Search results for: laser transmission
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1132

Search results for: laser transmission

502 Application of Hermite-Rodriguez Functions to Pulse Shaping Analog Filter Design

Authors: Mohd Amaluddin Yusoff

Abstract:

In this paper, we consider the design of pulse shaping filter using orthogonal Hermite-Rodriguez basis functions. The pulse shaping filter design problem has been formulated and solved as a quadratic programming problem with linear inequality constraints. Compared with the existing approaches reported in the literature, the use of Hermite-Rodriguez functions offers an effective alternative to solve the constrained filter synthesis problem. This is demonstrated through a numerical example which is concerned with the design of an equalization filter for a digital transmission channel.

Keywords: channel equalization filter, Hermite-Rodriguez, pulseshaping filter, quadratic programming.

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501 A Novel Transmission Scheme for Reliable Cooperative Communication

Authors: Won-Jun Choi, Seung-Jun Yu, Jung-In Baik, Hyoung-Kyu Song

Abstract:

Cooperative communication scheme can be substituted for multiple-input multiple-output (MIMO) technique when it may not be able to support multiple antennas due to size, cost or hardware limitations. In other words, cooperative communication scheme is an efficient method to achieve spatial diversity without multiple antennas. For satisfaction of rising QoS, we propose a reliable cooperative communication scheme with M-QAM based Dual Carrier Modulation (M-DCM), which can increase diversity gain. Although our proposed scheme is very simple method, it gives us frequency and spatial diversity. Simulation result shows our proposed scheme obtains diversity gain more than the conventional cooperative communication scheme.

Keywords: cooperation, diversity, M-DCM, OFDM.

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500 Shadow Imaging Study of Z-Pinch Dynamic Hohlraum

Authors: Chen Faxin, Feng Jinghua, Yang Jianlun, Li Linbo, Zhou Lin

Abstract:

In order to obtaining the dynamic evolution image of Tungsten array for foam padding, and to research the form of interaction between Tungsten plasma and foam column, a shadow imaging system of four-frame ultraviolet probe laser (266nm)has been designed on 1MA pulse power device. The time resolution of the system is 2.5ns, and static space resolution is superior to 70μm. The radial shadowgraphy image reveals the whole process from the melting and expansion of solid wire to the interaction of the precursor plasma and the foam, from the pinch to rebound inflation. The image shows the continuous interaction of Tungsten plasma and foam in a form of “Raining" within a time of about 50ns, the plasma shell structure has not been found in the whole period of pinch. The quantitative analysis indicates the minimum pinching speed of the foam column is 1.0×106cm/s, and maximum pinching speed is 6.0×106cm/s, and the axial stagnation diameter is approx 1mm.

Keywords: Dynamic hohlraum, Shadowgraphy image, Foam evolution.

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499 Adaptive Subchannel Allocation for MC-CDMA System

Authors: Cuiran Li, Jianli Xie, Chengshu Li

Abstract:

Multicarrier code-division multiple-access is one of the effective techniques to gain its multiple access capability, robustness against fading, and to mitigate the ISI. In this paper, we propose an improved mulcarrier CDMA system with adaptive subchannel allocation. We analyzed the performance of our proposed system in frequency selective fading environment with narrowband interference existing and compared it with that of parallel transmission over many subchannels (namely, conventional MC-CDMA scheme) and DS-CDMA system. Simulation results show that adaptive subchannel allocation scheme, when used in conventional multicarrier CDMA system, the performance will be greatly improved.

Keywords: MC-CDMA, Rayleigh fading, Narrowbandinterference, Channel estimation.

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498 Database Compression for Intelligent On-board Vehicle Controllers

Authors: Ágoston Winkler, Sándor Juhász, Zoltán Benedek

Abstract:

The vehicle fleet of public transportation companies is often equipped with intelligent on-board passenger information systems. A frequently used but time and labor-intensive way for keeping the on-board controllers up-to-date is the manual update using different memory cards (e.g. flash cards) or portable computers. This paper describes a compression algorithm that enables data transmission using low bandwidth wireless radio networks (e.g. GPRS) by minimizing the amount of data traffic. In typical cases it reaches a compression rate of an order of magnitude better than that of the general purpose compressors. Compressed data can be easily expanded by the low-performance controllers, too.

Keywords: Data analysis, data compression, differentialencoding, run-length encoding, vehicle control.

