Search results for: Calcium phosphate cements

Authors: Mohamed S. Sasi, Adel M. Mlitan, Abdulfattah M. Alkherraz

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This research project aims to investigate difference in relative rates concerning phosphoryl transfer relevant to biological catalysis of DNA and RNA in the pH-independent reactions. Activated Models of DNA and RNA for alkyl-aryl phosphate diesters (with 4-nitrophenyl as a good leaving group) have successfully been prepared to gather kinetic parameters. Eyring plots for the pH– independent hydrolysis of 1 and 2 were established at different temperatures in the range 100–160 °C. These measurements have been used to provide a better estimate for the difference in relative rates between the reactivity of DNA and RNA cleavage. Eyring plot gave an extrapolated rate of kH2O = 1 × 10-10 s -1 for 1 (RNA model) and 2 (DNA model) at 25°C. Comparing the reactivity of RNA model and DNA model shows that the difference in relative rates in the pH-independent reactions is surprisingly very similar at 25°. This allows us to obtain chemical insights into how biological catalysts such as enzymes may have evolved to perform their current functions.

Keywords: DNA & RNA Models, Relative Rates, Reactivity.

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Authors: G. Shegani

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Coagulation is a process that sanitizes leather effluents. It aims to reduce pollutants such as Chemical Oxygen Demand (COD), chloride, sulfate, chromium, suspended solids, and other dissolved solids. The current study aimed to evaluate coagulation efficiency of tannery wastewater by analyzing the change in organic matter, odor, color, ammonium ions, nutrients, chloride, H₂S, sulfate, suspended solids, total dissolved solids, fecal pollution, and chromium hexavalent before and after treatment. Effluent samples were treated with coagulants Ca(OH)₂ and FeSO₄ ^.7H₂O. The best advantages of this treatment included the removal of: COD (81.60%); ammonia ions (98.34%); nitrate ions (92%); chromium hexavalent (75.00%); phosphate (70.00%); chloride (69.20%); and H₂S (50%). Results also indicated a high level of efficiency in the reduction of fecal pollution indicators. Unfortunately, only a modest reduction of sulfate (19.00%) and TSS (13.00%) and an increase in TDS (15.60%) was observed. 

Keywords: Coagulation, Effluent, Tannery, Treatment.

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Authors: Sandoval Villa Héctor, Estrada Velazco Evaristo, Chavarría Alamilla Luis

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Pleurotus ostreatus is a common edible mushroom with a number of properties that can help to solve the nutritional and economical problems of people in Chiapas, Mexico. The objective of this project was to produce the mushroom under a sustainable management in which only regional products were allowed as a way to promote the cultivation and consumption of Pleurotus ostreatus; 5 different substrates were tested as well as 2 sanitation methods. The obtained results showed that the highest yields were obtained using corn husk and a thermal sanitation method. Pests and diseases were not a problem during the project but they appeared more in the substrates sanitized with calcium hydroxide.

Keywords: Pleurotus ostreatus, substrates, sanitation.

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Authors: Seema Kapoor, Uma Batra

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In this study, hydroxyapatite (HA) composites are prepared on addition of 30%CaO-30%P2O5-40%Na2 O based glass to pure HA, in proportion of 2, 5, and 10 wt %. Each composition was sintered over a range of temperatures. The quantitative phase analysis was carried out using XRD and the microstructures were studied using SEM. The density, microhardness, and compressive strength have shown increase with the increasing amount of glass addition. The resulting composites have chemical compositions that are similar to the inorganic constituent of the mineral part of bone, and constitutes trace elements like Na. X-ray diffraction showed no decomposition of HA to secondary phases, however, the glass reinforced-HA composites contained a HA phase and variable amounts of tricalcium phosphate phase, depending on the amount of bioglass added. The HA-composite material exhibited higher compressive strength compared to sintered HA. The HA composite reinforced with 10 wt % bioglass showed highest bioactivity level.

Keywords: Bioactivity, Bioglass, Compressive strength, Hydroxyapatite.

