Search results for: Surface Finish.
616 Adsorption of Bovine Serum Albumin on CeO2
Authors: Roman Marsalek
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Preparation of nanoparticles of cerium oxide and adsorption of bovine serum albumin on them were studied. Particle size distribution and influence of pH on zeta potential of prepared CeO2 were determined. Average size of prepared cerium oxide nanoparticles was 9 nm. The simultaneous measurements of the bovine serum albumin adsorption and zeta potential determination of the (adsorption) suspensions were carried out. The adsorption isotherms were found to be of typical Langmuir type; values of the bovine serum albumin adsorption capacities were calculated. Increasing of pH led to decrease of zeta potential and decrease of adsorption capacity of cerium oxide nanoparticles. The maximum adsorption capacity was found for strongly acid suspension (am = 118 mg/g). The samples of nanoceria with positive zeta potential adsorbed more bovine serum albumin on the other hand, the samples with negative zeta potential showed little or no protein adsorption. Surface charge or better say zeta potential of CeO2 nanoparticles plays the key role in adsorption of proteins on such type of materials.
Keywords: Adsorption, BSA, cerium oxide nanoparticles, zeta potential.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3047615 The Effect of Gross Vehicle Weight on the Stability of Heavy Vehicle during Cornering
Authors: Nurzaki Ikhsan, Ahmad Saifizul Abdullah, Rahizar Ramli
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One of the functions of the commercial heavy vehicle is to safely and efficiently transport goods and people. Due to its size and carrying capacity, it is important to study the vehicle dynamic stability during cornering. Study has shown that there are a number of overloaded heavy vehicles or permissible Gross Vehicle Weight (GVW) violations recorded at selected areas in Malaysia assigned by its type and category. Thus, the objective of this study is to investigate the correlation and effect of the GVW on heavy vehicle stability during cornering event using simulation. Various selected heavy vehicle types and category are simulated using IPG/Truck Maker® with different GVW and road condition (coefficient of friction of road surface), while the speed, driver characteristic, center of gravity of load and road geometry are constant. Based on the analysis, the relationship between GVW and lateral acceleration were established. As expected, on the same value of coefficient of friction, the maximum lateral acceleration would be increased as the GVW increases.Keywords: Heavy Vehicle, Road Safety, Vehicle Stability, Lateral Acceleration, Gross Vehicle Weight.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3090614 On the Numerical Approach for Simulating Thermal Hydraulics under Seismic Condition
Authors: Tadashi Watanabe
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The two-phase flow field and the motion of the free surface in an oscillating channel are simulated numerically to assess the methodology for simulating nuclear reacotr thermal hydraulics under seismic conditions. Two numerical methods are compared: one is to model the oscillating channel directly using the moving grid of the Arbitrary Lagrangian-Eulerian method, and the other is to simulate the effect of channel motion using the oscillating acceleration acting on the fluid in the stationary channel. The two-phase flow field in the oscillating channel is simulated using the level set method in both cases. The calculated results using the oscillating acceleration are found to coinside with those using the moving grid, and the theoretical back ground and the limitation of oscillating acceleration are discussed. It is shown that the change in the interfacial area between liquid and gas phases under seismic conditions is important for nuclear reactor thermal hydraulics.Keywords: Two-phase flow, simulation, seismic condition, moving grid, oscillating acceleration, interfacial area
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1378613 Crack Opening Investigation in Fiberconcrete
Authors: Arturs Macanovskis, Vitalijs Lusis, Andrejs Krasnikovs
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This work had three stages. In the first stage was examined pull-out process for steel fiber was embedded into a concrete by one end and was pulled out of concrete under the angle to pulling out force direction. Angle was varied. On the obtained forcedisplacement diagrams were observed jumps. For such mechanical behavior explanation, fiber channel in concrete surface microscopical experimental investigation, using microscope KEYENCE VHX2000, was performed. At the second stage were obtained diagrams for load- crack opening displacement for breaking homogeneously reinforced and layered fiberconcrete prisms (with dimensions 10x10x40cm) subjected to 4-point bending. After testing was analyzed main crack. At the third stage elaborated prediction model for the fiberconcrete beam, failure under bending, using the following data: a) diagrams for fibers pulling out at different angles; b) experimental data about steel-straight fibers locations in the main crack. Experimental and theoretical (modeling) data were compared.
