Search results for: plant cell comportment
566 Investigation Wintering And Breeding Habitat Selection by Asiatic Houbara Bustard (Chlamydotis macqueenii ) In Central Steppe of Iran
Authors: S. Aghainajafi Zadeh, M.R. Hemami., F. Heydari
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Asiatic Houbara ( Chlamydotis macqueenii ) is a flagship and vulnerable species. In-situ conservation of this threatened species demands for knowledge of its habitat selection. The aim of this study was to determine habitat variables influencing birds wintering and breeding selection in semi- arid central Iran. Habitat features of the detected nest and pellet sites were compared with paired and random plots by quantifying a number of habitat variables. In wintering habitat use at micro scale houbara selected sites where vegetation cover was significantly lower compard to control sites( p< 0.001). Areas with low number of larger plant species (p=0.03) that were not too close to a vegetation patch(p<0.001) were selected for breeding habitat.Keywords: Asiatic houbara bustard, Habitat selection, Nest, pellet.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1525565 Electrolysis Ship for Green Hydrogen Production and Possible Applications
Authors: Julian David Hunt, Andreas Nascimento
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Green hydrogen is the most environmental, renewable alternative to produce hydrogen. However, an important challenge to make hydrogen a competitive energy carrier is a constant supply of renewable energy, such as solar, wind and hydropower. Given that the electricity generation potential of these sources vary seasonally and interannually, this paper proposes installing an electrolysis hydrogen production plant in a ship and move the ship to the locations where electricity is cheap, or where the seasonal potential for renewable generation is high. An example of electrolysis ship application is to produce green hydrogen with hydropower from the North region of Brazil and then sail to the Northeast region of Brazil and generate hydrogen using excess electricity from offshore wind power. The electrolysis ship concept is interesting because it has the flexibility to produce green hydrogen using the cheapest renewable electricity available in the market.Keywords: Green hydrogen, electrolysis ship, renewable energies, seasonal variations.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 737564 Accelerated Microwave Extraction of Natural Product using the Cryogrinding
Authors: F. Benkaci-Ali, R. Mekaoui, G. Eppe, E. De Pau, J. F. Faucont
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Team distillation assisted by microwave extraction (SDAM) considered as accelerated technique extraction is a combination of microwave heating and steam distillation, performed at atmospheric pressure. SDAM has been compared with the same technique coupled with the cryogrinding of seeds (SDAM -CG). Isolation and concentration of volatile compounds are performed by a single stage for the extraction of essential oil from Cuminum cyminum seeds. The essential oils extracted by these two methods for 5 min were quantitatively (yield) and qualitatively (aromatic profile) no similar. These methods yield an essential oil with higher amounts of more valuable oxygenated compounds, and allow substantial savings of costs, in terms of time, energy and plant material. SDAM and SDAM-CG is a green technology and appears as a good alternative for the extraction of essential oils from aromatic plants.Keywords: Steam distillation, microwave extraction, Cuminum cyminum, chromatography, mass spectrometry
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2340563 A Computer Model of Language Acquisition – Syllable Learning – Based on Hebbian Cell Assemblies and Reinforcement Learning
Authors: Sepideh Fazeli, Fariba Bahrami
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Investigating language acquisition is one of the most challenging problems in the area of studying language. Syllable learning as a level of language acquisition has a considerable significance since it plays an important role in language acquisition. Because of impossibility of studying language acquisition directly with children, especially in its developmental phases, computer models will be useful in examining language acquisition. In this paper a computer model of early language learning for syllable learning is proposed. It is guided by a conceptual model of syllable learning which is named Directions Into Velocities of Articulators model (DIVA). The computer model uses simple associational and reinforcement learning rules within neural network architecture which are inspired by neuroscience. Our simulation results verify the ability of the proposed computer model in producing phonemes during babbling and early speech. Also, it provides a framework for examining the neural basis of language learning and communication disorders.Keywords: Brain modeling, computer models, language acquisition, reinforcement learning.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1590562 Effect of Adaptation Gain on system Performance for Model Reference Adaptive Control Scheme using MIT Rule