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Authors: Edison Muzenda, John Kabuba, Freeman Ntuli, Mansoor Mollagee

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The aim of this study was to investigate ammonium exchange capacity of natural and activated clinoptilolite from Kwazulu-Natal Province, South Africa. X – ray fluorescence (XRF) analysis showed that the clinoptilolite contained exchangeable ions of sodium, potassium, calcium and magnesium. This analysis also confirmed that the zeolite sample had a high silicon composition compared to aluminium. Batch equilibrium studies were performed in an orbital shaker and the data fitted the Langmuir isotherm very well. The ammonium exchange capacity was found to increase with pH and temperature. Clinoptilolite functionalization with hydrochloric acid increased its ammonia uptake ability.

Keywords: Activated clinoptilolite, Ammonium exchange, Equilibrium, Functionalization, Langmuir isotherm

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Authors: Marzieh Moosavi-Nasab, Masoumeh Izadi, Sara Hosseinpour

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In this study, the possibility of using potato as a substrate for glutamic acid production by Brevibacterium linens was investigated. For preparation of fermentation medium, potato was hydrolyzed by hydrochloridric acid. The medium contained potato hydrolysate, tween 80, mineral solution, glucose, and potassium hydrogen phosphate. The initial pH of the medium was adjusted to 7-7.5. For achieving the optimum time with maximum yield, the beakers containing the medium and the inoculums were incubated in a rotary water bath flask shaker for one to five days. Thin layer choromatography was used for quantitative and qualitative assay of the glutamic acid produced. The results revealed that as fermentation time increased, pH of the fermentation medium significantly decreased (P<0.05). Furthermore, glutamic acid concentration in fermentation medium increased significantly (P<0.05). The highest amount of the glutamic acid obtained was 5.6 g/l on the forth day of fermentation.

Keywords: Brevibacterium linens, Fermentation, Glutamicacid, Thin layer choromatography

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Authors: Basharat Yousuf, Abhaya K. Srivastava

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Psyllium gum alone and in combination with sunflower oil was investigated as a possible alternative edible coating for improvement of quality and shelf life of fresh-cut papaya. Different concentrations including 0.5, 1 and 1.5 percent of psyllium gum were used for coating of fresh-cut papaya. In some samples, refined sunflower oil was used as a lipid component to increase the effectiveness of coating in terms of water barrier properties. Soya lecithin was used as an emulsifier in coatings containing oil. Pretreatment with 1% calcium chloride was given to maintain the firmness of fresh-cut papaya cubes. 1% psyllium gum coating was found to yield better results. Further, addition of oil helped to maintain the quality and acted as a barrier to water vapour, therefore, minimizing the weight loss.

Keywords: Coating, fresh-cut, gum, papaya, psylllium.

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Authors: G. Petruzzelli, F. Pedron, M. Grifoni, A. Pera, I. Rosellini, B. Pezzarossa

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The addition of lime as Ca(OH)2 to sewage sludge to destroy pathogens (Escherichia coli), was evaluated also in relation to heavy metal bioavailability. The obtained results show that the use of calcium hydroxide at the dose of 3% effectively destroyed pathogens ensuring the stability at high pH values over long period and the duration of the sewage sludge stabilization. In general, lime addition decreased the total extractability of heavy metals indicating a reduced bioavailability of these elements. This is particularly important for a safe utilization in agricultural soils to reduce the possible transfer of heavy metals to the food chain.

Keywords: Biological sludge, Ca(OH)2, copper, pathogens, sanitation, zinc.

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Authors: Alesya Ya. Maruk, Olga E. Klementyeva, Ekaterina I. Lesik, Anton A. Larenkov, Alexander B. Bruskin

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Alpfa-fetoprotein and its fragments may be an important vehicle for targeted delivery of radionuclides to the tumor. We investigated the effect of conditions on the labeling of biologically active synthetic peptide based on the (F-afp) with technetium-99m. The influence of the nature of the buffer solution, pH, concentration of reductant, concentration of the peptide and the reaction temperature on the yield of labeling was examined. As a result, the following optimal conditions for labeling of (F-afp) are found: pH 8.5 (phosphate and bicarbonate buffers) and pH from 1.7 to 7.0 (citrate buffer). The reaction proceeds with sufficient yield at room temperature for 30 min at the concentration of SnCl2 and (Fafp) (F-afp) is to be less than 10 mkg/ml and 25 mkg/ml, respectively. Investigations of the test drug accumulation in the tumor cells of human breast cancer were carried out. Results can be assumed that the in vivo study of the (F-afp) in experimental tumor lesions will show concentrations sufficient for imaging these lesions by SPECT.

