Search results for: Red yeast rice.
144 Response of Yield and Morphological Characteristic of Rice Cultivars to Heat Stress at Different Growth Stages
Authors: M. T. K. Aghamolki, M. K. Yusop, F. C. Oad, H. Zakikhani, Hawa. Ze Jaafar, S. Kharidah S.M., M. M. Hanafi
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The high temperatures during sensitive growth phases are changing rice morphology as well as influencing yield. In the glass house study, the treatments were growing conditions [normal growing (32oC+2) and heat stress (38oC+2) day time and 22oC+2 night time], growth stages (booting, flowering and ripening) and four cultivars (Hovaze, Hashemi, Fajr, as exotic and MR219 as indigenous). The heat chamber was prepared covered with plastic, and automatic heater was adjusted for two weeks in every growth stages. Rice morphological and yield under the influence of heat stress during various growth stages showed taller plants in Hashemi due to its tall character. The total tillers per hill were significantly higher in Fajr. In all growing conditions, Hashemi recorded higher panicle exertion. The flag leaf width in all situations was found higher in Hovaze. The total tillers per hill were more in Fajr, although heat stress was imposed during booting and flowering stages. The indigenous MR219 in all situations of growing conditions, growth stages recorded higher grain yield. However, its grain yield decreased when heat stress was imposed during booting and flowering. However, plants had no effect on heat stress during ripening stage.
Keywords: Rice, growth, heat, stress, morphology, yield.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3358143 Analysis of a Lignocellulose Degrading Microbial Consortium to Enhance the Anaerobic Digestion of Rice Straws
Authors: Supanun Kangrang, Kraipat Cheenkachorn, Kittiphong Rattanaporn, Malinee Sriariyanun
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Rice straw is lignocellulosic biomass which can be utilized as substrate for the biogas production. However, due to the property and composition of rice straw, it is difficult to be degraded by hydrolysis enzymes. One of the pretreatment methods that modify such properties of lignocellulosic biomass is the application of lignocellulose-degrading microbial consortia. The aim of this study is to investigate the effect of microbial consortia to enhance biogas production. To select the high efficient consortium, cellulase enzymes were extracted and their activities were analyzed. The results suggested that microbial consortium culture obtained from cattle manure is the best candidate compared to decomposed wood and horse manure. A microbial consortium isolated from cattle manure was then mixed with anaerobic sludge and used as inoculum for biogas production. The optimal conditions for biogas production were investigated using response surface methodology (RSM). The tested parameters were the ratio of amount of microbial consortium isolated and amount of anaerobic sludge (MI:AS), substrate to inoculum ratio (S:I) and temperature. Here, the value of the regression coefficient R2 = 0.7661 could be explained by the model which is high to advocate the significance of the model. The highest cumulative biogas yield was 104.6 ml/g-rice straw at optimum ratio of MI:AS, ratio of S:I, and temperature of 2.5:1, 15:1 and 44°C respectively.
Keywords: Lignocellulolytic biomass, microbial consortium, cellulase, biogas, Response Surface Methodology.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3324142 MIBiClus: Mutual Information based Biclustering Algorithm
Authors: Neelima Gupta, Seema Aggarwal
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Most of the biclustering/projected clustering algorithms are based either on the Euclidean distance or correlation coefficient which capture only linear relationships. However, in many applications, like gene expression data and word-document data, non linear relationships may exist between the objects. Mutual Information between two variables provides a more general criterion to investigate dependencies amongst variables. In this paper, we improve upon our previous algorithm that uses mutual information for biclustering in terms of computation time and also the type of clusters identified. The algorithm is able to find biclusters with mixed relationships and is faster than the previous one. To the best of our knowledge, none of the other existing algorithms for biclustering have used mutual information as a similarity measure. We present the experimental results on synthetic data as well as on the yeast expression data. Biclusters on the yeast data were found to be biologically and statistically significant using GO Tool Box and FuncAssociate.
Keywords: Biclustering, mutual information.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1631141 Evaluation of the Triticale Flour Blend Dough in the Mixing and Fermentation Processes
Authors: Martins Sabovics, Karina Ruse, Evita Straumite, Ruta Galoburda
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The research was accomplished on triticale flour blend, which was made from whole grain triticale, rye, hull-less barley flour and rice, maize flour. The aim of this research was to evaluate physico-chemical and sensory properties of triticale flour blend dough in the mixing and fermentation processes. For dough making was used triticale flour blend, yeast, sugar, salt, and water. In the mixing process ware evaluated moisture, acidity, pH, and dough sensory properties (softness, viscosity, and stickiness), but in the fermentation process ware evaluated volume, moisture, acidity, and pH. During present research was established that increasing fermentation temperature and time, increase dough temperature, volume, moisture, and acidity. The mixing time and fermentation time and temperature have significant effect (p<0.05) on triticale flour blend dough physico-chemical and sensory properties.
