Search results for: Mohammad Hossein Haddad Khodaparast

Authors: Mohammad Taghi Darvishi, Mohammad Najafi

Abstract:

By means of the extended homoclinic test approach (shortly EHTA) with the aid of a symbolic computation system such as Maple, some complexiton type solutions for the (3+1)-dimensional Jimbo-Miwa equation are presented.

Keywords: Jimbo-Miwa equation, painleve analysis, Hirota's bilinear form, computerized symbolic computation.

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Authors: Z. Haddad, C. Abid, O. Rahli, O. Margeat, W. Dachraoui, A. Mataoui

Abstract:

The present study aims to measure the volumetric mass density of NiPd-heptane nanofluids synthesized using a one step method known as thermal decomposition of metal-surfactant complexes. The particle concentration is up to 7.55g/l and the temperature range of the experiment is from 20°C to 50°C. The measured values were compared with the mixture theory and good agreement between the theoretical equation and measurement were obtained. Moreover, the available nanofluids volumetric mass density data in the literature is reviewed.

Keywords: NiPd nanoparticles, nanofluids, volumetric mass density, stability.

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Authors: Hossein Shokouhmand , Mohammad A. Esmaeili, Koohyar Vahidkhah

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This paper presents a numerical study on surface heat transfer characteristics of laminar air flows in parallel-plate dimpled channels. The two-dimensional numerical model is provided by commercial code FLUENT and the results are obtained for channels with symmetrically opposing hemi-cylindrical cavities onto both walls for Reynolds number ranging from 1000 to 2500. The influence of variations in relative depth of dimples (the ratio of cavity depth to the cavity curvature diameter), the number of them and the thermophysical properties of channel walls on heat transfer enhancement is studied. The results are evident for existence of an optimum value for the relative depth of dimples in which the largest wall heat flux and average Nusselt number can be achieved. In addition, the results of conjugation simulation indicate that the overall influence of the ratio of wall thermal conductivity to the one of the fluid on heat transfer rate is not much significant and can be ignored.

Keywords: cavity, conjugation, heat transfer, laminar air flow, Numerical, parallel-plate channel.

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Authors: Mohammad Nasri, Hossein Mirshekarpour

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We aimed to investigate how can target and optimize pulmonary delivery distribution by changing physicochemical characteristics of instilled liquid.Therefore, we created a new liquids group: a. eligible for desired distribution within lung because of assorted physicochemical characteristics b. capable of being augmented with a broad range of chemicals inertly c. no interference on respiratory function d. compatible with airway surface liquid We developed forty types of new liquid,were composed of Carboxymethylcellulose sodium,Glycerin and different types of Polysorbates.Viscosity was measured using a Programmable Rheometer and surface tension by KRUSS Tensiometer.We subsequently examined the liquids and delivery protocols by simple and branched glass capillary tube models of airways.Eventually,we explored pulmonary distribution of liquids being augmented with technetium-99m in mechanically ventilated rabbits.We used a single head large field of view gamma camera.Kinematic viscosity between 0.265Stokes and 0.289Stokes,density between 1g/cm3 and 1.5g/cm3 and surface tension between 25dyn/cm and 35dyn/cm were the most acceptable.

Keywords: Pulmonary delivery, Liquid instillation into airway, Physicochemical characteristics, Optimal distribution.

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Authors: Bahram Esmaeili, Hamid Reza Imani Far, Hossein Hosseini, Mohammad Sharifi

