Search results for: Experimental Evaluation

Authors: Noriyoshi Hosoya, Kayoko Yamamoto

Abstract:

This study, focusing on the importance of encouraging outdoor activities for children, aims to propose and implement a Web-GIS based outdoor education program for junior high schools, which will then be evaluated by users. Specifically, for the purpose of improved outdoor activities in the junior high school education, the outdoor education program, with chiefly using the Web-GIS that provides a good information provision and sharing tool, is proposed and implemented before being evaluated by users. The conclusion of this study can be summarized in the following two points. (1) A five -step outdoor education program based on Web-GIS was proposed for a “second school" at junior high schools that was then implemented before being evaluated by teachers as users. (2) Based on the results of evaluation by teachers, it was clear that the general operation of Web-GIS based outdoor education program with them only is difficult due to their lack of knowledge regarding Web-GIS and that support staff who can effectively utilize Web-GIS are essential.

Keywords: Outdoor Education, Web-Based GIS (Geographical Information Systems), Junior High Schools, School Education

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Authors: M. Jändel

Abstract:

A new conceptual architecture for low-level neural pattern recognition is presented. The key ideas are that the brain implements support vector machines and that support vectors are represented as memory patterns in competitive queuing memories. A binary classifier is built from two competitive queuing memories holding positive and negative valence training examples respectively. The support vector machine classification function is calculated in synchronized evaluation cycles. The kernel is computed by bisymmetric feed-forward networks feed by sensory input and by competitive queuing memories traversing the complete sequence of support vectors. Temporary summation generates the output classification. It is speculated that perception apparatus in the brain reuses structures that have evolved for enabling fluent execution of prepared action sequences so that pattern recognition is built on internalized motor programmes.

Keywords: Competitive queuing model, Olfactory system, Pattern recognition, Support vector machine, Thalamus

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Authors: M. B. Kime, J. Ntambwe, J. Mwamba

Abstract:

Froth flotation remains to date as one of the most used metallurgical processes for concentrating metal-bearing minerals in ores. Oxide ores are relatively less amenable to froth flotation and require a judicious choice of reagents for the recovery of metals to be optimised. Laboratory batch flotation tests were conducted to determine the effect of two types of gasoil-rinkalore mixtures on the flotation response of a copper cobalt oxide ore sample. The head assay conducted on the initial ore sample showed that it contained about 2.90% of Cu, 0.12% of Co. Upon the flotation test work, the results obtained indicated that the concentrate obtained with use of the mixture gazoil-rinkalore RX yielded 8.24% Cu and 0.22% Co concentrate grades with recoveries of 76.0% Cu and 78.0% Co respectively. But, the concentrate obtained by use of the mixture gazoil-rinkalore RX3 yielded relatively bad results with 5.92% Cu and 0.18% Cu concentrate grades with recoveries of 70.3% Cu and 65.3% Co respectively.

Keywords: Cobalt, copper, froth flotation, Rinkalore RX, Rinkalore RX3, Shangolowe.

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Authors: Adriana Alexandru, Cristian Tantareanu, Elena Jitaru

Abstract:

The paper presents the potential for RES in Romania and the results of the Romanian national research project “Romania contribution to the European targets regarding the development of renewable energy sources - PROMES". The objective of the project is the development of energy generation from renewable energy sources (RES) in Romania by drawing up scenarios and prognosis harmonized with national and European targets, RES development effects modeling (environmental, economic, social etc.), research of the impact of the penetration of RES into the main, implementation of an advanced software system tool for RES information recording and communication, experimental research based on demonstrative applications. The expected results are briefly presented, as well as the social, economic and environmental impact.

Keywords: Energy policy, Internet, renewable energy sources, potential.

