Search results for: food process control
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 9005

Search results for: food process control

3395 Multilevel Arnoldi-Tikhonov Regularization Methods for Large-Scale Linear Ill-Posed Systems

Authors: Yiqin Lin, Liang Bao

Abstract:

This paper is devoted to the numerical solution of large-scale linear ill-posed systems. A multilevel regularization method is proposed. This method is based on a synthesis of the Arnoldi-Tikhonov regularization technique and the multilevel technique. We show that if the Arnoldi-Tikhonov method is a regularization method, then the multilevel method is also a regularization one. Numerical experiments presented in this paper illustrate the effectiveness of the proposed method.

Keywords: Discrete ill-posed problem, Tikhonov regularization, discrepancy principle, Arnoldi process, multilevel method.

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3394 Performance of an Electrocoagulation Process in Treating Direct Dye: Batch and Continuous Upflow Processes

Authors: C. Phalakornkule, S. Polgumhang, W. Tongdaung

Abstract:

This study presents an investigation of electrochemical variables and an application of the optimal parameters in operating a continuous upflow electrocoagulation reactor in removing dye. Direct red 23, which is azo-based, was used as a representative of direct dyes. First, a batch mode was employed to optimize the design parameters: electrode type, electrode distance, current density and electrocoagulation time. The optimal parameters were found to be iron anode, distance between electrodes of 8 mm and current density of 30 A·m-2 with contact time of 5 min. The performance of the continuous upflow reactor with these parameters was satisfactory, with >95% color removal and energy consumption in the order of 0.6-0.7 kWh·m-3.

Keywords: Decolorization, Direct Dye, Electrocoagulation, Textile Wastewater, Upflow Reactor.

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3393 A Preliminary Technology Assessment Analysis for the use of High Pressure Treatment on Halloumi Cheese

Authors: Michalis Menicou, Stavros Christofi, Niki Chartosia, Vassos Vassiliou, Marios Charalambides

Abstract:

This paper presents preliminary results of a technology assessment analysis for the use of high pressure treatment (HPT) on Halloumi cheese. In particular, it presents the importance of this traditional Cyprus cheese to the island-s economy, explains its production process, and gives a brief introduction to HPT and its application on cheese. More importantly, it offers preliminary results of HPT of Halloumi samples and a preliminary economic feasibility study on the financial implications of the introduction of such technology.

Keywords: Economic feasibility analysis, high pressure treatment, Halloumi cheese, technology assessment

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3392 A New Approach for Network Reconfiguration Problem in Order to Deviation Bus Voltage Minimization with Regard to Probabilistic Load Model and DGs

Authors: Mahmood Reza Shakarami, Reza Sedaghati

Abstract:

Recently, distributed generation technologies have received much attention for the potential energy savings and reliability assurances that might be achieved as a result of their widespread adoption. The distribution feeder reconfiguration (DFR) is one of the most important control schemes in the distribution networks, which can be affected by DGs. This paper presents a new approach to DFR at the distribution networks considering wind turbines. The main objective of the DFR is to minimize the deviation of the bus voltage. Since the DFR is a nonlinear optimization problem, we apply the Adaptive Modified Firefly Optimization (AMFO) approach to solve it. As a result of the conflicting behavior of the single- objective function, a fuzzy based clustering technique is employed to reach the set of optimal solutions called Pareto solutions. The approach is tested on the IEEE 32-bus standard test system.

Keywords: Adaptive Modified Firefly Optimization (AMFO), Pareto solutions, feeder reconfiguration, wind turbines, bus voltage.

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3391 A Single Phase ZVT-ZCT Power Factor Correction Boost Converter

Authors: Yakup Sahin, Naim Suleyman Ting, Ismail Aksoy

Abstract:

In this paper, a single phase soft switched Zero Voltage Transition and Zero Current Transition (ZVT-ZCT) Power Factor Correction (PFC) boost converter is proposed. In the proposed PFC converter, the main switch turns on with ZVT and turns off with ZCT without any additional voltage or current stresses. Auxiliary switch turns on and off with zero current switching (ZCS). Also, the main diode turns on with zero voltage switching (ZVS) and turns off with ZCS. The proposed converter has features like low cost, simple control and structure. The output current and voltage are controlled by the proposed PFC converter in wide line and load range. The theoretical analysis of converter is clarified and the operating steps are given in detail. The simulation results of converter are obtained for 500 W and 100 kHz. It is observed that the semiconductor devices operate with soft switching (SS) perfectly. So, the switching power losses are minimum. Also, the proposed converter has 0.99 power factor with sinusoidal current shape.

