Search results for: reticence scale
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1375

Search results for: reticence scale

925 Decentralised Edge Authentication in the Industrial Enterprise IoT Space

Authors: C. P. Autry, A.W. Roscoe

Abstract:

Authentication protocols based on public key infrastructure (PKI) and trusted third party (TTP) are no longer adequate for industrial scale IoT networks thanks to issues such as low compute and power availability, the use of widely distributed and commercial off-the-shelf (COTS) systems, and the increasingly sophisticated attackers and attacks we now have to counter. For example, there is increasing concern about nation-state-based interference and future quantum computing capability. We have examined this space from first principles and have developed several approaches to group and point-to-point authentication for IoT that do not depend on the use of a centralised client-server model. We emphasise the use of quantum resistant primitives such as strong cryptographic hashing and the use multi-factor authentication.

Keywords: Authentication, enterprise IoT cybersecurity, public key infrastructure, trusted third party.

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924 Integration of Microarray Data into a Genome-Scale Metabolic Model to Study Flux Distribution after Gene Knockout

Authors: Mona Heydari, Ehsan Motamedian, Seyed Abbas Shojaosadati

Abstract:

Prediction of perturbations after genetic manipulation (especially gene knockout) is one of the important challenges in systems biology. In this paper, a new algorithm is introduced that integrates microarray data into the metabolic model. The algorithm was used to study the change in the cell phenotype after knockout of Gss gene in Escherichia coli BW25113. Algorithm implementation indicated that gene deletion resulted in more activation of the metabolic network. Growth yield was more and less regulating gene were identified for mutant in comparison with the wild-type strain.

Keywords: Metabolic network, gene knockout, flux balance analysis, microarray data, integration.

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923 An Advanced Method for Speech Recognition

Authors: Meysam Mohamad pour, Fardad Farokhi

Abstract:

In this paper in consideration of each available techniques deficiencies for speech recognition, an advanced method is presented that-s able to classify speech signals with the high accuracy (98%) at the minimum time. In the presented method, first, the recorded signal is preprocessed that this section includes denoising with Mels Frequency Cepstral Analysis and feature extraction using discrete wavelet transform (DWT) coefficients; Then these features are fed to Multilayer Perceptron (MLP) network for classification. Finally, after training of neural network effective features are selected with UTA algorithm.

Keywords: Multilayer perceptron (MLP) neural network, Discrete Wavelet Transform (DWT) , Mels Scale Frequency Filter , UTA algorithm.

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922 A New Efficient Scalable BIST Full Adder using Polymorphic Gates

Authors: M. Mashayekhi, H. H. Ardakani, A. Omidian

Abstract:

Among various testing methodologies, Built-in Self- Test (BIST) is recognized as a low cost, effective paradigm. Also, full adders are one of the basic building blocks of most arithmetic circuits in all processing units. In this paper, an optimized testable 2- bit full adder as a test building block is proposed. Then, a BIST procedure is introduced to scale up the building block and to generate a self testable n-bit full adders. The target design can achieve 100% fault coverage using insignificant amount of hardware redundancy. Moreover, Overall test time is reduced by utilizing polymorphic gates and also by testing full adder building blocks in parallel.

Keywords: BIST, Full Adder, Polymorphic Gate

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921 Removal of Heavy Metals from Rainwater in Batch Reactors with Sulphate Reducing Bacteria (SRB)

Authors: Abdulsalam I. Rafida

Abstract:

The main objective of this research was to investigate the biosorption capacity for biofilms of sulphate reducing bacteria (SRB) to remove heavy metals, such as Zn, Pb and Cd from rainwater using laboratory-scale reactors containing mixed support media. Evidence showed that biosorption had contributed to removal of heavy metals including Zn, Pb and Cd in presence of SRB and SRB were also found in the aqueous samples from reactors. However, the SRB and specific families (Desulfobacteriaceae and Desulfovibrionaceae) were found mainly in the biomass samples taken from all reactors at the end of the experiment. EDX-analysis of reactor solids at end of experiment showed that heavy metals Zn, Pb and Cd had also accumulated in these precipitates.

Keywords: Sulphate reducing bacteria (SRB), biosorption capacity.

