Search results for: induction motor efficiency

Authors: Padmakumar S., Vivek Agarwal, Kallol Roy

Abstract:

A new observer based fault detection and diagnosis scheme for predicting induction motors- faults is proposed in this paper. Prediction of incipient faults, using different variants of Kalman filter and their relative performance are evaluated. Only soft faults are considered for this work. The data generation, filter convergence issues, hypothesis testing and residue estimates are addressed. Simulink model is used for data generation and various types of faults are considered. A comparative assessment of the estimates of different observers associated with these faults is included.

Keywords: Extended Kalman Filter, Fault detection and diagnosis, Induction motor model, Unscented Kalman Filter

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Authors: Sarah Abdourraziq, Rachid El Bachtiri

Abstract:

The solar power source for pumping water is one of the most promising areas in photovoltaic applications. The implementation of these systems allows to protect the environment and reduce the CO₂ gas emission compared to systems trained by diesel generators. This paper presents a comparative study between the photovoltaic pumping system driven by DC motor, and AC motor to define the optimum design of this application. The studied system consists of PV array, DC-DC Boost Converter, inverter, motor-pump set and storage tank. The comparison was carried out to define the characteristics and the performance of each system. Each subsystem is modeled in order to simulate the whole system in MATLAB/ Simulink. The results show the efficiency of the proposed technique.

Keywords: Photovoltaic water pumping system, DC motor-pump, AC motor-pump, DC-DC boost converter.

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Authors: L. Moussaoui, L. Rahmani

Abstract:

This paper deals with the direct torque control (DTC) of the induction motor. This type of control allows decoupling control between the flux and the torque without the need for a transformation of coordinates. However, as with other hysteresis-based systems, the classical DTC scheme represents a high ripple, in both the electromagnetic torque and the stator flux and a distortion in the stator current. As well, it suffers from variable switching frequency. To solve these problems various modifications, in conventional DTC scheme, have been made during the last decade. Indeed the DTC based on space vector modulation (SVM) has proved to generate very low ripples in torque and flux with constant switching frequency. It also shows almost the same dynamic performances as the classical DTC system. On the other hand, fuzzy logic is considered as an interesting alternative approach for its advantages: Analysis close to the exigencies of user, ability of nonlinear systems control, best dynamic performances and inherent quality of robustness.

Therefore, two fuzzy direct torque control approaches, for the induction motor fed by SVM-voltage source inverter, are proposed in this paper. By using these two approaches of DTC, the advantages of fuzzy logic control, space vector modulation, and direct torque control method are combined. The performances of these DTC schemes are evaluated through digital simulation using Matlab/Simulink platform and fuzzy logic tools. Simulation results illustrate the effectiveness and the superiority of the proposed Fuzzy DTC-SVM schemes in comparison to the classical DTC.

Keywords: Direct torque control, Fuzzy logic control, Induction motor, Switching frequency, Space vector modulation, Torque and flux ripples.

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Authors: Ben Hamed M., Sbita L

Abstract:

This paper presents a new method for the implementation of a direct rotor flux control (DRFOC) of induction motor (IM) drives. It is based on the rotor flux components regulation. The d and q axis rotor flux components feed proportional integral (PI) controllers. The outputs of which are the target stator voltages (vdsref and vqsref). While, the synchronous speed is depicted at the output of rotor speed controller. In order to accomplish variable speed operation, conventional PI like controller is commonly used. These controllers provide limited good performances over a wide range of operations even under ideal field oriented conditions. An alternate approach is to use the so called fuzzy logic controller. The overall investigated system is implemented using dSpace system based on digital signal processor (DSP). Simulation and experimental results have been presented for a one kw IM drives to confirm the validity of the proposed algorithms.

Keywords: DRFOC, fuzzy logic, variable speed drives, control, IM and real time.

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Authors: Jagadish H. Pujar, S. F. Kodad

Abstract:

Recent developments in Soft computing techniques, power electronic switches and low-cost computational hardware have made it possible to design and implement sophisticated control strategies for sensorless speed control of AC motor drives. Such an attempt has been made in this work, for Sensorless Speed Control of Induction Motor (IM) by means of Direct Torque Fuzzy Control (DTFC), PI-type fuzzy speed regulator and MRAS speed estimator strategy, which is absolutely nonlinear in its nature. Direct torque control is known to produce quick and robust response in AC drive system. However, during steady state, torque, flux and current ripple occurs. So, the performance of conventional DTC with PI speed regulator can be improved by implementing fuzzy logic techniques. Certain important issues in design including the space vector modulated (SVM) 3-Ф voltage source inverter, DTFC design, generation of reference torque using PI-type fuzzy speed regulator and sensor less speed estimator have been resolved. The proposed scheme is validated through extensive numerical simulations on MATLAB. The simulated results indicate the sensor less speed control of IM with DTFC and PI-type fuzzy speed regulator provides satisfactory high dynamic and static performance compare to conventional DTC with PI speed regulator.

