Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30135
Biodegradation of Lignocellulosic Residues of Water Hyacinth (Eichhornia crassipes) and Response Surface Methodological Approach to Optimize Bioethanol Production Using Fermenting Yeast Pachysolen tannophilus NRRL Y-2460

Authors: A. Manivannan, R. T. Narendhirakannan

Abstract:

The objective of this research was to investigate biodegradation of water hyacinth (Eichhornia crassipes) to produce bioethanol using dilute-acid pretreatment (1% sulfuric acid) results in high hemicellulose decomposition and using yeast (Pachysolen tannophilus) as bioethanol producing strain. A maximum ethanol yield of 1.14g/L with coefficient, 0.24g g-1; productivity, 0.015g l-1h-1 was comparable to predicted value 32.05g/L obtained by Central Composite Design (CCD). Maximum ethanol yield coefficient was comparable to those obtained through enzymatic saccharification and fermentation of acid hydrolysate using fully equipped fermentor. Although maximum ethanol concentration was low in lab scale, the improvement of lignocellulosic ethanol yield is necessary for large scale production.

Keywords: Acid hydrolysis, Biodegradation, Hemicellulose, Pachysolen tannophilus, Water hyacinth.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1091974

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1521

References:


[1] J.A. Asenjo, W.H. Sun, and J.L. Spencer, "Optimization of batch process involving simultaneous enzyme and microbial reactions,” Biotech. Bioeng., Vol. 3, 1991, pp. 1087-1094.
[2] A.Demirbas, "Producing and using bioethanol as an automotive fuel,” Energy Sources, Part B. Vol. 2, 2007, pp. 391–401.
[3] A.Demirbas, "Biofuels for internal combustion engines” Energy Education Science and Technology, Part A. Vol. 22, 2009, pp. 117–132.
[4] S.V. Mohan, V.L. Babu, and P.N. Sarma, "Effect of various pretreatment methods on anaerobic mixed microflora to enhance biohydrogen production utilizing dairy wastewater as substrate,” Bioresour. Technol., Vol. 99, 2008, pp. 59–67.
[5] S.M. Mohanty, S. Behera, M.R. Swain, and R.C. Ray, "Bioethanol production from mahula (Madhuca latifolia L.) flowers by solid-state fermentation,” App. Ene., Vol. 86, 2009, pp. 640–644.
[6] L.R. Lynd, J.H. Cushman, R.J. Nichols, and C.E.Wyman, "Fuel ethanol from cellulosic biomass,” Sci., Vol. 251, 1991a, pp. 1318.
[7] M. Mahalakshmi, J. Angayarkanni, R. Rajendran, and R. Rajesh, "Bioconversion of cotton waste from textile mills to bioethanol by microbial saccharification and fermentation,” Ann. Biolo. Res., Vol. 2, no. 3, 2011, pp. 380-388.
[8] O.J. Sanchez, and C.A. Cardona, "Trends in biotechnological production of fuel ethanol from different feedstocks,” Bioresour. Technol., Vol. 99, 2008, pp. 5270–5295.
[9] A.Martinez, M.E. Rodriguez, S.W. York, J.F. Preston, and L.O. Ingram, "Effects of Ca(OH)2 treatments ("overliming") on the composition and toxicity of bagasse hemicellulose hydrolysates,” Biotech. Bioeng., Vol. 69, 2000, pp. 526-536.
[10] T.J. Eberts, R.H. Sample, M.R. Glick, and G.H. Ellis, "A simplified, colorimetric micromethod for xylose in serum or urine with phloroglucinol,” Clin. Chem., Vol. 25, 1979, pp. 1440-1443.
[11] S.L. Johnson, M. Bliss, M. Mayersohn, and K.Conrad, "A Phloroglucinol-based colorimetry of xylose in plasma and urine compared with a specific gaschromatographic procedure” Clin. Chem., Vol. 30, 1984, pp. 1571-1574.
[12] Bennett, "Spectrophotometric acid dichromate method for the determination of ethyl alcohol” The Am. J. Med. Technol., Vol. 37, 1971, pp. 217-220.
[13] G.J. Pilone, "Determination of ethanol in wine by titrimetric and spectrophotometric dichromate methods: collaborative study,” J. Assoc. Off. Analy. Chemist. Vol. 68, 1985, pp. 188-190.
[14] M. Ackerson, M. Ziobro, and J.L. Gaddy, "Two-stage acid hydrolysis of biomass,” Biotechnol. Bioengin. Sympo., Vol. 11, 1981, pp. 103-112.
[15] K. Grohman, R. Torget, and M. Himmel, "Optimization of dilute acid pretreatment of biomass, Biotechnol. Bioengin. Sympo., Vol. 15, 1985, pp. 59-80.
[16] O.P. Ward, and A. Singh, "Bioethanol technology: developments and perspectives,” Advances in Appl. Microbio., Vol. 51, 2002, pp. 53–80.
[17] O.P. Ward, A. Singh, R.C. Ray, "Production of renewable energy from agricultural and horticultural substrates and wastes” Microbial biotechnology in horticulture. Enfield New Hampshire,Science Publishers, pp. 517–518, 2006.
[18] R. Muralidhar, S.N. Gummadi, V.V. Dasu, and T. Panda, "Statistical analysis on some critical parameters affecting the formation of protoplasts from the mycelium of Penicillium griseofulvum,” Biochem. Eng. J., Vol. 16 no. 3, 2003, pp. 229-235.
[19] M.R. Wilkins, W.W. Widmer, and K. Grohmann, "Simultaneous saccharification and fermentation of citrus peel waste by Saccharomyces cerevisiae to produce ethanol,” Pro. Biochem., Vol. 42 no. 12, 2007, pp. 1614-1619.
[20] M. Phisalaphong, N. Srirattana, and W. Tanthapanichakoon, "Mathematical modeling to investigate temperature effect on kinetic parameters of ethanol fermentation,” Biochem. Eng. J., Vol. 28, no. 1, 2005, pp. 36-43.
[21] A.Manivannan, P. Hepsibha jeyarani, R.T. Narendhirakannan, "Enhanced Acid Hydrolysis for Bioethanol Production from Water Hyacinth (Eichhornia crassipes) Using Fermentating Yeast Candida intermedia NRRL Y-981,” J. Sci. Ind. Res., Vol. 71, 2012, pp. 51-56.
[22] A.E. Asli, E.Boles, C.P. Hollenberg, and M. Errami, "Conversion of xylose to ethanol by a novel phenoltolerant strain of Enterobacteriaceae isolated from olive mill wastewater,” Biotechnol. Lett., Vol. 24, 2002, pp. 1101-1105.
[23] M. Abraham, and G.M. Kurup, "Bioconversion of tapioca (Manihot esculenta) waste and water hyacinth (Eichhornia crassipes)-Influence of various physicochemical factors,” J. Fermen. Bioengin., Vol. 82, 1996, pp. 259-263.