Keywords: peptide, technetium-99m, tumor, SPECT.

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Authors: So-young Jung, Bu-chuhl Choe, Gi-young Shin, Jung-hun Kim, Young-zoo Chae

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A sensitive and specific method for quantitative determination of aflatoxins(B1, B2, G1,G2), deoxynivalenol, fumonisin(B1,B2), ochratoxin A, zearalenone, T-2 and HT-2 in roasted and ground grains using liquid chromatography combined with tandem mass spectrometry. A double extraction using a phosphate buffer solution followed by methanol was applied to achieve effective co extraction of 11 mycotoxins. A multitoxin immunoaffinity column for all these mycotoxins was used to clean up the extract. The LODs of mycotoxins were 0.1~6.1 μg/kg, LOQs were 0.3~18.4 μg/kg. Forty seven samples collected from Seoul (Korea) for mycotoxin contamination monitoring. The results showed that the occurrence of zearalenone and deoxynivalenol were frequent. Zearalenone was detected in all samples and deoxynivalenol was detected in 80.9 % samples in the range 0.626 ~ 29.264 μg/kg and N.D ~ 48.332 μg/kg respectively. Fumonisins and ochratoxin A were detected in 46.8% samples and 17 % samples respectively, aflatoxins and T-2/HT-2 toxins were not detected all samples.

Keywords: LC-MS/MS, mycotoxins, roasted and ground grains.

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Authors: M. Z. Othman, S. Uludag-Demirer, G. N. Demirer

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One of the main challenges for one phase anaerobic digestion processes is the high concentration of NH4+ and PO4 3- ions  in the digested sludge supernatant. This project focuses on enhancing the removal of nutrients during the anaerobic digestion process through fixing both NH4+ and PO4 3- ions in the form of struvite (magnesium ammonium phosphate, MAP, MgNH4PO4.6H2O) within the anaerobic sludge. Batch anaerobic digestion tests showed that Mg2+ concentration in the range 279 – 812 mg/L had insignificant effect on CGP but incurred a slight increase in COD removal. The reactor that had soluble Mg2+:NH4+:PO43- at a molar ratio of 1.28:1:00:1:00 achieved the best performance enhancement of 8% increase in COD removal and 32% reduction in NH4+ in the reactor supernatant. Overall, the results show that there is a potential to optimise conventional anaerobic digestion such that supernatant lean in P and N, and sludge rich in nutrients are obtained. 

Keywords: Anaerobic Digestion, Nutrients, Struvite, Waste Activated Sludge (WAS)

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Authors: Mala Nath, Nagamani Kompelli, Partha Roy, Snehasish Das

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Two new metal-based anticancer chemotherapeutic agents, [(Ph2Sn)2(HGuO)2(phen)Cl2] 1 and [(Ph3Sn)(HGuO)(phen)]- Cl.CH3OH.H2O 2, were designed, prepared and characterized by analytical and spectral (IR, ESI-Mass, 1H, 13C and 119Sn NMR) techniques. The proposed geometry of Sn(IV) in 1 and 2 is distorted octahedral and distorted trigonal-bipyramidal, respectively. Both 1 and 2 exhibit potential cytotoxicity in vitro against MCF-7, HepG-2 and DU-145 cell lines. The intrinsic binding constant (Kb) values of 1 (2.33 × 105 M-1) and 2 (2.46 × 105 M-1) evaluated from UV-Visible absorption studies suggest non-classical electrostatic mode of interaction via phosphate backbone of DNA double helix. The Stern- Volmer quenching constant (Ksv) of 1 (9.74 × 105 M-1) and 2 (2.9 × 106 M-1) determined by fluorescence studies suggests the groove binding and intercalation mode for 1 and 2, respectively. Effective cleavage of pBR322 DNA is induced by 1.Their interaction with DNA of cancer cells may account for potency.

Keywords: Anticancer agents, DNA binding studies, NMR spectroscopy, organotin.