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The present study investigated the relationship between personality characteristics of drivers and the number and amount of fines they have in a year .This study was carried out on 120 male taxi drivers that worked at least seven hours in a day in Lamerd - a city in the south of IRAN. Subjects were chosen voluntarily among those available. Predictive variables were the NEO –five great personality factors (1. conscientiousness 2. Openness to Experience 3.Neuroticism4 .Extraversion 5.Agreeableness ) thecriterion variables were the number and amount of fines the drivers have had the last three years. the result of regression analysis showed that conscientiousness factor was able to negatively predict the number and amount of financial fines the drivers had during the last three years. The openness factor positively predicted the number of fines they had in last 3 years and the amount of financial fines during the last year. The extraversion factor both meaningfully and positively could predict only the amount of financial fines they had during the last year. Increasing age was associated with decreasing driving offenses as well as financial loss.The findings can be useful in recognizing the high-risk drivers and leading them to counseling centers .They can also be used to inform the drivers about their personality and it’s relation with their accident rate. Such criteria would be of great importance in employing drivers in different places such as companies, offices etc…

Keywords: drivers, financial fines, neo five-factor personality

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Authors: Saeid Nahi, Seyed Mohammad Hossein Nabavi

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Nowadays, power systems, energy generation by wind has been very important. Noting that the production of electrical energy by wind turbines on site to several factors (such as wind speed and profile site for the turbines, especially off the wind input speed, wind rated speed and wind output speed disconnect) is dependent. On the other hand, several different types of turbines in the market there. Therefore, selecting a turbine that its capacity could also answer the need for electric consumers the efficiency is high something is important and necessary. In this context, calculating the amount of wind power to help optimize overall network, system operation, in determining the parameters of wind power is very important. In this article, to help calculate the amount of wind power plant, connected to the national network in the region Manjil wind, selecting the best type of turbine and power delivery profile appropriate to the network using Monte Carlo method has been. In this paper, wind speed data from the wind site in Manjil, as minute and during the year has been. Necessary simulations based on Random Numbers Simulation method and repeat, using the software MATLAB and Excel has been done.

Keywords: wind turbine, efficiency, wind turbine work points, Random Numbers, reliability

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Authors: Amir. AL-Haddad, Hisham. Ettouney, Samiya. Saqer

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The main objectives of this paper are to measure pollutants concentrations in the oil refinery area in Kuwait over three periods during one year, obtain recent emission inventory for the three refineries of Kuwait, use AERMOD and the emission inventory to predict pollutants concentrations and distribution, compare model predictions against measured data, and perform numerical experiments to determine conditions at which emission rates and the resulting pollutant dispersion is below maximum allowable limits.

Keywords: Emissions, ISCST3 model, Modeling, Pollutants, Refinery

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Authors: Javad Akbari, Hassan Borzoie, Mohammad Hossein Mamduhi

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Nowadays, engineering ceramics have significant applications in different industries such as; automotive, aerospace, electrical, electronics and even martial industries due to their attractive physical and mechanical properties like very high hardness and strength at elevated temperatures, chemical stability, low friction and high wear resistance. However, these interesting properties plus low heat conductivity make their machining processes too hard, costly and time consuming. Many attempts have been made in order to make the grinding process of engineering ceramics easier and many scientists have tried to find proper techniques to economize ceramics' machining processes. This paper proposes a new diamond plunge grinding technique using ultrasonic vibration for grinding Alumina ceramic (Al2O3). For this purpose, a set of laboratory equipments have been designed and simulated using Finite Element Method (FEM) and constructed in order to be used in various measurements. The results obtained have been compared with the conventional plunge grinding process without ultrasonic vibration and indicated that the surface roughness and fracture strength improved and the grinding forces decreased.

Keywords: Engineering ceramic, Finite Element Method, Plunge grinding, Ultrasonic vibration.

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Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi

Abstract:

In this paper, the generalized (2+1)-dimensional Calogero-Bogoyavlenskii-Schiff (shortly CBS) equations are investigated. We employ the Hirota-s bilinear method to obtain the bilinear form of CBS equations. Then by the idea of extended homoclinic test approach (shortly EHTA), some exact soliton solutions including breather type solutions are presented.

Keywords: EHTA, (2+1)-dimensional CBS equations, (2+1)-dimensional breaking solution equation, Hirota's bilinear form.

