing and a loose coupling architecture. The benefit is the amount of required servers can be adjusted depending on the loading of the image monitoring system. The stream receive unit supports multi capture source devices and multi-format stream compress encoder. Stream processor unit includes three modules; they are stream clipping module, image processing module and image management module. Presentation unit can display image data on several different platforms. We verified the proposed grid architecture with an actual test of image monitoring. We used a fast image matching method with the adjustable parameters for different monitoring situations. Background subtraction method is also implemented in the system. Experimental results showed that the proposed architecture is robust, adaptive, and powerful in the image monitoring system.

Keywords: Motion detection, grid architecture, image monitoring system, and background subtraction.

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Authors: Xuan Li, Weian Huang, Jinsheng Sun, Fuhao Zhao, Zhiyuan Wang, Jintang Wang

Abstract:

Natural gas hydrates (NGHs) as promising alternative energy sources have gained increasing attention. Hydrate-bearing formation in marine areas is highly unconsolidated formation and is fragile, which is composed of weakly cemented sand-clay and silty sediments. During the drilling process, the invasion of drilling fluid can easily lead to excessive water content in the formation. It will change the soil liquid plastic limit index, which significantly affects the formation quality, leading to wellbore instability due to the metastable character of hydrate-bearing sediments. Therefore, controlling the filtrate loss into the formation in the drilling process has to be highly regarded for protecting the stability of the wellbore. In this study, the temperature-sensitive nanogel of P(NIPAM-co-AMPS-co-tBA) was prepared by soap-free emulsion polymerization, and the temperature-sensitive behavior was employed to achieve self-adaptive plugging in hydrate sediments. First, the effects of additional amounts of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), tert-butyl acrylate (tBA), and methylene-bis-acrylamide (MBA) on the microgel synthesis process and temperature-sensitive behaviors were investigated. Results showed that, as a reactive emulsifier, AMPS can not only participate in the polymerization reaction but also act as an emulsifier to stabilize micelles and enhance the stability of nanoparticles. The volume phase transition temperature (VPTT) of nanogels gradually decreased with the increase of the contents of hydrophobic monomer tBA. An increase in the content of the cross-linking agent MBA can lead to a rise in the coagulum content and instability of the emulsion. The plugging performance of nanogel was evaluated in a core sample with a pore size distribution range of 100-1000 nm. The temperature-sensitive nanogel can effectively improve the microfiltration performance of drilling fluid. Since a combination of a series of nanogels could have a wide particle size distribution at any temperature, around 200 nm to 800 nm, the self-adaptive plugging capacity of nanogels for the hydrate sediments was revealed. Thermosensitive nanogel is a potential intelligent plugging material for drilling operations in NGH-bearing sediments.

Keywords: Temperature-sensitive nanogel, NIPAM, self-adaptive plugging performance, drilling operations, hydrate-bearing sediments.

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Authors: Yoshihiro Ochiai

Abstract:

To unveil the mechanism of fast autooxidation of fish myoglobins, the effect of temperature on the structural change of tuna myoglobin was investigated. Purified myoglobin was subjected to preincubation at 5, 20, 50 and 40oC. Overall helical structural decay through thermal treatment up to 95oC was monitored by circular dichroism spectrometry, while the structural changes around the heme pocket was measured by ultraviolet/visible absorption spectrophotometry. As a result, no essential structural change of myoglobin was observed under 30oC, roughly equivalent to their body temperature, but the structure was clearly damaged at 40oC. The Soret band absorption hardly differed irrespective of preincubation temperature, suggesting that the structure around the heme pocket was not perturbed even after thermal treatment.

Keywords: denaturation, myoglobin, stability, tuna.

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