Search results for: Reactive Oxygen Species.

Authors: Muhammad Naeem, Abdus Salam, Muhammad Ashraf, Muhammad Imran, Mehtab Ahmad, Muhammad Jamshed Khan, Muhammad Mazhar Ayaz, Muzaffar Ali, Arshad Ali, Memoona Qayyum Abir Ishtiaq

Abstract:

The present study was designed to demonstrate the seasonal variations in physico-chemical parameters of fish farm at Govt. Nursery Unit, Muzaffargarh, Department of Fisheries Govt. of Punjab, Pakistan for a period of eight months from January to August 2008. Water samples were collected on fifteen days basis and have been analyzed for estimation of Air temperature, Water temperature, Light penetration, pH, Total dissolved oxygen, Clouds, Carbonates, Bicarbonates, Total carbonates, Total dissolved solids, Chlorides, Calcium and Hardness. Seasonal fluctuations were observed in all the physico-chemical parameters of fish farm. The overall physicochemical parameters of fish pond water remained within the tolerable range throughout the study period.

Keywords: Freshwater, Fish farm, Water quality, Seasonal variation, Chemical factor

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Authors: Mashayekh Amir Shahriar, Akhlaghi Morteza, Rajaee Hajar, Khani Mohammad Reza, Shokri Babak

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A new and novel approach in medicine is the use of cold plasma for various applications such as sterilization blood coagulation and cancer cell treatment. In this paper a pin-to-hole plasma jet suitable for biological applications is investigated and characterized and the possibility and feasibility of cancer cell treatment is evaluated. The characterization includes power consumption via Lissajous method, thermal behavior of plasma using Infra-red camera as a novel method, Optical Emission Spectroscopy (OES) to determine the species that are generated. Treatment of leukemia cancer cells is also implemented and MTT assay is used to evaluate viability.

Keywords: Atmospheric Pressure Plasma Jet (APPJ), Plasma Medicine, Cancer cell treatment, leukemia.

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Authors: Mohammad Abdollahi

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Tomato (Lycopersicon esculentum Mill.) is one of the most popular, widely grown and the second most important vegetable crop, after potatoes. Nematodes have been identified as one of the major pests affecting tomato production throughout the world. The most destructive nematodes are the genus Meloidogyne. Most widespread and devastating species of this genus are M. incognita, M. javanica, and M. arenaria. These species can cause complete crop loss under adverse growing conditions. There are several potential methods for management of the root knot nematodes. Although the chemicals are widely used against the phytonematodes, because of hazardous effects of these compounds on non-target organisms and on the environment, there is a need to develop other control strategies. Nowadays, non-chemical measures are widely used to control the plant parasitic nematodes. Biocontrol of phytonematodes is an important method among environment-friendly measures of nematode management. There are some soil-inhabiting fungi that have biocontrol potential on phytonematodes, which can be used in nematode management program. The fungus Metarhizium anisopliae, originally is an entomopathogenic bioagent. Biocontrol potential of this fungus on some phytonematodes has been reported earlier. Recently, use of organic soil amendments as well as the use of bioagents is under special attention in sustainable agriculture. This research aimed to reduce the pesticide use in control of root-knot nematode, Meloidogyne javanica in tomato. The effects of M. anisopliae IMI 330189 and different levels of oak tree debris on M. javanica were determined. The combination effect of the fungus as well as the different rates of soil amendments was determined. Pots were filled with steam pasteurized soil mixture and the six leaf tomato seedlings were inoculated with 3000 second stage larvae of M. javanica/kg of soil. After eight weeks, plant growth parameters and nematode reproduction factors were compared. Based on the results of our experiment, combination of M. anisopliae IMI 330189 and oak debris caused more than 90% reduction in reproduction factor of nematode, at the rates of 100 and 150 g/kg soil (P ≤ 0.05). As compared to control, the reduction in number of galls was 76%. It was 86% for nematode reproduction factor, showing the significance of combined effect of both tested agents. Our results showed that plant debris can increase the biological activity of the tested bioagent. It was also proved that there was no adverse effect of oak debris, which potentially has antimicrobial activity, on antagonistic power of applied bioagent.

Keywords: Biological control, nematode management, organic soil, Quercus branti, root knot nematode, soil amendment.