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497 On the Fast Convergence of DD-LMS DFE Using a Good Strategy Initialization

Authors: Y.Ben Jemaa, M.Jaidane

Abstract:

In wireless communication system, a Decision Feedback Equalizer (DFE) to cancel the intersymbol interference (ISI) is required. In this paper, an exact convergence analysis of the (DFE) adapted by the Least Mean Square (LMS) algorithm during the training phase is derived by taking into account the finite alphabet context of data transmission. This allows us to determine the shortest training sequence that allows to reach a given Mean Square Error (MSE). With the intention of avoiding the problem of ill-convergence, the paper proposes an initialization strategy for the blind decision directed (DD) algorithm. This then yields a semi-blind DFE with high speed and good convergence.

Keywords: Adaptive Decision Feedback Equalizer, PerformanceAnalysis, Finite Alphabet Case, Ill-Convergence, Convergence speed.

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496 Effect of Atmospheric Turbulence on AcquisitionTime of Ground to Deep Space Optical Communication System

Authors: Hemani Kaushal, V.K.Jain, Subrat Kar

Abstract:

The performance of ground to deep space optical communication systems is degraded by distortion of the beam as it propagates through the turbulent atmosphere. Turbulence causes fluctuations in the intensity of the received signal which ultimately affects the acquisition time required to acquire and locate the spaceborne target using narrow laser beam. In this paper, performance of free-space optical (FSO) communication system in atmospheric turbulence has been analyzed in terms of acquisition time for coherent and non-coherent modulation schemes. Numerical results presented in graphical and tabular forms show that the acquisition time increases with the increase in turbulence level. This is true for both schemes. The BPSK has lowest acquisition time among all schemes. In non-coherent schemes, M-PPM performs better than the other schemes. With the increase in M, acquisition time becomes lower, but at the cost of increase in system complexity.

Keywords: Atmospheric Turbulence, Acquisition Time, BinaryPhase Shift Keying (BPSK), Free-Space Optical (FSO)Communication System, M-ary Pulse Position Modulation (M-PPM), Coherent/Non-coherent Modulation Schemes.

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495 Characterising the Effects of Sand Blasting on Formed Steel Samples

Authors: Esther T. Akinlabi, Enoch Ogunmuyiwa, Stephen A. Akinlabi

Abstract:

The present research study focuses on the investigation of the influence of sand blasting on formed mild steel samples. The investigation involved the examinations on the parent material and a sand blasted material. The results were compared to the mechanically formed materials (sand and non-sand blasted) as well as a laser formed material (sand and non-sand blasted). Each material was characterized for the grain sizes and hardness. The percentage change in the grain sizes was quantified and correlation to the microhardness values was established. The Ultimate Tensile Strength (UTS) of the materials was also quantified using the obtained hardness values. The investigations revealed that the sand blasting causes an increase in the Vickers microhardness values of all the materials which also led to an increase in the UTS. After the forming operation, the microstructure revealed elongated grains as compared to almost equiaxed obtained from the parent non-sand blasted materials.

Keywords: Grain size, hardness, metal forming, sand blasting, ultimate tensile strength.

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494 Code-Aided Turbo Channel Estimation for OFDM Systems with NB-LDPC Codes

Authors: Ł. Januszkiewicz, G. Bacci, H. Gierszal, M. Luise

Abstract:

In this paper channel estimation techniques are considered as the support methods for OFDM transmission systems based on Non Binary LDPC (Low Density Parity Check) codes. Standard frequency domain pilot aided LS (Least Squares) and LMMSE (Linear Minimum Mean Square Error) estimators are investigated. Furthermore, an iterative algorithm is proposed as a solution exploiting the NB-LDPC channel decoder to improve the performance of the LMMSE estimator. Simulation results of signals transmitted through fading mobile channels are presented to compare the performance of the proposed channel estimators.

Keywords: LDPC codes, LMMSE, OFDM, turbo channelestimation.

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493 Adaptive Radio Resource Allocation for Multiple Traffic OFDMA Broadband Wireless Access System

Authors: Lu Yanhui, Zhang Lizhi, Yin Changchuan, Yue Guangxin

Abstract:

In this paper, an adaptive radio resource allocation (RRA) algorithm applying to multiple traffic OFDMA system is proposed, which distributes sub-carrier and loading bits among users according to their different QoS requirements and traffic class. By classifying and prioritizing the users based on their traffic characteristic and ensuring resource for higher priority users, the scheme decreases tremendously the outage probability of the users requiring a real time transmission without impact on the spectrum efficiency of system, as well as the outage probability of data users is not increased compared with the RRA methods published.