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Authors: Hosein Hamisheh Bahar, Mohammad Hossein Fazel Zarandi, Akbar Esfahanipour

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In this paper, a hybrid expert system is developed by using fuzzy genetic network programming with reinforcement learning (GNP-RL). In this system, the frame-based structure of the system uses the trading rules extracted by GNP. These rules are extracted by using technical indices of the stock prices in the training time period. For developing this system, we applied fuzzy node transition and decision making in both processing and judgment nodes of GNP-RL. Consequently, using these method not only did increase the accuracy of node transition and decision making in GNP's nodes, but also extended the GNP's binary signals to ternary trading signals. In the other words, in our proposed Fuzzy GNP-RL model, a No Trade signal is added to conventional Buy or Sell signals. Finally, the obtained rules are used in a frame-based system implemented in Kappa-PC software. This developed trading system has been used to generate trading signals for ten companies listed in Tehran Stock Exchange (TSE). The simulation results in the testing time period shows that the developed system has more favorable performance in comparison with the Buy and Hold strategy.

Keywords: Fuzzy genetic network programming, hybrid expert system, technical trading signal, Tehran stock exchange.

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Authors: Mohammad Taghi Darvishi, Mohammad Najafi

Abstract:

This paper considers the (2+1)-dimensional breaking soliton equation in its bilinear form. Some exact solutions to this equation are explicitly derived by the idea of three-wave solution method with the assistance of Maple. We can see that the new idea is very simple and straightforward.

Keywords: Soliton solution, computerized symbolic computation, painleve analysis, (2+1)-dimensional breaking soliton equation, Hirota's bilinear form.

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Authors: Mohammad Mohammad

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With the exponential progress of technological development comes a strong sense that events are moving too quickly for our schools and that teachers may be losing control of them in the process. This paper examines the impact of e-learning and e-teaching in universities, from both the student and teacher perspective. In particular, it is shown that e-teachers should focus not only on the technical capacities and functions of IT materials and activities, but must attempt to more fully understand how their e-learners perceive the learning environment. From the e-learner perspective, this paper indicates that simply having IT tools available does not automatically translate into all students becoming effective learners. More evidence-based evaluative research is needed to allow e-learning and e-teaching to reach full potential.

Keywords: e-learning, e-teaching, distance learning, education

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Authors: Abolfazl Jalilvand, Mohammad Reza Feyzi, Sohrab Khanmohammad, Mohammad Bagher Bana Sharifian, Ali Sajjadi

Abstract:

Nowadays the control of stator voltage at a constant frequency is one of the traditional and low expense methods in order to control the speed of induction motors near its nominal speed. The torque of induction motor is a nonlinear function of the firing angle, phase angle and speed. In this paper the speed control of induction motor regarding various load torque and under different conditions will be investigated based on a fuzzy controller with inverse training.

Keywords: Three phase induction motor, AC converter, speedcontrol, fuzzy control.

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Authors: Mehdi Habibnia Rami, Shidvash Vakilipour, Mohammad H. Sabour, Rouzbeh Riazi, Hossein Hassannia

Abstract:

This paper deals with the steady and unsteady flow behavior on the separation bubble occurring on the rear portion of the suction side of T106A blade. The first phase was to implement the steady condition capturing the separation bubble. To accurately predict the separated region, the effects of three different turbulence models and computational grids were separately investigated. The results of Large Eddy Simulation (LES) model on the finest grid structure are acceptably in a good agreement with its relevant experimental results. The second phase is mainly to address the effects of wake entrance on bubble disappearance in unsteady situation. In the current simulations, from what was suggested in an experiment, simulating the flow unsteadiness, with concentrations on small scale disturbances instead of simulating a complete oncoming wake, is the key issue. Subsequently, the results from the current strategy to apply the effects of the wake and two other experimental work were compared to be in a good agreement. Between the two experiments, one of them deals with wake passing unsteady flow, and the other one implements experimentally the same approach as the current Computational Fluid Dynamics (CFD) simulation.