Keywords: Sensor-less Speed Estimator, Fuzzy Logic Control(FLC), SVM, DTC, DTFC, IM, fuzzy speed regulator.

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Authors: Mehdi Nasri, Hossein Nezamabadi-pour, Malihe Maghfoori

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This Paper presents a particle swarm optimization (PSO) method for determining the optimal proportional-integral-derivative (PID) controller parameters, for speed control of a linear brushless DC motor. The proposed approach has superior features, including easy implementation, stable convergence characteristic and good computational efficiency. The brushless DC motor is modelled in Simulink and the PSO algorithm is implemented in MATLAB. Comparing with Genetic Algorithm (GA) and Linear quadratic regulator (LQR) method, the proposed method was more efficient in improving the step response characteristics such as, reducing the steady-states error; rise time, settling time and maximum overshoot in speed control of a linear brushless DC motor.

Keywords: Brushless DC motor, Particle swarm optimization, PID Controller, Optimal control.

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Authors: N. Ben Si Ali, N. Benalia, N. Zarzouri

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Speed sensorless systems are intensively studied during recent years; this is mainly due to their economical benefit and fragility of mechanical sensors and also the difficulty of installing this type of sensor in many applications. These systems suffer from instability problems and sensitivity to parameter mismatch at low speed operation. In this paper an analysis of adaptive observer stability with stator resistance estimation is given.

Keywords: Motor drive, sensorless control, adaptive observer, stator resistance estimation.

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Authors: Jun-Hyuk Choi, Joon Sung Park, Jung-Moo Seo, In-Soung Jung

Abstract:

A brushless DC motor with integrated drive circuit for air management system is presented. Using magnetic equivalent circuit model a basic design of the motor is determined, and specific configurations are inspected thanks to finite element analysis. In order to reduce an unbalanced magnetic force in an axial direction, induced forces between a stator core and a permanent magnet are calculated with respect to the relative positions of them. For the high efficiency, and high power density, BLDC motor and drive are developed. Also vibration mode and eccentricity of a rotor are considered at the rated and maximum rotational speed Through the experimental results, a validity of the simulated one is confirmed.

Keywords: blower, BLDC, inverter

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Authors: Ehsan Hassankhan, Davood A. Khaburi

Abstract:

Direct Torque Control is a control technique in AC drive systems to obtain high performance torque control. The conventional DTC drive contains a pair of hysteresis comparators. DTC drives utilizing hysteresis comparators suffer from high torque ripple and variable switching frequency. The most common solution to those problems is to use the space vector depends on the reference torque and flux. In this Paper The space vector modulation technique (SVPWM) is applied to 2 level inverter control in the proposed DTC-based induction motor drive system, thereby dramatically reducing the torque ripple. Then the controller based on space vector modulation is designed to be applied in the control of Induction Motor (IM) with a three-level Inverter. This type of Inverter has several advantages over the standard two-level VSI, such as a greater number of levels in the output voltage waveforms, Lower dV/dt, less harmonic distortion in voltage and current waveforms and lower switching frequencies. This paper proposes a general SVPWM algorithm for three-level based on standard two-level SVPWM. The proposed scheme is described clearly and simulation results are reported to demonstrate its effectiveness. The entire control scheme is implemented with Matlab/Simulink.

Keywords: Direct torque control, space vector Pulsewidthmodulation(SVPWM), neutral point clamped(NPC), two-levelinverter.

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Authors: M. Kasumović, A. Hodžić, M. Tešanović

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One way for optimum loading of overdimensioning conveyers is speed (capacity) decrement, with attention for production capabilities and demands. At conveyers which drives with three phase slip-ring induction motor, technically reasonable solution for conveyer (driving motors) speed regulation is using constant torque subsynchronous cascade with static semiconductor converter and transformer for energy reversion to the power network. In the paper is described mathematical model for parameter calculation of two-motors 6 kV subsynchronous cascade. It is also demonstrated that applying of this cascade gave several good properties, foremost in electrical energy saving, also in improving of other energy indexes, and finally that results in cost reduction of complete electrical motor drive.