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Authors: E. Nemes-Nagy, E. Fogarasi, M. Croitoru, A. Nyárádi, K. Komlódi, S. Pál, A. Kovács, O. Kopácsy, R. Tripon, Z. Fazakas, C. Uzun, Z. Simon-Szabó, V. Balogh-Sămărghițan, E. Ernő Nagy, M. Szabó, M. Tilinca

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Intense oxidative stress, increased glycated hemoglobin and mineral imbalance represent risk factors for complications in diabetic patients. Cardiovascular complications are most common in these patients, including nephropathy. This study was conducted in 2015 at the Procardia Laboratory in Tîrgu Mureș, Romania on 40 type 2 diabetic adults. Routine biochemical tests were performed on the Konleab 20XTi analyzer (serum glucose, total cholesterol, LDL and HDL cholesterol, triglyceride, creatinine, urea). We also measured serum uric acid, magnesium and calcium concentration by photometric procedures, potassium, sodium and chloride by ion selective electrode, and chromium by atomic absorption spectrometry in a group of patients. Glycated hemoglobin (HbA1c) dosage was made by reflectometry. Urine analysis was performed using the HandUReader equipment. The level of oxidative stress was measured by serum malondialdehyde dosage using the thiobarbituric acid reactive substances method. MDRD (Modification of Diet in Renal Disease) formula was applied for calculation of creatinine-derived glomerular filtration rate. GraphPad InStat software was used for statistical analysis of the data. The diabetic subject included in the study presented high MDA concentrations, showing intense oxidative stress. Calcium was deficient in 5% of the patients, chromium deficiency was present in 28%. The atherogenic cholesterol fraction was elevated in 13% of the patients. Positive correlation was found between creatinine and MDRD-creatinine values (p<0.0001), 68% of the patients presented increased creatinine values. The majority of the diabetic patients had good control of their diabetes, having optimal HbA1c values, 35% of them presented fasting serum glucose over 120 mg/dl and 18% had glucosuria. Intense oxidative stress and mineral deficiencies can increase the risk of cardiovascular complications in diabetic patients in spite of their good metabolic balance. More than two third of the patients present biochemical signs of nephropathy, cystatin C dosage and microalbuminuria could reveal better the kidney disorder, but glomerular filtration rate calculation formulas are also useful for evaluation of renal function.

Keywords: Cardiovascular risk, malondialdehyde, metabolic balance, minerals, type 2 diabetes.

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Authors: Sunil Kamboj, Suman Bala, Vipin Saini

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Niosomes were formulated with an aim of enhancing the oral bioavailability of losartan potassium and formulated in different molar ratios of surfactant, cholesterol and dicetyl phosphate. The formulated niosomes were found in range of 54.98 µm to 107.85 µm in size. Formulations with 1:1 ratio of surfactant and cholesterol have shown maximum entrapment efficiencies. Niosomes with sorbitan monostearate showed maximum drug release and zero order release kinetics, at the end of 24 hours. The in vivo study has shown the significant enhancement in oral bioavailability of losartan potassium in rats, after a dose of 10 mg/kg. The average relative bioavailability in relation with pure drug solution was found 2.56, indicates more than two fold increase in oral bioavailability. A significant increment in MRT reflects the release retarding ability of the vesicles. In conclusion, niosomes could be a promising delivery of losartan potassium with improved oral bioavailability and prolonged release profiles.

Keywords: Non-ionic surfactant vesicles, losartan potassium, oral bioavailability, controlled release.

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Authors: T. Utarasakul, W. Chomsopha, W. Panrod

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This study aims to initiate sustainable water management for tourist accommodations in Amphawa, Samut Songkram Province, Thailand. Wastewater generated by tourist accommodation was conducted in 10 homestays and resorts in Amphawa during August – October, 2011. The prominent parameters which are of pH, Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD), Fat Oil and Grease (FOG), Nitrate (No3-), and Phosphate (PO43-) were conducted monthly. The results revealed that some parameters were over national water quality standard (Class II). Especially, 90% of tourist accommodations have been recorded that FOG was over the standard of wastewater quality from accommodation (group I: total room of accommodation less than 200 rooms). Therefore, grease trap and natural treatment should be utilized in tourist accommodations in order to reduce the discharged of fat, oil, and grease from tourism activities. In addition, number of tourists also relate statistically with BOD and Nitrate at 0.05 level of significance.