Keywords: Power factor correction, zero-voltage transition, zero-current transition, soft switching.

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3390 Extractability of Heavy Metals in Green Liquor Dregs using Artificial Sweat and Gastric Fluids

Authors: Kati Manskinen, Risto Pöykiö, Hannu Nurmesniemi

Abstract:

In an assessment of the extractability of metals in green liquor dregs from the chemical recovery circuit of semichemical pulp mill, extractable concentrations of heavy metals in artificial gastric fluid were between 10 (Ni) and 717 (Zn) times higher than those in artificial sweat fluid. Only Al (6.7 mg/kg; d.w.), Ni (1.2 mg/kg; d.w.) and Zn (1.8 mg/kg; d.w.) showed extractability in the artificial sweat fluid, whereas Al (730 mg/kg; d.w.), Ba (770 mg/kg; d.w.) and Zn (1290 mg/kg; d.w.) showed clear extractability in the artificial gastric fluid. As certain heavy metals were clearly soluble in the artificial gastric fluid, the careful handling of this residue is recommended in order to prevent the penetration of green liquor dregs across the human gastrointestinal tract.

Keywords: Dregs, non-process elements, pulping, waste.

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3389 Sensitivity Analysis for Determining Priority of Factors Controlling SOC Content in Semiarid Condition of West of Iran

Authors: Y. Parvizi, M. Gorji, M.H. Mahdian, M. Omid

Abstract:

Soil organic carbon (SOC) plays a key role in soil fertility, hydrology, contaminants control and acts as a sink or source of terrestrial carbon content that can affect the concentration of atmospheric CO2. SOC supports the sustainability and quality of ecosystems, especially in semi-arid region. This study was conducted to determine relative importance of 13 different exploratory climatic, soil and geometric factors on the SOC contents in one of the semiarid watershed zones in Iran. Two methods canonical discriminate analysis (CDA) and feed-forward back propagation neural networks were used to predict SOC. Stepwise regression and sensitivity analysis were performed to identify relative importance of exploratory variables. Results from sensitivity analysis showed that 7-2-1 neural networks and 5 inputs in CDA models output have highest predictive ability that explains %70 and %65 of SOC variability. Since neural network models outperformed CDA model, it should be preferred for estimating SOC.

Keywords: Soil organic carbon, modeling, neural networks, CDA.

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3388 Performance Evaluation of Qos Parameters in Cognitive Radio Using Genetic Algorithm

Authors: Maninder Jeet Kaur, Moin Uddin, Harsh K. Verma

Abstract:

The efficient use of available licensed spectrum is becoming more and more critical with increasing demand and usage of the radio spectrum. This paper shows how the use of spectrum as well as dynamic spectrum management can be effectively managed and spectrum allocation schemes in the wireless communication systems be implemented and used, in future. This paper would be an attempt towards better utilization of the spectrum. This research will focus on the decision-making process mainly, with an assumption that the radio environment has already been sensed and the QoS requirements for the application have been specified either by the sensed radio environment or by the secondary user itself. We identify and study the characteristic parameters of Cognitive Radio and use Genetic Algorithm for spectrum allocation. Performance evaluation is done using MATLAB toolboxes.

Keywords: Cognitive Radio, Fitness Functions, Fuzzy Logic, Quality of Service (QoS)

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3387 OSEME: A Smart Learning Environment for Music Education

Authors: Konstantinos Sofianos, Michael Stefanidakis

Abstract:

Nowadays, advances in information and communication technologies offer a range of opportunities for new approaches, methods, and tools in education and training. Teacher-centered learning has changed to student-centered learning. E-learning has now matured and enables the design and construction of intelligent learning systems. A smart learning system fully adapts to a student's needs and provides them with an education based on their preferences, learning styles, and learning backgrounds. It is a wise friend and available at anytime, anywhere, and with any digital device. In this paper, we propose an intelligent learning system, which includes an ontology with all elements of the learning process (learning objects, learning activities) and a massive open online course (MOOC) system. This intelligent learning system can be used in music education.