Keywords: T106A turbine cascade, shear-layer separation, steady and unsteady conditions, turbulence models, OpenFOAM.

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Authors: Mohammad Koushafar, Amir Hossein Khoshgoftarmanesh

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Due to water shortage, application of saline water for irrigation is an urgent in agriculture. In this study the effect of calcium and potassium application as additive in saline root media for reduce salinity adverse effects was investigated on tomato growth in a hydroponic system with unequal distribution of salts in the root media, which was divided in to two equal parts containing full Johnson nutrient solution and 40 mMNaCl solution, alone or in combination with KCl (6 mM), CaCl2 (4 mM), K+Ca (3+2 mM) or half-strength Johnson nutrient solution. The root splits were exchanged every 7 days. Results showed that addition of calcium, calcium-potassium and nutrition elements equivalent to half the concentration of Johnson formula to the saline-half of culture media minimized the reduction in plant growth caused by NaCl, although addition of potassium to culture media wasn’t effective. The greatest concentration of sodium was observed at the shoot of treatments which had smallest growth. According to the results of this study, in case of dynamic and non-uniform distribution of salts in the root media, by addition of additive to the saline solution, it would be possible to use of saline water with no significant growth reduction.

Keywords: Calcium, Hydroponic, Local salinity, Potassium, Saline water, Tomato.

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Authors: Karim Hamidi Machekposhti, Hossein Sedghi, Abdolrasoul Telvari, Hossein Babazadeh

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Floods have huge environmental and economic impact. Therefore, flood prediction is given a lot of attention due to its importance. This study analysed the annual maximum streamflow (discharge) (AMS or AMD) of Karkheh River in Karkheh River Basin for flood predicting using ARIMA model. For this purpose, we use the Box-Jenkins approach, which contains four-stage method model identification, parameter estimation, diagnostic checking and forecasting (predicting). The main tool used in ARIMA modelling was the SAS and SPSS software. Model identification was done by visual inspection on the ACF and PACF. SAS software computed the model parameters using the ML, CLS and ULS methods. The diagnostic checking tests, AIC criterion, RACF graph and RPACF graphs, were used for selected model verification. In this study, the best ARIMA models for Annual Maximum Discharge (AMD) time series was (4,1,1) with their AIC value of 88.87. The RACF and RPACF showed residuals’ independence. To forecast AMD for 10 future years, this model showed the ability of the model to predict floods of the river under study in the Karkheh River Basin. Model accuracy was checked by comparing the predicted and observation series by using coefficient of determination (R²).

Keywords: Time series modelling, stochastic processes, ARIMA model, Karkheh River.

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Authors: Hossein Heidari Tabrizi, Azizeh Chalak, Hossein Enayat

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The present study seeks to investigate the application of expansion strategy in Persian subtitles of English crime movies. More precisely, this study aims at classifying the different types of expansion used in subtitles as well as investigating the appropriateness or inappropriateness of the application of each type. To achieve this end, three movies; namely, The Net (1995), Contact (1997) and Mission Impossible 2 (2000), available with Persian subtitles, were selected for the study. To collect the data, the above mentioned movies were watched and those parts of the Persian subtitles in which expansion had been used were identified and extracted along with their English dialogs. Then, the extracted Persian subtitles were classified based on the reason that led to expansion in each case. Next, the appropriateness or inappropriateness of using expansion in the extracted Persian subtitles was descriptively investigated. Finally, an equivalent not containing any expansion was proposed for those cases in which the meaning could be fully transferred without this strategy. The findings of the study indicated that the reasons range from explicitation (explicitation of visual, co-textual and contextual information), mistranslation and paraphrasing to the preferences of subtitlers. Furthermore, it was found that the employment of expansion strategy was inappropriate in all cases except for those caused by explicitation of contextual information since correct and shorter equivalents which were equally capable of conveying the intended meaning could be posited for the original dialogs.