Keywords: Fiberconcrete, pull-out, fiber channel, layered fiberconcrete.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1855612 Numerical Analysis of Roughness Effect on Mini and Microchannels: Hydrodynamics and Heat Transfer
Authors: El-Ghalia Filali, Cherif Gadouche, Mohamed Tahar
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A three-dimensional numerical simulation of flow through mini and microchannels with designed roughness is conducted here. The effect of the roughness height (surface roughness), geometry, Reynolds number on the friction factor, and Nusselt number is investigated. The study is carried out by employing CFD software, CFX. Our work focuses on a water flow inside a circular mini-channel of 1 mm and microchannels of 500 and 100 m in diameter. The speed entry varies from 0.1 m/s to 20 m/s. The general trend can be observed that bigger sizes of roughness element lead to higher flow resistance. It is found that the friction factor increases in a nonlinear fashion with the increase in obstruction height. Particularly, the effect of roughness can no longer be ignored at relative roughness height higher than 3%. A significant increase in Poiseuille number is detected for all configurations considered. The same observation can be done for Nusselt number. The transition zone between laminar and turbulent flow depends on the channel diameter.Keywords: Heat transfer, hydrodynamics, micro-channel, roughness.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1768611 Study of the Oxidation Resistance of Coated AISI 441 Ferritic Stainless Steel for SOFCs
Authors: M. B. Limooei, Hadi Ebrahimifar, Sh. Hosseini
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Protective coatings that resist oxide scale growth and decrease chromium evaporation are necessary to make stainless steel interconnect materials for long-term durable operation of solid oxide fuel cells (SOFCs). In this study a layer of cobalt was electroplated on the surface of AISI 441 ferritic stainless steel which is used in solid oxide fuel cells for interconnect applications. The oxidation behavior of coated substrates was studied as a function of time at operating conditions of SOFCs. Cyclic oxidation has been also tested at 800ºC for 100 cycles. Cobalt coating during isothermal oxidation caused to the oxide growth resistance by limiting the outward diffusion of Cr cation and the inward diffusion of oxygen anion. Results of cyclic oxidation exhibited that coated substrates demonstrate an excellent resistance against the spallation and cracking.
Keywords: Oxidation resistance, full cell, Cobalt coating, ferritic stainless steel.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2032610 Improved Technique of Non-viral Gene Delivery into Cancer Cells
Authors: D. Vainauska, S. Kozireva, A. Karpovs, M. Chistyakovs, M. Baryshev
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Liposomal magnetofection is a simple, highly efficient technology for cell transfection, demonstrating better outcome than a number of other common gene delivery methods. However, aggregate complexes distribution over the cell surface is non-uniform due to the gradient of the permanent magnetic field. The aim of this study was to estimate the efficiency of liposomal magnetofection for prostate carcinoma PC3 cell line using newly designed device, “DynaFECTOR", ensuring magnetofection in a dynamic gradient magnetic field. Liposomal magnetofection in a dynamic gradient magnetic field demonstrated the highest transfection efficiency for PC3 cells – it increased for 21% in comparison with liposomal magnetofection and for 42% in comparison with lipofection alone. The optimal incubation time under dynamic magnetic field for PC3 cell line was 5 minutes and the optimal rotation frequency of magnets – 5 rpm. The new approach also revealed lower cytotoxic effect to cells than liposomal magnetofection.Keywords: Dynamic gradient magnetic field, gene delivery, liposomal magnetofection, prostate cancer cell line
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1664609 An Evaluation of the Oxide Layers in Machining Swarfs to Improve Recycling
Authors: J. Uka, B. McKay, T. Minton, O. Adole, R. Lewis, S. J. Glanvill, L. Anguilano
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Effective heat treatment conditions to obtain maximum aluminium swarf recycling are investigated in this work. Aluminium swarf briquettes underwent treatments at different temperatures and cooling times to investigate the improvements obtained in the recovery of aluminium metal. The main issue for the recovery of the metal from swarfs is to overcome the constraints due to the oxide layers present in high concentration in the swarfs since they have a high surface area. Briquettes supplied by Renishaw were heat treated at 650, 700, 750, 800 and 850 ℃ for 1-hour and then cooled at 2.3, 3.5 and 5 ℃/min. The resulting material was analysed using SEM EDX to observe the oxygen diffusion and aluminium coalescence at the boundary between adjacent swarfs. Preliminary results show that, swarf needs to be heat treated at a temperature of 850 ℃ and cooled down slowly at 2.3 ℃/min to have thin and discontinuous alumina layers between the adjacent swarf and consequently allowing aluminium coalescence. This has the potential to save energy and provide maximum financial profit in preparation of swarf briquettes for recycling.