Keywords: Intelligent learning systems, e-learning, music education, ontology, semantic web.

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3386 Nano Effects of Nitrogen Ion Implantation on TiN Hard Coatings Deposited by PVD and IBAD

Authors: Branko Skoric, Aleksandar Miletic, Pal Terek, Lazar Kovacevic, Milan Kukuruzovic

Abstract:

In this paper, we present the results of a study of TiN thin films which are deposited by a Physical Vapour Deposition (PVD) and Ion Beam Assisted Deposition (IBAD). In the present investigation the subsequent ion implantation was provided with N5+ ions. The ion implantation was applied to enhance the mechanical properties of surface. The thin film deposition process exerts a number of effects such as crystallographic orientation, morphology, topography, densification of the films. A variety of analytic techniques were used for characterization, such as scratch test, calo test, Scanning electron microscopy (SEM), Atomic Force Microscope (AFM), X-ray diffraction (XRD) and Energy Dispersive X-ray analysis (EDAX).

Keywords: Steel, coating, super hard, ion implantation, nanohardness.

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3385 Research on Modern Semiconductor Converters and the Usage of SiC Devices in the Technology Centre of Ostrava

Authors: P. Vaculík, P. Kaňovský

Abstract:

The following article presents Technology Centre of Ostrava (TCO) in the Czech Republic describing the structure and main research areas realized by the project ENET - Energy Units for Utilization of non Traditional Energy Sources. More details are presented from the research program dealing with transformation, accumulation and distribution of electric energy. Technology Centre has its own energy mix consisting of alternative sources of fuel sources that use of process gases from the storage part and also the energy from distribution network. The article will be focus on the properties and application possibilities SiC semiconductor devices for power semiconductor converter for photovoltaic systems.

Keywords: SiC, Si, Technology Centre of Ostrava, Photovoltaic Systems, DC/DC Converter, Simulation.

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3384 Business Rules for Data Warehouse

Authors: Rajeev Kaula

Abstract:

Business rules and data warehouse are concepts and technologies that impact a wide variety of organizational tasks. In general, each area has evolved independently, impacting application development and decision-making. Generating knowledge from data warehouse is a complex process. This paper outlines an approach to ease import of information and knowledge from a data warehouse star schema through an inference class of business rules. The paper utilizes the Oracle database for illustrating the working of the concepts. The star schema structure and the business rules are stored within a relational database. The approach is explained through a prototype in Oracle-s PL/SQL Server Pages.

Keywords: Business Rules, Data warehouse, PL/SQL ServerPages, Relational model, Web Application.

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3383 The Effect of Goat Milk Fractions Supplementation on Serum IgE Response and Leukocytes Count in Dinitrochlorobenzene Sensitized Rat

Authors: Nurliyani, E. Harmayani, MHNE. Soesatyo

Abstract:

In Indonesia, goat milk is often consumed and believed as anti-allergy. The objective of this research was to study the effect of goat milk and their fractions (casein and whey) supplementation on total serum IgE concentrations and leukocytes count in rat sensitized with contact allergen dinitrochlorobenzene (DNCB). Female Wistar rats 6-8 weeks old were divided into four groups: 1) whey, 2) casein, 3) whole milk supplementation and 4) phosphate-buffered saline/PBS (control). The results showed that supplementation of goat milk on rats did not affects on total serum IgE concentrations and number of leukocytes. After sensitized with DNCB, the monocyte percentage in rats was higher (P<0.01) than before. In conclusion, goat milk or their fractions supplementation unable to decrease the total serum IgE concentrations and also had no effect on leukocytes count. However, 1% DNCB could increase the number of monocytes, but could not induce the IgE response.