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Authors: Dilip Nath

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A study has been conducted on the available aquatic invertebrates in the river Dhansiri at Dimapur site. The study confirmed that the river body composed of aquatic macroinvertebrate community in two phyla viz., Arthropods and Molluscs. Total ten species have been identified from there as the source of alternative protein food for the common people. Not only the protein source they are also the component of aquatic food chain and indicators of aquatic ecosystem. Proper management and strategies to promote the edible invertebrates can be considered as the alternative protein and alternative income source for the common people for sustainable livelihood improvement.

Keywords: Dhansiri, Dimapur, invertebrates, livelihood improvement, protein

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Authors: Samat Turganbekov, Zhakan Mol

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This article examines the emergence and development of the Kazakhstan species of humanism. The biggest challenge for Kazakhstan in terms of humanism is connected with advocating human values in parallel to promoting national interests; preserving the continuity of traditions in various spheres of life, business and culture. This should be a common goal for the entire society, the main direction for a national intelligence, and a platform for the state policy. An idea worth considering is a formation of national humanist tradition model; the challenges are adapting people to live in the context of new industrial and innovative economic conditions, keeping the balance during intensive economic development of the country, and ensuring social harmony in the society.

Keywords: Kazakh humanism, humanist tradition, national culture, spiritual and moral priority, national interest.

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Authors: Wen Po Cheng, Chi Hua Fu, Ping Hung Chen, Ruey Fang Yu

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Recovering resources from water purification sludge (WPS) have been gradually stipulated in environmental protection laws and regulations in many nations. Hence, reusing the WPS is becoming an important topic, and recovering alum from WPS is one of the many practical alternatives. Most previous research efforts have been conducted on studying the amphoteric characteristic of aluminum hydroxide for investigating the optimum pH range to dissolve the Al(III) species from WPS, but it has been lack of reaction kinetics or mechanisms related discussion. Therefore, in this investigation, water purification sludge (WPS) solution was broken by ultrasound to make particle size of reactants smaller, specific surface area larger. According to the reaction kinetics, these phenomena let the dissolved aluminum salt quantity increased and the reaction rate go faster.

Keywords: Aluminum, Acidification, Sludge, Recovery.

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Authors: Ngozi Nwogu, Godson Osueke, Mamdud Hossain, Edward Gobina

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An unconventional composite inorganic ceramic membrane capable of enhancing carbon dioxide emission decline was fabricated and tested at laboratory scale in conformism to various environmental guidelines and also to mitigate the effect of global warming. A review of the existing membrane technologies for carbon capture including the relevant gas transport mechanisms is presented. Single gas permeation experiments using silica modified ceramic membrane with internal diameter 20mm, outside diameter 25mm and length of 368mm deposited on a macro porous support was carried out to investigate individual gas permeation behaviours at different pressures at room temperature. Membrane fabrication was achieved using after a dip coating method. Nitrogen, Carbon dioxide, Argon, Oxygen and Methane pure gases were used to investigate their individual permeation rates at various pressures. Results show that the gas flow rate increases with pressure drop. However above a pressure of 3bar, CO2 permeability ratio to that of the other gases indicated control of a more selective surface adsorptive transport mechanism.

Keywords: Carbon dioxide composite inorganic membranes, permeability, transport mechanisms.

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Authors: Saeed Rezaei-Zarchi

Abstract:

Platinum oxide nanoparticles were prepared by a simple hydrothermal route and chemical reduction using carbohydrates (Fructose and sucrose) as the reducing and stabilizing agents. The crystallite size of these nanoparticles was evaluated from X-ray diffraction (XRD), atomic force microscopy (AFM) and transmission electron microscopy (TEM) and was found to be 10 nm as shown in figure 1, which is the demonstration of EM bright field and transmission electron microscopy. The effect of carbohydrates on the morphology of the nanoparticles was studied using TEM (Figure 1). The nanoparticles (100 μg/ml) were administered to the Pseudomonas Stutzeri and Lactobacillus cultures and the incubation was done at 35 oC for 24 hours. The nanocomposites exhibited interesting inhibitory as well as bactericidal activity against P. Stutzeri and and Lactobacillus species. Incorporation of nanoparticles also increased the thermal stability of the carbohydrates.

Keywords: Platinum oxide, P. Stutzeri, Lactobacillus, bactericidal effect.