Keywords: Aluminium, swarf, oxide layers, recycle, reuse.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 573608 A Preliminary Study of Drug Perfusion Enhancement by Microstreaming Induced by an Oscillating Microbubble
Authors: Jin Sun Oh, Kyung Ho Lee, S ang Gug Chung, Kyehan Rhee
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Microbubbbles incorporating ultrasound have been used to increase the efficacy of targeted drug delivery, because microstreaming induced by cavitating bubbles affects the drug perfusion into the target cells and tissues. In order to clarify the physical effects of microstreaming on drug perfusion into tissues, a preliminary experimental study of perfusion enhancement by a stably oscillating microbubble was performed. Microstreaming was induced by an oscillating bubble at 15 kHz, and perfusion of dye into an agar phantom was optically measured by histology on agar phantom. Surface color intensity and the penetration length of dye in the agar phantom were increased more than 70% and 30%, respectively, due to the microstreaming induced by an oscillating bubble. The mass of dye perfused into a tissue phantom for 30 s was increased about 80% in the phantom with an oscillating bubble. This preliminary experiment shows the physical effects of steady streaming by an oscillating bubble can enhance the drug perfusion into the tissues while minimizing the biological effects.
Keywords: Bubble, Mass Transfer, Microstreaming, Drug Delivery, Acoustic Wave.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1885607 Estimating the Effect of Fluid in Pressing Process
Authors: A. Movaghar, R. A. Mahdavinejad
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To analyze the effect of various parameters of fluid on the material properties such as surface and depth defects and/or cracks, it is possible to determine the affection of pressure field on these specifications. Stress tensor analysis is also able to determine the points in which the probability of defection creation is more. Besides, from pressure field, it is possible to analyze the affection of various fluid specifications such as viscosity and density on defect created in the material. In this research, the concerned boundary conditions are analyzed first. Then the solution network and stencil used are mentioned. With the determination of relevant equation on the fluid flow between notch and matrix and their discretion according to the governed boundary conditions, these equations can be solved. Finally, with the variation creations on fluid parameters such as density and viscosity, the affection of these variations can be determined on pressure field. In this direction, the flowchart and solution algorithm with their results as vortex and current function contours for two conditions with most applications in pressing process are introduced and discussed.
Keywords: Pressing, notch, matrix, flow function, vortex.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 704606 Failure Analysis of a Medium Duty Vehicle Leaf Spring
Authors: Gül Çevik
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This paper summarizes the work conducted to assess the root cause of the failure of a medium commercial vehicle leaf spring failed in service. Macro- and micro-fractographic analyses by scanning electron microscope as well as material verification tests were conducted in order to understand the failure mechanisms and root cause of the failure. Findings from the fractographic analyses indicated that failure mechanism is fatigue. Crack initiation was identified to have occurred from a point on the top surface near to the front face and to the left side. Two other crack initiation points were also observed, however, these cracks did not propagate. The propagation mode of the fatigue crack revealed that the cyclic loads resulting in crack initiation and propagation were unidirectional bending. Fractographic analyses have also showed that the root cause of the fatigue crack initiation and propagation was loading the part above design stress. Material properties of the part were also verified by chemical composition analysis, microstructural analysis by optical microscopy and hardness tests.
Keywords: Leaf spring, failure analysis, fatigue, fractography.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 743605 Numerical Analysis of Dynamic Responses of the Plate Subjected to Impulsive Loads
Authors: Behzad Mohammadzadeh, Huyk Chun Noh
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Plate is one of the popular structural elements used in a wide range of industries and structures. They may be subjected to blast loads during explosion events, missile attacks or aircraft attacks. This study is to investigate dynamic responses of the rectangular plate subjected to explosive loads. The effects of material properties and plate thickness on responses of the plate are to be investigated. The compressive pressure is applied to the surface of the plate. Different amounts of thickness in the range from 1mm to 30mm are considered for the plate to evaluate the changes in responses of the plate with respect to plate thickness. Two different properties are considered for the steel. First, the analysis is performed by considering only the elastic-plastic properties for the steel plate. Later on damping is considered to investigate its effects on the responses of the plate. To do analysis, numerical method using a finite element based package ABAQUS is applied. Finally, dynamic responses and graphs showing the relation between maximum displacement of the plate and aim parameters are provided.