Keywords: Sustainable Water Management, Tourist Accommodations, Environmental Protection, Samut Songkram

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Authors: J. Ramírez-Ortiz, J. Núñez Monreal

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Due to the growth of the urban area towards lands contaminated with ancient tails, in this work we evaluated if the leaching with calcium thiosulfate (CaS2O3) for the recovery of silver, gold and mercury from this soil, also dissolves arsenic, cadmium and lead; for this, we determined their quantity per each fraction of size of particle of the soil before and after the dissolution. Half of the soil samples were leached in the plant Beneficiadora de Jales del Centro, S. A. de C.V. and the rest of them remained in the laboratory. The ICP-OES technique was used to determine the amounts of arsenic, cadmium and lead, in the samples of both lots. The soil samples were collected in a neighboring area at El Lampotal, Vetagrande, Zacatecas, México, with an extension of 600 m2 at 22º52' 37.69'' N, 102º25' 11.73'' W. The amount of arsenic, cadmium and lead found in nonleached soil and for a particle size of 47 μm was 203.72±3.73, 33.63±1.31 and 3480.99±20.4 mg/kg respectively.

Keywords: Arsenic, Cadmium, Lead, Soil, Tails.

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Authors: Noor Muzamil Mohamad, Abdul Manaf Ali, Hamzah Mohd Salleh

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Phytases (myo-inositol hexakisphosphate phosphohydrolases; EC 3.1.3.8) catalyze the hydrolysis of phytic acid (myoinositol hexakisphosphate) to the mono-, di-, tri-, tetra-, and pentaphosphates of myo-inositol and inorganic phosphate. Therrmophilic bacteria isolated from water sampled from hot spring. About 120 isolates of bacteria were successfully isolated form hot spring water sample and tested for extracellular phytase producing. After 5 passages of the screening on the PSM media, 4 isolates were found stable in producing phytase enzyme. The 16s RDNA sequencing for identification of bacteria using molecular technique revealed that all isolates those positive in phytase producing are belong to Geobacillus spp. And Anoxybacillus spp. Anoxybacillus rupiensis UniSZA-7 were identified for their carbon source utilization using Phenotype Microarray Plate of Biolog and found they utilize several kind of carbon source provided.

Keywords: Phytase, Phytic Acid, Thermophilic Bacteria, PSM Media and Phytase Assay

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Authors: Nurliyani, E. Harmayani, MHNE. Soesatyo

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In Indonesia, goat milk is often consumed and believed as anti-allergy. The objective of this research was to study the effect of goat milk and their fractions (casein and whey) supplementation on total serum IgE concentrations and leukocytes count in rat sensitized with contact allergen dinitrochlorobenzene (DNCB). Female Wistar rats 6-8 weeks old were divided into four groups: 1) whey, 2) casein, 3) whole milk supplementation and 4) phosphate-buffered saline/PBS (control). The results showed that supplementation of goat milk on rats did not affects on total serum IgE concentrations and number of leukocytes. After sensitized with DNCB, the monocyte percentage in rats was higher (P<0.01) than before. In conclusion, goat milk or their fractions supplementation unable to decrease the total serum IgE concentrations and also had no effect on leukocytes count. However, 1% DNCB could increase the number of monocytes, but could not induce the IgE response.

Keywords: Dinitrochlorobenzene, Goat Milk Fractions, IgE, Leukocytes.

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Authors: Ari Istiany

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The purpose of this study was to analyze relationship between gender, BMI, and lifestyle with bone mineral density (BMD) of adolescent in urban areas . The place of this study in Jakarta State University, Indonesia. The number of samples involved as many as 200 people, consisting of 100 men and 100 women. BMD was measured using Quantitative Ultrasound Bone Densitometry. While the questionnaire used to collect data on age, gender, and lifestyle (calcium intake, smoking habits, alcohol consumption, tea, coffee, sports, and sun exposure). Mean age of men and women, respectively as much as 20.7 ± 2.18 years and 21 ± 1.61 years. Mean BMD values of men was 1.084 g/cm ² ± 0.11 while women was 0.976 g/cm ² ± 0.10. Men and women with normal BMD respectively as much as 46.7% and 16.7%. Men and women affected by osteopenia respectively as much as 50% and 80%. Men and women affected by osteoporosis respectively as much as 3.3% and 3.3%. Mean BMI of men and women, respectively as much as 21.4 ± 2.07 kg/m2 and 20.9 ± 2.06 kg/m2. Mean lifestyle score of men and women , respectively as much as 71.9 ± 5.84 and 70.1 ± 5.67 (maximum score 100). Based on Spearman and Pearson Correlation test, there were relationship significantly between gender and lifestyle with BMD.