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Authors: H. Al-Khalaifa, A. Al-Nasser

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The viewpoint towards the use of drugs or vaccines against avian parasitic diseases is one of the most striking challenges in avian medical parasitology. This includes many difficulties associated with drug resistance and in developing prophylactic vaccines. In many instances, the potential success of a vaccination in controlling parasitic diseases in poultry is well-documented. However, some medical, technical and financial limitations are still paramount. On the other hand, chemotherapy is not very well-recommended due to a number of medical limitations. But in the absence of an effective vaccine, drugs are used against parasitic diseases. This paper sheds light on some the advantages and disadvantages of using vaccination and drugs in controlling parasitic diseases in poultry species. The usage of chemotherapeutic drugs is discussed with some examples. Then, more light will be shed on using vaccines as a potentially effective and promising control tool.

Keywords: Drugs, parasitology, poultry, vaccines.

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Authors: Mohammad Asif

Abstract:

An accurate prediction of the minimum fluidization velocity is a crucial hydrodynamic aspect of the design of fluidized bed reactors. Common approaches for the prediction of the minimum fluidization velocities of binary-solid fluidized beds are first discussed here. The data of our own careful experimental investigation involving a binary-solid pair fluidized with water is presented. The effect of the relative composition of the two solid species comprising the fluidized bed on the bed void fraction at the incipient fluidization condition is reported and its influence on the minimum fluidization velocity is discussed. In this connection, the capability of packing models to predict the bed void fraction is also examined.

Keywords: Bed void fraction, Binary solid mixture, Minimumfluidization velocity, Packing models

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Authors: Wen Po Cheng, Chia Yun Chung, Ruey Fang Yu, Chao Feng Chen

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The main objective of this research was to study the differences of aluminum hydrolytic products between two PACl preparation methods. These two methods were the acidification process of freshly formed amorphous Al(OH)₃ and the conventional alkalization process of aluminum chloride solution. According to Ferron test and ²⁷Al NMR analysis of those two PACl preparation procedures, the reaction rate constant (k) values and Al₁₃ percentage of acid addition process at high basicity value were both lower than those values of the alkaline addition process. The results showed that the molecular structure and size distribution of the aluminum species in both preparing methods were suspected to be significantly different at high basicity value.

Keywords: Polyaluminum chloride, Al13, amorphous aluminum hydroxide, Ferron test.

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Authors: T. Atan, T. Ayyıldız, P. Akyol

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The purpose of this study was to examine and compare physical fitness values of students engaged in different team sport branches Totally 60 female, and 60 male athletes, that 20 athletes in each branch which are volleyball, basketball and football participated the study as a volunteer. The mean ages of female and male athletes were 21.20 ±1.87 and 21.61 ± 1.61 respectively. Age, height, body weight, body mass index, flexibility, body fat percentage, 30m sprint, maximum oxygen consumption capacity (MaxVO2) and drop jump values were measured. As a result of measurements, significant differences were found in height, weight, MaxVO2, shuttle run speed between different sports branches in female athletes. In male athletes, height, body weight, flexibility, 30m split speed and drop jump values were found significantly different between sports branches. As a conclusion and as a literature, it can be said that structure of body has to be appropriate with the engaged sports branch. Physical fitness values that required the sports branches can be expressed clearly by increasing the number of subjects.

Keywords: Volleyball, basketball, football, athletes, physical fitness.

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Authors: P. Sangarun, W. Srisang, K. Jaroensutasinee, M. Jaroensutasinee

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A better understanding of cloud forest characteristic in a tropical montane cloud forest at Khao Nan, Nakhon Si Thammarat on climatic, vegetation, soil and hydrology were studied during 18-21 April 2007. The results showed that as air temperature at Sanyen cloud forest increased, the percent relative humidity decreased. The amount of solar radiation at Sanyen cloud forest had a positive association with the amount of solar radiation at Parah forest. The amount of solar radiation at Sanyen cloud forest was very low with a range of 0-19 W/m2. On the other hand, the amount of solar radiation at Parah forest was high with a range of 0-1000 W/m2. There was no difference between leaf width, leaf length, leaf thickness and leaf area with increasing in elevations. As the elevations increased, bush height and tree height decreased. There was no association between bush width and bush ratio with elevation. As the elevations increased, the percent epiphyte cover and the percent soil moisture increased but water temperature, conductivity, and dissolved oxygen decreased. The percent soil moistures and organic contents were higher at elevations above 900 m than elevations below.

Keywords: Cloud forest, climate, vegetation, soil, hydrology.