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920 Information Theoretical Analysis of Neural Spiking Activity with Temperature Modulation

Authors: Young-Seok Choi

Abstract:

This work assesses the cortical and the sub-cortical neural activity recorded from rodents using entropy and mutual information based approaches to study how hypothermia affects neural activity. By applying the multi-scale entropy and Shannon entropy, we quantify the degree of the regularity embedded in the cortical and sub-cortical neurons and characterize the dependency of entropy of these regions on temperature. We study also the degree of the mutual information on thalamocortical pathway depending on temperature. The latter is most likely an indicator of coupling between these highly connected structures in response to temperature manipulation leading to arousal after global cerebral ischemia.

Keywords: Spiking activity, entropy, mutual information, temperature modulation.

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919 Identification of Impact of Electromagnetic Fields at Low and High Frequency on Human Body

Authors: P. Sowa

Abstract:

The article reviews the current state of large-scale studies about the impact of electromagnetic field on natural environment. The scenario of investigations – simulation of natural conditions at the workplace, taking into consideration the influence both low and high frequency electromagnetic fields is shown.The biological effects of low and high frequency electromagnetic fields are below presented. Results of investigation with animals are shown. The norms and regulations concerning the levels of electromagnetic field intensity are reviewed.

Keywords: Electromagnetic field and environment, biological effects of electric field on human body, simulation of natural condition at workplace

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918 Circular Raft Footings Strengthened by Stone Columns under Static Loads

Authors: R. Ziaie Moayed, B. Mohammadi-Haji

Abstract:

Stone columns have been widely employed to improve the load-settlement characteristics of soft soils. The results of two small scale displacement control loading tests on stone columns were used in order to validate numerical finite element simulations. Additionally, a series of numerical calculations of static loading have been performed on strengthened raft footing to investigate the effects of using stone columns on bearing capacity of footings. The bearing capacity of single and group of stone columns under static loading compares with unimproved ground.

Keywords: Circular raft footing, numerical analysis, validation, vertically encased stone column.

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917 Intrinsic Kinetics of Methanol Dehydration over Al2O3 Catalyst

Authors: Liang Zhang, Hai-Tao Zhang, W ei-Yong Ying, Ding-Ye Fang

Abstract:

Dehydration of methanol to dimethyl ether (DME) over a commercial Al2O3 catalyst was studied in an isothermal integral fixed bed reactor. The experiments were performed on the temperature interval 513-613 K, liquid hourly space velocity (LHSV) of 0.9-2.1h-1, pressures between 0.1 and 1.0 MPa. The effect of different operation conditions on the dehydration of methanol was investigated in a laboratory scale experiment. A new intrinsic kinetics equation based on the mechanism of Langmuir-Hinshelwood dissociation adsorption was developed for the dehydration reaction by fitting the expressions to the experimental data. An activation energy of 67.21 kJ/mol was obtained for the catalyst with the best performance. Statistic test showed that this new intrinsic kinetics equation was acceptable.

Keywords: catalyst, dimethyl ether, intrinsic kinetics, methanol

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916 Experimental Study on the Floor Vibration Evaluation of Concrete Slab for Existing Buildings

Authors: Yong-Taeg Lee, Jun-Ho Na, Seung-Hun Kim, Seong-Uk Hong

Abstract:

Damages from noise and vibration are increasing every year, most of which are noises between floors in deteriorated building caused by floor impact sound. In this study, the concrete slab measured vibration impact sound for evaluation floor vibration of deteriorated buildings that fails to satisfy with the minimum thickness. In this experimental study, the vibration scale by impact sound was calibrated and compared with ISO and AIJ standard for vibration. The results show that vibration in slab with thickness used in existing building reach human perception levels.

Keywords: Vibration, Frequency, Accelerometer, Concrete slab.

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915 Understanding and Predicting Foam in Anaerobic Digester

Authors: I. R. Kanu, T. J. Aspray, A. J. Adeloye

Abstract:

As a result of the ambiguity and complexity surrounding anaerobic digester foaming, efforts have been made by various researchers to understand the process of anaerobic digester foaming so as to proffer a solution that can be universally applied rather than site specific. All attempts ranging from experimental analysis to comparative review of other process has not fully explained the conditions and process of foaming in anaerobic digester. Studying the current available knowledge on foam formation and relating it to anaerobic digester process and operating condition, this piece of work presents a succinct and enhanced understanding of foaming in anaerobic digesters as well as introducing a simple method to identify the onset of anaerobic digester foaming based on analysis of historical data from a field scale system.

Keywords: Anaerobic digester, foam, biogas, surfactants, wastewater sludge.