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Authors: Fatima Setti, Ezziane Karim, Setti Bakhti, Negadi Kheira

Abstract:

The objective of this study is to present and to analyze the feasibility of using steel fibers as reinforcement in the cementations matrix to minimize the effect of free shrinkage which is a major cause of cracks that have can observe on concrete structures, also to improve the mechanical resistances of this concrete reinforced. The experimental study was performed on specimens with geometric characteristics adapted to the testing. The tests of shrinkage apply on prismatic specimens, equipped with rods fixed to the ends with different dosages of fibers, it should be noted that the fibers used are hooked end of 50mm length and 67 slenderness. The results show that the compressive strength and flexural strength increases as the degree of incorporation of fibbers increases. And the shrinkage deformations are generally less important for fibers-reinforced concrete to those appearing in the concrete without fibers.

Keywords: Concrete, Steel fibers, Compression, Flexural, Deformation.

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Authors: Babajide O. Ogungbesan, Rajneesh Kumar, Mohamed Sassi

Abstract:

Hydrogen sulfide (H2S) is a very toxic gas that is produced in very large quantities in the oil and gas industry. It cannot be flared to the atmosphere and Claus process based gas plants are used to recover the sulfur and convert the hydrogen to water. In this paper, we present optical characterization of an atmospheric pressure microwave plasma torch for H2S dissociation into hydrogen and sulfur. The torch is operated at 2.45 GHz with power up to 2 kW. Three different gases can simultaneously be injected in the plasma torch. Visual imaging and optical emission spectroscopy are used to characterize the plasma for varying gas flow rates and microwave power. The plasma length, emission spectra and temperature are presented. The obtained experimental results validate our earlier published simulation results of plasma torch.

Keywords: Atmospheric pressure microwave plasma, gas dissociation, optical emission spectroscopy.

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Authors: A. Benyahia, M. Zergoug, M. Amir, M. Fodil

Abstract:

The main objective of this work is to enhance the Pulsed Eddy Current (PEC) response from the aluminum structure using signal processing. Cracks and metal loss in different structures cause changes in PEC response measurements. In this paper, time-frequency analysis is used to represent PEC response, which generates a large quantity of data and reduce the noise due to measurement. Power Spectral Density (PSD) after Wavelet Decomposition (PSD-WD) is proposed for defect detection. The experimental results demonstrate that the cracks in the surface can be extracted satisfactorily by the proposed methods. The validity of the proposed method is discussed.

Keywords: NDT, pulsed eddy current, continuous wavelet transform, Mexican hat wavelet mother, defect detection, power spectral density.

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Authors: Mohamed A. Ahmed, Lydia J. Campbell

Abstract:

The fortified of soft wheat flour with cowpea flour in bread making was investigated. The Soft wheat flour (SWF) was substituted by cowpea flour at levels of 5, 15 and 20%. The protein content of composite breads ranged from 6.1 – 9.9%. Significant difference was observed in moisture, protein and crude fibre contents of control (wheat bread) and composite bread at 5% addition of cowpea. Water absorption capacities of composite flours increased with increasing levels of cowpea flour in the blend. The specific loaf volume decreased significantly with increased cowpea content of blends. The overall acceptability of the 5% cowpea flour content of composite bread was not significantly different from the control (Soft Wheat-bread) but there is significantly different with increasing the levels of cowpea flour in the blend more than 5%.

Keywords: Cowpea flour, wheat flour, baking properties, sensory quality.

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Authors: Abuzar.Abazari, Saeed. Ziaei-Rad, Hoseein. Dalayeli

Abstract:

Collision is considered as a time-depended nonlinear dynamic phenomenon. The majority of researchers have focused on deriving the resultant damage of the ship collisions via analytical, experimental, and finite element methods.In this paper, first, the force-penetration curve of a head collision on a container ship with rigid barrier based on Yang and Pedersen-s methods for internal mechanic section is studied. Next, the obtained results from different analytical methods are compared with each others. Then, through a simulation of the container ship collision in Ansys Ls-Dyna, results from finite element approach are compared with analytical methods and the source of errors is discussed. Finally, the effects of parameters such as velocity, and angle of collision on the forcepenetration curve are investigated.