Keywords: Conveyer with rubber belt, electrical motor drive, sub synchronous cascade

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Authors: K. Djellabi, M. E. H. Latreche

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Induction heating computer simulation is a powerful tool for process design and optimization, induction coil design, equipment selection, as well as education and business presentations. The authors share their vast experience in the practical use of computer simulation for different induction heating and heat treating processes. In this paper treated with mathematical modeling and numerical simulation of induction heating furnaces with axisymmetric geometries for the numerical solution, we propose finite element methods combined with boundary (FEM) for the electromagnetic model using COMSOL® Multiphysics Software. Some numerical results for an industrial furnace are shown with high frequency.

Keywords: Numerical methods, Induction furnaces, Induction Heating, Finite element method, Comsol Multiphysics software.

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Authors: M. Ahmadi, M. Kafil, H. Ebrahimi

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Nowadays, induction motors have a significant role in industries. Condition monitoring (CM) of this equipment has gained a remarkable importance during recent years due to huge production losses, substantial imposed costs and increases in vulnerability, risk, and uncertainty levels. Motor current signature analysis (MCSA) is one of the most important techniques in CM. This method can be used for rotor broken bars detection. Signal processing methods such as Fast Fourier transformation (FFT), Wavelet transformation and Empirical Mode Decomposition (EMD) are used for analyzing MCSA output data. In this study, these signal processing methods are used for broken bar problem detection of Mobarakeh steel company induction motors. Based on wavelet transformation method, an index for fault detection, CF, is introduced which is the variation of maximum to the mean of wavelet transformation coefficients. We find that, in the broken bar condition, the amount of CF factor is greater than the healthy condition. Based on EMD method, the energy of intrinsic mode functions (IMF) is calculated and finds that when motor bars become broken the energy of IMFs increases.

Keywords: Broken bar, condition monitoring, diagnostics, empirical mode decomposition, Fourier transform, wavelet transform.

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Authors: Ilona I. Iatcheva, Rumena D. Stancheva, Mario Metodiev

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The paper deals with determination of electromagnetic and temperature field distribution of induction heating system used for pipe brazing. The problem is considered as coupled – time harmonic electromagnetic and transient thermal field. It has been solved using finite element method. The detailed maps of electromagnetic and thermal field distribution have been obtained. The good understanding of the processes in the considered system ensures possibilities for control, management and increasing the efficiency of the welding process.

Keywords: Electromagnetic field, temperature field, finiteelement method, induction heating, pipe brazing.

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Authors: Michel Han, Christophe Besson, Alain Savary, Yvan Becher

Abstract:

The variable flux permanent magnet synchronous motor (VF-PMSM), also called "Memory Motor", is a new generation of motor capable of modifying the magnetization state with short pulses of current during operation or standstill. The impact of such operation is the expansion of the operating range in the torque-speed characteristic and an improvement in energy efficiency at high-speed in comparison to conventional permanent magnet synchronous machines (PMSMs). This paper reviews the operating principle and the unique features of the proposed memory motor. The benefits of this concept are highlighted by comparing the performance of the rotor of the VF-PMSM to that of two PM rotors that are typically found in the industry. The investigation emphasizes the properties of the variable magnetization and presents the comparison of the torque-speed characteristic with the capability of loss reduction in a VF-PMSM by means of experimental results, especially when tests are conducted under identical conditions for each rotor (same stator, same inverter and same experimental setup). The experimental results demonstrated that the VF-PMSM gives an additional degree of freedom to optimize the efficiency over a wide speed range. Thus, with a design easy to manufacture and with the possibility of controlling the magnetization and the demagnetization of the magnets during operations, the VF-PMSM can be interesting for various applications.

Keywords: Efficiency, magnetization state, memory motors, performances, permanent-magnet, synchronous machine, variable-flux, variable magnetization, wide speed application.

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Authors: Jung-Moo Seo, Jung-Hwan Kim, Se-Hyun Rhyu, Jun-Hyuk Choi, In-Soung Jung,

Abstract:

Brushless DC motor with high torque density and slim topology for easy loading for robot system is proposed and manufactured. Electromagnetic design is executed by equivalent magnetic circuit model and numerical analysis. Manufactured motor is tested and verified characteristics comparing with conventional BLDC motor.