Keywords: Impulsive loaded plates, dynamic analysis, abaqus, material nonlinearity.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1821604 Experimental Investigation and Optimization of Nanoparticle Mass Concentration and Heat Input of Loop Heat Pipe
Authors: P. Gunnasegaran, M. Z. Abdullah, M. Z. Yusoff, Nur Irmawati
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This study presents experimental and optimization of nanoparticle mass concentration and heat input based on the total thermal resistance (Rth) of loop heat pipe (LHP), employed for PCCPU cooling. In this study, silica nanoparticles (SiO2) in water with particle mass concentration ranged from 0% (pure water) to 1% is considered as the working fluid within the LHP. The experimental design and optimization is accomplished by the design of experimental tool, Response Surface Methodology (RSM). The results show that the nanoparticle mass concentration and the heat input have significant effect on the Rth of LHP. For a given heat input, the Rth is found to decrease with the increase of the nanoparticle mass concentration up to 0.5% and increased thereafter. It is also found that the Rth is decreased when the heat input is increased from 20W to 60W. The results are optimized with the objective of minimizing the Rth, using Design-Expert software, and the optimized nanoparticle mass concentration and heat input are 0.48% and 59.97W, respectively, the minimum thermal resistance being 2.66 (ºC/W).Keywords: Loop heat pipe, nanofluid, optimization, thermal resistance.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1823603 Free Vibration Analysis of Smart FGM Plates
Authors: F.Ebrahimi, A.Rastgo
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Analytical investigation of the free vibration behavior of circular functionally graded (FG) plates integrated with two uniformly distributed actuator layers made of piezoelectric (PZT4) material on the top and bottom surfaces of the circular FG plate based on the classical plate theory (CPT) is presented in this paper. The material properties of the functionally graded substrate plate are assumed to be graded in the thickness direction according to the power-law distribution in terms of the volume fractions of the constituents and the distribution of electric potential field along the thickness direction of piezoelectric layers is simulated by a quadratic function. The differential equations of motion are solved analytically for clamped edge boundary condition of the plate. The detailed mathematical derivations are presented and Numerical investigations are performed for FG plates with two surface-bonded piezoelectric layers. Emphasis is placed on investigating the effect of varying the gradient index of FG plate on the free vibration characteristics of the structure. The results are verified by those obtained from threedimensional finite element analyses.Keywords: Circular plate, CPT, Functionally graded, Piezoelectric.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2299602 Analysis of Rail Ends under Wheel Contact Loading
Authors: Nannan Zong, Manicka Dhanasekar
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The effect of the discontinuity of the rail ends and the presence of lower modulus insulation material at the gap to the variations of stresses in the insulated rail joint (IRJ) is presented. A three-dimensional wheel – rail contact model in the finite element framework is used for the analysis. It is shown that the maximum stress occurs in the subsurface of the railhead when the wheel contact occurs far away from the rail end and migrates to the railhead surface as the wheel approaches the rail end; under this condition, the interface between the rail ends and the insulation material has suffered significantly increased levels of stress concentration. The ratio of the elastic modulus of the railhead and insulation material is found to alter the levels of stress concentration. Numerical result indicates that a higher elastic modulus insulating material can reduce the stress concentration in the railhead but will generate higher stresses in the insulation material, leading to earlier failure of the insulation materialKeywords: Rail end, material interface, wheel-rail contact, stress, finite element method
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2427601 Formation of Chemical Compound Layer at the Interface of Initial Substances A and B with Dominance of Diffusion of the A Atoms
Authors: Pavlo Selyshchev, Samuel Akintunde
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A theoretical approach to consider formation of chemical compound layer at the interface between initial substances A and B due to the interfacial interaction and diffusion is developed. It is considered situation when speed of interfacial interaction is large enough and diffusion of A-atoms through AB-layer is much more then diffusion of B-atoms. Atoms from A-layer diffuse toward B-atoms and form AB-atoms on the surface of B-layer. B-atoms are assumed to be immobile. The growth kinetics of the AB-layer is described by two differential equations with non-linear coupling, producing a good fit to the experimental data. It is shown that growth of the thickness of the AB-layer determines by dependence of chemical reaction rate on reactants concentration. In special case the thickness of the AB-layer can grow linearly or parabolically depending on that which of processes (interaction or the diffusion) controls the growth. The thickness of AB-layer as function of time is obtained. The moment of time (transition point) at which the linear growth are changed by parabolic is found.