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914 Optical Induction of 2D and 3D Photonic Lattices in Photorefractive Materials based on Talbot effect

Authors: A. Badalyan, R. Hovsepyan, V. Mekhitaryan, P. Mantashyan, R. Drampyan

Abstract:

In this paper we report the technique of optical induction of 2 and 3-dimensional (2D and 3D) photonic lattices in photorefractive materials based on diffraction grating self replication -Talbot effect. 1D and 2D different rotational symmery diffraction masks with the periods of few tens micrometers and 532 nm cw laser beam were used in the experiments to form an intensity modulated light beam profile. A few hundred micrometric scale replications of mask generated intensity structures along the beam propagation axis were observed. Up to 20 high contrast replications were detected for 1D annular mask with 30

Keywords: Diffraction gratings, laser, photonic lattice, Talbot effect.

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913 A New SIR-based Model for Influenza Epidemic

Authors: Kang-Hung Yang, Jin-Yuan Hsu

Abstract:

In recent years, several severe large-scale influenza outbreaks happened in many countries, such as SARS in 2005 or H1N1 in 2009. Those influenza Epidemics have greatly impacts not only on people-s life and health, but medical systems in different countries. Although severe diseases are more experienced, they are not fully controlled. Governments have different policies to control the spreads of diseases. However, those policies have both positive and negative social or economical influence on people and society. Therefore, it is necessary and essential to develop an appropriate model for evaluations of policies. Consequently, a proper measure can be implemented to confront the diseases. The main goal of this study is to develop a SIR-based model for the further evaluations of the candidate policies during the influenza outbreaks.

Keywords: SIR, influenza, systems dynamic model, H1N1

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912 Solving 94-bit ECDLP with 70 Computers in Parallel

Authors: Shunsuke Miyoshi, Yasuyuki Nogami, Takuya Kusaka, Nariyoshi Yamai

Abstract:

Elliptic curve discrete logarithm problem(ECDLP) is one of problems on which the security of pairing-based cryptography is based. This paper considers Pollard’s rho method to evaluate the security of ECDLP on Barreto-Naehrig(BN) curve that is an efficient pairing-friendly curve. Some techniques are proposed to make the rho method efficient. Especially, the group structure on BN curve, distinguished point method, and Montgomery trick are well-known techniques. This paper applies these techniques and shows its optimization. According to the experimental results for which a large-scale parallel system with MySQL is applied, 94-bit ECDLP was solved about 28 hours by parallelizing 71 computers.

Keywords: Pollard’s rho method, BN curve, Montgomery multiplication.

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911 A Sliding Mesh Technique and Compressibility Correction Effects of Two-equation Turbulence Models for a Pintle-Perturbed Flow Analysis

Authors: J. Y. Heo, H. G. Sung

Abstract:

Numerical simulations have been performed for assessment of compressibility correction of two-equation turbulence models suitable for large scale separation flows perturbed by pintle strokes. In order to take into account pintle movement, a sliding mesh method was applied. The chamber pressure, mass flow rate, and thrust have been analyzed, and the response lag and sensitivity at the chamber and nozzle were estimated for a movable pintle. The nozzle performance for pintle reciprocating as its insertion and extraction processes, were analyzed to better understand the dynamic performance of the pintle nozzle.

Keywords: Pintle, sliding mesh, turbulent model, compressibility correction.

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910 Extension of the Client-Centric Approach under Small Buffer Space

Authors: Hsiang-Fu Yu, Yu-Chan Hsu, Chun Fang, Hao-Yun Yang

Abstract:

Periodic broadcast is a cost-effective solution for large-scale distribution of popular videos because this approach guarantees constant worst service latency, regardless of the number of video requests. An essential periodic broadcast method is the client-centric approach (CCA), which allows clients to use smaller receiving bandwidth to download broadcast data. An enhanced version, namely CCA++, was proposed to yield a shorter waiting time. This work further improves CCA++ in reducing client buffer requirements. The new scheme decreases the buffer requirements by as much as 52% when compared to CCA++. This study also provides an analytical evaluation to demonstrate the performance advantage, as compared with particular schemes.

Keywords: Periodic broadcast, client-centric approach, buffer space, multimedia communications.

