Hydrogel Based on Cellulose Acetate Used as Scaffold for Cell Growth
A hydrogel from cellulose acetate cross linked with ethylenediaminetetraacetic dianhydride (HAC-EDTA) was synthesized by our research group, and submitted to characterization and biological tests. Cytocompatibility analysis was performed by confocal microscopy using human adipocyte derived stem cells (ASCs). The FTIR analysis showed characteristic bands of cellulose acetate and hydroxyl groups and the tensile tests evidence that HAC-EDTA present a Young’s modulus of 643.7 MPa. The confocal analysis revealed that there was cell growth at the surface of HAC-EDTA. After one day of culture the cells presented spherical morphology, which may be caused by stress of the sequestration of Ca2+ and Mg2+ ions at the cell medium by HAC-EDTA, as demonstrated by ICP-MS. However, after seven days and 14 days of culture, the cells present fibroblastoid morphology, phenotype expected by this cellular type. The results give efforts to indicate this new material as a potential biomaterial for tissue engineering, in the future in vivo approach.
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 Senna AM, Novack KM, Botaro VR. Synthesis and characterization of hydrogels from cellulose acetate by esterification crosslinking with EDTA dianhydride. Carbohydr. Polym. 2014;114:260–8.
 Seliktar D. Designing Cell-Compatible Hydrogels for Biomedical Applications. Science (80-.). (Internet). 2012 (cited 2017 Feb 20); 336:1124–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22654050.
 Ratner D. Biomaterials Science: An Introduction to material in medicine. 3rd ed. Ratner BD, Hoffman AS, Shoen JF, Lemons JE, editors. Elsevier: Academic Press; 2013.
 Tibbitt MW, Anseth KS. Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol. Bioeng. 2009;103:655–63.
 Fisher S a, Tam RY, Shoichet MS. Tissue mimetics: engineered hydrogel matrices provide biomimetic environments for cell growth. Tissue Eng. Part A (Internet). 2014;20:895–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24417669.
 Li X, Kong X, Zhang Z, Nan K, Li L, Wang X, et al. Cytotoxicity and biocompatibility evaluation of N, O-carboxymethyl chitosan/oxidized alginate hydrogel for drug delivery application. Int. J. Biol. Macromol. 2012;50:1299–305.
 Razzaq A, Schmitz S, Veigel C, Molloy JE, Geeves MA, Sparrow JC. Actin Residue Glu93 Is Identified as an Amino Acid Affecting Myosin Binding. J. Biol. Chem. (Internet). 1999 (cited 2017 Feb 21); 274:28321–8. Available from: http://www.jbc.org/cgi/content/short/274/40/28321
 Censi R, Di Martino P, Vermonden T, Hennink WE. Hydrogels for protein delivery in tissue engineering. J. Control. Release. 2012;161:680–92.
 Saçak B, Certel F, Akdeniz ZD, Karademir B, Ercan F, Öz N, et al. Repair of critical size defects using bioactive glass seeded with adipose-derived mesenchymal stem cells. J. Biomed. Mater. Res. - Part B Appl. Biomater. 2016;1–7.
 Kasir R, Vernekar VN, Laurencin CT. Regenerative Engineering of Cartilage Using Adipose-Derived Stem Cells. Regen. Eng. Transl. Med. (Internet). 2015;1:42–9. Available from: http://link.springer.com/10.1007/s40883-015-0005-0.
 Kosaraju R, Rennert RC, Maan ZN, Duscher D. Adipose-Derived Stem Cell-Seeded Hydrogels Increase Endogenous Progenitor Cell Recruitment and Neovascularization in Wounds 1. Tissue Eng. Part A. 2016; 22:295–305.
 Hansen B, Jemec GBE. The mechanical properties of skin in osteogenesis imperfecta. Arch. Dermatol. (Internet). 2002 (cited 2017 Feb 22); 138:909–11. Available from: http://www.ncbi.nlm.nih.gov/pubmed/12071818.
 Domingues JA, Cherutti G, Motta AC, Hausen MA, Oliveira RTD, Silva-Zacarin ECM, et al. Bilaminar Device of Poly(Lactic-co-Glycolic Acid)/Collagen Cultured With Adipose-Derived Stem Cells for Dermal Regeneration. Artif. Organs. 2016; 40:938–49.
 Entcheva E, Bien H, Yin L, Chung CY, Farrell M, Kostov Y. Functional cardiac cell constructs on cellulose-based scaffolding. Biomaterials. 2004;25:5753–62.
 Du J, Tan E, Kim HJ, Zhang A, Bhattacharya R, Yarema KJ. Comparative evaluation of chitosan, cellulose acetate, and polyethersulfone nanofiber scaffolds for neural differentiation. Carbohydr. Polym. 2014;99:483–90.
 Senna AM, Botaro VR. Biodegradable hydrogel derived from cellulose acetate and EDTA as a reduction substrate of leaching NPK compound fertilizer and water retention in soil. J. Control. Release (Internet). Elsevier; 2017; 260:194–201. Available from: http://dx.doi.org/10.1016/j.jconrel.2017.06.009
 Mark JE. Polymer Data Handbook. Mark JE, editor. New York: Oxford University Press; 1999.
 Manschot JF, Brakkee a J. The measurement and modelling of the mechanical properties of human skin in vivo--I. The measurement. J Biomech. 1986; 19:511–5.
 Mansour JM. Biomechanics of cartilage. Wolters Kluwer Health; 2013. p. 69–83.
 Wang L, Khor E, Wee A, Lim LY. Chitosan-alginate PEC membrane as a wound dressing: Assessment of incisional wound healing. J. Biomed. Mater. Res. 2002;63:610–8.
 Wei J, Chen Y, Liu H, Du C, Yu H, Zhou Z. Thermo-responsive and compression properties of TEMPO-oxidized cellulose nanofiber-modified PNIPAm hydrogels. Carbohydr. Polym. 2016;147:201–7.
 Haugh MG, Thorpe SD, Vinardell T, Buckley CT, Kelly DJ. The application of plastic compression to modulate fibrin hydrogel mechanical properties. J. Mech. Behav. Biomed. Mater. 2012;16:66–72.
 Takeichi M, Okada TS. Experimental Cell Research 74 (1972) 51-60. 1972;74:51–60.
 Lanza RP (Robert P, Langer RS, Vacanti J. Principles of tissue engineering. Academic Press; 2000.
 Zigmond SH. Signal transduction and actin filament organization Sally H Zigmond. Curr. Opin. Cell Biol. 1996;8:66–73.
 Abed E, Moreau R. Importance of melastatin-like transient receptor potential 7 and cations (magnesium, calcium) in human osteoblast-like cell proliferation. Cell Prolif. 2007;40:849–65.