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Authors: A. Bouzid, R. Chadli

Abstract:

Fritillaria oranensis (Liliaceae) was described in 1874 by pomel from Algeria. Plant samples have been collected from the mount of Tessala (Sidi-Bel-Abbes). The morphological features of various organs of the plant are described in detail. In the morphological part of the study, features of various organs of the plants such as stem and leaf were determined and illustrated. Ecological studies provide information about the physical and chemical structure of soil types in Tessala Mountain. The aim of this original investigation is to put forth ecological and anatomical features of these species for the first time, but at the same time given detailed account of the morphological characteristics of the stem and leaf of Fritillaria oranensis.

Keywords: Anatomy, ecology, Liliaceae, morphology, Fritillaria oranensis Pomel.

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Authors: Ekta Singh

Abstract:

Indices summarizing community structure are used to evaluate fundamental community ecology, species interaction, biogeographical factors, and environmental stress. Some of these indices are insensitive to gross community changes induced by contaminants of pollution. Diversity indices and similarity indices are reviewed considering their ecological application, both theoretical and practical. For some useful indices, empirical equations are given to calculate the expected maximum value of the indices to which the observed values can be related at any combination of sample sizes at the experimental sites. This paper examines the effects of sample size and diversity on the expected values of diversity indices and similarity indices, using various formulae. It has been shown that all indices are strongly affected by sample size and diversity. In some indices, this influence is greater than the others and an attempt has been made to deal with these influences.

Keywords: Biogeographical factors, Diversity Indices, Ecology and Similarity Indices

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Authors: Caixia Kang, Takahiro Kuba, Aimin Hao, Yasushi Iseri, Chunjie Li, Zhenjia Zhang

Abstract:

To improve the water quality of lakes and control algae blooms, the effects of Vallisneria asiatica which is one of aquatic plants spread over Lake Taihu, with different biomasses on the water quality and algae communities were researched. The results indicated that V. asiatica could control an excess of Microcystis spp. when the V. asiatica biomass was larger than 50g in the tank with 30L solution in the laboratory. Planktonic and epiphytic algae responded differently to V. asiatica. The presence of macrophyte V. asiatica in eutrophic waters has a positive effect on algae compositions because of different sensitivities of algae species to allelopathic substances released by macrophyte V. asiatica. That is, V. asiatica could inhibit the growth of Microcystis spp. effectively and was benefited to the diatom on the condition in the laboratory.

Keywords: Algae bloom, algae community, Microcystis spp., Vallisneria asiatica.

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Authors: Usha N. Murthy, Rekha H. B., Mahaveer Devoor

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The introduction of more stringent pollution regulations, in relation to financial and social pressures for sustainable development, has pressed toward limiting the volumes of industrial and domestic effluents discharged into the environment - as well as to increase the efforts within research and development of new or more efficient wastewater treatment technologies. Considering both discharge volume and effluent composition, wastewater generated by the textile industry is rated as the most polluting among all industrial sectors. The pollution load is mainly due to spent dye baths, which are composed of unreacted dyes, dispersing agents, surfactants, salts and organics. In the present investigation, the textile dye wastewater was characterized by high color, chemical oxygen demand (COD), total dissolved solids (TDS) and pH. Electrochemical oxidation process for four plate electrodes was carried out at five different current intensities, out of which 0.14A has achieved maximum percentage removal of COD with 75% and 83% of color. The COD removal rate in kg COD/h/m² decreases with increase in the current intensity. The energy consumption increases with increase in the current intensity. Hence, textile dye wastewater can be effectively pretreated by electrochemical oxidation method where the process limits objectionable color while leaving the COD associated with organics left for natural degradation thus causing a sustainable reduction in pollution load.

Keywords: Electrochemical treatment, COD, color.

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Authors: Boci J., Çabeli P., Shtylla T., Kumbe I.

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The development of the poultry industry in Albania is mainly based on the existence of intensive modern farms with huge capacities, which often are mixed with other forms. Colibacillosis is commonly displayed regardless of the type of breeding, delivering high mortality in poultry industry. The mechanisms with which pathogen enterobacters are able to cause the infection in poultry are not yet clear. The routine diagnose in the field, followed by isolation of E. coli and species of Salmonella genres in reference laboratories cannot lead in classification or full recognition of circulative strains in a territory, if it is not performed a differentiation among the present microorganisms in intensive farms and those in rural areas. In this study were isolated 1.496 strains of E. coli and 378 Salmonella spp. This study, presents distribution of poultry pathogenosity of E.coli and Salmonella spp., based on the usage of innovative diagnostic methods.