Authors: Pankaj Swarnkar, Shailendra Jain, R.K Nema
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Adaptive control involves modifying the control law used by the controller to cope with the fact that the parameters of the system being controlled change drastically due to change in environmental conditions or in system itself. This technique is based on the fundamental characteristic of adaptation of living organism. The adaptive control process is one that continuously and automatically measures the dynamic behavior of plant, compares it with the desired output and uses the difference to vary adjustable system parameters or to generate an actuating signal in such a way so that optimal performance can be maintained regardless of system changes. This paper deals with application of model reference adaptive control scheme in first order system. The rule which is used for this application is MIT rule. This paper also shows the effect of adaptation gain on the system performance. Simulation is done in MATLAB and results are discussed in detail.Keywords: Adaptive control system, Adaptation gain, MIT rule, Model reference adaptive control.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2225561 Carotenoids and Colour Before and After Storage of Organically and Conventionally Cultivated Potato Genotypes in Latvia
Authors: Irisa Murniece, Zanda Kruma, Ilze Skrabule
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Potatoes are a good source of carotenoids, which are lipophilic compounds synthesized in plastids from isoprenoids. The aim of this research was to determine the content of carotenoids in relationship with the colour of organically and conventionally cultivated potato genotypes before and after period of storage. In cooperation with the State Priekuli Plant Breeding Institute (Latvia), six potato genotypes were studied: 'Agrie dzeltenie', 'Prelma', 'Imanta', 'S-03135-10', 'S-99108-8' and 'S-01063-5'. All the genotypes were cultivated under three different conditions: organically and conventionally (two conditions). The content of carotenoids was determined by using spectrophotometer and the colour – L*a*b* system. The results of current research show that after the period of storage, carotenoid amount has increased and in conventionally cultivated potatoes it varies from 228.514 to 552.434 μg 100 g-1 while in organically cultivated potato genotypes – from 45.485 to 662.699 μg 100 g-1 FW. Colour of potato flesh was changing during storage.
Keywords: carotenoids, colour, organic and conventional cultivation, potato genotypes, storage
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1785560 Efficiency Improvements of GaAs-based Solar Cells by Hydrothermally-deposited ZnO Nanostructure Array
Authors: Chun-Yuan Huang, Chiao-Yang Cheng, Chun-Yem Huang, Yan-Kuin Su, James Chin-Lung Fang
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ZnO nanostructures including nanowires, nanorods, and nanoneedles were successfully deposited on GaAs substrates, respectively, by simple two-step chemical method for the first time. A ZnO seed layer was firstly pre-coated on the O2-plasma treated substrate by sol-gel process, followed by the nucleation of ZnO nanostructures through hydrothermal synthesis. Nanostructures with different average diameter (15-250 nm), length (0.9-1.8 μm), density (0.9-16×109 cm-2) were obtained via adjusting the growth time and concentration of precursors. From the reflectivity spectra, we concluded ordered and taper nanostructures were preferential for photovoltaic applications. ZnO nanoneedles with an average diameter of 106 nm, a moderate length of 2.4 μm, and the density of 7.2×109 cm-2 could be synthesized in the concentration of 0.04 M for 18 h. Integrated with the nanoneedle array, the power conversion efficiency of single junction solar cell was increased from 7.3 to 12.2%, corresponding to a 67% improvement.Keywords: Anti-reflection, Chemical synthesis, Solar cells, ZnO nanostructures.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1943559 Conventional Synthesis and Characterization of Zirconium Molybdate, Nd2Zr3(MoO4)9
Authors: G. Çelik Gül, F. Kurtuluş
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Rare earths containing complex metal oxides have drawn much attention due to physical, chemical and optical properties which make them feasible in so many areas such as non-linear optical materials and ion exchanger. We have researched a systematic study to obtain rare earth containing zirconium molybdate compound, characterization, investigation of crystal system and calculation of unit cell parameters. After a successful synthesis of Nd2Zr3(MoO4)9 which is a member of rare earth metal containing complex oxides family, X-ray diffraction (XRD), High Score Plus/Rietveld refinement analysis, and Fourier Transform Infrared Spectroscopy (FTIR) were completed to determine the crystal structure. Morphological properties and elemental composition were determined by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis. Thermal properties were observed via Thermogravimetric-differential thermal analysis (TG/DTA).Keywords: Nd2Zr3(MoO4)9, solid state synthesis, powder x-ray diffraction, zirconium molybdates.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1091558 Possible Role of Polyamine on Tumor Spread after Surgical Trauma
