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Mutational Analysis of CTLA4 Gene in Pakistani SLE Patients

Authors: N. Hussain, G. Jaffery, A.N. Sabri, S. Hasnain

Abstract:

The main aim is to perform mutational analysis of CTLA4 gene Exon 1 in SLE patients. A total of 61 SLE patients fulfilling “American College of Rheumatology (ACR) criteria" and 61 controls were enrolled in this study. The region of CTLA4 gene exon 1 was amplified by using Step-down PCR technique. Extracted DNA of band 354 bp was sequenced to analyze mutations in the exon-1 of CTLA-4 gene. Further, protein sequences were identified from nucleotide sequences of CTLA4 Exon 1 by using Expasy software and through Blast P software it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. No variations were found after patients sequences were compared with that of the control sequence. Furthermore it was found that CTLA4 protein sequences of Pakistani SLE patients were similar to that of Chinese SLE population. Thus CTLA4 gene may not be responsible for an autoimmune disease SLE.

Keywords: American College of Rheumatology criteria, autoimmune disease, Cytotoxic T Lymphocyte Antigen-4, Polymerase Chain Reaction, Systemic Lupus Erythematosus

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1074485

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References:


[1] C. Kek-Hang, P. Suat-Moi, C. Ching-Hoong, T. Si-Yen, L. Lay-Hoong, L," Study of CTLA-4 gene polymorphisms in SLE samples from Malaysia". Annals of Human Biology, 2010, 37:274-280.
[2] M.L. Budarf, P. Goyette, G. Boucher, J. Lian, R.R. Graham, J.O. Claudio,T. Hudson, D. Gladman, A.F. Clarke, Pope, A.E., Peschken, et al "A targeted association study in SLE identifies multiple susceptibility alleles". Genes and immunity, 2010, 10:1038.
[3] H. Bashyam, "CTLA-4: From conflict to clinic". J Exp Med, 2007, 204:1243.
[4] D. Holmberg , C.M. Cilio, M. Lundholm, V. Motta, "CTLA-4 (CD152) and its involvement in autoimmune disease". Autoimmunity, 2005; 38: 225 - 233