Keywords: Brushless DC motor, Robot joint module, Torque density, Pole/slot ratio

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Authors: Young-Seok Choi

Abstract:

Cerebellar ataxia is a steadily progressive neurodegenerative disease associated with loss of motor control, leaving patients unable to walk, talk, or perform activities of daily living. Direct motor instruction in cerebella ataxia patients has limited effectiveness, presumably because an inappropriate closed-loop cerebellar response to the inevitable observed error confounds motor learning mechanisms. Could the use of EEG based BCI provide advanced biofeedback to improve motor imagery and provide a “backdoor” to improving motor performance in ataxia patients? In order to determine the feasibility of using EEG-based BCI control in this population, we compare the ability to modulate mu-band power (8-12 Hz) by performing a cued motor imagery task in an ataxia patient and healthy control.

Keywords: Cerebellar ataxia, Electroencephalogram, brain-computer interface, motor imagery.

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Authors: Gábor Kovács

Abstract:

Requirement for pole-changing motors emerged at the very early times of asynchronous motor design. Different solutions have been elaborated and some of them are generally used. An alternative is the so called 3 Y/3 Y pole-changing winding. This paper deals with high power application of this solution. A complete and comprehensive study is introduced, including features and design guidelines. The method presented in this paper is especially suitable for pole numbers being close to each other. The study also reveals that the method is more advantageous then the existing solutions for high power motors with 1:3 pole ratio. Using this motor, a new and complete drive supply system has been proposed as most appropriate arrangement of high power main naval propulsion drive. Further, the method makes possible to extend the pole ratio to 1:6, 1:9, 1:12, etc. At the end, the proposal is further extended to the here so far missing 1:4, 1:5, 1:7 etc. pole ratios. A complete proposal for the theoretically infinite range has been given in this way.

Keywords: Induction motor, pole changing 3Y/3Y, pole phase modulation, pole changing 1:3, 1:4, 1:5, 1:6.

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Authors: Bo-Wun Huang, Jao-Hwa Kuang, J.-G. Tseng, Yan-De Wu

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An investigation of noise in a micro stepping motor is considered to study in this article. Because of the trend towards higher precision and more and more small 3C (including Computer, Communication and Consumer Electronics) products, the micro stepping motor is frequently used to drive the micro system or the other 3C products. Unfortunately, noise in a micro stepped motor is too large to accept by the customs. To depress the noise of a micro stepped motor, the dynamic characteristics in this system must be studied. In this article, a Visual Basic (VB) computer program speed controlled micro stepped motor in a digital camera is investigated. Karman KD2300-2S non-contract eddy current displacement sensor, probe microphone, and HP 35670A analyzer are employed to analyze the dynamic characteristics of vibration and noise in a motor. The vibration and noise measurement of different type of bearings and different treatment of coils are compared. The rotating components, bearings, coil, etc. of the motor play the important roles in producing vibration and noise. It is found that the noise will be depressed about 3~4 dB and 6~7 dB, when substitutes the copper bearing with plastic one and coats the motor coil with paraffin wax, respectively.

Keywords: micro motor, noise, vibration

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Authors: Marinka K. Baghdasaryan, Siranush M. Muradyan, Avgen A. Gasparyan

Abstract:

Analytical expressions of the current and angular errors, as well as the frequency characteristics of an induction converter describing the relation with its structural parameters, the core and winding characteristics are obtained. Based on estimation of the dependences obtained, a mathematical problem of parametric optimization is formulated which can successfully be used for investigating and diagnosing an induction converter.

Keywords: Induction converters, magnetic circuit material, current and angular errors, frequency response, mathematical formulation, structural parameters.

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Authors: Dursun M., Koc F., Ozbay H.

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In this study, a double-sided linear switched reluctance motor (LSRM) drive was investigated as an alternative actuator for vertical linear transportation applications such as a linear elevator door, hospital and subway doors which move linearly and where accurate position control and rapid response is requested. A prototype sliding elevator door that is focused on a home elevator with LSRMs is designed. The motor has 6/4 poles, 3 phases, 8A, 24V, 250 W and 250 N pull forces. Air gap between rotor and translator poles of the designed motor and phase coil-s ideal inductance profile are obtained in compliance with the geometric dimensions. Operation and switching sections as motor and generator has been determined from the inductance profile.

Keywords: Linear switched reluctance motor, sliding door, elevator door, linear motor design.

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Authors: Design of 3-Step Skew BLAC Motor for Better Performance in Electric Power Steering System

Abstract:

In Electric Power Steering (EPS), spoke type Brushless AC (BLAC) motors offer distinct advantages over other electric motor types in terms torque smoothness, reliability and efficiency. This paper deals with the shape optimization of spoke type BLAC motor, in order to reduce cogging torque. This paper examines 3 steps skewing rotor angle, optimizing rotor core edge and rotor overlap length for reducing cogging torque in spoke type BLAC motor. The methods were applied to existing machine designs and their performance was calculated using finite- element analysis (FEA). Prototypes of the machine designs were constructed and experimental results obtained. It is shown that the FEA predicted the cogging torque to be nearly reduce using those methods.