Keywords: Dough quality, dough fermentation, dough mixing, triticale flour blend.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2467140 The Composition of Rice Bran Hydrolysate and Its Possibility to Use in the Ethanol Production by Zymomonas mobilis Biofilm
Authors: Tatsaporn Todhanakasem, Kamonchanok Areerat, Pornthap Thanonkeo, Roungdao KlinjapoandGlenn M. Young
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Rice bran has been abandoned as agricultural waste for million tonnes per year in Thailand, therefore they have been proposed to be utilized as a rich carbon source in the production of bioethanol. Many toxic compounds are possibly released during the pretreatment of rice bran prior the fermentation process. This study aims to analyze on the availability of toxic compounds and the amount of glucose obtained from 2 different pretreatments using sulfuric acid and mixed cellulase enzymes (without and with delignification/ activated charcoal). The concentration of furfural, 5- hydroxymethyl furfural (5-HMF), levulinic acid, vanillin, syringaldehyde and4-hydroxybenzaldehyde (4-HB) and the percent acetic acid were found to be 0.0517 ± 0.049 mg/L, 0.032 ± 0.06 mg/L, 21074 ± 1685.62 mg/L, 126.265 ± 6.005 mg/L, 2.89 ± 0.30 mg/L, 0.37 ± 0.031mg/L and 0.72% under the pretreatment process without delignification/ activated charcoal treatment and 384.47 ± 99.02 g/L, 0.068 mg/L, 142107.62 ± 8664.6 mg/L, 0.19 mg/L, 5.43 ± 3.29 mg/L, 4.80 ± 0.76 mg/L and 0.254% under the pretreatment process with delignification/ activated charcoal treatment respectively. The presence of high concentration of acetic acid was found to impede the growth of Zymomonas mobilis strain TISTR 551 despite the present of high concentration of levulinic acid. Z. mobilis strain TISTR 551 was found to produce 8.96 ± 4.06 g/L of ethanol under 4 days fementation period in biofilm stage in which represented 40% theoretical yield.
Keywords: Rice bran, Zymomonas mobilis, biofilm, ethanol.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2721139 Statistical Screening of Medium Components on Ethanol Production from Cashew Apple Juice using Saccharomyces diasticus
Authors: Karuppaiya Maruthai, Viruthagiri Thangavelu, Manikandan Kanagasabai
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In the present study, effect of critical medium components (a total of fifteen components) on ethanol production from waste cashew apple juice (CAJ) using yeast Saccharomyces diasticus was studied. A statistical response surface methodology (RSM) based Plackett-Burman Design (PBD) was used for the design of experiments. The design contains a total of 32 experimental trails. The effect of medium components on ethanol was studied at two different levels such as low concentration level (-) and high concentration levels (+). The dependent variables selected in this study were ethanol concentration (g/L) and cellmass concentration (g/L). Data obtained from RSM on ethanol production were subjected to analysis of variance (ANOVA). In general, initial substrate concentration significantly influenced the microbial growth and product formation. Of the medium components evaluated, CAJ concentration, yeast extract, (NH4)2SO4, and malt extract showed significant effect on ethanol fermentation. A second-order polynomial model was used to predict the experimental data and the model fitted the data with a high correlation coefficient (R2 > 0.98). Maximum ethanol (15.3 g/L) and biomass (6.4 g/L) concentrations were obtained at the optimum medium composition and at optimum condition (temperature-30°C; initial pH-6.8) after 72 h fermentation using S.diasticus.
Keywords: cashew apple juice, ethanol, fermentation, yeast, response surface methodology
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2712138 Analyzing Irbid’s Food Waste as Feedstock for Anaerobic Digestion
Authors: Assal E. Haddad
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Food waste samples from Irbid were collected from 5 different sources for 12 weeks to characterize their composition in terms of four food categories; rice, meat, fruits and vegetables, and bread. Average food type compositions were 39% rice, 6% meat, 34% fruits and vegetables, and 23% bread. Methane yield was also measured for all food types and was found to be 362, 499, 352, and 375 mL/g VS for rice, meat, fruits and vegetables, and bread, respectively. A representative food waste sample was created to test the actual methane yield and compare it to calculated one. Actual methane yield (414 mL/g VS) was greater than the calculated value (377 mL/g VS) based on food type proportions and their specific methane yield. This study emphasizes the effect of the types of food and their proportions in food waste on the final biogas production. Findings in this study provide representative methane emission factors for Irbid’s food waste, which represent as high as 68% of total Municipal Solid Waste (MSW) in Irbid, and also indicate the energy and economic value within the solid waste stream in Irbid.
Keywords: Food waste, solid waste management, anaerobic digestion, methane yield.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 823137 Use of GIS for the Performance Evaluation of Canal Irrigation System in Rice Wheat Cropping Zone
Authors: Umm-e- Kalsoom, M. Arshad, Sadia Iqbal, M. Usman, M. Adnan
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The research study evaluated the performance of irrigation system by using special scientific tools like Remote Sensing and GIS technology, so that proper measurements could be taken for the sustainable agriculture and water management. Different performance evaluation parameters had been calculated for the purposed data was gathered from field investigation and different government and private organizations. According to the calculations, organic matter ranges from 0.19% (low value) to 0.76% (high value). In flat irrigation system for wheat yield ranges from 3347.16 to 5260.39 kg/ha, while the total water applied to wheat crop ranges from 252.94 to 279.19 mm and WUE ranges from 13.07 to 18.37 kg/ha/mm. For rice yield ranges from 3347.47 to 5433.07 kg/ha with total water supplied to rice crop ranges from 764.71 to 978.15 mm and WUE ranges from 3.49 to 5.71 kg/ha/mm. Similarly, in raised bed system wheat yield ranges from 4569.13 to 6008.60 kg/ha, total water supplied ranges from 158.87 to 185.09 mm and WUE ranges from 27.20 to 33.54 kg/ha/mm while in rice crop, yield ranges from 5285.04 to 6716.69 kg/ha, total water supplied ranges from 600.72 to 755.06 mm and WUE ranges from 6.41 to 10.05 kg/ha/mm. Almost 51.3% water saving is observed in bed irrigation system as compared to flat system. Less water supplied to beds is more affective as its WUE value is higher than flat system where more water is supplied in both the seasons. Similarly, RWS values show that maximum water deficit while minimum area is getting adequate water supply. Greater yield is recorded in bed system as plant per square meter is more in bed system in comparison of flat system Thus, the integration of GIS tools to regularly compute performance indices could provide irrigation managers with the means for managing efficiently the irrigation system.Keywords: Field survey, Relative Water Supply (RWS), Remote sensing maps, Water Use Efficiency (WUE).