Keywords: poultry, E.coli, Salmonella spp., Enterobacter

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Authors: J. I. Santos, A. E. Cesarin, C. A. R. Sales, M. B. B. Triano, P. F. R. B. Martins, A. F. Braga, N. J. Neto, A., A. M. Barroso, P. L. C. A. Alves, C. A. M. Huaman

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Climate change projections based on the emission of greenhouse effect gases suggest an increase in the concentration of atmospheric carbon dioxide, in up to 750 ppm. In this scenario, we have significant changes in plant development, and consequently, in agricultural systems. This study aims to evaluate the interaction between culture (Glycine max) and weed (Amaranthus viridis and Euphorbia heterophylla) in two conditions of CO₂, 400 and 800 ppm. The results showed that the coexistence of culture with both weed species resulted in a mutual loss, with decrease in dry mass productivity of culture + weeds, in both conditions of CO₂. However, when the culture is grown in association with E. heterophylla, total dry mass of culture + weed was smaller at 800 ppm. Soybean was more aggressive in comparison to the A. viridis in both the concentrations of CO₂, but not in relation to the E. heterophylla.

Keywords: Plants interaction, increase of [CO2], plants of metabolism C3, Glycine max.

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Authors: Y. L. Yung, Kong Mun Lo

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In this study, solid phase micro-extraction (SPME) was optimized to improve the sensitivity and accuracy in formaldehyde determination for plywood panels. Further work has been carried out to compare the newly developed technique with existing method which reacts formaldehyde collected in desiccators with acetyl acetone reagent (DC-AA). In SPME, formaldehyde was first derivatized with O-(2,3,4,5,6 pentafluorobenzyl)-hydroxylamine hydrochloride (PFBHA) and analysis was then performed by gas chromatography in combination with mass spectrometry (GC-MS). SPME data subjected to various wood species gave satisfactory results, with relative standard deviations (RSDs) obtained in the range of 3.1-10.3%. It was also well correlated with DC values, giving a correlation coefficient, RSQ, of 0.959. The quantitative analysis of formaldehyde by SPME was an alternative in wood industry with great potential

Keywords: Formaldehyde, GCMS, Plywood and SPME

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Authors: Cigdem Yangin-Gomec, Aigerim Jaxybayeva, Orhan Ince

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In this study, the anaerobic treatability of chicken manure diluted with tap water (with an influent feed ratio of 1 kg of fresh chicken manure to 6 liter of tap water) was investigated in a lab-scale anaerobic sludge bed (ASB) reactor inoculated with the granular sludge already adapted to chicken manure. The raw waste digested in this study was the manure from laying-hens having average total solids (TS) of about 30% with ca. 60% volatile content. The ASB reactor was fed semi-continuously at ambient operating temperature range (17-23^◦C) at a HRT of 13 and 26 days for about 6 months, respectively. The respective average total and soluble chemical oxygen demand (COD) removals were ca. 90% and 75%, whereas average biomethane production rate was calculated ca. 180 lt per kg of COD_removed from the ASB reactor at an average HRT of 13 days. Moreover, total suspended solids (TSS) and volatile suspended solids (VSS) in the influent were reduced more than 97%. Hence, high removals of the organic compounds with respective biogas production made anaerobic stabilization of the diluted chicken manure by ASB reactor at ambient operating temperatures viable. By this way, external heating up to 35^◦C (i.e. anaerobic processes have been traditionally operated at mesophilic conditions) could be avoided in the scope of this study.

Keywords: Ambient anaerobic digestion, biogas recovery, poultry manure.

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Authors: Abdul M. A. Rahman , Foong S. Yeok, Atikah F. Amir

Abstract:

Plants are commonly known for its positive correlation in reducing temperature. Since it can benefit buildings by modifying the microclimate, it-s also believed capable of reducing the internal temperature. Various experiments have been done in Universiti Sains Malaysia, Penang to investigate the comparison in thermal benefits between two rooms, one being a typical control room (exposed wall) and the other a biofacade room (plant shaded wall). The investigations were conducted during non-rainy season for approximately a month. Climbing plant Psophocarpus tetrogonobulus from legume species was selected as insulation for the biofacade wall. Conclusions were made on whether the biofacade can be used to tackle the energy efficiency, based on the parameters taken into consideration.