Keywords: Audiovisual translation, English crime movies, expansion strategies, Persian subtitles.

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Authors: John N. Haddad, Serge B. Provost

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Given a bivariate normal sample of correlated variables, (Xi, Yi), i = 1, . . . , n, an alternative estimator of Pearson’s correlation coefficient is obtained in terms of the ranges, |Xi − Yi|. An approximate confidence interval for ρX,Y is then derived, and a simulation study reveals that the resulting coverage probabilities are in close agreement with the set confidence levels. As well, a new approximant is provided for the density function of R, the sample correlation coefficient. A mixture involving the proposed approximate density of R, denoted by hR(r), and a density function determined from a known approximation due to R. A. Fisher is shown to accurately approximate the distribution of R. Finally, nearly exact density approximants are obtained on adjusting hR(r) by a 7th degree polynomial.

Keywords: Sample correlation coefficient, density approximation, confidence intervals.

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Authors: Mohammad Hossein Hojjati , Fardin Boustani

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Groundwater is one of the most important water resources in Fars province. Based on this study, 95 percent of the total annual water consumption in Fars is used for agriculture, whereas the percentages for domestic and industrial uses are 4 and 1 percent, respectively. Population growth, urban and industrial growth, and agricultural development in Fars have created a condition of water stress. In this province, farmers and other users are pumping groundwater faster than its natural replenishment rate, causing a continuous drop in groundwater tables and depletion of this resource. In this research variation of groundwater level , their effects and ways to help control groundwater levels in some plains of Fars were evaluated .Excessive exploitation of groundwater in Darab, Jahrom, Estahban, Arsanjan, Khir and Niriz plains of Fars caused the groundwater levels fall too fast or to unacceptable levels. The average drawdown of the water table in Arsanjan, Khir. Estahban and Niriz plain plains were 12,8, 9 and 6 meters during 16,11,11 and 13 years ago respectively. This not only reduces available water resources and well yields but also can saline water intrusion, reductions in river flow and in wetland areas , drying springs, and ground subsidence, considerable increase in pumping costs and a significant decline in crop yields as a result of the increasing salinity. Finally based on situation and condition of the aquifer some suggestions are recommended.

Keywords: Fars province , ground water overdraft , water table

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Authors: Omar M. Sallabi, Younis El-Haddad

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The Genetic Algorithm (GA) is one of the most important methods used to solve many combinatorial optimization problems. Therefore, many researchers have tried to improve the GA by using different methods and operations in order to find the optimal solution within reasonable time. This paper proposes an improved GA (IGA), where the new crossover operation, population reformulates operation, multi mutation operation, partial local optimal mutation operation, and rearrangement operation are used to solve the Traveling Salesman Problem. The proposed IGA was then compared with three GAs, which use different crossover operations and mutations. The results of this comparison show that the IGA can achieve better results for the solutions in a faster time.

Keywords: AI, Genetic algorithms, TSP.

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Authors: Liton Jude Rozario, Mohammad Reduanul Haque, Md. Ziarul Islam, Mohammad Shorif Uddin

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Face recognition is a technique to automatically identify or verify individuals. It receives great attention in identification, authentication, security and many more applications. Diverse methods had been proposed for this purpose and also a lot of comparative studies were performed. However, researchers could not reach unified conclusion. In this paper, we are reporting an extensive quantitative accuracy analysis of four most widely used face recognition algorithms: Principal Component Analysis (PCA), Independent Component Analysis (ICA), Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM) using AT&T, Sheffield and Bangladeshi people face databases under diverse situations such as illumination, alignment and pose variations.

Keywords: PCA, ICA, LDA, SVM, face recognition, noise.

