Search results for: Tian Chen

Authors: Amit Kumar, Pushpinder Singh, Parampreet Kaur, Amarpreet Kaur

Abstract:

Ranking of fuzzy numbers play an important role in decision making, optimization, forecasting etc. Fuzzy numbers must be ranked before an action is taken by a decision maker. In this paper, with the help of several counter examples it is proved that ranking method proposed by Chen and Chen (Expert Systems with Applications 36 (2009) 6833-6842) is incorrect. The main aim of this paper is to propose a new approach for the ranking of generalized trapezoidal fuzzy numbers. The main advantage of the proposed approach is that the proposed approach provide the correct ordering of generalized and normal trapezoidal fuzzy numbers and also the proposed approach is very simple and easy to apply in the real life problems. It is shown that proposed ranking function satisfies all the reasonable properties of fuzzy quantities proposed by Wang and Kerre (Fuzzy Sets and Systems 118 (2001) 375-385).

Keywords: Ranking function, Generalized trapezoidal fuzzy numbers

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Authors: Horng-Jyh Chen, Yi-Fang Chen, Chien-Liang Lin

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In this paper, the authors try to investigate the determinants of behavioral intention of the blended E-learning course for senior students at the Active Ageing University in Taiwan. Due to lower proficiency in the use of computers and less experience on learning styles of the blended E-learning course for senior students will be expected quite different from those for most young students. After more than five weeks course for two years the questionnaire survey is executed to collect data for statistical analysis in order to understand the determinants of the behavioral intention for senior students. The object of this study is at one of the Active Ageing University in Taiwan total of 84 senior students in the blended E-learning for the ceramics teaching course. The research results show that only the perceived usefulness of the blended E-learning course has significant positive relationship with the behavioral intention.

Keywords: Active Aging University, blended E-learning, ceramics teaching course, behavioral intention

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Authors: Hsiu-Pi Lin, Wen-Chen Huang, Hui-Fang Chen, Yan-Pin Ke

Abstract:

Corporate Social Responsibility (CSR) has become a new trend of business governance. Few research studies on CSR published in Taiwanese academia, especially for medical settings, we were interested in probing the relationship of CSR and financial performance in medical settings in Taiwan. The results illustrate that: (1) a time delay effect exists with a lag between CSR effort and its performance in the hospital foundation, (2) input into the internal domains of CSR will be helpful to improve employee productivity in the hospital foundation, and (3) input into the external domains of CSR will be helpful in improving financial performance in the hospital foundation. This study overviews CSR in the medical industry in Taiwan and the relationship of CSR and financial performance. Discussions of possible implications from the study results are applied to consult the CSR concept that will be transferred into a business strategy for the organization manager.

Keywords: Corporate Social Responsibility (CSR), financialperformance, hospital foundation,

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Authors: Tsung-Hsien Yu, Ya-Hsuan Chou, Ming-Wei Chen, Chi-Ming Chen, Yi-Hsuan Li

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Observations and long-term trends indicate that climate change impacts would be significant and affects Taiwan directly and severely. Taiwan engages not only in mitigation, but also in adaptation. However, there are cognitive gaps on adaptation between government and populace. Besides, a vision of zero-carbon and renewable energy 100% will be adopted in future. Therefore, the objectives of this article are to 1) hold a National Forum for knowing differences between the strategies of zero-carbon and renewable energy 100% and cognitions of general populace, and 2) plan a clear roadmap for the vision, strategy, and measures. In this forum, we set 5 group topics, 5 presumed themes, and issues mentioned review for concluding the critical issues. Finally, there are 4 strategies and 14 critical issues which correlate with the vision and strategy of government and the cognition of the general populace.

Keywords: Cognitive gap, world café, renewable energy, zero-carbon.

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Authors: Shuimiao Wan, Chao Yuan, Baoguang Tian

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In this paper, a linear mixed model which has two random effects is broken up into two models. This thesis gets the parameter estimation of the original model and an estimation’s statistical qualities based on these two models. Then many important properties are given by comparing this estimation with other general estimations. At the same time, this paper proves the analysis of variance estimate (ANOVAE) about σ2 of the original model is equal to the least-squares estimation (LSE) about σ2 of these two models. Finally, it also proves that this estimation is better than ANOVAE under Stein function and special condition in some degree.