Keywords: biofacade, thermal benefits, carbon sequestration, Psophocarpus tetrogonobulus.

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Authors: Mohammad Hasan Raouf, Ahmad Rouhani, Mohammad Abedini, Ebrahim Rasooli Anarmarzi

Abstract:

In this paper the application of a hierarchical fuzzy system (HFS) based on MPSS and SVC in multi-machine environment is studied. Also the effect of communication lines active power variance signal between two ΔP_Tie-line regions, as one of the inputs of hierarchical fuzzy multi-input PSS and SVC (HFMPSS & SVC), on the increase of low frequency oscillation damping is examined. In the MPSS, to have better efficiency an auxiliary signal of reactive power deviation (ΔQ) is added with ΔP+ Δω input type PSS. The number of rules grows exponentially with the number of variables in a classic fuzzy system. To reduce the number of rules the HFS consists of a number of low-dimensional fuzzy systems in a hierarchical structure. Phasor model of SVC is described and used in this paper. The performances of MPSS and ΔP_Tie-line based HFMPSS and also the proposed method in damping inter-area mode of oscillation are examined in response to disturbances. The efficiency of the proposed model is examined by simulating a four-machine power system. Results show that the proposed method is performing satisfactorily within the whole range of disturbances and reduces the cost of system.

Keywords: Communication lines active power variance signal, Hierarchical fuzzy system (HFS), Multi-input power system stabilizer (MPSS), Static VAR compensator (SVC).

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Authors: Amir Amiri, Naghmeh Morakabati

Abstract:

During the recent years the six-fold growth of cancer in Iran has led the production of healthy products to become a challenge in the food industry. Due to the young population in the country, the consumption of fast foods is growing. The chemical cancer-causing preservatives are used to produce these products more than the standard; so using an appropriate alternative seems to be important. On the one hand, the plant essential oils show the high antimicrobial potential against pathogenic and spoilage microorganisms and on the other hand they are highly volatile and decomposed under the processing conditions. The study aims to produce the loaded chitosan nanoparticles with different concentrations of savory essential oil to improve the anti-microbial property and increase the resistance of essential oil to oxygen and heat. The encapsulation efficiency was obtained in the range of 32.07% to 39.93% and the particle size distribution of the samples was observed in the range of 159 to 210 nm. The range of Zeta potential was obtained between -11.9 to -23.1 mV. The essential oil loaded in chitosan showed stronger antifungal activity against Rhizopus stolonifer. The results showed that the antioxidant property is directly related to the concentration of loaded essential oil so that the antioxidant property increases by increasing the concentration of essential oil. In general, it seems that the savory essential oil loaded in chitosan particles can be used as a food processor.

Keywords: Chitosan, encapsulation, essential oil, nanogel.

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Authors: Nilubon Kurubanjerdjit, Nattakarn Iam-On, Ka-Lok Ng

Abstract:

MicroRNAs are small non-coding RNA found in many different species. They play crucial roles in cancer such as biological processes of apoptosis and proliferation. The identification of microRNA-target genes can be an essential first step towards to reveal the role of microRNA in various cancer types. In this paper, we predict miRNA-target genes for lung cancer by integrating prediction scores from miRanda and PITA algorithms used as a feature vector of miRNA-target interaction. Then, machine-learning algorithms were implemented for making a final prediction. The approach developed in this study should be of value for future studies into understanding the role of miRNAs in molecular mechanisms enabling lung cancer formation.

Keywords: MicroRNA, miRNAs, lung cancer, machine learning, Naïve Bayes, SVM.

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Authors: A. Chaudhary, H.S. Singh

Abstract:

Present communication deals with general distribution and diversification of Monogenean families parasitizing different freshwater fish families of India. Levels of monogenean parasitism and their diversity are significantly greater in Indian fishes. The most monogeneans parasitized family of fish is Cyprinidae and most dactylogyrids parasitise cyprinids. The family dactylogyridae has more species than any other monogenean family and frequently associated with cyprinid, silurids and bagrids families. Of the various 52 families of freshwater fishes from India, only the Anguillidae, Balitoridae, Chacidae, Chanidae, Channidae, Cobitidae, Coiidae, Erethistidae, Megalopidae, Pristidae, Psilorhynchidae, Salmonidae, Schileidae, Sparidae, Synodontidae and Terapontidae were found to be free of infection with monogeneans. The present study takes a broad look at monogenean diversity in the freshwater fishes of India.