Authors: Kuniyasu Soda
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Surgical trauma seems to facilitate metastatic spread, although the underlying mechanisms are not known. Increased concentrations of polyamines (spermine and spermidine) in the blood seem to have associated with the enhanced malignant potential of cancer cells and decrease in anti-tumor immunity of cancer patients. In addition to de novo synthesis in rapidly growing cells such as normal regenerating cells and cancer cells, cells can take up polyamines from extra-cellular sources. We have shown that increased polyamine concentration results in decreases in cytokine production and expression of adhesion molecules involved in anti-tumor immunity, such as CD11a. And, immune cells in an environment with increased polyamine levels lose anti-tumor immune functions, such as lymphokine activated killer cell (LAK) activities. Because blood polyamine levels are increased in post-surgical patients, polyamine seems to have roles on post-traumatic tumor spread.
Keywords: Immune function, LAK, Polyamine, Surgical trauma.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1762557 Evaluating the Durability and Safety of Lithium-Ion Batteries in High-Temperature Desert Climates
Authors: Kenza Maher, Yahya Zakaria, Noora S. Al-Jaidah
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Temperature is a critical parameter for lithium-ion battery performance, life, and safety. In this study, four commercially available 18650 lithium-ion cells from four different manufacturers are subjected to accelerated cycle aging for up to 500 cycles at two different temperatures (25 °C and 45 °C). The cells are also calendar-aged at the same temperatures in both charged and discharged states for six months to investigate the effect of aging and temperature on capacity fade and state of health. The results showed that all battery cells demonstrated good cyclability and had a good state of health at both temperatures. However, the capacity loss and state of health of these cells are found to be dependent on the cell chemistry and aging conditions, including temperature. Specifically, the capacity loss is found to be higher at the higher aging temperature, indicating the significant impact of temperature on the aging of lithium-ion batteries.
Keywords: Lithium-ion battery, aging mechanisms, cycle aging, calendar aging.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 206556 Integrating Bioremediation and Phytoremediation to Clean up Polychlorinated Biphenyls Contaminated Soils
Authors: Petruzzelli G., Pedron F., Rosellini I., Tassi E., Gorini F., Barbafieri M.
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This work involved the use of phytoremediation to remediate an aged soil contaminated with polychlorinated biphenyls (PCBs). At microcosm scale, tests were prepared using soil samples that have been collected in an industrial area with a total PCBs concentration of about 250 μg kg-1. Medicago sativa and Lolium italicum were the species selected in this study that is used as “feasibility test" for full scale remediation. The experiment was carried out with the addition of a mixture of randomly methylatedbeta- cyclodextrins (RAMEB). At the end of the experiment analysis of soil samples showed that in general the presence of plants has led to a higher degradation of most congeners with respect to not vegetated soil. The two plant species efficiencies were comparable and improved by RAMEB addition with a final reduction of total PCBs near to 50%. With increasing the chlorination of the congeners the removal percentage of PCBs progressively decreased.Keywords: contaminated soil, feasibility test, phytoremediation, polychlorinated biphenyls
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1800555 Two-Dimensional Modeling of Spent Nuclear Fuel Using FLUENT
Authors: Imane Khalil, Quinn Pratt
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In a nuclear reactor, an array of fuel rods containing stacked uranium dioxide pellets clad with zircalloy is the heat source for a thermodynamic cycle of energy conversion from heat to electricity. After fuel is used in a nuclear reactor, the assemblies are stored underwater in a spent nuclear fuel pool at the nuclear power plant while heat generation and radioactive decay rates decrease before it is placed in packages for dry storage or transportation. A computational model of a Boiling Water Reactor spent fuel assembly is modeled using FLUENT, the computational fluid dynamics package. Heat transfer simulations were performed on the two-dimensional 9x9 spent fuel assembly to predict the maximum cladding temperature for different input to the FLUENT model. Uncertainty quantification is used to predict the heat transfer and the maximum temperature profile inside the assembly.Keywords: Spent nuclear fuel, conduction, heat transfer, uncertainty quantification.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 856554 Effect of Natural Animal Fillers on Polymer Rheology Behaviour