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2418136 Long Term Effect of Rice Husk Ash on Strength of Mortar
Authors: A. Md. Harunur Rashid B. Md. Keramat Ali Molla C. Tarif Uddin Ahmed
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This paper represents the results of long term strength of mortar incorporating Rice Husk Ash (RHA). For these work mortar samples were made according to ASTM standard C 109/C. OPC cement was partially replaced by RHA at 0, 10, 15, 20, 25 and 30 percent replacement level. After casting all samples were kept in controlled environment and curing was done up to 90 days. Test of mortar was performed on 3, 7, 28, 90, 365 and 700 days. It is noticed that OPC mortar shows better strength at early age than mortar having RHA but at 90 days and onward the picture is different. At 700 days it is observed that mortar containing 20% RHA shows better result than any other samples.
Keywords: OPC, RHA, replacement level, long term, strength.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1967135 Seasonal Prevalence of Aedes aegypti and Ae.albopictus in Three Topographical Areas of Southern Thailand
Authors: W. Preechaporn, M. Jaroensutasinee, K. Jaroensutasinee
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This study investigated the seasonal prevalence of Aedes aegypti and Ae. albopictus larvae in three topographical areas (i.e. mangrove, rice paddy and mountainous areas). Samples were collected from 300 households in both wet and dry seasons in nine districts in Nakhon Si Thammarat province. Ae. aegypti and Ae. albopictus were found in 21 out of 29 types of water containers in mangrove, rice paddy and mountainous areas. Ae. aegypti and Ae. albopictus laid eggs in different container types depending on season and topographical areas. Ae. aegypti larvae were found most in metal box in mangrove and mountainous areas in wet season. Ae. albopictus larvae were also found most in metal box in mangrove and mountainous areas in both wet and dry seasons. All Ae. albopictus larval indices were higher than Ae. aegypti larval indices in all three topographical areas and both seasons. HI and BI did not differ in three topographical areas but differed between Aedes sp. HI for both Ae. aegypti and Ae. albopictus in all three topographical areas in both seasons were greater than 10 %, except Aedes aegypti in rice paddy area in wet season. This indicated high risks of DHF transmission in these areas.Keywords: Aedes aegypti, Aedes albopictus, Season, Topography.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1811134 Fluidised Bed Gasification of Multiple Agricultural Biomass Derived Briquettes
Authors: Rukayya Ibrahim Muazu, Aiduan Li Borrion, Julia A. Stegemann
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Biomass briquette gasification is regarded as a promising route for efficient briquette use in energy generation, fuels and other useful chemicals. However, previous research has been focused on briquette gasification in fixed bed gasifiers such as updraft and downdraft gasifiers. Fluidised bed gasifier has the potential to be effectively sized to medium or large scale. This study investigated the use of fuel briquettes produced from blends of rice husks and corn cobs biomass, in a bubbling fluidised bed gasifier. The study adopted a combination of numerical equations and Aspen Plus simulation software, to predict the product gas (syngas) composition base on briquette density and biomass composition (blend ratio of rice husks to corn cobs). The Aspen Plus model was based on an experimentally validated model from the literature. The results based on a briquette size 32 mm diameter and relaxed density range of 500 to 650kg/m3, indicated that fluidisation air required in the gasifier increased with increase in briquette density, and the fluidisation air showed to be the controlling factor compared with the actual air required for gasification of the biomass briquettes. The mass flowrate of CO2 in the predicted syngas composition increased with an increase in air flow, in the gasifier, while CO decreased and H2 was almost constant. The ratio of H2 to CO for various blends of rice husks and corn cobs did not significantly change at the designed process air, but a significant difference of 1.0 was observed between 10/90 and 90/10 % blend of rice husks and corn cobs.Keywords: Briquettes, fluidised bed, gasification, Aspen Plus, syngas.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2551133 The Evaluation and the Comparison of the Effect of Without Engine Power and Power Mechanical Systems on Rice Weed
Authors: F. E. Cherati, T. Naij , A. Amraei, Y. Hosseinpor
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In order to study the influence of different methods of controlling weeds such as mechanical weeding and mechanical weeder efficiency analysis in mechanical cultivation conditions, in farming year of 2011 an experiment was done in a farm in coupling and development of technology center in Haraz,Iran. The treatments consisted of (I) control treatment: where no weeding was done, (II) use of mechanical weeding without engine and (III) power mechanical weeding. Results showed that experimental treatments had significantly different effects (p=0.05) on yield traits and number of filled grains per panicle, while treatments had the significant effects on grain weight and dry weight of weeds in the first, second and third weeding methods at 1% of confidence level. Treatment (II) had its most significant effect on number of filled grains per panicle and yield performance standpoint, which was 3705.97 kg ha-1 in its highest peak. Treatment (III) was ranked as second influential with 3559.8 kg ha-1. In addition, under (I) treatments, 2364.73 kg ha-1 of yield produced. The minimum dry weights of weeds in all weeding methods were related to the treatment (II), (III) and (I), respectively. The correlation coefficient analysis showed that total yield had a significant positive correlation with the panicle grain yield per plant (r= 0.55*) and the number of grains per panicle-1 (r= 0.57*) and the number of filled grains (r= 0.63*). Total rice yield also had negative correlation of r= -0. 64* with weed dry weight at second weed sampling time (17 DAT). The weed dry weight at third and fourth sampling times (24 and 40 DAT) had negative correlations of -0.65** and r=-0.61* with rice yield, respectively.
