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Effect of Acid Adaptation on the Survival of Three Vibrio parahaemolyticus Strains under Simulated Gastric Condition and their Protein Expression Profiles

Authors: Ming-Lun Chiang, Hsi-Chia Chen, Chieh Wu, Yu-Ting Tseng, Ming-Ju Chen

Abstract:

In this study, three strains of Vibrio parahaemolyticus (690, BCRC 13023 and BCRC 13025) were subjected to acid adaptation at pH 5.5 for 90 min. The survival of acid-adapted and non-adapted V. parahaemolyticus strains under simulated gastric condition and their protein expression profiles were investigated. Results showed that acid adaptation increased the survival of the test V. parahaemolyticus strains after exposure to simulated gastric juice (pH 3). Additionally, acid adaptation also affected the protein expression in these V. parahaemolyticus strains. Nine proteins, identified as atpA, atpB, DnaK, GroEL, OmpU, enolase, fructose-bisphosphate aldolase, phosphoglycerate kinase and triosephosphate isomerase, were induced by acid adaptation in two or three of the test strains. These acid-adaptive proteins may play important regulatory roles in the acid tolerance response (ATR) of V. parahaemolyticus.

Keywords: Acid adaptation, protein expression, simulated gastric juice, Vibrio parahaemolyticus

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1076588

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References:


[1] M. H. Brown, and I. R. Booth. Acidulants and low pH. In: Russell, N. J., Gould, G. W. (Eds.), Food preservatives. Blackie, Glasgow, Scotland. 1991, pp. 22-43.
[2] S. Bearson, B. Bearson, and J. W. Foster. Acid stress responses in enterobacteria. FEMS Microbiol. Lett. 1997, 147: 173-180.
[3] J. P. Audia, C. C. Webb, and J. W. Foster. Breaking through the acid barrier: An orchestrated response to proton stress by enteric bacteria. Int. J. Med. Microbiol. 2001, 291: 97-106.
[4] J. L. Smith. The role of gastric acid in preventing foodborne disease and how bacteria overcome acid conditions. J. Food Prot. 2003, 66: 1292-1303.
[5] N. Browne, and B. C. A. Dowds. Acid stress in the food pathogen Bacillus cereus. J. Appl. Microbiol. 2002, 92: 404-414.
[6] W. Bang, and M. A. Drake. Acid adaptation of Vibrio vulnificus and subsequent impact on stress tolerance. Food Microbiol. 2005, 22: 301-309.
[7] A. Álvarez-Ordóñez, A. Fernández, A. Bernardo, and M. López. Comparison of acids on the induction of an acid tolerance response in Salmonella typhimurium, consequences for food safety. Meat Sci. 2009, 81: 65-70.
[8] H. G. Yuk, and D. L. Marshall. Adaptation of Escherichia coli O157:H7 to pH alters membrane lipid composition, verotoxin secretion, and resistance to simulated gastric fluid acid. Appl. Environ. Microbiol. 2004, 70: 3500-3505.
[9] S. M. Arvizu-Medrano, and E. F. Escartín. Effect of acid shock with hydrochloric, citric and lactic acid on the survival and growth of Salmonella Typhi and Salmonella Typhimurium in acidified media. J. Food Prot. 2005, 68: 2047-2053.
[10] P. N. Skandamis, Y. Yoon, J. D. Stopforth, P. A. Kendall, and J. N. Sofos. Heat and acid tolerance of Listeria monocytogenes after exposure to single and multiple sublethal stresses. Food Microbiol. 2008, 25: 294-303.
[11] J. W. Foster, and H. K. Hall. Adaptive acidification tolerance response of Salmonella typhimurium. J. Bacteriol. 1990, 172: 771-778.
[12] C. Hill, B. O'Driscoll, and I. Booth. Acid adaptation and food poisoning microorganisms. Int. J Food Microbiol. 1995, 28: 245-254.
[13] J. W. Foster. Salmonella acid shock proteins are required for the adaptive acid tolerance response. J. Bacteriol. 1991, 173: 6896-6902.
[14] J. W. Foster. The acid tolerance of Salmonella typhimurium involves transient synthesis of key acid shock proteins. J. Bacteriol. 1993, 175: 1981-1987.
[15] G. L. Tetteh, and L. R. Beuchat. Exposure of Shigella flexneri to acid stress and heat shock enhances acid tolerance. Food Microbiol. 2003, 20: 179-185.
[16] L. R. Beuchat. Vibrio parahaemolyticus: public health significance. Food Technol. 1982, 36: 80-83.
[17] J. Liston. Microbial hazards of seafood consumption. Food Technol. 1990, 44: 56-62.
[18] F. Feldhusen. The role of seafood in bacterial foodborne diseases. Microbes Infect. 2000, 2: 1651-1660.
[19] Y. C. Su, and C. Liu. Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol. 2007, 24: 549-558.
[20] H. C. Wong, S. H. Liu, L. W. Ku, I. Y. Lee, T. K. Wang, Y. S. Lee, C. L. Lee, L. P. Kuo, and D. Y. Shih. Characterization of Vibrio parahaemolyticus isolates obtained from foodborne illness outbreaks during 1992 through 1995 in Taiwan. J. Food. Prot. 2000, 63: 900-906.
[21] N. A. Bhuiyan, M. Ansaruzzaman, M. Kamruzzaman, K. Alam, N. R. Chowdhury, M. Nishibuchi, S. M. Faruque, D. A. Sack, Y. Takeda, and G. B. Nair. Prevalence of the pandemic genotype of Vibrio parahaemolyticus in Dhaka, Bangladesh, and significance of its distribution across different serotypes. J. Clin. Microbiol. 2002, 40: 284-286.
[22] Y. Hara-Kudo, K. Sugiyama, M. Nishibuchi, A. Chowdhury, J. Yatsuyanagi, Y. Ohtomo, A. Saito, H. Nagano, T. Nishina, H. Nakagawa, H. Konuma, M. Miyahara, and S. Kumagai. Prevalence of pandemic thermostable direct hemolysin-producing Vibrio parahaemolyticus O3:K6 in seafood and the coastal environment in Japan. Appl. Environ. Microbiol. 2003, 69: 3883-3891.
[23] S. Wang, H. Duan, W. Zhang, and J. W. Li. Analysis of bacterial foodborne disease outbreaks in China between 1994 and 2005. FEMS Immunol. Med. Microbiol. 2007, 51: 8-13.