Keywords: Linear mixed model, Random effects, Parameter estimation, Stein function.

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Authors: Jun Wan, Zehua Chen, Yingwu Chen, Zhidong Bai

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Classification is an interesting problem in functional data analysis (FDA), because many science and application problems end up with classification problems, such as recognition, prediction, control, decision making, management, etc. As the high dimension and high correlation in functional data (FD), it is a key problem to extract features from FD whereas keeping its global characters, which relates to the classification efficiency and precision to heavens. In this paper, a novel automatic method which combined Genetic Algorithm (GA) and classification algorithm to extract classification features is proposed. In this method, the optimal features and classification model are approached via evolutional study step by step. It is proved by theory analysis and experiment test that this method has advantages in improving classification efficiency, precision and robustness whereas using less features and the dimension of extracted classification features can be controlled.

Keywords: Classification, functional data, feature extraction, genetic algorithm, wavelet.

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Authors: Tian Hai, Yang Shengsheng, Jr., Wang Yi

Abstract:

Based on an analysis of the mechanism of degradation of optical characteristics of the ZnO-pigmented white paint by electron irradiation, a model of single molecular color centers is built. An equation that explains the relationship between the changes of variation of the ZnO-pigmented white paint-s spectrum absorptance and electron fluence is derived. The uncertain parameters in the equation can be calculated using the curve fitting by experimental data. The result indicates that the model can be applied to predict the degradation of optical characteristics of ZnO-pigmented white paint by electron radiation.

Keywords: ZnO-pigmented white pain, effects of electron radiation, optical characteristics degradation, prediction model.

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Authors: Chen-Yu Wang, Tsung-Chien Chen, Ming-Hui Lee, Jen-Feng Huang

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In this paper, the innovative intelligent fuzzy weighted input estimation method (FWIEM) can be applied to the inverse heat transfer conduction problem (IHCP) to estimate the unknown time-varying heat flux efficiently as presented. The feasibility of this method can be verified by adopting the temperature measurement experiment. We would like to focus attention on the heat flux estimation to three kinds of samples (Copper, Iron and Steel/AISI 304) with the same 3mm thickness. The temperature measurements are then regarded as the inputs into the FWIEM to estimate the heat flux. The experiment results show that the proposed algorithm can estimate the unknown time-varying heat flux on-line.

Keywords: Fuzzy Weighted Input Estimation Method, IHCP andHeat Flux.

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Authors: J. R. Wang, Y. Chiang, W. Y. Li, H. T. Lin, H. C. Chen, C. Shih, S. W. Chen

Abstract:

The dry-storage systems of nuclear power plants (NPPs) in Taiwan have become one of the major safety concerns. There are two steps considered in this study. The first step is the verification of the TRACE by using VSC-17 experimental data. The results of TRACE were similar to the VSC-17 data. It indicates that TRACE has the respectable accuracy in the simulation and analysis of the dry-storage systems. The next step is the application of TRACE in the dry-storage system of Kuosheng NPP (BWR/6). Kuosheng NPP is the second BWR NPP of Taiwan Power Company. In order to solve the storage of the spent fuels, Taiwan Power Company developed the new dry-storage system for Kuosheng NPP. In this step, the dry-storage system model of Kuosheng NPP was established by TRACE. Then, the steady state simulation of this model was performed and the results of TRACE were compared with the Kuosheng NPP data. Finally, this model was used to perform the safety analysis of Kuosheng NPP dry-storage system. Besides, FRAPTRAN was used tocalculate the transient performance of fuel rods.

Keywords: BWR, TRACE, FRAPTRAN, Dry-Storage.

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Authors: Qingjiang Li, Hui Xu, Haijun Liu, Xiaobo Tian

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To judge whether the memristor can be interpreted as the fourth fundamental circuit element, we propose a variable-relation criterion of fundamental circuit elements. According to the criterion, we investigate the nature of three fundamental circuit elements and the memristor. From the perspective of variables relation, the memristor builds a direct relation between the voltage across it and the current through it, instead of a direct relation between the magnetic flux and the charge. Thus, it is better to characterize the memristor and the resistor as two special cases of the same fundamental circuit element, which is the memristive system in Chua-s new framework. Finally, the definition of memristor is refined according to the difference between the magnetic flux and the flux linkage.