Keywords: Phase formation, Binary systems, Interfacial Reaction, Diffusion, Compound layers, Growth kinetics.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1760600 Corrosion Protection of Structural Steel by Surfactant Containing Reagents
Authors: D. Erdenechimeg, T. Bujinlkham, N. Erdenepurev
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The anti-corrosion performance of fatty acid coated mild steel samples is studied. Samples of structural steel coated with collector reagents deposited from surfactant in ethanol solution and overcoated with an epoxy barrier paint. A quantitative corrosion rate was determined by linear polarization resistance method using biopotentiostat/galvanostat 400. Coating morphology was determined by scanning electronic microscopy. A test for hydrophobic surface of steel by surfactant was done. From the samples, the main component or high content iron was determined by chemical method and other metal contents were determined by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) method. Prior to measuring the corrosion rate, mechanical and chemical treatments were performed to prepare the test specimens. Overcoating the metal samples with epoxy barrier paint after exposing them with surfactant the corrosion rate can be inhibited by 34-35 µm/year.
Keywords: Corrosion, linear polarization resistance, coating, surfactant.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 646599 New Drug Delivery System for Cancer Therapy
Authors: Emma R. Arakelova, Stepan G. Grigoryan, Ashot M. Khachatryan, Karapet E. Avjyan, Lilia M. Savchenko, Flora G. Arsenyan
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The paper presents a new drugs delivery system, based on the thin film technology. As a model antitumor drug, highly toxic doxorubicin is chosen. The system is based on the technology of obtaining zinc oxide composite of doxorubicin by deposition of nanosize ZnO films on the surface of doxorubicin coating on glass substrate using DC magnetron sputtering of zinc targets in Ar:O2 medium at room temperature. For doxorubicin zinc oxide compositions in the form of coatings and gels with 180-200nm thick ZnO films, higher (by a factor 2) in vivo (ascitic Ehrlich's carcinoma) antitumor activity is observed at low doses of doxorubicin in comparison with that of the initial preparation at therapeutic doses. The vector character of the doxorubicin zinc oxide composite transport to tumor tissues ensures the increase in antitumor activity as well as decrease of toxicity in comparison with the initial drug.
Keywords: Antitumor activity, doxorubicin, DC magnetron sputtering, zinc oxide.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3614598 Separation of Chlorinated Plastics and Immobilization of Heavy Metals in Hazardous Automotive Shredder Residue
Authors: Srinivasa Reddy Mallampati, Chi-Hyeon Lee, Nguyen Thi Thanh Truc, Byeong-Kyu Lee
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In the present study, feasibility of the selective surface hydrophilization of polyvinyl chloride (PVC) by microwave treatment was evaluated to facilitate the separation from automotive shredder residue (ASR), by the froth flotation. The combination of 60 sec microwave treatment with PAC, a sharp and significant decrease about 16.5° contact angle of PVC was observed in ASR plastic compared with other plastics. The microwave treatment with the addition of PAC resulted in a synergetic effect for the froth flotation, which may be a result of the 90% selective separation of PVC from ASR plastics, with 82% purity. While, simple mixing with a nanometallic Ca/CaO/PO4 dispersion mixture immobilized 95-100% of heavy metals in ASR soil/residues. The quantity of heavy metals leached from thermal residues after treatment by nanometallic Ca/CaO/PO4 was lower than the Korean standard regulatory limit for hazardous waste landfills. Microwave treatment can be a simple and effective method for PVC separation from ASR plastics.Keywords: Automotive shredder residue, microwave treatment, chlorinated plastics, separation, heavy metals, Immobilization, separation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2074597 Removal of Chlorinated Resin and Fatty Acids from Paper Mill wastewater through Constructed Wetland
Authors: Ashutosh Kumar Choudhary, Satish Kumar, Chhaya Sharma