Keywords: Ship collision, Force-penetration curve, Damage

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Authors: Pei-Syuan Ruan, Ya-Chi Yu, Shao-Wen Chen, Jin-Der Lee, Jong-Rong Wang, Chunkuan Shih

Abstract:

This study investigated the void fraction characteristics under low superficial gas velocity (J_g) and low superficial fluid velocity (J_f) conditions in a 3x3 rod bundle geometry. Three arrangements of conductivity probes were set to measure the void fraction at various cross-sectional regions, including rod-gap, sub-channel and rod-wall regions. The experimental tests were performed under the flow conditions of J_g = 0-0.236 m/s and J_f = 0-0.142 m/s, and the time-averaged void fractions were recorded at each flow condition. It was observed that while the superficial gas velocity increases, the small bubbles started to cluster together and become big bubbles. As the superficial fluid velocity increases, the local void fractions of the three test regions will get closer and the bubble distribution will be more uniform across the cross section.

Keywords: Conductivity probes, rod bundles, two-phase flow, void fraction.

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Authors: Elisabeth Brandenburg, Robert Woll, Rainer Stark

Abstract:

This paper presents how smart glasses and voice commands can be used for improving the maintenance process of industrial gas turbines. It presents the process of inspecting a gas turbine’s combustion chamber and how it is currently performed using a set of paper-based documents. In order to improve this process, a portable hands-free process assistance system has been conceived. In the following, it will be presented how the approach of user-centered design and the method of paper prototyping have been successfully applied in order to design a user interface and a corresponding workflow model that describes the possible interaction patterns between the user and the interface. The presented evaluation of these results suggests that the assistance system could help the user by rendering multiple manual activities obsolete, thus allowing him to work hands-free and to save time for generating protocols.

Keywords: Paper prototyping, smart glasses, turbine maintenance, user centered design.

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Authors: Ayman M. Alaskari, Samy E. Oraby, Abdulla I. Almazrouee

Abstract:

Coated tool inserts can be considered as the backbone of machining processes due to their wear and heat resistance. However, defects of coating can degrade the integrity of these inserts and the number of these defects should be minimized or eliminated if possible. Recently, the advancement of coating processes and analytical tools open a new era for optimizing the coating tools. First, an overview is given regarding coating technology for cutting tool inserts. Testing techniques for coating layers properties, as well as the various coating defects and their assessment are also surveyed. Second, it is introduced an experimental approach to examine the possible coating defects and flaws of worn multicoated carbide inserts using two important techniques namely scanning electron microscopy and atomic force microscopy. Finally, it is recommended a simple procedure for investigating manufacturing defects and flaws of worn inserts.

Keywords: AFM, Coated inserts, Defects, SEM.

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Authors: E. Shahbazali, N. Afrasiabi, A. A. Safekordi

Abstract:

Nowadays, fluidized bed plays an important part in industry. The design of this kind of reactor requires knowing the interfacial area between two phases and this interfacial area leads to calculate the solid holdup in the bed. Consequently achieving interfacial area between gas and solid in the bed experimentally is so significant. On interfacial area measurement in fluidized bed with gas has been worked, but light transmission technique has been used less. Therefore, in the current research the possibility of using of this technique and its accuracy are investigated. Measuring, a fluidized bed was designed and the problems were averted as far as possible. By using fine solid with equal shape and diameter and installing an optical system, the absorption of light during the time of fluidization has been measured. Results indicate that this method that its validity has been proved in the gas-liquid system, by different reasons have less application in gas-solid system. One important reason could be non-uniformity in such systems.

Keywords: Fluidization, Holdup, Light Transmission, Two phase system.

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Authors: T. Kosáros, D. Gál, F. Pekár, Gy. Lakatos

Abstract:

Periphyton development and composition were studied in three different treatments: (i) two fishpond units of wetland-type wastewater treatment pond systems, (ii) two fishponds in combined intensive-extensive fish farming systems and (iii) three traditional polyculture fishponds. Results showed that amounts of periphyton developed in traditional polyculture fishponds (iii) were different compared to the other treatments (i and ii), where the main function of ponds was stated wastewater treatment. Negative correlation was also observable between water quality parameters and periphyton production. The lower trophity, halobity and saprobity level of ponds indicated higher amount of periphyton. The dry matter content of periphyton was significantly higher in the samples, which were developed in traditional polyculture fishponds (2.84±3.02 g m-2 day-1, whereby the ash content in dry matter 74%), than samples taken from (i) (1.60±2.32 g m-2 day-1, 61%) and (ii) fishponds (0.65±0.45 g m-2 day-1, 81%).