Keywords: Memristor, Fundamental, Variable-Relation Criterion, Memristive system

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Authors: LI qiu-min, TIAN yi-xiang, ZHANG gao-xun

Abstract:

GMDH algorithm can well describe the internal structure of objects. In the process of modeling, automatic screening of model structure and variables ensure the convergence rate.This paper studied a new GMDH model based on polynomial spline  stimation. The polynomial spline function was used to instead of the transfer function of GMDH to characterize the relationship between the input variables and output variables. It has proved that the algorithm has the optimal convergence rate under some conditions. The empirical results show that the algorithm can well forecast Consumer Price Index (CPI).

Keywords: spline, GMDH, nonparametric, bias, forecast.

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Authors: Hung-Wei Wu, Yung-Wei Chen, Yu-Fu Chen, Cheng-Yuan Hung

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This paper presents a new ultra-wideband (UWB) bandpass filter (BPF) with sharp roll-off and dual-notched bands. The filter consists of a triangle ring multi-mode resonator (MMR) with the stub-loaded resonator (SLR) for controlling the two transmission zeros at 2.8 / 11 GHz, the embedded open-circuited stub and the asymmetric tight coupled input/output (I/O) lines for introducing the dual notched bands at 5.2 / 6.8 GHz. The attenuation slope in the lower and higher passband edges of the proposed filter show 160- and 153-dB/GHz, respectively. This study mainly provides a simple method to design a UWB bandpass filter with high passband selectivity and dual-notched bands for satisfying the Federal Communications Commission (FCC-defined) indoor UWB specification

Keywords: steeply slopes transition band, bandpass filter, ultra-wideband (UWB), triangle-ring, multi-mode resonator, stub-loaded resonator.

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Authors: Po-Yun Chen, Jui-Liang Yen, Chang-Ping Chang, Wen-Hua Hu, Yu-Liang Chen, Yih-Ming Liu, Chin-Yi Chou, Ming-Der Ger

Abstract:

The stab resistance performance of newly developed fabric composites composed of hexagonal paper honeycombs, filled with shear thickening fluid (STF), and woven Kevlar® fabric or UHMPE was investigated in this study. The STF was prepared by dispersing submicron SiO2 particles into polyethylene glycol (PEG). Our results indicate that the STF-Kevlar composite possessed lower penetration depth than that of neat Kevlar. In other words, the STF-Kevlar composite can attain the same energy level in stab-resistance test with fewer layers of Kevlar fabrics than that of the neat Kevlar fabrics. It also indicates that STF can be used for the fabrication of flexible body armors and can provide improved protection against stab threats. We found that the stab resistance of the STF-Kevlar composite increases with the increase of SiO2 concentration in STF. Moreover, the silica particles functionalized with silane coupling agent can further improve the stab resistance.

Keywords: shear thickening fluid, SiO2, Kevlar, stab

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Authors: C. C. Chen, C. M. Chu, C. J. Wang, C. Y. Chen, K. J. Huang

Abstract:

Cesiumiodide with Na doping (CsI(Na)) solution or melt is easily forming three- dimension dendrites on the free surface. The defects or bobbles form inside the CsI(Na) during the solution or melt solidification. The defects or bobbles can further effect the x-ray path in the CsI(Na) crystal and decrease the scintillation characteristics of CsI(Na). In order to enhance the CsI(Na) scintillated property we made single crystal of CsI(Na) column in the anodic aluminum oxide (AAO) template by hydraulic pressure injection method. It is interesting that when CsI(Na) melt is confined in the small AAO channels, the column grow as stable single column without any dendrites. The high aspect ratio (100~10000) of AAO and nano to sub-micron channel structure which is a suitable template for single of crystal CsI(Na) formation. In this work, a new low-cost approach to fabricate scintillator crystals using anodic aluminum oxide (AAO) rather than Si is reported, which can produce scintillator crystals with a wide range of controllable size to optimize their performance in X-ray detection.

Keywords: Cesiumiodide, AAO, scintillator, crystal, X-ray.