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This study evaluates the performance of horizontal subsurface flow constructed wetland (HSSF-CW) for the removal of chlorinated resin and fatty acids (RFAs) from pulp and paper mill wastewater. The dimensions of the treatment system were 3.5 m x 1.5 m x 0.28 m with surface area of 5.25 m2, filled with fine sand and gravel. The cell was planted with an ornamental plant species Canna indica. The removal efficiency of chlorinated RFAs was in the range of 92-96% at the hydraulic retention time (HRT) of 5.9 days. Plant biomass and soil (sand and gravel) were analyzed for chlorinated RFAs content. No chlorinated RFAs were detected in plant biomass but detected in soil samples. Mass balance studies of chlorinated RFAs in HSSF-CW were also carried out.Keywords: Canna indica, Chlorinated resin & fatty acids, Constructed wetland, Pulp and paper mill wastewater.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2169596 Hydrogel Based on Cellulose Acetate Used as Scaffold for Cell Growth
Authors: A. Maria G. Melero, A. M. Senna, J. A. Domingues, M. A. Hausen, E. Aparecida R. Duek, V. R. Botaro
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A hydrogel from cellulose acetate cross linked with ethylenediaminetetraacetic dianhydride (HAC-EDTA) was synthesized by our research group, and submitted to characterization and biological tests. Cytocompatibility analysis was performed by confocal microscopy using human adipocyte derived stem cells (ASCs). The FTIR analysis showed characteristic bands of cellulose acetate and hydroxyl groups and the tensile tests evidence that HAC-EDTA present a Young’s modulus of 643.7 MPa. The confocal analysis revealed that there was cell growth at the surface of HAC-EDTA. After one day of culture the cells presented spherical morphology, which may be caused by stress of the sequestration of Ca2+ and Mg2+ ions at the cell medium by HAC-EDTA, as demonstrated by ICP-MS. However, after seven days and 14 days of culture, the cells present fibroblastoid morphology, phenotype expected by this cellular type. The results give efforts to indicate this new material as a potential biomaterial for tissue engineering, in the future in vivo approach.
Keywords: Cellulose acetate, hydrogel, biomaterial, cellular growth.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1161595 The Sequestration of Heavy Metals Contaminating the Wonderfonteinspruit Catchment Area using Natural Zeolite
Authors: P.P. Diale, S.S.L. Mkhize, E. Muzenda, J. Zimba
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For more than 120 years, gold mining formed the backbone the South Africa-s economy. The consequence of mine closure was observed in large-scale land degradation and widespread pollution of surface water and groundwater. This paper investigates the feasibility of using natural zeolite in removing heavy metals contaminating the Wonderfonteinspruit Catchment Area (WCA), a water stream with high levels of heavy metals and radionuclide pollution. Batch experiments were conducted to study the adsorption behavior of natural zeolite with respect to Fe2+, Mn2+, Ni2+, and Zn2+. The data was analysed using the Langmuir and Freudlich isotherms. Langmuir was found to correlate the adsorption of Fe2+, Mn2+, Ni2+, and Zn2+ better, with the adsorption capacity of 11.9 mg/g, 1.2 mg/g, 1.3 mg/g, and 14.7 mg/g, respectively. Two kinetic models namely, pseudo-first order and pseudo second order were also tested to fit the data. Pseudo-second order equation was found to be the best fit for the adsorption of heavy metals by natural zeolite. Zeolite functionalization with humic acid increased its uptake ability.Keywords: gold-mining, natural zeolites, water pollution, WestRand.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2525594 New Device for Enhancement of Liposomal Magnetofection Efficiency of Cancer Cells
Authors: M. Baryshev, D.Vainauska, S. Kozireva, A.Karpovs
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Liposomal magnetofection is the most powerful nonviral method for the nucleic acid delivery into the cultured cancer cells and widely used for in vitro applications. Use of the static magnetic field condition may result in non-uniform distribution of aggregate complexes on the surface of cultured cells. To prevent this, we developed the new device which allows to concentrate aggregate complexes under dynamic magnetic field, assisting more contact of these complexes with cellular membrane and, possibly, stimulating endocytosis. Newly developed device for magnetofection under dynamic gradient magnetic field, “DynaFECTOR", was used to compare transfection efficiency of human liver hepatocellular carcinoma cell line HepG2 with that obtained by lipofection and magnetofection. The effect of two parameters on transfection efficiency, incubation time under dynamic magnetic field and rotation frequency of magnet, was estimated. Liposomal magnetofection under dynamic gradient magnetic field showed the highest transfection efficiency for HepG2 cells.