Keywords: OFDMA, adaptive radio resource allocation, QoS.

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492 Analysis of Time Delay Simulation in Networked Control System

Authors: Nyan Phyo Aung, Zaw Min Naing, Hla Myo Tun

Abstract:

The paper presents a PD controller for the Networked Control Systems (NCS) with delay. The major challenges in this networked control system (NCS) are the delay of the data transmission throughout the communication network. The comparative performance analysis is carried out for different delays network medium. In this paper, simulation is carried out on Ac servo motor control system using CAN Bus as communication network medium. The True Time toolbox of MATLAB is used for simulation to analyze the effect of different delays.

Keywords: NCS, Time delay, CAN Bus, True time, MATLAB.

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491 Preparation and Physical Characterization of Nanocomposites of PLA / Layered Silicates

Authors: I. Restrepo, S. Solorzano

Abstract:

This work was focused in to study the compatibility, dispersion and exfoliation of modified nanoclays in biodegradable polymers and evaluate its effect on the physical, mechanical and thermal properties on the biodegradable matrix used. The formulations have been developed with polylactic acid (PLA) and organically modified montmorillonite-type commercial nanoclays (Cloisite 15, Cloisite 20, and Cloisite 30B) in the presence of a plasticizer agent, specifically Polyethylene Glycol of low molecular weight. Different compositions were evaluated, in order to identify the influence of each nanoclayin the polymeric matrix. The mixtures were characterized by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (DRX), transmission electron microscopy (TEM) and Tensile Test. These tests have allowed understanding the behavior of each of the mixtures developed.

Keywords: Biopolymers, Nanoclays, polylacticacid (PLA), polymer blends.

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490 Examining the Modular End of Line Control Unit Design Criteria for Vehicle Sliding Door System Track Profile

Authors: O. Kurtulus, C. Yavuz

Abstract:

The end of the line controls of the finished products in the automotive industry is important. The control that has been conducted with the manual methods for the sliding doors tracks is not sufficient and faulty products cannot be identified. As a result, the customer has the faulty products. In the scope of this study, the design criteria of the PLC integrated modular end of line control unit has been examined, designed and manufactured to make the control of the 10 different track profile to 2 different vehicles with an objective to minimize the salvage costs by obtaining more sensitive, certain and accurate measurement results. In the study that started with literature and patent review, the design inputs have been specified, the technical concept has been developed, computer supported mechanic design, control system and automation design, design review and design improvement have been made. Laser analog sensors at high sensitivity, probes and modular blocks have been used in the unit. The measurement has been conducted in the system and it is observed that measurement results are more sensitive than the previous methods that we use.

Keywords: Control unit design, end of line, modular design, sliding door system.

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489 Vulnerabilities of IEEE 802.11i Wireless LAN CCMP Protocol

Authors: M. Junaid , Muid Mufti, M. Umar Ilyas

Abstract:

IEEE has recently incorporated CCMP protocol to provide robust security to IEEE 802.11 wireless LANs. It is found that CCMP has been designed with a weak nonce construction and transmission mechanism, which leads to the exposure of initial counter value. This weak construction of nonce renders the protocol vulnerable to attacks by intruders. This paper presents how the initial counter can be pre-computed by the intruder. This vulnerability of counter block value leads to pre-computation attack on the counter mode encryption of CCMP. The failure of the counter mode will result in the collapse of the whole security mechanism of 802.11 WLAN.

Keywords: Information Security, Cryptography, IEEE 802.11i, Computer security, Wireless LAN

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488 Plasmonic Absorption Enhancement in Au/CdS Nanocomposite

Authors: K. Easawi, M. Nabil, T. Abdallah, S. Negm, H. Talaat

Abstract:

Composite nanostructures of metal core/semiconductor shell (Au/CdS) configuration were prepared using organometalic method. UV-Vis spectra for the Au/CdS colloids show initially two well separated bands, corresponding to surface plasmon of the Au core, and the exciton of CdS shell. The absorption of CdS shell is enhanced, while the Au plasmon band is suppressed as the shell thickness increases. The shell sizes were estimated from the optical spectra using the effective mass approximation model (EMA), and compared to the sizes of the Au core and CdS shell measured by high resolution transmission electron microscope (HRTEM). The changes in the absorption features are discussed in terms of gradual increase in the coupling strength of the Au core surface plasmon and the exciton in the CdS. leading to charge transfer and modification of electron oscillation in Au core.