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Authors: C. C. Chen, C. W. Hun, C. J. Wang, C. Y. Chen, J. S. Lin, K. J. Huang

Abstract:

Cesium iodide (CsI) melt was injected into anodic aluminum oxide (AAO) template and was solidified to CsI column. The controllable AAO channel size (10~500 nm) can makes CsI column size from 10 to 500 nm in diameter. In order to have a shorter light irradiate from each singe CsI column top to bottom the AAO template was coated a TiO2 nano-film. The TiO2 film acts a refraction film and makes X-ray has a shorter irradiation path in the CsI crystal making a stronger the photo-electron signal. When the incidence light irradiate from air (R=1.0) to CsI’s first surface (R=1.84) the first refraction happen, the first refraction continue into TiO2 film (R=2.88) and produces the low angle of the second refraction. Then the second refraction continue into AAO wall (R=1.78) and produces the third refraction after refractions between CsI and AAO wall (R=1.78) produce the fourth refraction. The incidence light through TiO2 filmand the first surface of CsI then arrive to the second surface of CsI. Therefore, the TiO2 film can has shorter refraction path of incidence light and increase the photo-electron conversion efficiency.

Keywords: Cesium iodide, AAO, TiO2, Refraction, X-ray.

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Authors: C.C. Chen, C.C. Wei, S.H. Chen, S.J. Hsieh, W.G. Diau

Abstract:

This paper aims to scale up Dye-sensitized Solar Cell (DSSC) production using a commonly available industrial material – stainless steel - and industrial plasma equipment. A working DSSC electrode formed by (1) coating titania nanotube (TiO2 NT) film on 304 stainless steel substrate using a plasma spray technique; then, (2) filling the nano-pores of the TiO2 NT film using a TiF4 sol-gel method. A DSSC device consists of an anode absorbed photosensitive dye (N3), a transparent conductive cathode with platinum (Pt) nano-catalytic particles adhered to its surface, and an electrolytic solution sealed between the anode and the transparent conductive cathode. The photo-current conversion efficiency of the DSSC sample was tested under an AM 1.5 Solar Simulator. The sample has a short current (Isc) of 0.83 mA cm-2, open voltage (Voc) of 0.81V, filling factor (FF) of 0.52, and conversion efficiency (η) of 2.18% on a 0.16 cm2 DSSC work-piece.

Keywords: DSSC, Spray, stainless steel, TiO2 NT, efficiency

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Authors: Chih-Hung Wu, Chih-Chin Lai, Chun-Yen Chen, Yan-He Chen

Abstract:

Interpretation of aerial images is an important task in various applications. Image segmentation can be viewed as the essential step for extracting information from aerial images. Among many developed segmentation methods, the technique of clustering has been extensively investigated and used. However, determining the number of clusters in an image is inherently a difficult problem, especially when a priori information on the aerial image is unavailable. This study proposes a support vector machine approach for clustering aerial images. Three cluster validity indices, distance-based index, Davies-Bouldin index, and Xie-Beni index, are utilized as quantitative measures of the quality of clustering results. Comparisons on the effectiveness of these indices and various parameters settings on the proposed methods are conducted. Experimental results are provided to illustrate the feasibility of the proposed approach.

Keywords: Aerial imaging, image segmentation, machine learning, support vector machine, cluster validity index

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Authors: Shou-Chen Lo, Chia-Ching Lin, Chieh-Chen Huang

Abstract:

To decompose organochlorides by bioremediation, co-culture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78% after 16 hours, however, the cytochrome P450cam system expressed strain, CGA-camCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3-chlorobenzoate, as sole carbon source or present benzoate as co-substrate, CGA-camCAB presented faster growth rate than vector control strain.

Keywords: cytochrome P450, halorespiration, nitrogen fixation, Rhodopseudomonas palustris CGA009

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Authors: Yan Liu, Weidong Shen, Dekang Mao, Baolin Tian

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The MFCAV Riemann solver is practically used in many Lagrangian or ALE methods due to its merit of sharp shock profiles and rarefaction corners, though very often with numerical oscillations. By viewing it as a modification of the WWAM Riemann solver, we apply the MFCAV Riemann solver to the Lagrangian method recently developed by Maire. P. H et. al.. The numerical experiments show that the application is successful in that the shock profiles and rarefaction corners are sharpened compared with results obtained using other Riemann solvers. Though there are still numerical oscillations, they are within the range of the MFCAV applied in onther Lagrangian methods.