Keywords: Dinitrochlorobenzene, Goat Milk Fractions, IgE, Leukocytes.

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3382 Restarted GMRES Method Augmented with the Combination of Harmonic Ritz Vectors and Error Approximations

Authors: Qiang Niu, Linzhang Lu

Abstract:

Restarted GMRES methods augmented with approximate eigenvectors are widely used for solving large sparse linear systems. Recently a new scheme of augmenting with error approximations is proposed. The main aim of this paper is to develop a restarted GMRES method augmented with the combination of harmonic Ritz vectors and error approximations. We demonstrate that the resulted combination method can gain the advantages of two approaches: (i) effectively deflate the small eigenvalues in magnitude that may hamper the convergence of the method and (ii) partially recover the global optimality lost due to restarting. The effectiveness and efficiency of the new method are demonstrated through various numerical examples.

Keywords: Arnoldi process, GMRES, Krylov subspace, systems of linear equations.

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3381 Laser Doppler Flowmetry in Diagnostics of Vascular Lesions in Lower Extremities

Authors: Petr V. Vasilev, Eduard V. Volkov, Alexej N. Godok, Alexej A, Grischschuk, Vitalij A. Rybalchenko

Abstract:

Laser Doppler flowmetry is a modern method of noninvasive microcirculation investigation. The aim of our study was to use this method in the examination of patients with secondary lymphedema of the lower extremities and obliterating atherosclerosis of lower extremities. In the analysis of the amplitude-frequency spectrum of secondary lymphedema patients we have identified remarkable changes. To describe the changes we used a special amplitude rate. In both of patients groups this rate was significally (p<0.05) different with the control group. So the marker phenomena of the amplitude-frequency spectrum of the LDF signal were identified. It is suggested that there is a limfodynamics contribution to the formation of the output signal of laser Doppler flowmetry. These data have fundamental meaning and are interesting for practical medicine, as they give an opportunity to further developments for the use of laser Doppler flowmetry in the diagnostics and monitoring the effectiveness of the treatment.

Keywords: laser Doppler flowmetry, secondary lymphedema of the lower extremities, obliterating atherosclerosis, non-invasive diagnostics.

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3380 The Effectiveness of ICT-Assisted PBL on College-Level Nano Knowledge and Learning Skills

Authors: Ya-Ting Carolyn Yang, Ping-Han Cheng, Shi-Hui Gilbert Chang, Terry Yuan-Fang Chen, Chih-Chieh Li

Abstract:

Nanotechnology is widely applied in various areas so professionals in the related fields have to know more than nano knowledge. In the study, we focus on adopting ICT-assisted PBL in college general education to foster professionals who possess multiple abilities. The research adopted a pretest and posttest quasi-experimental design. The control group received traditional instruction, and the experimental group received ICT-assisted PBL instruction. Descriptive statistics will be used to describe the means, standard deviations, and adjusted means for the tests between the two groups. Next, analysis of covariance (ANCOVA) will be used to compare the final results of the two research groups after 6 weeks of instruction. Statistics gathered in the end of the research can be used to make contrasts. Therefore, we will see how different teaching strategies can improve students’ understanding about nanotechnology and learning skills.

Keywords: Nanotechnology, science education, project-based learning, information and communication technology.

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3379 Evaluation of South African Plants with Acaricide Activity against Ticks

Authors: G. Fouché, J. N. Eloff, K. Wellington

Abstract:

Acaricides are commonly used to control ticks but are toxic, harmful to the environment and too expensive to resource-limited farmers. Traditionally, many communities in South Africa rely on a wide range of indigenous practices to keep their livestock healthy. One of these health care practices includes the use of medicinal plants and this offers an alternative to conventional medicine. An investigation was conducted at the CSIR in South Africa, and selected indigenous plants used in communities were scientifically evaluated for the management of ticks in animals. 17 plants were selected from 239 plants used traditionally in South Africa. Two different organic extracts were prepared from the 17 samples, resulting in 34 plant samples. These were tested for efficacy against two tick species, namely Rhipicephalus microplus and Rhipicephalus turanicus. The plant extracts were also screened against Vero cells and most were found to have low cytotoxicity. This study has shown that there is potential for the development of botanicals as natural acaricides against ticks that are non-toxic and environmentally benign.

