Different Formula of Mixed Bacteria as a Bio-Treatment for Sewage Wastewater
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 32795
Different Formula of Mixed Bacteria as a Bio-Treatment for Sewage Wastewater

Authors: E. Marei, A. Hammad, S. Ismail, A. El-Gindy


This study aims to investigate the ability of different formula of mixed bacteria as a biological treatments of wastewater after primary treatment as a bio-treatment and bio-removal and bio-adsorbent of different heavy metals in natural circumstances. The wastewater was collected from Sarpium forest site-Ismailia Governorate, Egypt. These treatments were mixture of free cells and mixture of immobilized cells of different bacteria. These different formulas of mixed bacteria were prepared under Lab. condition. The obtained data indicated that, as a result of wastewater bio-treatment, the removal rate was found to be 76.92 and 76.70% for biological oxygen demand, 79.78 and 71.07% for chemical oxygen demand, 32.45 and 36.84 % for ammonia nitrogen as well as 91.67 and 50.0% for phosphate after 24 and 28 hrs with mixed free cells and mixed immobilized cells, respectively. Moreover, the bio-removals of different heavy metals were found to reach 90.0 and 50. 0% for Cu ion, 98.0 and 98.5% for Fe ion, 97.0 and 99.3% for Mn ion, 90.0 and 90.0% Pb, 80.0% and 75.0% for Zn ion after 24 and 28 hrs with mixed free cells and mixed immobilized cells, respectively. The results indicated that 13.86 and 17.43% of removal efficiency and reduction of total dissolved solids were achieved after 24 and 28 hrs with mixed free cells and mixed immobilized cells, respectively.

Keywords: Biological desalination, bio-sorption heavy metals, free cell bacteria, immobilized bacteria, wastewater bio-treatment.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1317222

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 792


[1] S. H. I. Hanchang,"Point sources of pollution: local effects and its control –Industrial wastewater types amounts and effects," Vol. I, pp.191-203, 2009. http://www.eolss.net/Eloss-sample all chapter.aspx, (17-3-2016).
[2] http://neoakruthi.com/blog/biological treatment of wastewater.html.(12-4-2017).
[3] J. L. Wang, X. Quan, L. Han, Y. Quian, and H, Werner," Microbial degradation of quinoline by immobilized cells of Burkholderia pickettii," Water Res., vol. 36, pp. 2288-2296, 2002.
[4] H. S. U. Chen-Lung, C. F. Ouyang, and H. T. Weng, “Purification of rotating biological contactor (RBC) treated domestic wastewater for reuse in irrigation by biofilm channel," Resources conservation and Recycling, vol. 30, no. 3, pp. 165-175, 2000.
[5] A. Kumar, B. S. Bisht, V.D. Joshi, and T. Dhewa," Review on bioremediation of polluted environment: A management tool," Int. J. Environ. Sci., vol. 1, pp. 1079-1093, 2011.
[6] S. K. Rhee, G. M. Lee, and S. T. Lee," Influence of a supplementary carbon source on biodegradation of pyridine by freely suspended and immobilized Pimelobacter sp.," Appl. Microbiol. Biotechnol., vol. 44, pp. 816- 822, 1996.
[7] S. Devi, and P. Sridhar," Production of cephamycin C in repeated batch operations from immobilized Streptomyces clavuligerus," Proc. Biochem., vol. 36, pp.225-231, 2000.
[8] L. Zhou, L. Guiying, A. Taicheng, F. Jiamo, and S. Guoying," Recent patents on immobilized microorganism technology and its engineering application in wastewater treatment," Rec. Pat. Eng., vol. 2, pp. 28- 35, 2008.
[9] L. E. Bashan, and Y. Bashan," Immobilized microalgae for removing pollutants: Review of practical aspects. Bioresour," Technol., vol. 101, pp.1611-1627, 2010.
[10] M. B. Cassidy, H. Lee, and J. T. Trevors," Environmental applications of immobilized microbial cells: a review," J. Ind. Microbiol., vol. 16, pp.79-101, 1996.
[11] C. H. Hsu, Y. F. Chu, S. Argin-Soysal, T. S. Hahm, and Y. M. Lo," Effect of surface characteristics and xanthan polymers on the immobilization of Xanthomonas campestris to fibrous matrices," J. Food Sci., vol. 69, pp. 441- 448, 2004.
[12] E. Górecka, and M. Jastrzębska," Immobilization techniques and biopolymer carriers," Biotechnol. Food Sci., vol. 75, pp.65-86, 2011.
[13] S. Ismail, E. Marei, A. Hassan, and A. El-Gindy, "Biological and Bio- desalination treatments of Mixed Sewage Water," Australian Journal of Basic and Applied Sciences, vol. 11, no. 13, pp.108-114, 2017.
[14] E. M. Marei, and I. Elmaghraby," Protection of Bacillus subtilis Against Bacteriophage Attack," Current Research in Bacteriology, vol.9, no.1, pp.1-8, 2016.
[15] K. A. Gomez, and A. A. Gomez," Statistical procedures for Agricultural research with Emphasis on Rice," The international Rice Research Institute, Los-Banos, Manila, Philippines., Pp. 75-88, 1976.
[16] S. I. AbouElela, M. M. Kamel, and M. E. Fawzy," Biological treatment of saline wastewater using a salt tolerant microorganism," Des., vol. 250, no. 1, pp.1–5, 2010.
[17] M. S. Kant, and J. P. Gaur," Removal of Ni and Cu from single and binary metal solutions by free and immobilized Chlorella vulgaris," European J. Protisto., vol. 37, pp. 261-271, 2001.
[18] C. Solisio, A. Lodi, P. Torre, A. Converti, and D. M. Borghi," Copper removal by dry and re-hydrated biomass of Spirulina platensis," Technol., vol.97, pp. 1756-1760, 2006.
[19] J. I. NirmalKumar, C. Oommen, and N. K. Rita," Biosorption of heavy metals from aqueous solution by green marine macro algae from Okha Port, Gulf of Kutch India," J. Agric. Environ. Sci., vol. 6, pp. 317-323, 2009.
[20] A. Krishnan, and A. L. Neera," Waste water treatment by algae," International Journal of Innovative Research in Science Engineering and Tech., vol. 2, no. 1, pp. 286- 293, 2013.