Keywords: Dry weight, without engine mechanical weeder, power mechanical weeder, yield rice.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1446132 The Influence of RHA on the Mechanical Properties of Mortar Heated Up To High Temperature
Authors: Md. Harunur Rashid, S. M. Kamal Uddin, Sobura khatun
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The performance of mortar subjected to high temperature and cooled in normal ambient temperature was examined in the laboratory to comply with the situation of burning & cooling of a structure. Four series of cubical (5 X 5 X 5 cm) mortar specimens were made from OPC, and partial replacement (10, 15, 20, 25 & 30%) of OPC by Rice Husk Ash (RHA) produced in the uncontrolled environment. These specimens were heated in electric furnace to 200, 300, 400, 500 and 7000C. The specimens were kept in normal room temperature for cooling. They were then tested for mechanical properties and the results shows that particular 20% RHA mixed mortar shows better fire performance.Keywords: Fire performance, Rice Husk
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1564131 Effect of Rice Husk Ash on Strength and Durability of High Strength High Performance Concrete
Authors: H. B. Mahmud, Syamsul Bahri, Y. W. Yee, Y. T. Yeap
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This paper reports the strength and durability properties of high strength high performance concrete incorporating rice husk ash (RHA) having high silica, low carbon content and appropriate fineness. In this study concrete containing 10%, 15% and 20% RHA as cement replacement and water to binder ratio of 0.25 were investigated. The results show that increasing amount of RHA increases the dosage of superplasticizer to maintain similar workability. Partial replacement of cement with RHA did not increase the early age compressive strength of concrete. However, concrete containing RHA showed higher compressive strength at later ages. The results showed that compressive strength of concrete in the 90-115 MPa range can be obtained at 28 curing days and the durability properties of RHA concrete performed better than that of control concrete. The water absorption of concrete incorporating 15% RHA exhibited the lowest value. The porosity of concrete is consistent with water absorption whereby higher replacement of RHA decreased the porosity of concrete. There is a positive correlation between reducing porosity and increasing compressive strength of high strength high performance concrete. The results also indicate that up to 20% of RHA incorporation could be advantageously blended with cement without adversely affecting the strength and durability properties of concrete.
Keywords: Compressive strength, durability, high performance concrete, rice husk ash.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2734130 Upgraded Rough Clustering and Outlier Detection Method on Yeast Dataset by Entropy Rough K-Means Method
Authors: P. Ashok, G. M. Kadhar Nawaz
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Rough set theory is used to handle uncertainty and incomplete information by applying two accurate sets, Lower approximation and Upper approximation. In this paper, the rough clustering algorithms are improved by adopting the Similarity, Dissimilarity–Similarity and Entropy based initial centroids selection method on three different clustering algorithms namely Entropy based Rough K-Means (ERKM), Similarity based Rough K-Means (SRKM) and Dissimilarity-Similarity based Rough K-Means (DSRKM) were developed and executed by yeast dataset. The rough clustering algorithms are validated by cluster validity indexes namely Rand and Adjusted Rand indexes. An experimental result shows that the ERKM clustering algorithm perform effectively and delivers better results than other clustering methods. Outlier detection is an important task in data mining and very much different from the rest of the objects in the clusters. Entropy based Rough Outlier Factor (EROF) method is seemly to detect outlier effectively for yeast dataset. In rough K-Means method, by tuning the epsilon (ᶓ) value from 0.8 to 1.08 can detect outliers on boundary region and the RKM algorithm delivers better results, when choosing the value of epsilon (ᶓ) in the specified range. An experimental result shows that the EROF method on clustering algorithm performed very well and suitable for detecting outlier effectively for all datasets. Further, experimental readings show that the ERKM clustering method outperformed the other methods.