Keywords: Dynamic magnetic field, Lipofection, Magnetofection
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1762593 Experiment Study on the Plasma Parameters Measurement in Backflow Region of Ion Thruster
Authors: Tian Kai, Yang Sheng-sheng, Li De-tian, Miao Yu-jun, Xue Yu-xiong Wang Yi, Yan Ze-dong, Ma Ya-li, ZHuang Jian-hong
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The charge-exchange xenon (CEX) ion generated by ion thruster can backflow to the surface of spacecraft and threaten to the safety of spacecraft operation. In order to evaluate the effects of the induced plasma environment in backflow regions on the spacecraft, we designed a spherical single Langmuir probe of 5.8cm in diameter for measuring low-density plasma parameters in backflow region of ion thruster. In practice, the tests are performed in a two-dimensional array (40cm×60cm) composed of 20 sites. The experiment results illustrate that the electron temperature ranges from 3.71eV to 3.96eV, with the mean value of 3.82eV and the standard deviation of 0.064eV. The electron density ranges from 8.30×1012/m3 to 1.66×1013/m3, with the mean value of 1.30×1013/m3 and the standard deviation of 2.15×1012/m3. All data is analyzed according to the “ideal" plasma conditions of Maxwellian distributions.
Keywords: Langmuir Probe, Plasma parameters, Ion thruster, Backflow region.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1948592 Numerical Study of Mixed Convection Coupled to Radiation in a Square Cavity with a Lid-Driven
Authors: Mohamed Amine Belmiloud, Nord Eddine Sad Chemloul
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In this study, we investigated numerically heat transfer by mixed convection coupled to radiation in a square cavity; the upper horizontal wall is movable. The purpose of this study is to see the influence of the emissivity ε and the varying of the Richardson number Ri on the variation of average Nusselt number Nu. The vertical walls of the cavity are differentially heated, the left wall is maintained at a uniform temperature higher than the right wall, and the two horizontal walls are adiabatic. The finite volume method is used for solving the dimensionless Governing Equations. Emissivity values used in this study are ranged between 0 and 1, the Richardson number in the range 0.1 to 10. The Rayleigh number is fixed to Ra=104 and the Prandtl number is maintained constant Pr=0.71. Streamlines, isothermal lines and the average Nusselt number are presented according to the surface emissivity. The results of this study show that the Richardson number Ri and emissivity ε affect the average Nusselt number.Keywords: Numerical study, mixed convection, square cavity, wall emissivity, lid-driven.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2241591 Application Methodology for the Generation of 3D Thermal Models Using UAV Photogrammety and Dual Sensors for Mining/Industrial Facilities Inspection
Authors: Javier Sedano-Cibrián, Julio Manuel de Luis-Ruiz, Rubén Pérez-Álvarez, Raúl Pereda-García, Beatriz Malagón-Picón
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Structural inspection activities are necessary to ensure the correct functioning of infrastructures. UAV techniques have become more popular than traditional techniques. Specifically, UAV Photogrammetry allows time and cost savings. The development of this technology has permitted the use of low-cost thermal sensors in UAVs. The representation of 3D thermal models with this type of equipment is in continuous evolution. The direct processing of thermal images usually leads to errors and inaccurate results. In this paper, a methodology is proposed for the generation of 3D thermal models using dual sensors, which involves the application of RGB and thermal images in parallel. Hence, the RGB images are used as the basis for the generation of the model geometry, and the thermal images are the source of the surface temperature information that is projected onto the model. Mining/industrial facilities representations that are obtained can be used for inspection activities.
