Cold Plasma Surface Modified Electrospun Microtube Array Membrane for Chitosan Immobilization and Their Properties
Electrospun microtube array membranes (MTAMs) made of PLLA (poly-L-lactic acid) have wide potential applications in tissue engineering. However, their surface hydrophobicity and poor biocompatability have limited their further usage. In this study, the surface of PLLA MTAMs were made hydrophilic by introducing extra functional groups, such as peroxide, via an acetic acid plasma (AAP). UV-graft polymerization of acrylic acid (G-AAc) was then used to produce carboxyl group on MTAMs surface, which bonded covalently with chitosan through EDC / NHS crosslinking agents. To evaluate the effects of the surface modification on PLLA MTAMs, water contact angle (WCA) measurement and cell compatibility tests were carried out. We found that AAP treated electrospun PLLA MTAMs grafted with AAc and, finally, with chitosan immobilized via crosslinking agent, exhibited improved hydrophilic and cell compatibility.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1127202Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 686
 C.C. Chiu, J. Yang, C.H. Lin, C.C. Chen, “Structural tuning of electrospun nano-porous poly-l-lactic acid (PLLA) microtube array membranes (MTAMs)”, 3rd International Conference on Electrospinning 2014
 C.C. Chen, C.H. Wu, J.J. Wu, C.C. Chiu, C.H. Wong, M.L. Tsai, and H.T. Lin,” Accelerated bioethanol fermentation by using a novel yeast immobilization technique: Microtube array membrane”, Process Biochemistry 2015(tba):x-y.
 K.L. Ou, et. al., “Membranes of epitaxial-like packed, super aligned electrospun micron hollow poly (l-lactic acid) (PLLA) fibers”, European Polymer Journal, Volume 47, Issue 5, Pages 882–892, May 2011
 J.C. Yang et. al., “Formation of Highly Aligned, Single‐Layered, Hollow Fibrous Assemblies and the Fabrication of Large Pieces of PLLA Membranes”: Macromolecular Materials and Engineering, Volume 297, Issue 2, pages 115–122, February 2012
 S.B. Rao, and C.P. Sharma, “Use of chitosan as a biomaterial: studies on its safety and hemostatic potential,” J. Biomed. Mater., 34: 21–28, 1997
 K.S. Chen, S.F. Lin, Y. Ikada, Chinese J. of Medical and Biological Eng., Vol.13 No.3(1993)
 Feng-Huei Lin, Jui-Che Tsai, Tim-Mo Chen, Ko-Shau Chen, Jen-Ming Yang, Pei-Leun Kang, Te-Hsing Wu,” Fabrication and evaluation of auto-stripped tri-layer wound dressing for extensive burn injury”, Materials Chemistry and Physics, Vol.102, 15 April 2007, Pp. 152–158
 Hsin-Ming Wu, Yi-Syuan Wei, Ko-Shao Chen* and Yi-Jang Liou, “Plasma Surface Treatment and Grafting of Nano-Gold Surface for Immobilization of Chitosan”, Current Nanoscience, 2014, 10, pp.189-193
 Hung-Che Chou, Tsong-Rong Yan∗, Ko-Shao Chen, “Detecting cells on the surface of a silver electrode quartz crystal microbalance using plasma treatment and graft polymerization”, Colloids and Surfaces B: Biointerfaces 73 (2009) 244–249
 R. Zeng, Z. Wang, H. Wang, L. Chen, L. Yang, R. Qiao, L. Hu, Z. Li, “Effect of bond linkage on in vitro drug release and anti-HIV activity of chitosan-stavudine conjugates,” Macromolecular Research, 20:358-365, 2012
 K. S. Chen, C. W. Chou, S. H. Hsu, and H. R. Li, “Surface Grafting Polymerization and Crosslinking of Thermosensitive NIPAAm Hydrogels onto Plasma Pretreated Substrates and Drug Delivery Properties,” Materials Sci. Forum, V.:3267-3272, 2003