Keywords: Adolescents, Body Mass Index (BMI), Bone Mineral Density (BMD), gender, and lifestyle.

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Authors: E. A. Kiridi, A. O. Ogunlela

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Conventional wastewater treatment plants of activated carbon, electrodialysis, ion exchange, reverse osmosis etc. are expensive to install, operate and maintain especially in developing countries; therefore, the use of aquatic macrophytes for wastewater purification is a viable alternative. On the first day of experimentation, approximately 100g of water hyacinth was introduced into the hydroponic units in four replicates. The water quality parameters measured were total suspended solids (TSS), pH and electrical conductivity (EC). Others were concentration of ammonium–nitrogen (NH₄⁺-N), nitrite-nitrogen (NO₂^--N), nitrate-nitrogen (NO₃^--N), phosphate–phosphorus (PO₄^3--P), and biomass value. At phytoremediation intervals of 7, 14, 21 and 28 days, the biomass recorded were 438.2 g, 600.7 g, 688.2 g and 725.7 g. Water hyacinth was able to reduce the pollutant concentration of all the selected parameter. The percentage reduction of pH ranged from 1.9% to 14.7%, EC from 49.8% to 97.0%, TDS from 50.4% to 97.6%, TSS from 34.0% to 78.3%, NH₄⁺-N from 38.9% to 85.2%, NO₂^--N from 0% to 84.6%, NO₃^--N from 63.2% to 98.8% and PO₄^3--P from 10% to 88.0%. Paired sample t-test shows that at 95% confidence level, it can be concluded statistically that the inequality between the pre-treatment and post-treatment values are significant. This suggests that the use of water hyacinth is valuable in the design and operation of aquaculture effluent treatment and should therefore be adopted by environmental and wastewater managers.

Keywords: Aquaculture effluent, phytoremediation, pollutant, water hyacinth.

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Authors: E. Marei, A. Hammad, S. Ismail, A. El-Gindy

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This study aims to investigate the ability of different formula of mixed bacteria as a biological treatments of wastewater after primary treatment as a bio-treatment and bio-removal and bio-adsorbent of different heavy metals in natural circumstances. The wastewater was collected from Sarpium forest site-Ismailia Governorate, Egypt. These treatments were mixture of free cells and mixture of immobilized cells of different bacteria. These different formulas of mixed bacteria were prepared under Lab. condition. The obtained data indicated that, as a result of wastewater bio-treatment, the removal rate was found to be 76.92 and 76.70% for biological oxygen demand, 79.78 and 71.07% for chemical oxygen demand, 32.45 and 36.84 % for ammonia nitrogen as well as 91.67 and 50.0% for phosphate after 24 and 28 hrs with mixed free cells and mixed immobilized cells, respectively. Moreover, the bio-removals of different heavy metals were found to reach 90.0 and 50. 0% for Cu ion, 98.0 and 98.5% for Fe ion, 97.0 and 99.3% for Mn ion, 90.0 and 90.0% Pb, 80.0% and 75.0% for Zn ion after 24 and 28 hrs with mixed free cells and mixed immobilized cells, respectively. The results indicated that 13.86 and 17.43% of removal efficiency and reduction of total dissolved solids were achieved after 24 and 28 hrs with mixed free cells and mixed immobilized cells, respectively.

Keywords: Biological desalination, bio-sorption heavy metals, free cell bacteria, immobilized bacteria, wastewater bio-treatment.

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Authors: Nice Vasconcelos Coimbra, Fábio dos Santos Gonçalves, Marisa Nascimento, Ellen Cristine Giese

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The development of chemical routes for the recovery and separation of rare earth elements (REE) is seen as a priority and strategic action by several countries demanding these elements. Among the possibilities of alternative routes, the biosorption process has been evaluated in our laboratory. In this theme, the present work attempts to assess and fit the solution equilibrium data in Langmuir, Freundlich and DKR isothermal models, based on the biosorption results of the lanthanum and samarium elements by Bacillus subtilis immobilized on calcium alginate gel. It was observed that the preference of adsorption of REE by the immobilized biomass followed the order Sm (III)> La (III). It can be concluded that among the studied isotherms models, the Langmuir model presented better mathematical results than the Freundlich and DKR models.

Keywords: Rare earth elements, biosorption, Bacillus subtilis, adsorption isotherm models.