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909 Stability of Interconnected Systems under Structural Perturbation: Decomposition-Aggregation Approach

Authors: M. Kidouche, H. Habbi, M. Zelmat

Abstract:

In this paper, the decomposition-aggregation method is used to carry out connective stability criteria for general linear composite system via aggregation. The large scale system is decomposed into a number of subsystems. By associating directed graphs with dynamic systems in an essential way, we define the relation between system structure and stability in the sense of Lyapunov. The stability criteria is then associated with the stability and system matrices of subsystems as well as those interconnected terms among subsystems using the concepts of vector differential inequalities and vector Lyapunov functions. Then, we show that the stability of each subsystem and stability of the aggregate model imply connective stability of the overall system. An example is reported, showing the efficiency of the proposed technique.

Keywords: Composite system, Connective stability, Lyapunovfunctions.

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908 14-Bit 1MS/s Cyclic-Pipelined ADC

Authors: S. Saisundar, Shan Jiang, Kevin T. C. Chai, David Nuttman, Minkyu Je

Abstract:

This paper presents a 14-bit cyclic-pipelined Analog to digital converter (ADC) running at 1 MS/s. The architecture is based on a 1.5-bit per stage structure utilizing digital correction for each stage. The ADC consists of two 1.5-bit stages, one shift register delay line, and digital error correction logic. Inside each 1.5-bit stage, there is one gain-boosting op-amp and two comparators. The ADC was implemented in 0.18µm CMOS process and the design has an area of approximately 0.2 mm2. The ADC has a differential input range of 1.2 Vpp. The circuit has an average power consumption of 3.5mA with 10MHz sampling clocks. The post-layout simulations of the design satisfy 12-bit SNDR with a full-scale sinusoid input.


Keywords: Analog to digital converter, cyclic, gain-boosting, pipelined.

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907 Multiscale Analysis and Change Detection Based on a Contrario Approach

Authors: F.Katlane, M.S.Naceur, M.A.Loghmari

Abstract:

Automatic methods of detecting changes through satellite imaging are the object of growing interest, especially beca²use of numerous applications linked to analysis of the Earth’s surface or the environment (monitoring vegetation, updating maps, risk management, etc...). This work implemented spatial analysis techniques by using images with different spatial and spectral resolutions on different dates. The work was based on the principle of control charts in order to set the upper and lower limits beyond which a change would be noted. Later, the a contrario approach was used. This was done by testing different thresholds for which the difference calculated between two pixels was significant. Finally, labeled images were considered, giving a particularly low difference which meant that the number of “false changes” could be estimated according to a given limit.

Keywords: multi-scale, a contrario approach, significantthresholds, change detection.

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906 Visual Cryptography by Random Grids with Identifiable Shares

Authors: Ran-Zan Wang, Yao-Ting Lee

Abstract:

This paper proposes a visual cryptography by random grids scheme with identifiable shares. The method encodes an image O in two shares that exhibits the following features: (1) each generated share has the same scale as O, (2) any share singly has noise-like appearance that reveals no secret information on O, (3) the secrets can be revealed by superimposing the two shares, (4) folding a share up can disclose some identification patterns, and (5) both of the secret information and the designated identification patterns are recognized by naked eye without any computation. The property to show up identification patterns on folded shares establishes a simple and friendly interface for users to manage the numerous shares created by VC schemes.

Keywords: Image Encryption, Image Sharing, Secret Sharing, Visual Cryptography.

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905 Color Constancy using Superpixel

Authors: Xingsheng Yuan, Zhengzhi Wang

Abstract:

Color constancy algorithms are generally based on the simplified assumption about the spectral distribution or the reflection attributes of the scene surface. However, in reality, these assumptions are too restrictive. The methodology is proposed to extend existing algorithm to applying color constancy locally to image patches rather than globally to the entire images. In this paper, a method based on low-level image features using superpixels is proposed. Superpixel segmentation partition an image into regions that are approximately uniform in size and shape. Instead of using entire pixel set for estimating the illuminant, only superpixels with the most valuable information are used. Based on large scale experiments on real-world scenes, it can be derived that the estimation is more accurate using superpixels than when using the entire image.

Keywords: color constancy, illuminant estimation, superpixel

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904 Authenticast: A Source Authentication Protocol for Multicast Flows and Streams

Authors: Yacine Challal, Abdelmadjid Bouabdallah

Abstract:

The lack of security obstructs a large scale de- ployment of the multicast communication model. There- fore, a host of research works have been achieved in order to deal with several issues relating to securing the multicast, such as confidentiality, authentication, non-repudiation, in- tegrity and access control. Many applications require au- thenticating the source of the received traffic, such as broadcasting stock quotes and videoconferencing and hence source authentication is a required component in the whole multicast security architecture. In this paper, we propose a new and efficient source au- thentication protocol which guarantees non-repudiation for multicast flows, and tolerates packet loss. We have simu- lated our protocol using NS-2, and the simulation results show that the protocol allows to achieve improvements over protocols fitting into the same category.