Authors: M. Seidl, J. Bobek, P. Lenfeld, L. Běhálek, A. Ausperger
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This paper deals with the evaluation of flow properties of polymeric matrix with natural animal fillers. Technical university of Liberec cooperates on the long-term development of “green materials“ that should replace conventionally used materials (especially in automotive industry). Natural fibres (of animal and plant origin) from all over the world are collected and adapted (drying, cutting etc.) for extrusion processing. Inside the extruder these natural additives are blended with polymeric (synthetic and biodegradable - PLA) matrix and created compound is subsequently cut for pellets in the wet way. These green materials with unique recipes are then studied and their mechanical, physical and processing properties are determined. The main goal of this research is to develop new ecological materials very similar to unfilled polymers. In this article the rheological behaviour of chosen natural animal fibres is introduced considering their shape and surface that were observed with use of SEM microscopy.Keywords: Polypropylene matrix, Green polymers, Rheology, Natural animal fibres.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2193553 Model of Continuous Cheese Whey Fermentation by Candida Pseudotropicalis
Authors: Rudy Agustriyanto, Akbarningrum Fatmawati
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The utilization of cheese whey as a fermentation substrate to produce bio-ethanol is an effort to supply bio-ethanol demand as a renewable energy. Like other process systems, modeling is also required for fermentation process design, optimization and plant operation. This research aims to study the fermentation process of cheese whey by applying mathematics and fundamental concept in chemical engineering, and to investigate the characteristic of the cheese whey fermentation process. Steady state simulation results for inlet substrate concentration of 50, 100 and 150 g/l, and various values of hydraulic retention time, showed that the ethanol productivity maximum values were 0.1091, 0.3163 and 0.5639 g/l.h respectively. Those values were achieved at hydraulic retention time of 20 hours, which was the minimum value used in this modeling. This showed that operating reactor at low hydraulic retention time was favorable. Model of bio-ethanol production from cheese whey will enhance the understanding of what really happen in the fermentation process.Keywords: Cheese whey, ethanol, fermentation, modeling.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1818552 Analysis of Production Loss on a Linear Walking Worker Line
Authors: Qian Wang, Sylvain Lassalle, Antony R. Mileham, Geraint W. Owen
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This paper mathematically analyses the varying magnitude of production loss, which may occur due to idle time (inprocess waiting time and traveling time) on a linear walking worker assembly line. Within this flexible and reconfigurable assembly system, each worker travels down the line carrying out each assembly task at each station; and each worker accomplishes the assembly of a unit from start to finish and then travels back to the first station to start the assembly of a new product. This strategy of system design attempts to combine the flexibility of the U-shaped moving worker assembly cell with the efficiency of the conventional fixed worker assembly line. The paper aims to evaluate the effect of idle time that may offset the labor efficiency of each walking worker providing an insight into the mechanism of such a flexible and reconfigurable assembly system.Keywords: Production lines, manufacturing systems, assemblysystems, walking workers.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1896551 5-Aminolevulinic Acid-Loaded Gel, Sponge Collagen to Enhance the Delivery Ability to Skin
Authors: Yi-Ping Fang, Hsien-Ting Cheng
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Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) is an alternative therapy for treating superficial cancer, especially for skin or oral cancer. ALA, a precursor of the photosensitizer protoporphyrin IX (PpIX), is present as zwitterions and hydrophilic property which make the low permeability through the cell membrane. Collagen is a traditional carrier; its molecular composed various amino acids which bear positive charge and negative charge. In order to utilize the ion-pairs with ALA and collagen, the study employed various pH values adjusting the net charge. The aim of this study was to compare a series collagen form, including solution, gel and sponge to investigate the topical delivery behavior of ALA. The in vivo confocal laser scanning microscopy (CLSM) study demonstrated that PpIX generation ability was different pattern after apply for 6 h. Gel type could generate high PpIX, and archived more deep of skin depth.Keywords: 5-Aminolevulinic acid (ALA), Collagen, Ion-pairs, Penetration behavior