Keywords: Cell-centered Lagrangian method, approximated Riemann solver, HLLC Riemann solver

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Authors: Kuen-Long Leu, Yung-Yuan Chen, Chin-Long Wey, Jwu-E Chen, Chung-Hsien Hsu

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The increasing importance of FlexRay systems in automotive domain inspires unceasingly relative researches. One primary issue among researches is to verify the reliability of FlexRay systems either from protocol aspect or from system design aspect. However, research rarely discusses the effect of network topology on the system reliability. In this paper, we will illustrate how to model the reliability of FlexRay systems with various network topologies by a well-known probabilistic reasoning technology, Bayesian Network. In this illustration, we especially investigate the effectiveness of error containment built in star topology and fault-tolerant midpoint synchronization algorithm adopted in FlexRay communication protocol. Through a FlexRay steer-by-wire case study, the influence of different topologies on the failure probability of the FlexRay steerby- wire system is demonstrated. The notable value of this research is to show that the Bayesian Network inference is a powerful and feasible method for the reliability assessment of FlexRay systems.

Keywords: Bayesian Network, FlexRay, fault tolerance, network topology, reliability.

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Authors: Shuimiao Wan, Chao Yuan, Baoguang Tian

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In statistics parameter theory, usually the parameter estimations have two kinds, one is the least-square estimation (LSE), and the other is the best linear unbiased estimation (BLUE). Due to the determining theorem of minimum variance unbiased estimator (MVUE), the parameter estimation of BLUE in linear model is most ideal. But since the calculations are complicated or the covariance is not given, people are hardly to get the solution. Therefore, people prefer to use LSE rather than BLUE. And this substitution will take some losses. To quantize the losses, many scholars have presented many kinds of different relative efficiencies in different views. For the linear weighted regression model, this paper discusses the relative efficiencies of LSE of β to BLUE of β. It also defines two new relative efficiencies and gives their lower bounds.

Keywords: Linear weighted regression, Relative efficiency, Lower bound, Parameter estimation.

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Authors: Yung-Tan Lee, Ren-Yi Huang, Ju-Chin Chen, Jyh-Yi Shih, Meng-Lung Lin, Hsiao-Ling Yu, Yen-Tsui Hu, Chih-Cheng Chen

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Cenozoic basalts found in Jiangsu province of eastern China include tholeiites and alkali basalts. The present paper analyzed the major, trace elements, rare earth elements of these Cenozoic basalts and combined with Sr-Nd isotopic compositions proposed by Chen et al. (1990)[1] in the literatures to discuss the petrogenesis of these basalts and the geochemical characteristics of the source mantle. Based on major, trace elements and fractional crystallization model established by Brooks and Nielsen (1982)[2] we suggest that the basaltic magma has experienced olivine + clinopyroxene fractionation during its evolution. The chemical compositions of basaltic rocks from Jiangsu province indicate that these basalts may belong to the same magmatic system. Spidergrams reveal that Cenozoic basalts from Jiangsu province have geochemical characteristics similar to those of ocean island basalts(OIB). The slight positive Nb and Ti anomalies found in basaltic rocks of this study suggest the presence of Ti-bearing minerals in the mantle source and these Ti-bearing minerals had contributed to basaltic magma during partial melting, indicating a metasomatic event might have occurred before the partial melting. Based on the Sr vs. Nd isotopic ratio plots, we suggest that Jiangsu basalts may be derived from partial melting of mantle source which may represent two-end members mixing of DMM and EM-I. Some Jiangsu basaltic magma may be derived from partial melting of EM-I heated by the upwelling asthenospheric mantle or asthenospheric diapirism.

Keywords: Geochemistry, Jiangsu Province, Cenozoic basalts, Fractional crystallization.

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Authors: Ko-Shao Chen, Yun Tsao, Chia-Hsuan Tsen, Chien-Chung Chen, Shu-Chuan Liao

Abstract:

Electrospun microtube array membranes (MTAMs) made of PLLA (poly-L-lactic acid) have wide potential applications in tissue engineering. However, their surface hydrophobicity and poor biocompatability have limited their further usage. In this study, the surface of PLLA MTAMs were made hydrophilic by introducing extra functional groups, such as peroxide, via an acetic acid plasma (AAP). UV-graft polymerization of acrylic acid (G-AAc) was then used to produce carboxyl group on MTAMs surface, which bonded covalently with chitosan through EDC / NHS crosslinking agents. To evaluate the effects of the surface modification on PLLA MTAMs, water contact angle (WCA) measurement and cell compatibility tests were carried out. We found that AAP treated electrospun PLLA MTAMs grafted with AAc and, finally, with chitosan immobilized via crosslinking agent, exhibited improved hydrophilic and cell compatibility.

