@article{(Open Science Index):https://publications.waset.org/pdf/10006259,
	  title     = {MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia},
	  author    = {Hai-Ying Liu and  Yi-Hao Chen and  Shu-Yin Pang and  Feng-Hua Wang and  Xiao-Fang Peng and  Li-Yuan Yang and  Zheng-Rong Chen and  Yi Chen and  Bing Zhu},
	  country	= {},
	  institution	= {},
	  abstract     = {Objective: Biliary atresia (BA) is characterized by progressive liver fibrosis. Epithelial-mesenchymal transition (EMT) has been implicated as a key mechanism in the pathogenesis of organ fibrosis. MiR-200a has been shown to repress EMT. We aim to explore the role of miR-200a in the fibrogenesis of BA. Methods: We obtained the plasma samples and liver samples from patients with BA or controls to examine the role of miR-200a. Histological liver fibrosis was assessed using the Ishak fibrosis scores. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to detect the expression of miR-200a in plasma. We also evaluated the expression of miR-200a in liver tissues using tyramide signal amplification fluorescence in situ hybridization (TSA-FISH). The expression of EMT related proteins zinc finger E-box-binding homeobox 1 (ZEB1), E-cadherin and α-smooth muscle actin (α-SMA) in the liver sections were detected by immunohistochemical staining. Results: We found that the expression of miR-200a was both elevated in the plasma and liver tissues from BA patients compared with the controls. The hepatic expression of ZEB1 and α-SMA were markedly increased in the liver sections from BA patients compared to the controls, whereas E-cadherin was downregulated in the BA group. Simultaneously, we noted that the hepatic expression of miR-200a, E-cadherin and α-SMA were upregulated with the progression of liver fibrosis in the BA group, while ZEB1 was downregulated with the progression of liver fibrosis in BA patients. Conclusion: These findings suggest EMT has a critical effect on the fibrotic process of BA, and the interaction between miR-200a and ZEB1 may regulate EMT and eventually influence liver fibrogenesis of BA.},
	    journal   = {International Journal of Medical and Health Sciences},
	  volume    = {11},
	  number    = {2},
	  year      = {2017},
	  pages     = {49 - 54},
	  ee        = {https://publications.waset.org/pdf/10006259},
	  url   	= {https://publications.waset.org/vol/122},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 122, 2017},
	}