Keywords: Clustering, Entropy, Outlier, Rough K-Means, validity index.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1412129 rRNA Maturation Genes (KRR1 and PWP2) in Saccharomyces cerevisiae Inhibited by Silver Nanoparticles
Authors: Anjali Haloi, Debabrata Das
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Silver nanoparticles inhibit a wide variety of microorganisms. The mechanism of inhibition is not entirely known although it is recognized to be concentration dependent and associated with the disruption of membrane permeability. Data on differential gene expression as a response to nanoparticles could provide insights into the mechanism of this inhibitory effect. Silver nanoparticles were synthesized in yeast growth media using a modification of the Creighton method and characterized with UV-Vis spectrophotometry, transmission electron microscopy (TEM), and X-ray diffraction (XRD). In yeasts grown in the presence of silver nanoparticles, we observed that at concentrations below the minimum inhibitory concentration (MIC) of 48.51 µg/ml, the total RNA content was steady while the cellular protein content declined rapidly. The analysis of the expression levels of KRR1 and PWP2, two important genes involved in rRNA maturation in yeasts, showed up to 258 and 42-fold decreases, respectively, compared to that of control samples. Whether silver nanoparticles have an adverse effect on ribosome assembly and function could be an area of further investigation.
Keywords: Ag NP, yeast, qRT-PCR, KRR1, PWP2.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 387128 Biodegradation of Lignocellulosic Residues of Water Hyacinth (Eichhornia crassipes) and Response Surface Methodological Approach to Optimize Bioethanol Production Using Fermenting Yeast Pachysolen tannophilus NRRL Y-2460
Authors: A. Manivannan, R. T. Narendhirakannan
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The objective of this research was to investigate biodegradation of water hyacinth (Eichhornia crassipes) to produce bioethanol using dilute-acid pretreatment (1% sulfuric acid) results in high hemicellulose decomposition and using yeast (Pachysolen tannophilus) as bioethanol producing strain. A maximum ethanol yield of 1.14g/L with coefficient, 0.24g g-1; productivity, 0.015g l-1h-1 was comparable to predicted value 32.05g/L obtained by Central Composite Design (CCD). Maximum ethanol yield coefficient was comparable to those obtained through enzymatic saccharification and fermentation of acid hydrolysate using fully equipped fermentor. Although maximum ethanol concentration was low in lab scale, the improvement of lignocellulosic ethanol yield is necessary for large scale production.
Keywords: Acid hydrolysis, Biodegradation, Hemicellulose, Pachysolen tannophilus, Water hyacinth.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1891127 Prophylactic Effects of Dairy Kluyveromyces marxianus YAS through Overexpression of BAX, CASP 3, CASP 8 and CASP 9 on Human Colon Cancer Cell Lines
Authors: Amir Saber Gharamaleki, Beitollah Alipour, Zeinab Faghfoori, Ahmad YariKhosroushahi
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Colorectal cancer (CRC) is one of the most prevalent cancers and intestinal microbial community plays an important role in colorectal tumorigenesis. Probiotics have recently been assessed as effective anti-proliferative agents and thus this study was performed to examine whether CRC undergo apoptosis by treating with isolated Iranian native dairy yeast, Kluyveromyces marxianus YAS, secretion metabolites. The cytotoxicity assessments on cells (HT-29, Caco-2) were accomplished through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as well as qualitative DAPI (4',6-diamidino-2-phenylindole staining) and quantitative (flow cytometry assessments) evaluations of apoptosis. To evaluate the main mechanism of apoptosis, Real time PCR method was applied. Kluyveromyces marxianus YAS secretions (IC50) showed significant cytotoxicity against HT-29 and Caco-2 cancer cell lines (66.57 % and 66.34 % apoptosis) similar to 5-Fluorouracil (5-FU) while apoptosis only was developed in 27.57 % of KDR normal cells. The prophylactic effects of Kluyveromyces marxianus (PTCC 5195), as a reference yeast, was not similar to Kluyveromyces marxianus YAS indicating strain dependency of bioactivities on CRC disease prevention. Based on real time PCR results, the main cytotoxicity is related to apoptosis phenomenon and the core related mechanism is depended on the overexpression of BAX, CASP 9, CASP 8 and CASP 3 inducing apoptosis genes. However, several investigations should be conducted to precisely determine the effective compounds to be used as anticancer therapeutics in the future.Keywords: Anticancer, anti-proliferative, apoptosis, cytotoxicity, yeast.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1661126 Enzymatic Esterification of Carboxylic Acids and Higher Alcohols in Organic Medium
Authors: D.T. Mirzarakhmetova
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The studying of enzymatic esterification of carboxylic acids and higher alcohols was performed by esterase Saccharomyces cerevisiae in water-organic medium. Investigation of the enzyme specificity to acetic substrates showed the best result with acetic acid in esterification reactions with ethanol whereas within other carboxylic acids the esterification decreased with acids: hexanoic > pentanoic > butyric > decanoic. In relation to higher alcohols C3-C5, esterification increased with alcohols propanol < butanol < amylol. Also it was determined that esterase was more specific to alcohols with branched chain such as isobutyl alcohol and isoamyl alcohol. Data obtained may have important practical implications, for example, for application of yeast esterase in producing various volatile esters as well as in enzymatic transformation of volatile acids and toxic fusel alcohols into volatile esters by providing the production of the high quality alcoholic beverages with redused content of higher alcohols as well as with improved degustational and hygienic properties.Keywords: enzymes in non-conventional media, esterification, higher alcohols, volatile esters, yeast esterase
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3500125 Use of Agricultural Waste for the Removal of Nickel Ions from Aqueous Solutions: Equilibrium and Kinetics Studies
Authors: Manjeet Bansal, Diwan Singh, V.K.Garg, Pawan Rose