Keywords: Nanocomposites, Plasmonics.

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487 Power Line Carrier for Power Telemetering

Authors: Tosaphol Ratniyomchai, Uthai Jaithong, Thanatchai Kulworawanichpong

Abstract:

This paper presents an application of power line carrier (PLC) for electrical power telemetering. This system has a special capability of transmitting the measured values to a centralized computer via power lines. The PLC modem as a passive high-pass filter is designed for transmitting and receiving information. Its function is to send the information carrier together with transmitted data by superimposing it on the 50 Hz power frequency signal. A microcontroller is employed to function as the main processing of the modem. It is programmed for PLC control and interfacing with other devices. Each power meter, connected via a PLC modem, is assigned with a unique identification number (address) for distinguishing each device from one another.

Keywords: Power telemetering, Power line carrier, High-passfilter, Digital data transmission

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486 Multivariable Predictive PID Control for Quadruple Tank

Authors: Qamar Saeed, Vali Uddin, Reza Katebi

Abstract:

In this paper multivariable predictive PID controller has been implemented on a multi-inputs multi-outputs control problem i.e., quadruple tank system, in comparison with a simple multiloop PI controller. One of the salient feature of this system is an adjustable transmission zero which can be adjust to operate in both minimum and non-minimum phase configuration, through the flow distribution to upper and lower tanks in quadruple tank system. Stability and performance analysis has also been carried out for this highly interactive two input two output system, both in minimum and non-minimum phases. Simulations of control system revealed that better performance are obtained in predictive PID design.

Keywords: Proportional-integral-derivative Control, GeneralizedPredictive Control, Predictive PID Control, Multivariable Systems

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485 Access Control System: Monitoring Tool for Fiber to the Home Passive Optical Network

Authors: Aswir Premadi, Mohammad Syuhaimi Ab. Rahman, Mohamad Najib Moh. Saupe, KasmiranJumari

Abstract:

An optical fault monitoring in FTTH-PON using ACS is demonstrated. This device can achieve real-time fault monitoring for protection feeder fiber. In addition, the ACS can distinguish optical fiber fault from the transmission services to other customers in the FTTH-PON. It is essential to use a wavelength different from the triple-play services operating wavelengths for failure detection. ACS is using the operating wavelength 1625 nm for monitoring and failure detection control. Our solution works on a standard local area network (LAN) using a specially designed hardware interfaced with a microcontroller integrated Ethernet.

Keywords: ACS, monitoring tool, FTTH-PON.

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484 Structural, Optical and Ferroelectric Properties of BaTiO3 Sintered at Different Temperatures

Authors: Anurag Gaur, Neha Sharma

Abstract:

In this work, we have synthesized BaTiO3 via sol gel method by sintering at different temperatures (600, 700, 800, 900, 10000C) and studied their structural, optical and ferroelectric properties through X-ray diffraction (XRD), UV-Vis spectrophotometer and PE Loop Tracer. X-ray diffraction patterns of barium titanate samples show that the peaks of the diffractogram are successfully indexed with the tetragonal and cubic structure of BaTiO3. The Optical band gap calculated through UV Visible spectrophotometer varies from 4.37 to 3.80 eV for the samples sintered at 600 to 10000C, respectively. The particle size calculated through transmission electron microscopy varies from 20 to 40 nm for the samples sintered at 600 to 10000C, respectively. Moreover, it has been observed that the ferroelectricity increases as we increase the sintering temperature.

Keywords: Nanostructures, Ferroelectricity, Sol-gel method.

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483 A Low Noise Microwave Filter with Minimum Distortion

Authors: Cheng Yuan Hung, Min Hang Weng, Siang Wen Lan, Wei Yu Chen, Hung Wei Wu, Chun Yueh Huang

Abstract:

In this paper, a low noise microwave bandpass filter (BPF) is presented. This filter is fabricated by modifying the conventional cross-coupled structure. The spurious response is improved by using the end open coupled lines, and the influence of the noise is minimized. Impedance matrix of the open end coupled circuit clarifies the characteristic of the suppression of the spurious response. The rejection of spurious suppression region of the proposed filter is greater than 20 dB from 3-13 GHz. The measured results of the fabricated filter confirm the concepts of the proposed design and exhibits high performance.