Keywords: Rhipicephalus microplus, Rhipicephalus turanicus, ticks, plant extracts, South Africa.

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3378 Characterization of Liver Leukocyte Infiltrates and Features of Cytokine Profile under Viral Hepatitis-Induced Immunosuppression

Authors: Olga V. Lebedinskaya, Irina N. Kabanovskaya, Anna S. Lasareva, Nelly K. Akhmatova, Anatoliy P. Godovalov, Andrey V. Horinko, Mikhail V. Kiselevsky

Abstract:

The nature, prevalence, cellular composition of leukocyte infiltrates and immunohistochemical characteristics of their constituent cells in the liver of patients with chronic viral hepatitis B and C were investigated. It was found that the area of distribution and cellular composition of infiltrates depended on the virus type and process activity. The expediency of immunohistochemical study using leukocyte infiltrates from liver biopsies of patients with viral hepatitis aimed at clarifying diagnosis, making prognosis, and choice of optimal treatment with elements of immune correction is emphasized.

Keywords: Viral hepatitis, leukocyte infiltration, immunohistochemical characteristics, immunosupression.

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3377 Chain Extender on Property Relationships of Polyurethane Derived from Soybean Oil

Authors: Flora Elvistia Firdaus

Abstract:

Polyurethane foams (PUF) has been prepared from vegetable; soybean based polyols. They were characterized into flexible and semi rigid polyurethane foam. This work is directed to production of flexible polyurethane foams by a process involving the reaction of mixture of 2,4- and 2,6-Toluene di Isocyanate isomers, with portion of to blends of soy polyols with petroleum polyol in the presence of other ingredients such as blowing agents, silicone surfactants and accelerating agents. Additon of chain extender improves the property then further decreases the properties on further addition of the same. The objective of this work was to study the effect of chain extender and role of phosphoric acid catalyst to the final properties and correlate the morphology image with mechanical properties of these foams.

Keywords: polyurethane, soy polyol, chain extender

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3376 Feasibility Study on Vanillin Production from Jatropha curcas Stem Using Steam Explosion as a Pretreatment

Authors: Pilanee Vaithanomsat, Waraporn Apiwatanapiwat

Abstract:

Jatropha curcas stem was analyzed for chemical compositions: 19.11% pentosan, 42.99% alphacellulose and 24.11% lignin based on dry weight of 100-g raw material. The condition to fractionate cellulose, hemicellulose and lignin in J. curcas stem using steam explosion was optimized. The procedure started from cutting J. curcas stem into small pieces and soaked in water for overnight. After that, they were steam exploded at 214 °C and 21 kg/cm2 for 5 min. The obtained hydrolysate contained 1.55 g/L ferulic acid which after that was used as substrate for vanillin production by Aspergillus niger and Pycnoporus cinnabarinus in one-step process. The maximum 0.65 g/L of vanillin were obtained with the conversion rate of 45.2% based on the initial ferulic acid.

Keywords: Vanillin, production, Jatropha curcas stem, steam explosion.

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3375 Separation Characteristics of Dissolved Gases from Water Concurrently Variable Mixed with Exhalations for the Hollow Fiber Membrane

Authors: Pil Woo Heo

Abstract:

Water contains dissolved oxygen that a fish needs to breathe. It is important to increase the amounts of separation of dissolved oxygen from water for diverse applications using the separation system. In this paper, a separation system of dissolved gases from water concurrently variable mixed with the exhalations using a compressor is proposed. This system takes use of exhalations to increase the amounts of separation of dissolved oxygen from water. A compressor with variable off-time and on-time is used to control the exhalations mixed with inlet water. Exhalations contain some portion of carbon dioxide, oxygen, and nitrogen. Separation of dissolved gases containing dissolved oxygen is enhanced by using exhalations. The amounts of separation and the compositions of carbon dioxide and oxygen are measured. Higher amounts of separation can make the size of the separation device smaller, and then, application areas are diversified.