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Authors: J. Elbl, J. K. Friedel, J. Záhora, L. Plošek, A. Kintl, J. Přichystalová, J. Hynšt, L. Dostálová, K. Zákoutská

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This work presents the first results from the long-term experiment, which is focused on the impact of intensive rainfall and long period of drought on microbial activities in soil. Fifteen lysimeters were prepared in the area of our interest. This area is a protection zone of underground source of drinking water. These lysimeters were filed with topsoil and subsoil collected in this area and divided into two groups. These groups differ in fertilization and amount of water received during the growing season. Amount of microbial biomass and leaching of mineral nitrogen and phosphates were chosen as main indicators of microbial activities in soil. Content of mineral nitrogen and phosphates was measured in soil solution, which was collected from each lysimeters. Amount of microbial biomass was determined in soil samples that were taken from the lysimeters before and after the long period of drought and intensive rainfall.

Keywords: Mineral nitrogen, Phosphates, Microbial activities, Drought, Precipitation.

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Authors: Sapna Mishra, Peeyush Kumar, Anushree Malik

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The spores of entomopathogenic fungi, Beauveria bassiana was evaluated for their compatibility with four surfactants; SDS (sodium dodyl sulphate) and CABS-65 (calcium alkyl benzene sulphonate), Tween 20 (polyethylene sorbitan monolaureate) and Tween 80 (polyoxyethylene sorbitan monoleate) at six different concentrations (0.1%, 0.5%, 1%, 2.5%, 5% and 10%). Incubated spores showed decrease in concentrations due to conversion of spores to hyphae. The maximum germination recorded in 72 h incubated spores varied with surfactant concentration at 49-68% (SDS), 39- 53% (CABS), 78-92% (Tween 80) and 80-92% (Tween 20), while the optimal surfactant concentration for spore germination was found to be 2.5-5%. The surfactant effect on spores was more pronounced with SDS and CABS-65, where significant deterioration and loss in viability of the incubated spores was observed. The effect of Tween 20 and Tween 80 were comparatively less inhibiting. The results of the study would help in surfactant selection for B. bassiana emulsion preparation.

Keywords: Beauveria bassiana, spore, surfactant, compatibility, germination.

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Authors: Abdulmajeed Bashir Mlitan, Ethan Hack

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Soil samples were collected from and around Alkomos cement factory, Alkomos town, Libya. Soil physiochemical properties were determined. In addition, olive leaves were scanned for their fungal content. This work can conclude that the results obtained for the examined physiochemical characteristics of soil in the area studied prove that cement dust from the Alkomos cement factory in Libya has had a significant impact on the soil. The affected soil properties are pH and total calcium content. These characteristics were found to be higher than those in similar soils from the same area. The increment of soil pH in the same area may be a result of precipitation of cement dust over the years. Different responses were found in each season and each site. For instance, the dominance of fungi of soil and leaves was lowest at 100 m from the factory and the evenness and diversity increased at this site compared to the control area and 250 m from the factory.

Keywords: Pollution, Soil Microbial, Alkomos, Libya.

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Authors: U. A. Asli, H. Hamid, Z.A. Zakaria, A. N. Sadikin, R. Rasit

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This study investigated the effect of a dilute acid, lime and ammonia aqueous pretreatment on the fermentable sugars conversion from empty fruit bunch (EFB) biomass. The dilute acid treatment was carried out in an autoclave, at 121ºC with 4% of sulfuric acid. In the lime pretreatment, 3 wt % of calcium hydroxide was used, whereas the third method was done by soaking EFB with 28% ammonia solution. The EFB biomass was then subjected to a two-stage-acid hydrolysis process. Subsequently, the hydrolysate was fermented by using instant baker’s yeast to produce bioethanol. The highest glucose yield was 890 mg/g of biomass, obtained from the sample which underwent lime pretreatment. The highest bioethanol yield of 6.1mg/g of glucose was achieved from acid pretreatment. This showed that the acid pretreatment gave the most fermentable sugars compared to the other two pretreatments.

Keywords: Bioethanol, biomass, empty fruit bunch (EFB), fermentable sugars.