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1738550 Vetiver Oil Production from Root Culture of Vetiveria zizanioides
Authors: Rizkita R. Esyanti, Iriawati, Olga Mardisadora
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Vetiver oil is secondary metabolite that accumulates in Vetiveria zizanioides roots. The aim of this study was to obtain best type of root culture which produce high amount of vetiver oil, and was similar to metabolites produce from its mother plant. Protein analysis was also conducted to detect protein, related to putative enzymes, which have a role in terpenoids synthesis in the root culture. The results showed that root culture derived from crown explant produced the best root growth. The root culture produced primary and lateral roots, ca. 40 branches. The vetiver oil produced from root culture was analyzed by using GC-MS., and the highest content of terpenoids from roots of crown explant attained 19.024%. The result of SDS PAGE showed proteins which were ±61 kD and ± 68 kD, each might be related to putative monoterpene synthase and sesquiterpenes complex, respectively.
Keywords: Protein, Root culture, Terpenoids, Vetiver oil.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3814549 Impact Assessment of Lean Practices on Social Sustainability Indicators: An Approach Using ISM Method
Authors: Aline F. Marcon, Eduardo F. da Silva, Marina Bouzon
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The impact of lean management on environmental sustainability is the research line that receives the most attention from academicians. Therefore, the social dimension of sustainable development has so far received less attention. This paper aims to evaluate the impact of intra-plant lean manufacturing practices on social sustainability indicators extracted from the Global Reporting Initiative (GRI) parameters. The method is two-phased, including MCDM approach to uncover the most relevant practices regarding social performance and Interpretive Structural Modeling (ISM) method to reveal the structural relationship among lean practices. Professionals from the academic and industrial fields answered the questionnaires. From the results of this paper, it is possible to verify that practices such as “Safety Improvement Programs”, “Total Quality Management” and “Cross-functional Workforce” are the ones which have the most positive influence on the set of GRI social indicators.
Keywords: Indicators, ISM, lean, social, sustainability.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 796548 An Investigation into the Effect of Water Quality on Flotation Performance
Authors: Edison Muzenda
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A study was carried out to determine the effect of water quality on flotation performance. The experimental test work comprised of batch flotation tests using Denver lab cell for a period of 10 minutes. Nine different test runs were carried out in triplicates to ensure reproducibility using different water types from different thickener overflows, return and sewage effluent water (process water) and portable water. The water sources differed in pH, total dissolved solids, total suspended solids and conductivity. Process water was found to reduce the concentrate recovery and mass pull, while portable water increased the concentrate recovery and mass pull. Portable water reduced the concentrate grade while process water increased the concentrate grade. It is proposed that a combination of process water and portable water supply be used in flotation circuits to balance the different effects that the different water types have on the flotation efficiency.
Keywords: Flotation, mass pull, process water, thickeneroverflows, water quality.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 4638547 The Flotation Device Designed to Treat Phosphate Rock
Authors: Z. Q. Zhang, Y. Zhang, D. L. Li
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To overcome the some shortcomings associated with traditional flotation machines and columns in collophanite flotation, a flotation device was designed and fabricated in the laboratory. A multi-impeller pump with same function as a mechanical cell was used instead of the injection sparger and circulation pump in column flotation unit. The influence of main operational parameters of the device like feed flow rate, air flow rate and impellers’ speed on collophanite flotation was analyzed. Experiment results indicate that the influence of the operational parameters were significant on flotation recovery and grade of phosphate concentrate. The best operating conditions of the device were: feed flow rate 0.62 L/min, air flow rate 6.67 L/min and impellers speed 900 rpm. At these conditions, a phosphate concentrate assaying about 30.5% P2O5 and 1% MgO with a P2O5 recovery of about 81% was obtained from a Yuan'an phosphate ore sample containing about 22.30% P2O5 and 3.2% MgO.