Keywords: Concurrently, variable mixed, exhalations, separation, hollow fiber.

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3374 Computational Intelligence Techniques and Agents- Technology in E-learning Environments

Authors: Konstantinos C. Giotopoulos, Christos E. Alexakos, Grigorios N. Beligiannis, Spiridon D.Likothanassis

Abstract:

In this contribution a newly developed e-learning environment is presented, which incorporates Intelligent Agents and Computational Intelligence Techniques. The new e-learning environment is constituted by three parts, the E-learning platform Front-End, the Student Questioner Reasoning and the Student Model Agent. These parts are distributed geographically in dispersed computer servers, with main focus on the design and development of these subsystems through the use of new and emerging technologies. These parts are interconnected in an interoperable way, using web services for the integration of the subsystems, in order to enhance the user modelling procedure and achieve the goals of the learning process.

Keywords: Computational Intelligence, E-learning Environments, Intelligent Agents, User Modelling, Bayesian Networks.

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3373 Power Flow Control with UPFC in Power Transmission System

Authors: Samina Elyas Mubeen, R. K. Nema, Gayatri Agnihotri

Abstract:

In this paper the performance of unified power flow controller is investigated in controlling the flow of po wer over the transmission line. Voltage sources model is utilized to study the behaviour of the UPFC in regulating the active, reactive power and voltage profile. This model is incorporated in Newton Raphson algorithm for load flow studies. Simultaneous method is employed in which equations of UPFC and the power balance equations of network are combined in to one set of non-linear algebraic equations. It is solved according to the Newton raphson algorithm. Case studies are carried on standard 5 bus network. Simulation is done in Matlab. The result of network with and without using UPFC are compared in terms of active and reactive power flows in the line and active and reactive power flows at the bus to analyze the performance of UPFC.

Keywords: Newton-Raphson algorithm, Load flow, Unified power flow controller, Voltage source model.

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3372 Differences in Enhancing Enrollment System between Web Application and Mobile Application for Rangsit University, Thailand

Authors: Thossaporn Thossansin, Auttapon Pomsathit

Abstract:

This paper presents a comparison between using a desktop web application and a mobile application for students enrolling in courses at Rangsit University, Thailand. In addition, Rangsit University has enhanced the enrollment process by leveraging its information systems, which allows students to choose to enroll in courses online. In order to use the system, students must provide their identification and personal documents for registration. The reason to have a mobile application is to support students’ ability to access the system at anytime, anywhere and anyplace. The objective of this paper was to: 1. Evaluate the success of developing a user friendly mobile device system and 2. Measure user interest in future mobile development.

Keywords: Mobile Application, Web Application and Enrollment System.

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3371 Integrating Blogging into Peer Assessment on College Students’ English Writing

Authors: Su-Lien Liao

Abstract:

Most of college students in Taiwan do not have sufficient English proficiency to express themselves in written English. Teachers spent a lot of time correcting the errors in students’ English writing, but the results are not satisfactory. This study aims to use blogs as a teaching and learning tool in written English. Before applying peer assessment, students should be trained to be good reviewers.  The teacher starts the course by posting the error analysis of students’ first English composition on blogs as the comment models for students. Then the students will go through the process of drafting, composing, peer response and last revision on blogs. Evaluation questionnaires and interviews will be conducted at the end of the course to see the impact and also students’ perception for the course.

Keywords: Blog, Peer assessment, English writing, Error analysis.

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3370 Localizing Acoustic Touch Impacts using Zip-stuffing in Complex k-space Domain

Authors: R. Bremananth, Andy W. H. Khong, A. Chitra

Abstract:

Visualizing sound and noise often help us to determine an appropriate control over the source localization. Near-field acoustic holography (NAH) is a powerful tool for the ill-posed problem. However, in practice, due to the small finite aperture size, the discrete Fourier transform, FFT based NAH couldn-t predict the activeregion- of-interest (AROI) over the edges of the plane. Theoretically few approaches were proposed for solving finite aperture problem. However most of these methods are not quite compatible for the practical implementation, especially near the edge of the source. In this paper, a zip-stuffing extrapolation approach has suggested with 2D Kaiser window. It is operated on wavenumber complex space to localize the predicted sources. We numerically form a practice environment with touch impact databases to test the localization of sound source. It is observed that zip-stuffing aperture extrapolation and 2D window with evanescent components provide more accuracy especially in the small aperture and its derivatives.