Keywords: Low noise, signal transmission, bandpass filter, end open coupled line, communication system.

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482 New Multipath Node-Disjoint Routing Based on AODV Protocol

Authors: V. Zangeneh, S. Mohammadi

Abstract:

Today, node-disjoint routing becomes inessential technique in communication of packets among various nodes in networks. Meanwhile AODV (Ad Hoc On-demand Multipath Distance Vector) creates single-path route between a pair of source and destination nodes. Some researches has done so far to make multipath node-disjoint routing based on AODV protocol. But however their overhead and end-to-end delay are relatively high, while the detail of their code is not available too. This paper proposes a new approach of multipath node-disjoint routing based on AODV protocol. Then the algorithm of analytical model is presented. The extensive results of this algorithm will be presented in the next paper.

Keywords: AODV; MANET; Multipath Routing; Node-disjoint;transmission delay.

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481 Comparison of SVC and STATCOM in Static Voltage Stability Margin Enhancement

Authors: Mehrdad Ahmadi Kamarposhti, Mostafa Alinezhad

Abstract:

One of the major causes of voltage instability is the reactive power limit of the system. Improving the system's reactive power handling capacity via Flexible AC transmission System (FACTS) devices is a remedy for prevention of voltage instability and hence voltage collapse. In this paper, the effects of SVC and STATCOM in Static Voltage Stability Margin Enhancement will be studied. AC and DC representations of SVC and STATCOM are used in the continuation power flow process in static voltage stability study. The IEEE-14 bus system is simulated to test the increasing loadability. It is found that these controllers significantly increase the loadability margin of power systems.

Keywords: SVC, STATCOM, Voltage Collapse, Maximum Loading Point.

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480 Adaptive Routing Protocol for Dynamic Wireless Sensor Networks

Authors: Fayez Mostafa Alhamoui, Adnan Hadi Mahdi Al- Helali

Abstract:

The main issue in designing a wireless sensor network (WSN) is the finding of a proper routing protocol that complies with the several requirements of high reliability, short latency, scalability, low power consumption, and many others. This paper proposes a novel routing algorithm that complies with these design requirements. The new routing protocol divides the WSN into several subnetworks and each sub-network is divided into several clusters. This division is designed to reduce the number of radio transmission and hence decreases the power consumption. The network division may be changed dynamically to adapt with the network changes and allows the realization of the design requirements.

Keywords: Wireless sensor networks, routing protocols, ad hoc topology, cluster, sub-network, WSN design requirements.

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479 Detection of Breast Cancer in the JPEG2000 Domain

Authors: Fayez M. Idris, Nehal I. AlZubaidi

Abstract:

Breast cancer detection techniques have been reported to aid radiologists in analyzing mammograms. We note that most techniques are performed on uncompressed digital mammograms. Mammogram images are huge in size necessitating the use of compression to reduce storage/transmission requirements. In this paper, we present an algorithm for the detection of microcalcifications in the JPEG2000 domain. The algorithm is based on the statistical properties of the wavelet transform that the JPEG2000 coder employs. Simulation results were carried out at different compression ratios. The sensitivity of this algorithm ranges from 92% with a false positive rate of 4.7 down to 66% with a false positive rate of 2.1 using lossless compression and lossy compression at a compression ratio of 100:1, respectively.

Keywords: Breast cancer, JPEG2000, mammography, microcalcifications.

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478 A Development of Personalized Edutainment Contents through Storytelling

Authors: Min Kyeong Cha, Ju Yeon Mun, Seong Baeg Kim

Abstract:

Recently, ‘play of learning’ becomes important and is  emphasized as a useful learning tool. Therefore, interest in  edutainment contents is growing. Storytelling is considered first as a  method that improves the transmission of information and learner's  interest when planning edutainment contents. In this study, we  designed edutainment contents in the form of an adventure game that  applies the storytelling method. This content provides questions and  items constituted dynamically and reorganized learning contents  through analysis of test results. It allows learners to solve various  questions through effective iterative learning. As a result, the learners  can reach mastery learning.

 

Keywords: Storytelling, edutainment, mastery learning, computer operating principle.

