Evaluation of Beauveria bassiana Spore Compatibility with Surfactants
The spores of entomopathogenic fungi, Beauveria bassiana was evaluated for their compatibility with four surfactants; SDS (sodium dodyl sulphate) and CABS-65 (calcium alkyl benzene sulphonate), Tween 20 (polyethylene sorbitan monolaureate) and Tween 80 (polyoxyethylene sorbitan monoleate) at six different concentrations (0.1%, 0.5%, 1%, 2.5%, 5% and 10%). Incubated spores showed decrease in concentrations due to conversion of spores to hyphae. The maximum germination recorded in 72 h incubated spores varied with surfactant concentration at 49-68% (SDS), 39- 53% (CABS), 78-92% (Tween 80) and 80-92% (Tween 20), while the optimal surfactant concentration for spore germination was found to be 2.5-5%. The surfactant effect on spores was more pronounced with SDS and CABS-65, where significant deterioration and loss in viability of the incubated spores was observed. The effect of Tween 20 and Tween 80 were comparatively less inhibiting. The results of the study would help in surfactant selection for B. bassiana emulsion preparation.
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 Mishra S, Kumar P, Malik A, Satya S (2011) Adulticidal and larvicidal activity of Beauveria bassiana and Metarhizium anisopliae against housefly, Musca domestica (Diptera: Muscidae), in laboratory and simulated field bioassays. Parasitol Res 108: 1483-1492
 Mishra S, Malik A (2012a) Comparative evaluation of five Beauveria isolates for housefly (Musca domestica L.) control and growth optimization of selected strain. Parasitol Res DOI 10.1007/s00436-012- 3039-y
 Lecuona RE, Turica M, Tarocco F, Crespo DC (2005) Microbial control of Musca domestica (Diptera: Muscidae) with selected strains of Beauveria bassiana. J Med Entomol 42:332-336
 Pirali-Kheirabadi K, Haddadzadeh H, Razzaghi-Abyaneh M, Bokaie S, Zare R, Ghazavi M, Shams-Ghahfarokhi M (2007) Biological control of Rhipicephalus (Boophilus) annulatus by different strains of Metarhizium anisopliae, Beauveria bassiana and Lecanicillium psalliotae fungi. Parasitol Res 100:1297-1302
 Mishra S, Malik A (2012b) Nutritional optimization of a native Beauveria bassiana isolate (HQ917687) pathogenic to housefly, Musca domestica L. J Parasit dis Accepted
 Meikle WG, Mercadier G, Holst N, Girod V (2008) Impact of two treatments of a formulation of Beauveria bassiana (Deuteromycota: Hyphomycetes) conidia on Varroa mites (Acari: Varroidae) and on honeybee (Hymenoptera: Apidae) colony health. Exp Appl Acarol 46:105-17
 Luz C, Batagin I (2005) Potential of oil-based formulations of Beauveria bassiana to control Triatoma infestans. Mycopathol 160:51-62
 Woertz JR, Kinney KA (2004). Influence of sodium dodecyl sulfate and Tween 20 on fungal growth and toluene degradation in a vapor-phase bioreactor. J. Environ. Eng. 130: 292-299.
 Miehle BR, Lukezic FL (1972). Studies on conidial germination and appressorium formation by Colletotrichum trifolii Bain & Essary. Can. J. Microbiol 18: 1263-1269.
 Jin X, Streett DA, Dunlap CA, Lyn ME (2008) Application of hydrophilic-lipophilic balance (HLB) number to optimize a compatible non-ionic surfactant for dried aerial conidia of Beauveria bassiana. Biol Control 46:226-233
 StatPlus (2007) Professional build 220.127.116.11. AnalystSoft.
 Tanuja K, Hemalatha K, Karuna R, Sashidhar Rao B (2010) Effect of various surfactants (cationic, anionic and non-ionic) on the growth of Aspergillus parasiticus (NRRL 2999) in relation to aflatoxin production. Mycotox Res 26:155-170
 Kollmann A, Brault A, Touton I, Dubroca J, Chaplain V, Mougin C (2003) Impact of nonylphenol surfactants on fungi following the application of sewage sludge on agricultural soils. J Environ Qual 32(4): 1269-1276.
 Thomson CA, Delaquis PJ, Mazza G (1999) Detection and measurement of microbial lipase activity: a review. Crit Rev Food Sci Nutr 39:165-87
 Silva WOB, Mitidieri S, Schrank A, Vainstein MH (2005) Production and extraction of an extracellular lipase from the entomopathogenic fungus Metarhizium anisopliae. Process Biochem 40: 321-326
 Prasad R (1993) Role of adjuvants in modifying the efficacy of a bioherbicide on forest species: Compatibility studies under laboratory conditions. Pesticide Sci 38:273-275
 Polar P, Kairo MTK, Moore D, Pegram R, John SA (2005) Comparison of water, oils and emulsifiable adjuvant oils as formulating agents for Metarhizium anisopliae for use in control of Boophilus microplus. Mycopathol 160:151-157
 Long K, Knapp JS (1991) The effect of Junlon PWII0 and Tween 80 on the production of cellulolytic enzymes by Coprinus cinereus. Mycol. Res 95 (9): 1077-1081.