Keywords: EPS, 3-Step skewing, spoke type BLAC, cogging torque, FEA, optimization.

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Authors: Chao Yi, Cunyue Lu, Lingwei Quan

Abstract:

Piezoelectric linear motors have the characteristics of great electromagnetic compatibility, high positioning accuracy, compact structure and no deceleration mechanism, which make it promising to applicate in micro-miniature precision drive systems. However, most piezoelectric motors are employed by flexible clamping, which has insufficient rigidity and is difficult to use in rapid positioning. Another problem is that this clamping method seriously affects the vibration efficiency of the vibrating unit. In order to solve these problems, this paper proposes a piezoelectric stack linear motor based on double-end rigid clamping. First, a piezoelectric linear motor with a length of only 35.5 mm is designed. This motor is mainly composed of a motor stator, a driving foot, a ceramic friction strip, a linear guide, a pre-tightening mechanism and a base. This structure is much simpler and smaller than most similar motors, and it is easy to assemble as well as to realize precise control. In addition, the properties of piezoelectric stack are reviewed and in order to obtain the elliptic motion trajectory of the driving head, a driving scheme of the longitudinal-shear composite stack is innovatively proposed. Finally, impedance analysis and speed performance testing were performed on the piezoelectric linear motor prototype. The motor can measure speed up to 25.5 mm/s under the excitation of signal voltage of 120 V and frequency of 390 Hz. The result shows that the proposed piezoelectric stacked linear motor obtains great performance. It can run smoothly in a large speed range, which is suitable for various precision control in medical images, aerospace, precision machinery and many other fields.

Keywords: Elliptical trajectory, linear motor, piezoelectric stack, rigid clamping.

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Authors: Imane El Karaoui, Mohammed Maaroufi, Hamid Chaikhy

Abstract:

Doubly Fed Induction Generator (DFIG) is one of the most reliable wind generator. Major problem in wind power generation is to generate Sinusoidal signal with very low THD on variable speed caused by inverter two levels used. This paper presents a multi-level inverter whose objective is to reduce the THD and the dimensions of the output filter. This work proposes a three-level NPC-type inverter, the results simulation are presented demonstrating the efficiency of the proposed inverter.

Keywords: DFIG, multilevel inverter, NPC inverter , THD, Induction machine.

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Authors: Nelson K. Lujara

Abstract:

The paper presents a practical three-phase PWM inverter suitable for low voltage, low rating energy efficient systems. The work in the paper is conducted with the view to establishing the significance of the loss contribution from the PWM inverter in the determination of the complete losses of a photovoltaic (PV) arraypowered induction motor drive water pumping system. Losses investigated include; conduction and switching loss of the devices and gate drive losses. It is found that the PWM inverter operates at a reasonable variable efficiency that does not fall below 92% depending on the load. The results between the simulated and experimental results for the system with or without a maximum power tracker (MPT) compares very well, within an acceptable range of 2% margin.

Keywords: Energy, Inverter, Losses, Photovoltaic.

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Authors: S. Poonsawat, T. Kulworawanichpong

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This paper presents the speed regulation scheme of a small brushless dc motor (BLDC motor) with trapezoidal back-emf consideration. The proposed control strategy uses the proportional controller in which the proportional gain, kp, is appropriately adjusted by using genetic algorithms. As a result, the proportional control can perform well in order to compensate the BLDC motor with load disturbance. This confirms that the proposed speed regulation scheme gives satisfactory results.

Keywords: BLDC motor, proportional controller, genetic algorithms.

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Authors: M. Ektesabi

Abstract:

In this paper the problems associated with immunity of embedded systems used in Motor-Drive systems are investigated and appropriate solutions are presented. Integration of VSD motor systems (Integral Motor) while partially reducing some of these effects, adds to immunity problem of their embedded systems. Fail safe operation of an Integral Motor in arduous industrial environments is considered. In this paper an integral motor with a unique design is proposed to overcome critical issues such as heat, vibration and electromagnetic interference which are damaging to sensitive electronics without requirement of any additional cooling system. Advantages of the proposed Integral motor are compactness of combo motor and drive system with no external cabling/wiring. This motor provides a perfect shielding for least amount of radiated emission. It has an inbuilt filter for EMC compliance and has been designed to provide lower EMC noise for immunity of the internal electronics as well as the other neighbouring systems.

