Commenced in January 2007
Paper Count: 31103
Improved Technique of Non-viral Gene Delivery into Cancer Cells
Abstract:Liposomal magnetofection is a simple, highly efficient technology for cell transfection, demonstrating better outcome than a number of other common gene delivery methods. However, aggregate complexes distribution over the cell surface is non-uniform due to the gradient of the permanent magnetic field. The aim of this study was to estimate the efficiency of liposomal magnetofection for prostate carcinoma PC3 cell line using newly designed device, “DynaFECTOR", ensuring magnetofection in a dynamic gradient magnetic field. Liposomal magnetofection in a dynamic gradient magnetic field demonstrated the highest transfection efficiency for PC3 cells – it increased for 21% in comparison with liposomal magnetofection and for 42% in comparison with lipofection alone. The optimal incubation time under dynamic magnetic field for PC3 cell line was 5 minutes and the optimal rotation frequency of magnets – 5 rpm. The new approach also revealed lower cytotoxic effect to cells than liposomal magnetofection.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1070631Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1366
 D. Luo & W.M Saltzman, "Enhancement of transfection by physical concentration of DNA at the cell surface," Nat Biotechnol, vol. 18, pp. 893-589, 2000.
 C. Plank, M. Anton, C. Rudolph, J. Rosenecker, F. Krötz, "Enhancing and targeting nucleic acid delivery by magnetic force," Expert. Opin. Biol. Ther., vol. 3, pp. 745-758, 2003.
 C. Plank, U. Schillinger, F. Scherer, C. Bergemann, J.S. Rémy, F. Krötz, M. Anton, J. Lausier, J. Rosenecker, "The magnetofection method: using magnetic force to enhance gene delivery," Biol. Chem., vol. 384, pp. 737-747, 2003.
 S.W. Gersting, U. Schillinger, J. Lausier, P. Nicklaus, C. Rudolph, C. Plank, D. Reinhardt, J. Rosenecker, "Gene delivery to respiratory epithelial cells by magnetofection," J Gene Med., vol. 6, pp. 913-922, 2004.
 S. Huth, J. Lausier, S.W. Gersting, C. Rudolph, C. Plank, U. Welsch, J. Rosenecker, "Insights into the mechanism of magnetofection using PEIbased magnetofectins for gene transfer," J Gene Med., vol. 6, pp. 923- 936, 2004.
 S. W. Kamau, P. O. Hassa, B. Steitz, A. Petri-Fink, H. Hofmann, M. Hofmann- Amtenbrink, B. von Rechenberg and M.O. Hottiger, "Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field," Nucleic Acids Research, vol. 34, e40, 2006.
 S.C. McBain, U. Griesenbach, S. Xenariou, A. Keramane, C.D. Batich, E.W.F.W, Alton, et al, ÔÇ×Magnetic nanoparticles as gene delivery agents: enhanced transfection in the presence of oscillating magnet arrays", Nanotechnology, vol. 19, no. 40, 2008.
 O. Mykhaylyk, Y.S. Antequera, D. Vlaskou, C. Plank, "Generation of magnetic nonviral gene transfer agents and magnetofection in vitro," Nat. Protoc., vol. 2, pp. 2391-2411, 2007.
 O. Mykhaylyk, O. Zelphati, J. Rosenecker, C. Plank, "siRNA delivery by magnetofection," Curr Opin Mol Ther., vol. 10, no. 5, pp. 493-505, 2008.
 O. Mykhaylyk, O. Zelphati, E. Hammerschmid, M. Anton, J. Rosenecker, C. Plank, "Recent advances in magnetofection and its potential to deliver siRNAs in vitro," Methods Mol Biol., vol. 487, pp. 111-146, 2009.
 O. Mykhaylyk, Y. S├ínchez-Antequera, D. Vlaskou, E. Hammerschmid, M. Anton, O. Zelphati, C. Plank, "Liposomal magnetofection," Methods Mol Biol., vol. 605, pp. 487-525, 2010.
 C.H. Lee, E.Y. Kim, K. Jeon, J.C. Tae, K.S. Lee, Y.O. Kim, M.Y. Jeong, C.W. Yun, D.K. Jeong, S.K. Cho, J.H. Kim, H.Y. Lee, K.Z. Riu, S.G. Cho, S.P. Park, "Simple, efficient, and reproducible gene transfection of mouse embryonic stem cells by magnetofection," Stem Cells Dev., vol. 17, no. 1, pp. 133-141, 2008.
 C. Fallini, G.J. Bassell, W. Rossoll, "High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function," Mol Neurodegener., vol. 5, pp. 17, 2010.
 M. Pickard and D. Chari "Enhancement of magnetic nanoparticlemediated gene transfer to astrocytes by magnetofection: effects of static and oscillating fields," Nanomedicine (Lond), vol. 5, no. 2, 2010.
 C. Sapet, N. Laurent, A. de Chevigny, L. Le Gourrierec, E. Bertosio, O. Zelphati, C. Béclin, High transfection efficiency of neural stem cells with magnetofection," Biotechniques, vol. 50, no. 3, pp. 187-189, 2011.
 D. Ribble, N.B. Goldstein, D.A. Norris, and Y.G. Shellman, "A simple technique for quantifying apoptosis in 96-well plates," BMC Biotechnology, vol.5, no. 12, pp. 5-12, 2005.