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477 Optical Wireless Sensor Networks Based on VLC with PLC-Ethernet Interface

Authors: Heejin Lee, Yeonjoo Kim, Kyungrak Sohn

Abstract:

We present a white LED-based optical wireless communication systems for indoor ubiquitous sensor networks. Each sensor node could access to the server through the PLC (Power Line Communication)-Ethernet interface. The proposed system offers a full-duplex wireless link by using different wavelengths to reduce the inter-symbol interference between uplink and downlink. Through the 1-to-n optical wireless sensor network and PLC modem, the mobile terminals send a temperature data to server. The data transmission speed and distance are 115.2kbps and about 60cm, respectively.

Keywords: Visible light communications, LED lighting, powerline communications, ubiquitous sensor networks, full-duplex links

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476 The Libyc Writing

Authors: S. Ait Ali Yahia

Abstract:

One of the main features of the Maghreb is its linguistic richness. The multilingualism is a fact which always marked the Maghreb since the beginning of the history up to know. Since the arrival of the Phoenicians, followed by the Carthaginians, Romans, and Arabs, etc, there was a social group in the Maghreb which controlled two kinds of idioms. The libyc one remained, despite everything, the local language used by the major part of the population. This language had a support of written transmission attested by many inscriptions. Among all the forms of the Maghreb writing, this alphabet, however, continues to cause a certain number of questions about the origin and the date of its appearance. The archaeological, linguistic and historical data remain insufficient to answer these questions. This did not prevent the researchers from giving an opinion. In order to answer these questions we will expose here the various assumptions adopted by various authors who are founded on more or less explicit arguments. We will also speak about the various forms taken by the libyc writing during antiquity.

Keywords: The alphabet libyc, Eastern libyc, Western libyc.

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475 Modeling of a Second Order Non-Ideal Sigma-Delta Modulator

Authors: Abdelghani Dendouga, Nour-Eddine Bouguechal, Souhil Kouda, Samir Barra

Abstract:

A behavioral model of a second order switchedcapacitor Sigma-Delta modulator is presented. The purpose of this work is the presentation of a behavioral model of a second order switched capacitor ΣΔ modulator considering (Error due to Clock Jitter, Thermal noise Amplifier Noise, Amplifier Slew-Rate, Non linearity of amplifiers, Gain error, Charge Injection, Clock Feedthrough, and Nonlinear on-resistance). A comparison between the use of MOS switches and the use transmission gate switches use is analyzed.

Keywords: Charge injection, clock feed through, Sigma Deltamodulators, Sigma Delta non-idealities, switched capacitor.

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474 An Ultra-Low Output Impedance Power Amplifier for Tx Array in 7-Tesla Magnetic Resonance Imaging

Authors: Ashraf Abuelhaija, Klaus Solbach

Abstract:

In Ultra high-field MRI scanners (3T and higher), parallel RF transmission techniques using multiple RF chains with multiple transmit elements are a promising approach to overcome the high-field MRI challenges in terms of inhomogeneity in the RF magnetic field and SAR. However, mutual coupling between the transmit array elements disturbs the desirable independent control of the RF waveforms for each element. This contribution demonstrates a 18 dB improvement of decoupling (isolation) performance due to the very low output impedance of our 1 kW power amplifier.

Keywords: EM coupling, Inter-element isolation, Magnetic resonance imaging (MRI), Parallel Transmit.

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473 Phenotypical and Genotypical Assessment Techniques for Identification of Some Contagious Mastitis Pathogens

Authors: A. El Behiry, R. N. Zahran, R. Tarabees, E. Marzouk, M. Al-Dubaib

Abstract:

Mastitis is one of the most economic disease affecting dairy cows worldwide. Its classic diagnosis using bacterial culture and biochemical findings is a difficult and prolonged method. In this research, using of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) permitted identification of different microorganisms with high accuracy and rapidity (only 24 hours for microbial growth and analysis). During the application of MALDI-TOF MS, one hundred twenty strains of Staphylococcus and Streptococcus species isolated from milk of cows affected by clinical and subclinical mastitis were identified, and the results were compared with those obtained by traditional methods as API and VITEK 2 Systems. 37 of totality 39 strains (~95%) of Staphylococcus aureus (S. aureus) were exactly detected by MALDI TOF MS and then confirmed by a nuc-based PCR technique, whereas accurate identification was observed in 100% (50 isolates) of the coagulase negative staphylococci (CNS) and Streptococcus agalactiae (31 isolates). In brief, our results demonstrated that MALDI-TOF MS is a fast and truthful technique which has the capability to replace conventional identification of several bacterial strains usually isolated in clinical laboratories of microbiology.

Keywords: Identification, mastitis pathogens, mass spectral, phenotypical.

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