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Authors: Mohammad Hasan Raouf, Azim Nowbakht, Mohammad Bagher Haddadi, Mohammad Reza Tabatabaei

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Power quality has become a very important issue recently due to the impact on electricity suppliers, equipment manufacturers and customers. Power quality is described as the variation of voltage, current and frequency in a power system. Voltage magnitude is one of the major factors that determine the quality of power. Indeed, custom power technology, the low-voltage counterpart of the more widely known flexible ac transmission system (FACTS) technology, aimed at high-voltage power transmission applications, has emerged as a credible solution to solve many problems relating to power quality problems. There are various power quality problems such as voltage sags, swells, flickers, interruptions and harmonics etc. Active Power Filter (APF) is one of the custom power devices and can mitigate harmonics, reactive power and unbalanced load currents originating from load side. In this study, an extensive review of APF studies, the advantages and disadvantages of each introduced methods are presented. The study also helps the researchers to choose the optimum control techniques and power circuit configuration for APF applications.

Keywords: Power Quality, Custom Power, Active Filter, Control Approach.

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Authors: Behrooz Rezaie, Zahra Rahmani Cherati, Mohammad Reza Jahed Motlagh, Mohammad Farrokhi

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In this paper, a fuzzy controller is designed for stabilization of the Lorenz chaotic equations. A simple Mamdani inference method is used for this purpose. This method is very simple and applicable for complex chaotic systems and it can be implemented easily. The stability of close loop system is investigated by the Lyapunov stabilization criterion. A Lyapunov function is introduced and the global stability is proven. Finally, the effectiveness of this method is illustrated by simulation results and it is shown that the performance of the system is improved.

Keywords: Chaotic system, Fuzzy control, Lorenz equation.

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Authors: Mohammad Saleh Tavazoei, Mohammad Haeri

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This paper presents comparison among methods of determination of the characteristic polynomial coefficients. First, the resultant systems from the methods are compared based on frequency criteria such as the closed loop bandwidth, gain and phase margins. Then the step responses of the resultant systems are compared on the basis of the transient behavior criteria including overshoot, rise time, settling time and error (via IAE, ITAE, ISE and ITSE integral indices). Also relative stability of the systems is compared together. Finally the best choices in regards to the above diverse criteria are presented.

Keywords: Characteristic Polynomial, Transient Response, Filters, Stability.

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Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi

Abstract:

By means of the idea of three-wave method, we obtain some analytic solutions for high nonlinear form of Benjamin-Bona- Mahony-Burgers (shortly BBMB) equations in its bilinear form.

Keywords: Benjamin-Bona-Mahony-Burgers equations, Hirota's bilinear form, three-wave method.

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Authors: Mohammad Rezaee, Mohammad Kazemeini, Ali Morad Rashidi, Moslem Fattahi

Abstract:

Selective oxidation of H2S to elemental sulfur in a fixed bed reactor over newly synthesized alumina nanocatalysts was physio-chemically investigated and results compared with a commercial Claus catalyst. Amongst these new materials, Al2O3- supported sodium oxide prepared with wet chemical technique and Al2O3 nanocatalyst prepared with spray pyrolysis method were the most active catalysts for selective oxidation of H2S to elemental sulfur. Other prepared nanocatalysts were quickly deactivated, mainly due to the interaction with H2S and conversion into sulfides.

Keywords: H2S, Claus process, Al2O3, Spray pyrolysis method, Wet chemical technique

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Authors: A. R. Nemati, M. Haddad Zarif, M. M. Fateh

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This paper presents an adaptive feedback linearization approach to derive helicopter. Ideal feedback linearization is defined for the cases when the system model is known. Adaptive feedback linearization is employed to get asymptotically exact cancellation for the inherent uncertainty in the knowledge of the given parameters of system. The control algorithm is implemented using the feedback linearization technique and adaptive method. The controller parameters are unknown where an adaptive control law aims to drive them towards their ideal values for providing perfect model matching between the reference model and the closed-loop plant model. The converged parameters of controller would then provide good estimates for the unknown plant parameters.

Keywords: Adaptive control, helicopter, feedback linearization, nonlinear control.