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The potential of economically cheaper cellulose containing natural materials like rice husk was assessed for nickel adsorption from aqueous solutions. The effects of pH, contact time, sorbent dose, initial metal ion concentration and temperature on the uptake of nickel were studied in batch process. The removal of nickel was dependent on the physico-chemical characteristics of the adsorbent, adsorbate concentration and other studied process parameters. The sorption data has been correlated with Langmuir, Freundlich and Dubinin-Radush kevich (D-R) adsorption models. It was found that Freundlich and Langmuir isotherms fitted well to the data. Maximum nickel removal was observed at pH 6.0. The efficiency of rice husk for nickel removal was 51.8% for dilute solutions at 20 g L-1 adsorbent dose. FTIR, SEM and EDAX were recorded before and after adsorption to explore the number and position of the functional groups available for nickel binding on to the studied adsorbent and changes in surface morphology and elemental constitution of the adsorbent. Pseudo-second order model explains the nickel kinetics more effectively. Reusability of the adsorbent was examined by desorption in which HCl eluted 78.93% nickel. The results revealed that nickel is considerably adsorbed on rice husk and it could be and economic method for the removal of nickel from aqueous solutions.Keywords: Adsorption, nickel, SEM, EDAX.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2683124 Experimental and Theoretical Investigation of Rough Rice Drying in Infrared-assisted Hot Air Dryer Using Artificial Neural Network
Authors: D. Zare, H. Naderi, A. A. Jafari
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Drying characteristics of rough rice (variety of lenjan) with an initial moisture content of 25% dry basis (db) was studied in a hot air dryer assisted by infrared heating. Three arrival air temperatures (30, 40 and 500C) and four infrared radiation intensities (0, 0.2 , 0.4 and 0.6 W/cm2) and three arrival air speeds (0.1, 0.15 and 0.2 m.s-1) were studied. Bending strength of brown rice kernel, percentage of cracked kernels and time of drying were measured and evaluated. The results showed that increasing the drying arrival air temperature and radiation intensity of infrared resulted decrease in drying time. High bending strength and low percentage of cracked kernel was obtained when paddy was dried by hot air assisted infrared dryer. Between this factors and their interactive effect were a significant difference (p<0.01). An intensity level of 0.2 W/cm2 was found to be optimum for radiation drying. Furthermore, in the present study, the application of Artificial Neural Network (ANN) for predicting the moisture content during drying (output parameter for ANN modeling) was investigated. Infrared Radiation intensity, drying air temperature, arrival air speed and drying time were considered as input parameters for the model. An ANN model with two hidden layers with 8 and 14 neurons were selected for studying the influence of transfer functions and training algorithms. The results revealed that a network with the Tansig (hyperbolic tangent sigmoid) transfer function and trainlm (Levenberg-Marquardt) back propagation algorithm made the most accurate predictions for the paddy drying system. Mean square error (MSE) was calculated and found that the random errors were within and acceptable range of ±5% with coefficient of determination (R2) of 99%.
Keywords: Rough rice, Infrared-hot air, Artificial Neural Network
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1826123 Thermogravimetry Study on Pyrolysis of Various Lignocellulosic Biomass for Potential Hydrogen Production
Authors: S.S. Abdullah, S. Yusup, M.M. Ahmad, A. Ramli, L. Ismail
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This paper aims to study decomposition behavior in pyrolytic environment of four lignocellulosic biomass (oil palm shell, oil palm frond, rice husk and paddy straw), and two commercial components of biomass (pure cellulose and lignin), performed in a thermogravimetry analyzer (TGA). The unit which consists of a microbalance and a furnace flowed with 100 cc (STP) min-1 Nitrogen, N2 as inert. Heating rate was set at 20⁰C min-1 and temperature started from 50 to 900⁰C. Hydrogen gas production during the pyrolysis was observed using Agilent Gas Chromatography Analyzer 7890A. Oil palm shell, oil palm frond, paddy straw and rice husk were found to be reactive enough in a pyrolytic environment of up to 900°C since pyrolysis of these biomass starts at temperature as low as 200°C and maximum value of weight loss is achieved at about 500°C. Since there was not much different in the cellulose, hemicelluloses and lignin fractions between oil palm shell, oil palm frond, paddy straw and rice husk, the T-50 and R-50 values obtained are almost similar. H2 productions started rapidly at this temperature as well due to the decompositions of biomass inside the TGA. Biomass with more lignin content such as oil palm shell was found to have longer duration of H2 production compared to materials of high cellulose and hemicelluloses contents.Keywords: biomass, decomposition, hydrogen, lignocellulosic, thermogravimetry
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2268122 Evaluation of Antioxidant Activities of Rice Paddy Herb (Limnophila aromatica (Lam.) Merr.)
Authors: Rutanachai Thaipratum
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Free radicals are atoms or molecules with unpaired electrons. Many diseases are caused by free radicals. Normally, free radical formation is controlled naturally by various beneficial compounds known as antioxidants. Several analytical methods have been used for qualitative and quantitative determination of antioxidants, and each has its own specificity. This project aimed to evaluate antioxidant activity of ethanolic and aqueous extracts from the rice paddy herb (Limnophila aromatica (Lam.) Merr.) measured by DPPH and Hydroxyl radical scavenging method. The results showed that averaged antioxidant activity measured in ethanolic extract (µmol Ascorbic acid equivalent/g fresh mass) were 67.09± 4.99 and 15.55±4.82 as determined by DPPH and Hydroxyl radical scavenging activity assays, respectively. Averaged antioxidant activity measured in aqueous extract (µmol Ascorbic acid equivalent/g fresh mass) were 21.08±1.25 and 10.14±3.94 as determined by DPPH and Hydroxyl radical scavenging activity assays respectively.