Keywords: Diversification, fish, India, Monogenea

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Authors: Ali Q. S., Farid A. J., Kabeir B. M., Zamberi S., Shuhaimi M., Ghazali H. M., Yazid A. M.

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Adhesion to the human intestinal cell is considered as one of the main selection criteria of lactic acid bacteria for probiotic use. The adhesion ability of two Bifidobacteriums strains Bifidobacterium longum BB536 and Bifidobacterium psudocatenulatum G4 was done using HT-29 human epithelium cell line as in vitro study. Four different level of pH were used 5.6, 5.7, 6.6, and 6.8 with four different times 15, 30, 60, and 120 min. Adhesion was quantified by counting the adhering bacteria after Gram staining. The adhesion of B. longum BB536 was higher than B. psudocatenulatum G4. Both species showed significant different in the adhesion properties at the factors tested. The highest adhesion for both Bifidobacterium was observed at 120 min and the low adhesion was in 15 min. The findings of this study will contribute to the introduction of new effective probiotic strain for future utilization.

Keywords: Bifidobacterium, Adhesion, HT-29 human epithelium cells.

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Authors: E. Moroydor Derun, Z. Eslek, S. Piskin

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Hypericum perforatum L. is a member of the Hypericaceae (Guttiferae) family and commonly known as St. John’s wort. There is a growing interest in this medicinal plant because of the constituents of this genus. A number of species have been shown to possess various biological activities such as antiviral, wound healing, analgesic, hepatoprotective, antimicrobial and antioxidant activities and also have therapeutic effects on burns, bruises, swelling, anxiety and mild to moderate depression. In this study, the aerial parts of Hypericum perforatum L. are extracted and the main and effective constituents are determined. The analysis of the extracts was performed by GC-MS and LC-MS. As a next step, it is aimed to investigate the usage of the main constituents of the medicinal plant.

Keywords: Hypericaceae, Hypericum perforatum L., GC-MS, Guttiferae, LC-MS, Medicinal plant, St. John’s wort.

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Authors: R. Simutis, V. Galvanauskas, D. Levisauskas, J. Repsyte, V. Grincas

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This paper deals with advanced state estimation algorithms for estimation of biomass concentration and specific growth rate in a typical fed-batch biotechnological process. This biotechnological process was represented by a nonlinear mass-balance based process model. Extended Kalman Filter (EKF) and Particle Filter (PF) was used to estimate the unmeasured state variables from oxygen uptake rate (OUR) and base consumption (BC) measurements. To obtain more general results, a simplified process model was involved in EKF and PF estimation algorithms. This model doesn’t require any special growth kinetic equations and could be applied for state estimation in various bioprocesses. The focus of this investigation was concentrated on the comparison of the estimation quality of the EKF and PF estimators by applying different measurement noises. The simulation results show that Particle Filter algorithm requires significantly more computation time for state estimation but gives lower estimation errors both for biomass concentration and specific growth rate. Also the tuning procedure for Particle Filter is simpler than for EKF. Consequently, Particle Filter should be preferred in real applications, especially for monitoring of industrial bioprocesses where the simplified implementation procedures are always desirable.

Keywords: Biomass concentration, Extended Kalman Filter, Particle Filter, State estimation, Specific growth rate.

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Authors: Jin-Hong Kim

Abstract:

Hydraulic analysis on microhabitat of Benthic Macro- invertebrates was performed at riparian riffles of Hongcheon River and Gapyeong Stream. As for the representative species, Ecdyonurus kibunensis, Paraleptophlebia cocorata, Chironomidae sp. and Psilotreta kisoensis iwata were chosen. They showed hydraulically different habitat types by flow velocity and particle diameters of streambed materials. Habitat conditions of the swimmers were determined mainly by the flow velocity rather than by flow depth or by riverbed materials. Burrowers prefer sand and silt, and inhabited at the riverbed. Sprawlers prefer cobble or boulder and inhabited for velocity of 0.05-0.15 m/s. Clingers prefer pebble or cobble and inhabited for velocity of 0.06-0.15 m/s. They were found to be determined mainly by the flow velocity.

Keywords: Benthic macroinvertebrates, riffles, clinger, swimmer, burrower, sprawler.

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