Keywords: Electromagnetic Interference, Immunity, IntegralMotor, Radiated & Conducted Emission, Sensitive Electronics, Variable Speed Drive

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Authors: Cheng-Chi Yu, Chi-Shiun Chiou

Abstract:

This study developed a plug-in hybrid powertrain system which consisted of two continuous variable transmissions. By matching between the engine, motor, generator, and dual continuous variable transmissions, this integrated power system can take advantages of the components. The hybrid vehicle can be driven by the internal combustion engine, or electric motor alone, or by these two power sources together when the vehicle is driven in hard acceleration or high load. The energy management of this integrated hybrid system controls the power systems based on rule-based control strategy to achieve better fuel economy. When the vehicle driving power demand is low, the internal combustion engine is operating in the low efficiency region, so the internal combustion engine is shut down, and the vehicle is driven by motor only. When the vehicle driving power demand is high, internal combustion engine would operate in the high efficiency region; then the vehicle could be driven by internal combustion engine. This strategy would operate internal combustion engine only in optimal efficiency region to improve the fuel economy. In this research, the vehicle simulation model was built in MATLAB/ Simulink environment. The analysis results showed that the power coupled efficiency of the hybrid powertrain system with dual continuous variable transmissions was better than that of the Honda hybrid system on the market.

Keywords: Plug-in hybrid power system, fuel economy, performance, continuous variable transmission.

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Authors: N.H. Mvungi

Abstract:

This paper addresses control of commutation of switched reluctance (SR) motor without the use of a physical position detector. Rotor position detection schemes for SR motor based on magnetisation characteristics of the motor use normal excitation or applied current /voltage pulses. The resulting schemes are referred to as passive or active methods respectively. The research effort is in realizing an economical sensorless SR rotor position detector that is accurate, reliable and robust to suit a particular application. An effective and reliable means of generating commutation signals of an SR motor based on inductance profile of its stator windings determined using active probing technique is presented. The scheme has been validated online using a 4-phase 8/6 SR motor and an 8-bit processor.

Keywords: Position detection, rotor position, sensorless, switched reluctance, SR.

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Authors: H. Sediki, S. Djennoune

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The switching lag-time and the voltage drop across the power devices cause serious waveform distortions and fundamental voltage drop in pulse width-modulated inverter output. These phenomenons are conspicuous when both the output frequency and voltage are low. To estimate the output voltage from the PWM reference signal it is essential to take account of these imperfections and to correct them. In this paper, on-line compensation method is presented. It needs three simple blocs to add at the ideal reference voltages. This method does not require any additional hardware circuit and off- line experimental measurement. The paper includes experimental results to demonstrate the validity of the proposed method. It is applied, finally, in case of indirect vector controlled induction machine and implemented using dSpace card.

Keywords: Dead time, field-oriented control, Induction motor, PWM inverter, voltage drop.

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Authors: S. M. Shahinul Islam, Israt Ara, Narendra Tuteja, Sreeramanan Subramaniam

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Through anther and microspore culture methods, complete homozygous plants can be produced within a year as compared to the long inbreeding method. Isolated microspore culture is one of the most important techniques for rapid development of haploid plants. The efficiency of this method is influenced by several factors such as cultural conditions, growth regulators, plant media, pretreatments, physical and growth conditions of the donor plants, pollen isolation procedure, etc. The main purpose of this study was to improve the isolated microspore culture protocol in order to increase the efficiency of embryoids, its regeneration and reducing albinisms. Under this study we have tested mainly three different microspore isolation procedures by glass rod, homozeniger and by blending and found the efficiency on gametic embryogenesis. There are three types of media viz. washing, pre-culture and induction was used. The induction medium as AMC (modified MS) supplemented by 2, 4-D (2.5 mg/l), kinetin (0.5 mg/l) and higher amount of D-Manitol (90 g/l) instead of sucrose and two types of amino acids (L-glutamine and L-serine) were used. Out of three main microspore isolation procedure by homogenizer isolation (P₄) showed best performance on ELS induction (177%) and green plantlets (104%) compared with other techniques. For all cases albinisims occurred but microspore isolation from excised anthers by glass rod and homogenizer showed lesser numbers of albino plants that was also one of the important findings in this study.

Keywords: Androgenesis, pretreatment, microspore culture, regeneration, albino plants, Oryza sativa.

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