Evaluation of Antioxidant Activities of Rice Paddy Herb (Limnophila aromatica (Lam.) Merr.)

Authors: Rutanachai Thaipratum

Abstract:

Free radicals are atoms or molecules with unpaired electrons. Many diseases are caused by free radicals. Normally, free radical formation is controlled naturally by various beneficial compounds known as antioxidants. Several analytical methods have been used for qualitative and quantitative determination of antioxidants, and each has its own specificity. This project aimed to evaluate antioxidant activity of ethanolic and aqueous extracts from the rice paddy herb (Limnophila aromatica (Lam.) Merr.) measured by DPPH and Hydroxyl radical scavenging method. The results showed that averaged antioxidant activity measured in ethanolic extract (µmol Ascorbic acid equivalent/g fresh mass) were 67.09± 4.99 and 15.55±4.82 as determined by DPPH and Hydroxyl radical scavenging activity assays, respectively. Averaged antioxidant activity measured in aqueous extract (µmol Ascorbic acid equivalent/g fresh mass) were 21.08±1.25 and 10.14±3.94 as determined by DPPH and Hydroxyl radical scavenging activity assays respectively.

Keywords: Free radical, antioxidant, rice paddy herb, Limnophila aromatica (Lam.) Merr.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1092838

References:

[1] Q.D. Do, A.E. Angkawijaya, P.L. Tran-Nguyen, L.H. Huynh, F.E. Soetaredjo, S Ismadji, Y-H Ju."Effect of extraction solvent on total phenol content, total flavonoids content, and antioxidant activity of limnophila aromatica”, Journal of food and drug analysis., 2013, pp. 1-7.
[2] H-Y Luo, B Wang, C-G Yu, Y-L Qu, C-L Su."Evaluation of antioxidant activities of five selected brown seaweeds from Chin”, Journal of Medicinal Plants Research., 2010, pp. 2557-2565.
[3] F Liu, V.E.C. Ooi, S.T. Chang."Free radical scavenging activities of mushroom polysaccharide extracts”, Life Sciences., 1997, pp. 763-771.
[4] B Wang, Z-R Li, C-F Chi, Q-H Zhang, H-Y Luo."Preparation and evaluation of antioxidant peptides from ethanol-soluble proteins hydrolysate of Sphyrna lewini muscle”, Peptides., 2012, pp. 240–250.
[5] Z Youwei, Z Jinlian, P Yonghong."A comparative study on the free radical scavenging activities of some fresh flowers in southern China”, LWT - Food Science and Technology., 2008, pp. 1586-1591.