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Statistical Screening of Medium Components on Ethanol Production from Cashew Apple Juice using Saccharomyces diasticus

Authors: Karuppaiya Maruthai, Viruthagiri Thangavelu, Manikandan Kanagasabai

Abstract:

In the present study, effect of critical medium components (a total of fifteen components) on ethanol production from waste cashew apple juice (CAJ) using yeast Saccharomyces diasticus was studied. A statistical response surface methodology (RSM) based Plackett-Burman Design (PBD) was used for the design of experiments. The design contains a total of 32 experimental trails. The effect of medium components on ethanol was studied at two different levels such as low concentration level (-) and high concentration levels (+). The dependent variables selected in this study were ethanol concentration (g/L) and cellmass concentration (g/L). Data obtained from RSM on ethanol production were subjected to analysis of variance (ANOVA). In general, initial substrate concentration significantly influenced the microbial growth and product formation. Of the medium components evaluated, CAJ concentration, yeast extract, (NH4)2SO4, and malt extract showed significant effect on ethanol fermentation. A second-order polynomial model was used to predict the experimental data and the model fitted the data with a high correlation coefficient (R2 > 0.98). Maximum ethanol (15.3 g/L) and biomass (6.4 g/L) concentrations were obtained at the optimum medium composition and at optimum condition (temperature-30°C; initial pH-6.8) after 72 h fermentation using S.diasticus.

Keywords: Fermentation, Yeast, Ethanol, response surface methodology, cashew apple juice

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1326785

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References:


[1] L.R.Lynd, Cushman, J. H., Nichols, R. J., and Wyman, C. E. (1991) "Fuel Ethanol from Cellulosic Biomass", Science 251, 1318-1323.
[2] C.E.Wyman, and Goodman, B. J. (1993) "Biotechnology for Production of Fuels, Chemicals, and Materials from Biomass", Appl Biochem Biotech 39, 41-59.
[3] L.Lynd, Greene, N., Dale, B., Laser, M., Lashof, D., Wang, M., and Wyman, C. (2006) "Energy returns on ethanol production", Science 312, 1746-1747.
[4] K.Grohmann, Himmel, M. E., Hinman, N., and Wyman, C. E. (1990) "Production of Fuel Ethanol by Enzymatic-Hydrolysis of Cellulosic Biomass", Abstr Pap Am Chem S 200, 52-Cell.
[5] A.Aristidou, and Penttila, M. (2000) "Metabolic engineering applications to renewable resource utilization", Curr Opin Biotech 11, 187-198.
[6] C.E.Wyman, (2010) "Introduction Overview: World Energy Resources and the Need for Biomass for Energy and Lower Fossil Carbon Dioxide Emissions", Biotech Agr Forest 66, 3-23.
[7] J.Zaldivar, Nielsen, J., and Olsson, L. (2001) "Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration", Appl Microbiol Biot 56, 17-34.
[8] M.E.Silva, Torres, A. B., Silva, W. B., Silva, F. L. H., and Swarnakar, R. (2007) "Cashew wine vinegar production: Alcoholic and acetic fermentation", Braz J Chem Eng 24, 163-169.
[9] T.L.Honorato, Rabelo, M. C., Goncalves, L. R. B., Pinto, G. A. S., and Rodrigues, S. (2007) "Fermentation of cashew apple juice to produce high added value products", World J Microb Biot 23, 1409-1415.
[10] P.Wimalsiri, Sinnatamby, A., Samaranayake, S., and Samarasinghe, C. R. (1971) "Cashew apple wine", Industrial Prospect Report 44, 15-19.
[11] L.Kinh, Do, V., and D., P. (1996) "Chemical composition of cashew apple and cashew waste ensiled with poultry litter", Livestock Research in Rural Development 9, 1-8.
[12] J.N.Nigam, (2000) "Continuous ethanol production from pineapple cannery waste using immobilized yeast cells", J Biotechnol 80, 189-193.
[13] K.Szambelan, Nowak, J., and Czarnecki, Z. (2004) "Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers", Biotechnol Lett 26, 845-848.
[14] R.Balusu, Paduru, R. M. R., Seenayya, G., and Reddy, G. (2004) "Production of ethanol from cellulosic biomass by Clostridium thermocellum SS19 in submerged fermentation - Screening of nutrients using Plackett-Burman design", Appl Biochem Biotech 117, 133-141.
[15] D.Bas, and Boyaci, I. H. (2007) "Modeling and optimization I: Usability of response surface methodology", J Food Eng 78, 836-845.
[16] O.Jargalsaikhan, and Saracoglu, N. (2009) "Application of Experimental Design Method for Ethanol Production by Fermentation of Sunflower Seed Hull Hydrolysate Using Pichia stipitis NRRL-124", Chem Eng Commun 196, 93-103.
[17] K.J.Lee, and Rogers, P. L. (1983) "The fermentation kinetics of ethanol produciton by Zymomonas mobilis", Journal of Chemical Engineering 27, 31-38.
[18] D.C.P.Campos, Santos, A. S., Wolkoff, D. B., Matta, V. M., Cabral, L. M. C., and Couri, S. (2002) "Cashew apple juice stabilization by microfiltration", Desalination 148, 61-65.
[19] M.Karuppaiya, Sasikumar, E., Viruthagiri, T., and Vijayagopal, V. (2009) "Optimization of Process Conditions Using Response Surface Methodology (Rsm) for Ethanol Production from Waste Cashew Apple Juice by Zymomonas Mobilis", Chem Eng Commun 196, 1425-1435.
[20] K.Manikandan, and Viruthagiri, T. (2009) "Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic Aspergillus niger and thermotolerant Kluyveromyces marxianus", Frontiers of Chemical Engineering China 3, 240-249.
[21] J.C.Anthony, (1984) "Gas chromatographic determination of ethanol in beer", Analytical Chemistry 64, 192-193.
[22] G.L.Miller, (1959) "Use of dinitrosalicylic acid reagent for determination of total reducing sugar", Analytical Chemistry 31, 420-426.
[23] Y.Lin, and Tanaka, S. (2006) "Ethanol fermentation from biomass resources: current state and prospects", Appl Microbiol Biot 69, 627-642.
[24] S.Ozmihci, and Kargi, F. (2007) "Kinetics of batch ethanol fermentation of cheese-whey powder (CWP) solution as function of substrate and yeast concentrations", Bioresource Technol 98, 2978-2984.
[25] S.Ozmihci, and Kargi, F. (2007) "Effects of feed sugar concentration on continuous ethanol fermentation of cheese whey powder solution (CWP)", Enzyme Microb Tech 41, 876-880.
[26] B.L.Maiorella, Blanch, H. W., and Wilke, C. R. (1984) "Feed Component Inhibition in Ethanolic Fermentation by Saccharomyces-Cerevisiae", Biotechnol Bioeng 26, 1155-1166.
[27] J.R.M.Almeida, Modig, T., Petersson, A., Hahn-Hagerdal, B., Liden, G., and Gorwa-Grauslund, M. F. (2007) "Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae", J Chem Technol Biot 82, 340-349.