Keywords: Collophanite flotation, flotation columns, flotation machines, multi-impeller pump.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 814546 Novel Structural Insights of Glutamate Racemase from Mycobacterium tuberculosis through Modeling and Docking Studies
Authors: Jayashree Ramana
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An alarming emergence of multidrug-resistant strains of the tuberculosis pathogen Mycobacterium tuberculosis and continuing high worldwide incidence of tuberculosis has invigorated the search for novel drug targets. The enzyme glutamate racemase (MurI) in bacteria catalyzes the stereoconversion of L-glutamate to D-glutamate which is a component of the peptidoglycan cell wall of the bacterium. The inhibitors targeted against MurI from several bacterial species have been patented and are advocated as promising antibacterial agents. However there are none available against MurI from Mycobacterium tuberculosis, due to the lack of its threedimensional structure. This work accomplished two major objectives. First, the tertiary structure of MtMurI was deduced computationally through homology modeling using the templates from bacterial homologues. It is speculated that like in other Gram-positive bacteria, MtMurI exists as a dimer and many of the protein interactions at the dimer interface are also conserved. Second, potent candidate inhibitors against MtMurI were identified through docking against already known inhibitors in other organisms.
Keywords: Glutamate racemase, homology modeling, docking, drug resistance.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2868545 Providing a Practical Model to Reduce Maintenance Costs: A Case Study in Golgohar Company
Authors: Iman Atighi, Jalal Soleimannejad, Ahmad Akbarinasab, Saeid Moradpour
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In the past, we could increase profit by increasing product prices. But in the new decade, a competitive market does not let us to increase profit with increase prices. Therefore, the only way to increase profit will be reduce costs. A significant percentage of production costs are the maintenance costs, and analysis of these costs could achieve more profit. Most maintenance strategies such as RCM (Reliability-Center-Maintenance), TPM (Total Productivity Maintenance), PM (Preventive Maintenance) etc., are trying to reduce maintenance costs. In this paper, decreasing the maintenance costs of Concentration Plant of Golgohar Company (GEG) was examined by using of MTBF (Mean Time between Failures) and MTTR (Mean Time to Repair) analyses. These analyses showed that instead of buying new machines and increasing costs in order to promote capacity, the improving of MTBF and MTTR indexes would solve capacity problems in the best way and decrease costs.
Keywords: Golgohar Iron Ore Mining & Industrial Company, maintainability, maintenance costs, reliability-center-maintenance.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 656544 Industrial Production and Clinical Application of L-Asparaginase: A Chemotherapeutic Agent
Authors: Soni Yadav, Sitansu Kumar Verma, Jitendra Singh, Ajay Kumar
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This article comprises detail information about L-asparaginase, encompassing topic such as various sources of L-asparaginase, mechanism and properties of L-asparaginase. Also describe the production, cultivation and purification of L-asparaginase along with information about the application of L-asparaginase. L-asparaginase catalyzes the conversion reaction to convert asparagine to aspartic acid and ammonia. Asparagine is a nutritional requirement for both normal and tumor cell. Present scenario has found that L-asparaginase has been found to be a best anti tumor or antileukemic agent. In the recent years this enzyme gained application in the field of clinical research pharmacologic and food industry. It has been characterized based on the enzyme assay principle hydrolyzing L-asparagine into L-aspartic acid and ammonia. It has been observed that eukaryotic microorganisms such as yeast and filamentous fungi have a potential for L-asparaginase production. L-asparaginase has been and is still one of the most lengthily studied therapeutic enzymes by scientist and researchers worldwide.
