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Detection of Legionella pneumophila in Cooling Water Systems of Hospitals and Nursing Homes of Kerman City, Iran by Semi- Nested PCR
Abstract:Legionella pneumophila is involved in more than 95% cases of severe atypical pneumonia. Infection is mainly by inhalation the indoor aerosols through the water-coolant systems. Because some Legionella strains may be viable but not culturable, therefore, Taq polymerase, DNA amplification and semi-nested-PCR were carried out to detect Legionella-specific 16S-rDNA sequence. For this purpose, 1.5 litter of water samples from 77 water-coolant system were collected from four different hospitals, two nursing homes and one student hostel in Kerman city of Iran, each in a brand new plastic bottle during summer season of 2006 (from April to August). The samples were filtered in the sterile condition through the Millipore Membrane Filter. DNA was extracted from membrane and used for PCR to detect Legionella spp. The PCR product was then subjected to semi-nested PCR for detection of L. pneumophila. Out of 77 water samples that were tested by PCR, 30 (39%) were positive for most species of Legionella. However, L. pneumophila was detected from 14 (18.2%) water samples by semi-nested PCR. From the above results it can be concluded that water coolant systems of different hospitals and nursing homes in Kerman city of Iran are highly contaminated with L. pneumophila spp. and pose serious concern. So, we recommend avoiding such type of coolant system in the hospitals and nursing homes.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1071878Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1646
 R. Tello, G, Harthell, P. Costello, K. Slokes. "Legionella infected thoracic aortic graft," Comput Med Imaging Graph. 17(1), 1993, pp. 61- 77.
 P. Villari, E. Motti, C. Farullo, I, Torre. "Comparison of conventional culture and PCR methods for the detection of Legionella pneumophila in water," Lett Appl Microbiol. 27, 1998, pp. 106-110.
 F. F. Reinthaler, J. Sattler, K. Schaffler-Dullnig, B. Weinmayr, E. Marth, "Comparative Study of Procedures for Isolation and Cultivation of Legionella pneumophila from Tap Water in Hospitals" Journal of Clinical Microbiology. 31(5), 1993, pp. 1213-1216.
 W. S. Shadrach, K. Rydzewski, U. Laube, G. Holland, M. ├ûzel, A. F. Kiderlen, A. Flieger, "Balamuthia mandrillaris, Free-Living Ameba and Opportunistic Agents of Encephalitis, Is Potential Host for Legionella pneumophila Bacteria" Applied and Environmental Microbiology. 71(5), 2005, pp..2244-2249.
 A. C. England, D.W. Fraster,. "Sporadic and epidemic nasocomial legionellosis in the United States" Epidemiology features. Am J Med 70 (3), 1981, pp. 707-711.
 R. C. Mahon, G. J. Manuselis, Diagnostic Microbiology. WB Saunders Co London, 1995, pp. 430-439.
 S. Buchbinder, K. Trebesius, J. Heesemann, "Evaluation of detection of Legionella SPP. In water samples by fluorescence in situ hybridization, PCR amplification and bacterial culture" Int J Med Microbiol 292, 2002. pp. 241-145.
 H. Miyamoto, H. Yamamoto, K. Arima, J. Fujii, K. Maruta, K. Izu, T. Shiomori, S. Yoshida, "Development of a New Seminested PCR Method for Detection of Legionella Species and Its Application to Surveillance of Legionellae in Hospital Cooling Tower Water". Applied and Environmental Microbiology 63(7), 1997, 2489-2494.
 H. Yamamoto, Y. Hashimoto, T. Ezaki, "Comparison of detection methods for Legionella species in environmental water by colony isolation fluorescent antibody staining and polymerase chain reaction" Microbiol Immunol. 37, 1993 pp. 617-622.
 A. H. Movahedian, M. R. Shahmansouri, A. A. Neshat, M. Fazeli, "Identification of Legionella in the hot water supply of a General Hospital in Isfahan" J. of Research in Medical Sciences 6, 2004, pp. 40-46.
N. Wellinghausen, C. Frost, R. Marre, "Detection of Legionellae in Hospital Water Samples by Quantitative Real Time Light Cycler PCR," Applied and Environmental Microbiology. 67(9), 2001, pp. 3985-3993.
 G. F. Brooks, E. Jawetz, J. I. Melnick, E. A. Adelberg, "Legionella pneumophila In Medical Microbiology" 19th Edition. Appleton. 1991, pp. 263-266.
 M. M. Aslani, M. Pourmansour, M. Motavalian, "Isolation of Legionella pneumophila from Tehran Hospital water samples," Iranian Biochemical Journa,l 1(1), 1997, pp. 65-67.
 M. Moosavian, B. Fathollahzadeh, K. Amoli, "Isolation of Legionella pneumophila Serogroups 1, 8 & 10 (Causative Agents of Legionnairs Disease) From Water Sources in Tehran, Iran," Iranian Biomedical Journal. 2(2), 1998, pp. 83-87.
 R. Hosseini-Doust S David, "Isolation of Legionella from Hospitals Water System," Kosar Medical Journal. 3(3), 1998, 145-150.
 F. F. Reinthaler, J. Sattler, K. Schaffler-Dullnig, Comparative Study of Procedures for Isolation and Cultivation of Legionella pneumophila from Tap Water in Hospitals. Journal of Clinical Microbiology, May 1993, pp 1213 - 1216.