Keywords: Acoustic source localization, Near-field acoustic holography (NAH), FFT, Extrapolation, k-space wavenumber errors.

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3369 Cloning and Over Expression of an Aspergillus niger XP Phytase Gene (phyA) in Pichia pastoris

Authors: Ngo Thanh Xuan, Mai Thi Hang, Vu Nguyen Thanh

Abstract:

A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 - kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide α- factor, under the control of AOX1 promoter. The recombined plasmid was transformed into the host strain P. pastoris KM71H and X33 by electroporation. Both host strains could efficiently express and secret phytase. The multicopy strains were screened for over expression of phytase. All the selected multicopy strains of P. pastoris X33 were examined for phytase activity, the maximum phytase yield of 1329 IU/ml was obtained after 4 days of incubation in medium BMM. The recombinant protein with MW of 97.4 KW showed to be the only one protein secreted in the culture broth. Multicopy transformant P. pastoris X33 supposed to be potential candidate for producing the commercial preparation of phytase.

Keywords: Aspergillus niger XP, cloning, over expression, Pichia pastoris, phyA , phytase.

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3368 Mutational Analysis of CTLA4 Gene in Pakistani SLE Patients

Authors: N. Hussain, G. Jaffery, A.N. Sabri, S. Hasnain

Abstract:

The main aim is to perform mutational analysis of CTLA4 gene Exon 1 in SLE patients. A total of 61 SLE patients fulfilling “American College of Rheumatology (ACR) criteria" and 61 controls were enrolled in this study. The region of CTLA4 gene exon 1 was amplified by using Step-down PCR technique. Extracted DNA of band 354 bp was sequenced to analyze mutations in the exon-1 of CTLA-4 gene. Further, protein sequences were identified from nucleotide sequences of CTLA4 Exon 1 by using Expasy software and through Blast P software it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. No variations were found after patients sequences were compared with that of the control sequence. Furthermore it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. Thus CTLA4 gene may not be responsible for an autoimmune disease SLE.

Keywords: American College of Rheumatology criteria, autoimmune disease, Cytotoxic T Lymphocyte Antigen-4, Polymerase Chain Reaction, Systemic Lupus Erythematosus

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3367 A Matrix Evaluation Model for Sustainability Assessment of Manufacturing Technologies

Authors: Q. Z. Yang, B. H. Chua, B. Song

Abstract:

Technology assessment is a vital part of decision process in manufacturing, particularly for decisions on selection of new sustainable manufacturing processes. To assess these processes, a matrix approach is introduced and sustainability assessment models are developed. Case studies show that the matrix-based approach provides a flexible and practical way for sustainability evaluation of new manufacturing technologies such as those used in surface coating. The technology assessment of coating processes reveals that compared with powder coating, the sol-gel coating can deliver better technical, economical and environmental sustainability with respect to the selected sustainability evaluation criteria for a decorative coating application of car wheels.

Keywords: Evaluation matrix, sustainable manufacturing, surface coating, technology assessment

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3366 A Fuzzy Logic Based Navigation of a Mobile Robot

Authors: Anis Fatmi, Amur Al Yahmadi, Lazhar Khriji, Nouri Masmoudi

Abstract:

One of the long standing challenging aspect in mobile robotics is the ability to navigate autonomously, avoiding modeled and unmodeled obstacles especially in crowded and unpredictably changing environment. A successful way of structuring the navigation task in order to deal with the problem is within behavior based navigation approaches. In this study, Issues of individual behavior design and action coordination of the behaviors will be addressed using fuzzy logic. A layered approach is employed in this work in which a supervision layer based on the context makes a decision as to which behavior(s) to process (activate) rather than processing all behavior(s) and then blending the appropriate ones, as a result time and computational resources are saved.

Keywords: Behavior based navigation, context based coordination, fuzzy logic, mobile robots.

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