Keywords: L-asparaginase, antitumor, solid state fermentation, chemotherapeutic.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 6916543 Molluscicidal Effects of Ageratum conyzoides and Datura stramonium on Bulinus globosus and Lymnea natalensis
Authors: Olofintoye Lawrence Kayode, Olorunniyi Omojola Felix
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Schistosomiasis is a vector-borne water-based disease transmitted by Bulinus globosus, causing haematuria in the urine of man, while fascioliasis is a trematode zoonosis infectious transmitted by Lymnaea natalensis causing liver disease in man and animals. Adult Bulinus globosus and Lymnaea natalensis were used for the experiment. Aqueous leaf extract of Ageratum conyzoides and Datura stramonium were prepared into 25, 50, 75, 100, 200 and 400 ppm concentrations. Ten snails of each species were exposed to different concentrations in triplicates, and dechlorinated water was used as control at 24h, 48h, and 72h exposure. The results revealed that 100 ppm of both plants leaves extracts indicated mortality rates between 76.7% and 100% at 24h, 48h, and 72h for both snail species. (P < 0.05). In conclusion, the extract exercised molluscicidal activity to control the snail vector at lethal doses LC50 (66.611-72.021 ppm), CI = 63.083-77.90 ppm and LC90 (92.623-102.350), CI = 87.715-110.12 ppm.
Keywords: Snail, plant leaf, aqueous extract, mortality.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 11542 Microbiological Analysis, Cytotoxic and Genotoxic Effects from Material Captured in PM2.5 and PM10 Filters Used in the Aburrá Valley Air Quality Monitoring Network (Colombia)
Authors: Carmen E. Zapata, Juan Bautista, Olga Montoya, Claudia Moreno, Marisol Suarez, Alejandra Betancur, Duvan Nanclares, Natalia A. Cano
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This study aims to evaluate the diversity of microorganisms in filters PM2.5 and PM10; and determine the genotoxic and cytotoxic activity of the complex mixture present in PM2.5 filters used in the Aburrá Valley Air Quality Monitoring Network (Colombia). The research results indicate that particulate matter PM2.5 of different monitoring stations are bacteria; however, this study of detection of bacteria and their phylogenetic relationship is not complete evidence to connect the microorganisms with pathogenic or degrading activities of compounds present in the air. Additionally, it was demonstrated the damage induced by the particulate material in the cell membrane, lysosomal and endosomal membrane and in the mitochondrial metabolism; this damage was independent of the PM2.5 concentrations in almost all the cases.Keywords: Cytotoxic, genotoxic, microbiological analysis, PM10, PM2.5.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2334541 Influence of Biofertilizers on Flower Yield and Essential Oil of Chamomile ( Matricaria chamomile L.)
Authors: M. Haj Seyed Hadi, M. Taghi Darzi, Z. Ghandeharialavijeh, GH. Riazi
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The main objective of this study was to determine the effects of vermicompost and amino acids on the qualitative and quantitative yield of chamomile. The experiment was conducted during the growing season of 2010 at the Alborz Medical Research Center. The Treatment groups consisted of vermicompost (0, 5, 10, 15 and 20 tons/ha) and the sprays of amino acids (budding stag, flowering stage, and budding + flowering stage). The experimental design was a factorial experiment based on Randomized Complete Block Design (RCBD) with three replications. The present results have shown that the highest plant height, flower head diameter, fresh and dry flower yield and significant essential oil content were obtained by using 20- ton vermicompost per hectare. Effects of amino acids were similar to those seen in vermicompost treatment and all measured traits were seen to be significant after the spray of amino acids at the budding + flowering stage).Keywords: Vermicompost, amino acids, chamomile, yield
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2222540 Properties of Bio-Phenol Formaldehyde Composites Filled with Empty Fruit Bunch Fiber
Authors: Sharifah Nabihah Syed Jaafar, Umar Adli Amran, Rasidi Roslan, Chia Chin Hua, Sarani Zakaria
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Bio-composites derived from plant fiber and/or bioderived polymer, are likely more ecofriendly and demonstrate competitive performance with petroleum based composites. In this research, the bio phenol-formaldehyde (bio-PF) was used as a matrix and oil palm empty fruit bunch fiber (EFB) as reinforcement. The matrix was synthesized via liquefaction and condensation to enhance the combination of phenol and formaldehyde, during the process. Then, the bio-PF was mixed with different percentage of EFB (5%, 10%, 15% and 20%) and molded at 180oC. The samples that viewed under scanning electron microscopy (SEM) showed an excellent wettability and interaction between EFB and matrix. Samples of 10% EFB gave the optimum properties of impact and hardness meanwhile sample 15% of EFB gave the highest reading of flexural modulus (MOE) and flexural strength (MOR). For thermal stability analysis, it was found that the weight loss and the activation energy (Ea) of the bio-composites samples were decreased as the filler content increased.