Keywords: Source Authentication, Non-repudiation, Multicast Security.

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903 Assessment of Multiscale Information for Short Physiological Time Series

Authors: Young-Seok Choi

Abstract:

This paper presents a multiscale information measure of Electroencephalogram (EEG) for analysis with a short data length. A multiscale extension of permutation entropy (MPE) is capable of fully reflecting the dynamical characteristics of EEG across different temporal scales. However, MPE yields an imprecise estimation due to coarse-grained procedure at large scales. We present an improved MPE measure to estimate entropy more accurately with a short time series. By computing entropies of all coarse-grained time series and averaging those at each scale, it leads to the modified MPE (MMPE) which provides an enhanced accuracy as compared to MPE. Simulation and experimental studies confirmed that MMPE has proved its capability over MPE in terms of accuracy.

Keywords: Multiscale entropy, permutation entropy, EEG, seizure.

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902 System Reduction by Eigen Permutation Algorithm and Improved Pade Approximations

Authors: Jay Singh, Kalyan Chatterjee, C. B. Vishwakarma

Abstract:

A mixed method by combining a Eigen algorithm and improved pade approximations is proposed for reducing the order of the large-scale dynamic systems. The most dominant Eigen value of both original and reduced order systems remain same in this method. The proposed method guarantees stability of the reduced model if the original high-order system is stable and is comparable in quality with the other well known existing order reduction methods. The superiority of the proposed method is shown through examples taken from the literature.

Keywords: Eigen algorithm, Order reduction, improved pade approximations, Stability, Transfer function.

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901 Regeneration of Spent Catalysts with Ozone

Authors: Jyh-Cherng Chen, Chang-Yong Liu

Abstract:

This study investigates the in-situ regeneration of deactivated Pt-Pd catalyst in a laboratory-scale catalysis reactor. Different regeneration conditions are tested and the activity and characteristics of regenerated catalysts are analyzed. Experimental results show that the conversion efficiencies of C3H6 by different regenerated Pt-Pd catalysts were significantly improved from 77%, 55% and 41% to 86%, 98% and 99%, respectively. The best regeneration conditions was 52ppm ozone, 500oC, and 10min. Regeneration temperature has more influences than ozone concentration and regeneration time. With the comparisons of characteristics of deactivated catalyst and regenerated catalyst, the major poison species (carbon, metals, chloride, and sulfate) on the spent catalysts can be effectively removed by ozone regeneration. 

Keywords: Catalyst, deactivated, ozone, regeneration.

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900 Slow Pyrolysis of Biowastes: Environmental, Exergetic, and Energetic Assessment

Authors: Daniela Zalazar-Garcia, Erick Torres, Germán Mazza

Abstract:

Slow pyrolysis of a pellet of pistachio waste was studied using a lab-scale stainless-steel reactor. Experiments were conducted at different heating rates (5, 10, and 15 K/min). A 3-E (environmental, exergetic, and energetic) analysis for the processing of 20 kg/h of biowaste was carried out. Experimental results showed that biochar and gas yields decreased with an increase in the heating rate (43% to 36% and 28% to 24%, respectively), while the bio-oil yield increased (29% to 40%). Finally, from the 3-E analysis and the experimental results, it can be suggested that an increase in the heating rate resulted in a higher pyrolysis exergetic efficiency (70%), due to an increase of the bio-oil yield with high-energy content.

Keywords: 3E assessment, biowaste pellet, life cycle assessment, slow pyrolysis.

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899 A Distributed Algorithm for Intrinsic Cluster Detection over Large Spatial Data

Authors: Sauravjyoti Sarmah, Rosy Das, Dhruba Kr. Bhattacharyya

Abstract:

Clustering algorithms help to understand the hidden information present in datasets. A dataset may contain intrinsic and nested clusters, the detection of which is of utmost importance. This paper presents a Distributed Grid-based Density Clustering algorithm capable of identifying arbitrary shaped embedded clusters as well as multi-density clusters over large spatial datasets. For handling massive datasets, we implemented our method using a 'sharednothing' architecture where multiple computers are interconnected over a network. Experimental results are reported to establish the superiority of the technique in terms of scale-up, speedup as well as cluster quality.