Keywords: Free radical, antioxidant, rice paddy herb, Limnophila aromatica (Lam.) Merr.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2180121 Ethanol and Biomass Production from Spent Sulfite Liquor by Filamentous Fungi
Authors: M. T. Asadollahzadeh, A. Ghasemian, A. R. Saraeian, H. Resalati, P. R. Lennartsson, M. J. Taherzadeh
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Since filamentous fungi are capable of assimilating several types of sugars (hexoses and pentoses), they are potential candidates for bioconversion of spent sulfite liquor (SSL). Three filamentous fungi such as Aspergillus oryzae, Mucor indicus, and Rhizopus oryzae were investigated in this work. The SSL was diluted in order to obtain concentrations of 50, 60, 70, 80, and 90% and supplemented with two types of nutrients. The results from cultivations in shake flask showed that A. oryzae and M. indicus were not able to grow in pure SSL and SSL90% while R. oryzae could grow only in SSL50% and SSL60%. Cultivation with A. oryzae resulted in the highest yield of produced fungal biomass, while R. oryzae cultivation resulted in the lowest fungal biomass yield. Although, the mediums containing yeast extract, (NH4)2SO4, KH2PO4, CaCl2∙2H2O, and MgSO4∙7H2O as nutrients supplementations produced higher fungal biomass compared to the mediums containing NH4H2PO4 and ammonia, but there was no significant difference between two types of nutrients in terms of sugars and acetic acid consumption rate. The sugars consumption in M. indicus cultivation was faster than A. oryzae and R. oryzae cultivation. Acetic acid present in SSL was completely consumed during cultivation of all fungi. M. indicus was the best and fastest ethanol producer from SSL among the fungi examined, when yeast extract and salts were used as nutrients supplementations. Furthermore, no further improvement in ethanol concentration and rate of sugars consumption was obtained in medium supplemented with NH4H2PO4 and ammonia compared to medium containing yeast extract, (NH4)2SO4, KH2PO4, CaCl2∙2H2O, and MgSO4∙7H2O. On the other hand, the higher dilution of SSL resulted in a better fermentability, and better consumption of sugars and acetic acid.
Keywords: Ethanol, filamentous fungi, fungal biomass, spent sulfite liquor.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1479120 Fermentation of Xylose and Glucose Mixture in Intensified Reactors by Scheffersomyces stipitis to Produce Ethanol
Authors: S. C. Santos, S. R. Dionísio, A. L. D. De Andrade, L. R. Roque, A. C. Da Costa, J. L. Ienczak
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In this work, two fermentations at different temperatures (25 and 30ºC), with cell recycling, were accomplished to produce ethanol, using a mix of commercial substrates, xylose (70%) and glucose (30%), as organic source for Scheffersomyces stipitis. Five consecutive fermentations of 80 g L-1 (1º, 2º and 3º recycles), 96 g L-1 (4º recycle) and 120 g L-1 (5º recycle)reduced sugars led to a final maximum ethanol concentration of 17.2 and 34.5 g L-1, at 25 and 30ºC, respectively. Glucose was the preferred substrate; moreover xylose startup degradation was initiated after a remaining glucose presence in the medium. Results showed that yeast acid treatment, performed before each cycle, provided improvements on cell viability, accompanied by ethanol productivity of 2.16 g L-1 h- 1 at 30ºC. A maximum 36% of xylose was retained in the fermentation medium and after five-cycle fermentation an ethanol yield of 0.43 g ethanol/g sugars was observed. S. stipitis fermentation capacity and tolerance showed better results at 30ºC with 83.4% of theoretical yield referenced on initial biomass.Keywords: 5-carbon sugar, cell recycling fermenter, mixed sugars, xylose-fermenting yeast.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2758119 Pleurotus sajor-caju (PSC) Improves Nutrient Contents and Maintains Sensory Properties of Carbohydrate-based Products
Authors: W. I. Wan Rosli, M. S. Aishah
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The grey oyster mushroom, Pleurotus sajor-caju (PSC), is a common edible mushroom and is now grown commercially around the world for food. This fungus has been broadly used as food or food ingredients in various food products for a long time. To enhance the nutritional quality and sensory attributes of bakery-based products, PSC powder is used in the present study to partially replace wheat flour in baked product formulations. The nutrient content and sensory properties of rice-porridge and unleavened bread (paratha) incorporated with various levels of PSC powder were studied. These food items were formulated with either 0%, 2%, 4% or 6% of PSC powder. Results show PSC powder recorded β-glucan at 3.57g/100g. In sensory evaluation, consumers gave higher score to both rice-porridge and paratha bread containing 2-4% PSC compared to those that are not added with PSC powder. The paratha containing 4% PSC powder can be formulated with the intention in improving overall acceptability of paratha bread. Meanwhile, for rice-porridge, consumers prefer the formulated product added with 4% PSC powder. In conclusion, the addition of PSC powder to partially wheat flour can be recommended for the purpose of enhancing nutritional composition and maintaining the acceptability of carbohydrate-based products.Keywords: Pleurotus sajor-caju (PSC), nutrient contents, sensory evaluation
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2469118 Preliminary Investigation on Combustion Characteristics of Rice Husk in FBC
Authors: W. Permchart, S. Tanatvanit