Keywords: EFB, liquefaction, phenol formaldehyde, lignin.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2117539 Antimicrobial Activity of Girardinia heterophylla
Authors: P. S. Bedi, Neayti Thakur, Balvinder Singh
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In the present study an attempt has been made to prepare the crude extracts of leaves and stem of ‘Girardinia heterophylla’ by using various solvents like petroleum ether, ethanol and double distilled water. The samples were given the code NGLS 1, NGLS 2, NGLS 3 and NGSS 1, NGSS 2 and NGSS 3 respectively. All the extracts were used to study their antimicrobial activity against gram positive bacteria e.g. Bacillus subtilis, gram negative bacteria e.g. E. coli and K. pneumonia and antifungal activity against Aspergillus niger. The results of the antimicrobial activity showed that all the crude extracts of the plant possesses antibacterial activity. Maximum antibacterial activity was shown by NGLS 2, NGLS 3 and NGSS 3 against K. pneumonia. The growth of fungus A. niger was also inhibited by all the crude extracts. Maximum inhibition was shown by NGSS 2 followed by NGSS 1.
Keywords: Girardinia heterophylla, leaves and stem extracts, antibacterial activity, antifungal activity.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2383538 Kinetics Studies on Biological Treatment of Tannery Wastewater Using Mixed Culture
Authors: G.Durai, N.Rajamohan, C.Karthikeyan, M.Rajasimman
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In this study, aerobic digestion of tannery industry wastewater was carried out using mixed culture obtained from common effluent treatment plant treating tannery wastewater. The effect of pH, temperature, inoculum concentration, agitation speed and initial substrate concentration on the reduction of organic matters were found. The optimum conditions for COD reduction was found to be pH - 7 (60%), temperature - 30ÔùªC (61%), inoculum concentration - 2% (61%), agitation speed - 150rpm (65%) and initial substrate concentration - 1560 mg COD/L (74%). Kinetics studies were carried by using Monod model, First order, Diffusional model and Singh model. From the results it was found that the Monod model suits well for the degradation of tannery wastewater using mixed microbial consortium.Keywords: Tannery, Wastewater, Biological treatment, Aerobic, Mixed culture, Kinetics.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3168537 Effect of Nano-Silver on Growth of Saffron in Flooding Stress
Authors: N. Rezvani, A. Sorooshzadeh, N. Farhadi
Abstract:
Saffron (Crocus sativus) is cultivated as spices, medicinal and aromatic plant species. At autumn season, heavy rainfall can cause flooding stress and inhibits growth of saffron. Thus this research was conducted to study the effect of silver ion (as an ethylene inhibitor) on growth of saffron under flooding conditions. The corms of saffron were soaked with one concentration of nano silver (0, 40, 80 or 120 ppm) and then planting under flooding stress or non flooding stress conditions. Results showed that number of roots, root length, root fresh and dry weight, leaves fresh and dry weight were reduced by 10 day flooding stress. Soaking saffron corms with 40 or 80 ppm concentration of nano silver rewarded the effect of flooding stress on the root number, by increasing it. Furthermore, 40 ppm of nano silver increased root length in stress. Nano silver 80 ppm in flooding stress, increased leaves dry weight.Keywords: Flooding stress, Nano-silver, Saffron.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2914