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Authors: Burak Yigit Katanalp, Murat Tastan, Perviz Ahmedzade, Çigdem Canbay Turkyilmaz, Emrah Turkyilmaz

Abstract:

This study evaluated the mechanical and rheological performance of fly ash-based geopolymer at high temperatures. A series of laboratory tests were conducted on neat bitumen and three modified bitumen samples, which incorporated fly ash-based geopolymer at various percentages. Low-calcium fly ash was used as the alumina-silica source. The dynamic shear rheometer and rotational viscometer were employed to determine high-temperature properties, while conventional tests such as penetration and softening point were used to evaluate the physical properties of bitumen. Short-term aging resistance of the samples was assessed using the rolling thin film oven. The results show that geopolymer has a compromising effect on bitumen properties, with improved stiffness, enhanced mechanical strength, and increased thermal susceptibility of the asphalt binder.

Keywords: Bitumen, geopolymer, rutting, dynamic mechanical analysis.

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Authors: Aravindkumar B. Harwalkar, S. S. Awanti

Abstract:

Use of fly ash as a supplementary cementing material in large volumes can bring both technological and economic benefits for concrete industry. In this investigation mix proportions for high volume fly ash concrete were determined at cement replacement levels of 50%, 55%, 60% and 65% with low calcium fly ash. Flexural and compressive strengths of different mixes were measured at ages of 7, 28 and 90 days. Flexural strength of composite section prepared from pavement quality and lean high volume fly ash concrete was determined at the age of 28 days. High volume fly ash concrete mixes exhibited higher rate of strength gain and age factors than corresponding reference concrete mixes. The optimum cement replacement level for pavement quality concrete was found to be 60%. The consideration of bond between pavement quality and lean of high volume fly ash concrete will be beneficial in design of rigid pavements.

Keywords: Keywords—Composite section, Compressive strength, Flexural strength, Fly ash.

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Authors: E. A. Asabina, V. I. Pet'kov, P. A. Mayorov, A. V. Markin, N. N. Smirnova, A. M. Kovalskii, A. A. Usenko

Abstract:

The purpose of this work was to synthesize and investigate phase formation, structure and thermophysical properties of the phosphates M_0.5+xM'_xZr_2–x(PO₄)₃ (M – Cd, Sr, Pb; M' – Mg, Co, Mn). The compounds were synthesized by sol-gel method. The results showed formation of limited solid solutions of NZP/NASICON type. The crystal structures of triple phosphates of the compositions MMg_0.5Zr_1.5(PO₄)₃ were refined by the Rietveld method using XRD data. Heat capacity (8–660 K) of the phosphates Pb_0.5+xMg_xZr_2-x(PO₄)₃ (x = 0, 0.5) was measured, and reversible polymorphic transitions were found at temperatures, close to the room temperature. The results of Rietveld structure refinement showed the polymorphism caused by disordering of lead cations in the cavities of NZP/NASICON structure. Thermal expansion (298−1073 K) of the phosphates MMg_0.5Zr_1.5(PO₄)₃ was studied by XRD method, and the compounds were found to belong to middle and low-expanding materials. Thermal diffusivity (298–573 K) of the ceramic samples of phosphates slightly decreased with temperature increasing. As was demonstrated, the studied phosphates are characterized by the better thermophysical characteristics than widespread fire-resistant materials, such as zirconia and etc.

Keywords: NASICON, NZP, phosphate, structure, synthesis, thermophysical properties.

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Authors: P.Megala, T.V.Hymavathi

Abstract:

Papaya and banana bars were developed incorporating inulin (IN) and fructooligosaccharides (FOS) (Liquid and Powder form) in various proportions. The control bars were standardized using 70% fruit pulp, 30% sugar, 0.3% citric acid while the treated bars were standardized with 70% fruit pulp, 15% sugar, 15% of IN and FOS and 0.3% citric acid. Among the various proportions tested, papaya bars with 90% FOS (Powder) + 10% IN and banana bars with 90% FOS (liquid) + 10% IN were sensorially best accepted. The study revealed that addition of IN and FOS improved the sensory scores. The Physico-chemical and proximatecomposition analysis revealed slight changes in brix°, total sugars, reducing sugars, nonreducing sugars, moisture, protein, fat, vitamin C, ash, iron, zinc, calcium and crude fibre between control and treated fruit bars. Further the glycemic index of papaya bar was reduced from 65 to 54 when treated with FOS and IN.

Keywords: Banana, fructooligosaccharides, functional fruit bars, inulin, papaya

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