Keywords: Aerial thermography, data processing, drone, low-cost, point cloud.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 341590 Investigating the Effects of Hydrogen on Wet Cement for Underground Hydrogen Storage Applications in Oil and Gas Wells
Authors: Hamoud Al-Hadrami, Hossein Emadi, Athar Hussain
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Green hydrogen is quickly emerging as a new source of the renewable energy for the world. Hydrogen production using water electrolysis is deemed as an environmentally friendly and safe source of energy for transportation and other industries. However, storing high volumes of hydrogen seems to be a significant challenge. Abandoned hydrocarbon reservoirs are considered as viable hydrogen storage options because of the availability of the required infrastructure such as wells and surface facilities. However, long-term wellbore integrity in these wells could be a serious challenge. The aim of this research is to investigate the effect of stored hydrogen on the wellbore integrity such as casing cement. The methodology is to experimentally expose hydrogen to wet and dry cement and measure the impact on cement rheological and mechanical properties. Hydrogen reduces the compressive strength of a set cement if it gets in contact with the cement slurry. Also, mixing hydrogen with cement slurry slightly increases its density and rheological properties which need to be considered to have a successful primary cementing operation.
Keywords: Green hydrogen, underground storage, wellbore integrity, cement, compressive strength.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 643589 Analysis of the Coupled Stretching Bending Problem of Stiffened Plates by a BEM Formulation Based on Reissner's Hypothesis
Authors: Gabriela R. Fernandes, Danilo H. Konda, Luiz C. F. Sanches
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In this work, the plate bending formulation of the boundary element method - BEM, based on the Reissner?s hypothesis, is extended to the analysis of plates reinforced by beams taking into account the membrane effects. The formulation is derived by assuming a zoned body where each sub-region defines a beam or a slab and all of them are represented by a chosen reference surface. Equilibrium and compatibility conditions are automatically imposed by the integral equations, which treat this composed structure as a single body. In order to reduce the number of degrees of freedom, the problem values defined on the interfaces are written in terms of their values on the beam axis. Initially are derived separated equations for the bending and stretching problems, but in the final system of equations the two problems are coupled and can not be treated separately. Finally are presented some numerical examples whose analytical results are known to show the accuracy of the proposed model.
Keywords: Boundary elements, Building floor structures, Platebending.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1982588 Contact Angle Measurement of the Vinyl Ester Matrix Nanocomposites Based On Layered Silicate
Authors: A. I. Alateyah, H. N. Dhakal, Z. Y. Zhang
Abstract:
Contact angle measurement was utilized in order to study the subject of the wettability and surface chemistry of the nanocomposites materials. Water and glycerol droplets were used in this study. The incorporation of layered silicate into the vinyl ester matrix helped to improve the wettability and reduced the θ values of both liquids used. The addition of 2 wt.% clay loading reduced the θ values of water and glycerol by up to 21% and 6% respectively. Likewise, the incorporation of 4 wt.% clay loading reduced the water and glycerol θ values by 49% and 38% respectively. Also this study confirms the findings in the literature regarding the relationship between the intercalation nanocomposites level and the wettability. Wide Angle X-ray Diffraction, Scanning Electron Microscopy and Transmission Electron Microscopy were utilised in order to characterise the interlamellar structure of nanocomposites.
Keywords: Vinyl ester, nanocomposites, layered silicate, characterisations, contact angle measurement, wettability.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2123587 Analyses of Wear Mechanisms Occurring During Machining of the Titanium Alloy Ti- 6Al-2Sn-4Zr-6Mo
Authors: Z. Rihova, K. Saksl, C. Siemers, D. Ostroushko
Abstract:
Titanium alloys like the modern alloy Ti 6Al 2Sn 4Zr 6Mo (Ti-6246) combine excellent specific mechanical properties and corrosion resistance. On the other hand,due to their material characteristics, machining of these alloys is difficult to perform. The aim of the current study is the analyses of wear mechanisms of coated cemented carbide tools applied in orthogonal cutting experiments of Ti-6246 alloy. Round bars were machined with standard coated tools in dry conditions on a CNC latheusing a wide range of cutting speeds and cutting depths. Tool wear mechanisms were afterwards investigated by means of stereo microscopy, optical microscopy, confocal microscopy and scanning electron microscopy. Wear mechanisms included fracture of the tool tip (total failure) and abrasion. Specific wear features like crater wear, micro cracks and built-up edgeformation appeared depending of the mechanical and thermal conditions generated in the workpiece surface by the cutting action.
Keywords: Alloy 6246, machining, tool wear, optical microscopy, SEM, EDX analysis
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1775