Keywords: Clustering, Density-based, Grid-based, Adaptive Grid.

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898 Potential of Agro-Waste Extracts as Supplements for the Continuous Bioremediation of Free Cyanide Contaminated Wastewater

Authors: Seteno K. O. Ntwampe, Bruno A. Q. Santos

Abstract:

Different agricultural waste peels were assessed for their suitability to be used as primary substrates for the bioremediation of free cyanide (CN-) by a cyanide-degrading fungus Aspergillus awamori isolated from cyanide containing wastewater. The bioremediated CN- concentration were in the range of 36 to 110 mg CN-/L, with Orange (C. sinensis) > Carrot (D. carota) > Onion (A. cepa) > Apple (M. pumila), being chosen as suitable substrates for large scale CN- degradation processes due to: 1) the high concentration of bioremediated CN-, 2) total reduced sugars released into solution to sustain the biocatalyst, and 3) minimal residual NH4- N concentration after fermentation. The bioremediation rate constants (k) were 0.017h-1 (0h < t < 24h), with improved bioremediation rates (0.02189h-1) observed after 24h. The averaged nitrilase activity was ~10 U/L.

Keywords: Agricultural waste, Bioremediation, Cyanide, Wastewater.

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897 Losses Analysis in TEP Considering Uncertainity in Demand by DPSO

Authors: S. Jalilzadeh, A. Kimiyaghalam, A. Ashouri

Abstract:

This paper presents a mathematical model and a methodology to analyze the losses in transmission expansion planning (TEP) under uncertainty in demand. The methodology is based on discrete particle swarm optimization (DPSO). DPSO is a useful and powerful stochastic evolutionary algorithm to solve the large-scale, discrete and nonlinear optimization problems like TEP. The effectiveness of the proposed idea is tested on an actual transmission network of the Azerbaijan regional electric company, Iran. The simulation results show that considering the losses even for transmission expansion planning of a network with low load growth is caused that operational costs decreases considerably and the network satisfies the requirement of delivering electric power more reliable to load centers.

Keywords: DPSO, TEP, Uncertainty

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896 Influence of Cell-free Proteins in the Nucleation of CaCO3 Crystals in Calcified Endoskeleton

Authors: M. Azizur Rahman, Tamotsu Oomori

Abstract:

Calcite aCalcite and aragonite are the two common polymorphs of CaCO3 observed as biominerals. It is universal that the sea water contents a high Mg2+ (50mM) relative to Ca2+ (10mM). In vivo crystallization, Mg2+ inhibits calcite formation. For this reason, stony corals skeleton may be formed only aragonite crystals in the biocalcification. It is special in case of soft corals of which formed only calcite crystal; however, this interesting phenomenon, still uncharacterized in the marine environment, has been explored in this study using newly purified cell-free proteins isolated from the endoskeletal sclerites of soft coral. By recording the decline of pH in vitro, the control of CaCO3 nucleation and crystal growth by the cellfree proteins was revealed. Using Atomic Force Microscope, here we find that these endoskeletal cell-free proteins significantly design the morphological shape in the molecular-scale kinetics of crystal formation and those proteins act as surfactants to promote ion attachment at calcite steps.nd aragonite are the two common polymorphs of CaCO3 observed as biominerals. It is universal that the sea water contents a high Mg2+ (50mM) relative to Ca2+ (10mM). In vivo crystallization, Mg2+ inhibits calcite formation. For this reason, stony corals skeleton may be formed only aragonite crystals in the biocalcification. It is special in case of soft corals of which formed only calcite crystal; however, this interesting phenomenon, still uncharacterized in the marine environment, has been explored in this study using newly purified cell-free proteins isolated from the endoskeletal sclerites of soft coral. By recording the decline of pH in vitro, the control of CaCO3 nucleation and crystal growth by the cell-free proteins was revealed. Using Atomic Force Microscope, here we find that these endoskeletal cell-free proteins significantly design the morphological shape in the molecular-scale kinetics of crystal formation and those proteins act as surfactants to promote ion attachment at calcite steps. KeywordsBiomineralization, Calcite, Cell-free protein, Soft coral

Keywords: Biomineralization, Calcite, Cell-free protein, Soft coral

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