Keywords: Plasma, EDC/NHS, UV grafting, chitosan, microtube array membrane.

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Authors: Kevin Fong-Rey Liu, Jia-Shen Chen, Han-Hsi Liang, Cheng-Wu Chen, Yung-Shuen Shen

Abstract:

The purpose of determining impact significance is to place value on impacts. Environmental impact assessment review is a process that judges whether impact significance is acceptable or not in accordance with the scientific facts regarding environmental, ecological and socio-economical impacts described in environmental impact statements (EIS) or environmental impact assessment reports (EIAR). The first aim of this paper is to summarize the criteria of significance evaluation from the past review results and accordingly utilize fuzzy logic to incorporate these criteria into scientific facts. The second aim is to employ data mining technique to construct an EIS or EIAR prediction model for reviewing results which can assist developers to prepare and revise better environmental management plans in advance. The validity of the previous prediction model proposed by authors in 2009 is 92.7%. The enhanced validity in this study can attain 100.0%.

Keywords: Environmental impact assessment review, impactsignificance, fuzzy logic, data mining, classification tree.

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Authors: Ming-Lun Chiang, Hsi-Chia Chen, Chieh Wu, Yu-Ting Tseng, Ming-Ju Chen

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In this study, three strains of Vibrio parahaemolyticus (690, BCRC 13023 and BCRC 13025) were subjected to acid adaptation at pH 5.5 for 90 min. The survival of acid-adapted and non-adapted V. parahaemolyticus strains under simulated gastric condition and their protein expression profiles were investigated. Results showed that acid adaptation increased the survival of the test V. parahaemolyticus strains after exposure to simulated gastric juice (pH 3). Additionally, acid adaptation also affected the protein expression in these V. parahaemolyticus strains. Nine proteins, identified as atpA, atpB, DnaK, GroEL, OmpU, enolase, fructose-bisphosphate aldolase, phosphoglycerate kinase and triosephosphate isomerase, were induced by acid adaptation in two or three of the test strains. These acid-adaptive proteins may play important regulatory roles in the acid tolerance response (ATR) of V. parahaemolyticus.

Keywords: Acid adaptation, protein expression, simulated gastric juice, Vibrio parahaemolyticus

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Authors: Chin-Pin Chen, David W.S. Tai, Wen-Jong Chen, Hui-Min Lai

Abstract:

This study aims to analyze the effect of e-learning on photonics technology and daily livings among college students. The course contents of photonics technology and daily livings are first drafted based on research discussions and expert interviews. Having expert questionnaires with Delphi Technique for three times, the knowledge units and items for the course of photonics technology and daily livings are established. The e-learning materials and the drafts of instructional strategies, academic achievement, and learning attitude scales are then developed. With expert inspection, reliability and validity test, and experimental instructions, the scales and the material are further revised. Finally, the formal instructions are implemented to test the effect of different instructional methods on the academic achievement of photonics technology and daily livings among students in universities of technology. The research results show that e-learning could effectively promote academic achievement and learning attitude, and the students with e-learning obviously outperform the ones with trandition instructions.

Keywords: E-learning, Photonics Technology and Daily Livings, Academic Achievement

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Authors: Chiu-Wen Chen, Chih-Feng Chen, Cheng-Di Dong

Abstract:

Surface sediment samples were collected from the Canon River mouth, Taiwan and analyzed for polycyclic aromatic hydrocarbons (PAHs). Total PAHs concentrations varied from 337 to 1,252 ng/g dry weight, with a mean concentration of 827 ng/g dry weight. The spatial distribution of PAHs reveals that the PAHs concentration is relatively high in the river mouth region, and gradually diminishes toward the harbor region. Diagnostic ratios showed that the possible source of PAHs in the Canon River mouth could be petroleum combustion. The toxic equivalent concentrations (TEQcarc) of PAHs varied from 47 to 112 ng TEQ/g dry weight. Higher total TEQcarc values were found in the river mouth region. As compared with the US Sediment Quality Guidelines (SQGs), the observed levels of PAHs at Canon River mouth were lower than the effects range low (ERL), and would probably not exert adverse biological effects.