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The experimental results on combustion of rice husk in a conical fluidized bed combustor (referred to as the conical FBC) using silica sand as the bed material are presented in this paper. The effects of excess combustion air and combustor loading as well as the sand bed height on the combustion pattern in FBC were investigated. Temperatures and gas concentrations (CO and NO) along over the combustor height as well as in the flue gas downstream from the ash collecting cyclone were measured. The results showed that the axial temperature profiles in FBC were explicitly affected by the combustor loading whereas the excess air and bed height were found to have minor influences on the temperature pattern. Meanwhile, the combustor loading and the excess air significantly affected the axial CO and NO concentration profiles; however, these profiles were almost independent of the bed height. The combustion and thermal efficiencies for this FBC were quantified for different operating conditions.Keywords: Temperature, Combustor loading, Excess air, Bed height.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1631117 Investigation of Compressive Strength of Slag-Based Geopolymer Concrete Incorporated with Rice Husk Ash Using 12M Alkaline Activator
Authors: Festus A. Olutoge, Ahmed A. Akintunde, Anuoluwapo S. Kolade, Aaron A. Chadee, Jovanca Smith
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Geopolymer concrete's (GPC) compressive strength was investigated. The GPC was incorporated with rice husk ash (RHA) and ground granulated blast furnace slag (GGBFS), which may have potential in the construction industry to replace Portland limestone cement (PLC) concrete. The sustainable construction binders used were GGBFS and RHA, and a solution of sodium hydroxide (NaOH) and sodium silicate gel (Na2SiO3) was used as the 12-molar alkaline activator. Five GPC mixes comprising fine aggregates, coarse aggregates, GGBS, and RHA, and the alkaline solution in the ratio 2: 2.5: 1: 0.5, respectively, were prepared to achieve grade 40 concrete, and PLC was substituted with GGBFS and RHA in the ratios of 0:100, 25:75, 50:50, 75:25, and 100:0. A control mix was also prepared which comprised of 100% water and 100% PLC as the cementitious material. The GPC mixes were thermally cured at 60-80 ºC in an oven for approximately 24 h. After curing for 7 and 28 days, the compressive strength test results of the hardened GPC samples showed that GPC-Mix #3, comprising 50% GGBFS and 50% RHA, was the most efficient geopolymer mix. The mix had compressive strengths of 35.71 MPa and 47.26 MPa, 19.87% and 8.69% higher than the PLC concrete samples, which had 29.79 MPa and 43.48 MPa after 7 and 28 days, respectively. Therefore, GPC containing GGBFS incorporated with RHA is an efficient method of decreasing the use of PLC in conventional concrete production and reducing the high amounts of CO2 emitted into the atmosphere in the construction industry.
Keywords: Alkaline solution, cementitious material, geopolymer concrete, ground granulated blast furnace slag, rice husk ash.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 190116 Heating and Cooling Scenario of Blended Concrete Subjected to 780 Degrees Celsius
Authors: J. E. Oti, J. M. Kinuthia, R. Robinson, P. Davies
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In this study, the Compressive strength of concretes made with Ground Granulated Blast furnace Slag (GGBS), Pulverised Fuel Ash (PFA), Rice Husk Ash (RHA) and Waste Glass Powder (WGP) after they were exposed 7800C (exposure duration of around 60 minutes) and then allowed to cool down gradually in the furnace for about 280 minutes at water binder ratio of 0.50 was investigated. GGBS, PFA, RHA and WGP were used to replace up to 20% Portland cement in the control concrete. Test for the determination of workability, compressive strength and tensile splitting strength of the concretes were carried out and the results were compared with control concrete. The test results showed that the compressive strength decreased by an average of around 30% after the concretes were exposed to the heating and cooling scenario.
Keywords: Pulverised Fuel Ash, Rice Husk Ash, heating and cooling, concrete.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1784115 Physical and Thermo-Physical Properties of High Strength Concrete Containing Raw Rice Husk after High Temperature Effect
Authors: B. Akturk, N. Yuzer, N. Kabay
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High temperature is one of the most detrimental effects that cause important changes in concrete’s mechanical, physical, and thermo-physical properties. As a result of these changes, especially high strength concrete (HSC), may exhibit damages such as cracks and spallings. To overcome this problem, incorporating polymer fibers such as polypropylene (PP) in concrete is a very well-known method. In this study, using RRH, as a sustainable material, instead of PP fiber in HSC to prevent spallings and improve physical and thermo-physical properties were investigated. Therefore, seven HSC mixtures with 0.25 water to binder ratio were prepared incorporating silica fume and blast furnace slag. PP and RRH were used at 0.2-0.5% and 0.5-3% by weight of cement, respectively. All specimens were subjected to high temperatures (20 (control), 300, 600 and 900˚C) with a heating rate of 2.5˚C/min and after cooling, residual physical and thermo-physical properties were determined.
Keywords: High temperature, high strength concrete, polypropylene fiber, raw rice husk, thermo-physical properties.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2166