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Authors: Md. Sharif Imam Ibne Amir, Mohammad Masud Kamal khan, Mohammad Golam Rasul, Raj H Sharma, Fatema Akram

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Future flood can be predicted using the probable maximum flood (PMF). PMF is calculated using the historical discharge or rainfall data considering the other climatic parameters remaining stationary. However climate is changing globally and the key climatic variables are temperature, evaporation, rainfall and sea level rise are likely to change. To develop scenarios to a basin or catchment scale these important climatic variables should be considered. Nowadays scenario based on climatic variables is more suitable than PMF. Six scenarios were developed for a large Fitzroy basin and presented in this paper.

Keywords: Climate change, rainfall, potential evaporation, scenario, sea level rise (SLR), sub-catchment.

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Authors: Hossein Tavallali, Mohammad Ali Karimi, Gohar Deilamy-Rad

Abstract:

The proposed method for speciation, preconcentration and determination of Fe(II) and Fe(III) in pharmaceutical products was developed using of alumina-coated magnetite nanoparticles (Fe₃O₄/Al₂O₃ NPs) as solid phase extraction (SPE) sorbent in magnetic mixed hemimicell solid phase extraction (MMHSPE) technique followed by flame atomic absorption spectrometry analysis. The procedure is based on complexation of Fe(II) with 1, 10-phenanthroline (OP) as complexing reagent for Fe(II) that immobilized on the modified Fe₃O₄/Al₂O₃ NPs. The extraction and concentration process for pharmaceutical sample was carried out in a single step by mixing the extraction solvent, magnetic adsorbents under ultrasonic action. Then, the adsorbents were isolated from the complicated matrix easily with an external magnetic field. Fe(III) ions determined after facility reduced to Fe(II) by added a proper reduction agent to sample solutions. Compared with traditional methods, the MMHSPE method simplified the operation procedure and reduced the analysis time. Various influencing parameters on the speciation and preconcentration of trace iron, such as pH, sample volume, amount of sorbent, type and concentration of eluent, were studied. Under the optimized operating conditions, the preconcentration factor of the modified nano magnetite for Fe(II) 167 sample was obtained. The detection limits and linear range of this method for iron were 1.0 and 9.0 - 175 ng.mL⁻¹, respectively. Also the relative standard deviation for five replicate determinations of 30.00 ng.mL^-1 Fe²⁺ was 2.3%.

Keywords: Alumina-coated magnetite nanoparticles, magnetic mixed hemimicell solid-phase extraction, Fe(ΙΙ) and Fe(ΙΙΙ), pharmaceutical sample.

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Authors: Mohammad Ahmadinejad, Mohammad Reza Shakibaie, Kyvan Shams, Mohammad Khalili

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Legionella pneumophila is involved in more than 95% cases of severe atypical pneumonia. Infection is mainly by inhalation the indoor aerosols through the water-coolant systems. Because some Legionella strains may be viable but not culturable, therefore, Taq polymerase, DNA amplification and semi-nested-PCR were carried out to detect Legionella-specific 16S-rDNA sequence. For this purpose, 1.5 litter of water samples from 77 water-coolant system were collected from four different hospitals, two nursing homes and one student hostel in Kerman city of Iran, each in a brand new plastic bottle during summer season of 2006 (from April to August). The samples were filtered in the sterile condition through the Millipore Membrane Filter. DNA was extracted from membrane and used for PCR to detect Legionella spp. The PCR product was then subjected to semi-nested PCR for detection of L. pneumophila. Out of 77 water samples that were tested by PCR, 30 (39%) were positive for most species of Legionella. However, L. pneumophila was detected from 14 (18.2%) water samples by semi-nested PCR. From the above results it can be concluded that water coolant systems of different hospitals and nursing homes in Kerman city of Iran are highly contaminated with L. pneumophila spp. and pose serious concern. So, we recommend avoiding such type of coolant system in the hospitals and nursing homes.

Keywords: Legionella pneumophila, water-coolant system, semi-nested -PCR.

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