Keywords: PAHs, sediment, river mouth, sediment quality guidelines (SQGs), toxic equivalent (TEQcarc)

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Authors: Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu

Abstract:

Objective: Biliary atresia (BA) is characterized by progressive liver fibrosis. Epithelial-mesenchymal transition (EMT) has been implicated as a key mechanism in the pathogenesis of organ fibrosis. MiR-200a has been shown to repress EMT. We aim to explore the role of miR-200a in the fibrogenesis of BA. Methods: We obtained the plasma samples and liver samples from patients with BA or controls to examine the role of miR-200a. Histological liver fibrosis was assessed using the Ishak fibrosis scores. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to detect the expression of miR-200a in plasma. We also evaluated the expression of miR-200a in liver tissues using tyramide signal amplification fluorescence in situ hybridization (TSA-FISH). The expression of EMT related proteins zinc finger E-box-binding homeobox 1 (ZEB1), E-cadherin and α-smooth muscle actin (α-SMA) in the liver sections were detected by immunohistochemical staining. Results: We found that the expression of miR-200a was both elevated in the plasma and liver tissues from BA patients compared with the controls. The hepatic expression of ZEB1 and α-SMA were markedly increased in the liver sections from BA patients compared to the controls, whereas E-cadherin was downregulated in the BA group. Simultaneously, we noted that the hepatic expression of miR-200a, E-cadherin and α-SMA were upregulated with the progression of liver fibrosis in the BA group, while ZEB1 was downregulated with the progression of liver fibrosis in BA patients. Conclusion: These findings suggest EMT has a critical effect on the fibrotic process of BA, and the interaction between miR-200a and ZEB1 may regulate EMT and eventually influence liver fibrogenesis of BA.

Keywords: Biliary atresia, liver fibrosis, MicroRNA, epithelial-mesenchymal transition, zinc finger e-box-binding homeobox 1.

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Authors: Sin J. Chen, Jyh J. Chen

Abstract:

This study describes a micro device integrated with multi-chamber for polymerase chain reaction (PCR) with different annealing temperatures. The device consists of the reaction polydimethylsiloxane (PDMS) chip, a cover glass chip, and is equipped with cartridge heaters, fans, and thermocouples for temperature control. In this prototype, commercial software is utilized to determine the geometric and operational parameters those are responsible for creating the denaturation, annealing, and extension temperatures within the chip. Two cartridge heaters are placed at two sides of the chip and maintained at two different temperatures to achieve a thermal gradient on the chip during the annealing step. The temperatures on the chip surface are measured via an infrared imager. Some thermocouples inserted into the reaction chambers are used to obtain the transient temperature profiles of the reaction chambers during several thermal cycles. The experimental temperatures compared to the simulated results show a similar trend. This work should be interesting to persons involved in the high-temperature based reactions and genomics or cell analysis.

Keywords: Polymerase chain reaction, thermal cycles, temperature gradient, micro-fabrication.

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Authors: Yen-Cheng Chen, Fu-Chen Yang

Abstract:

IETF defines mobility support in IPv6, i.e. MIPv6, to allow nodes to remain reachable while moving around in the IPv6 internet. When a node moves and visits a foreign network, it is still reachable through the indirect packet forwarding from its home network. This triangular routing feature provides node mobility but increases the communication latency between nodes. This deficiency can be overcome by using a Binding Update (BU) scheme, which let nodes keep up-to-date IP addresses and communicate with each other through direct IP routing. To further protect the security of BU, a Return Routability (RR) procedure was developed. However, it has been found that RR procedure is vulnerable to many attacks. In this paper, we will propose a lightweight RR procedure based on geometric computing. In consideration of the inherent limitation of computing resources in mobile node, the proposed scheme is developed to minimize the cost of computations and to eliminate the overhead of state maintenance during binding updates. Compared with other CGA-based BU schemes, our scheme is more efficient and doesn-t need nonce tables in nodes.

Keywords: Mobile IPv6, Binding update, Geometric computing.

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