Keywords: Artificial substrate, fishpond, periphyton, waterquality

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Authors: Munendra Kumar, Deepak Singh

Abstract:

The present investigation aims to study the effect of channel bed slopes on energy dissipation across the different types of Piano Key Weir (PK weir or PKW) under the free-flow conditions in rigid rectangular channels. To this end, three different types (type-A, type-B, and type-C) of PKW models were tested and examined. To document and quantify this experimental investigation, a total of 270 tests were performed, including detailed observations of the flow field. The results show that the energy dissipation of all PKW models increases with the bed slopes and decreases with increasing the discharge over the weirs. In addition, the energy dissipation over the PKW varies significantly with the geometry of the weir. The type-A PKW has shown the highest energy dissipation than the other PKWs. As the bottom slope changed from Sb = 0% to 1.25%, the energy dissipation increased by about 8.5%, 9.1%, and 10.55% for type-A, type-B, and type-C, respectively.

Keywords: Piano key weir, bed slope, energy dissipation across PKW, free overfalls.

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Authors: Ghassan A. Abed, Mahamod Ismail, Samir I. Badrawi, Bayan M. Sabbar

Abstract:

Long Term Evolution Advanced (LTE-Advanced) LTE-Advanced is not new as a radio access technology, but it is an evolution of LTE to enhance the performance. This generation is the continuation of 3GPP-LTE (3GPP: 3rd Generation Partnership Project) and it is targeted for advanced development of the requirements of LTE in terms of throughput and coverage. The performance evaluation process of any network should be based on many models and simulations to investigate the network layers and functions and monitor the employment of the new technologies especially when this network includes large-bandwidth and low-latency links such as LTE and LTE-Advanced networks. Therefore, it’s necessary to enhance the proposed models of high-speed and high-congested link networks to make these links and traffics fulfill the needs of the huge data which transferred over the congested links. This article offered an innovative model of the most correlated links of LTE-Advanced system using the Network Simulator 2 (NS-2) with investigation of the link parameters.

Keywords: 3GPP, LTE, LTE-Advanced, NS-2.

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Authors: Ji Hyung Park, Dong-Hyun Seo, Young-Jin Jung, Han Sung Kim

Abstract:

The aim of this study is to evaluate the effects of the laser and partial vibration stimulation on the mice tibia with morphological characteristics. Twenty female C57BL/6 mice (12 weeks old) were used for the experiment. The study was carried out on four groups of animals each consisting of five mice. Four groups of mice were ovariectomized. Animals were scanned at 0 and 2 weeks after ovariectomy by using micro computed tomography to estimate morphological characteristics of tibial trabecular bone. Morphological analysis showed that structural parameters of multi-stimuli group appear significantly better phase in BV/TV, BS/BV, Tb.Th, Tb.N, Tb.Sp, and Tb.pf than single stimulation groups. However, single stimulation groups didn’t show significant effect on tibia with Sham group. This study suggests that multi-stimuli may restrain the change as the degenerate phase on osteoporosis in the mice tibia.

Keywords: Laser, Partial Vibration, Osteoporosis, in vivo micro-CT, mice.

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Authors: Hee-Choul Kwon, Heeyong Kwon

Abstract:

Image rotation is one of main pre-processing steps for image processing or image pattern recognition. It is implemented with a rotation matrix multiplication. It requires a lot of floating point arithmetic operations and trigonometric calculations, so it takes a long time to execute. Therefore, there has been a need for a high speed image rotation algorithm without two major time-consuming operations. However, the rotated image has a drawback, i.e. distortions. We solved the problem using an augmented two-step shear transform. We compare the presented algorithm with the conventional rotation with images of various sizes. Experimental results show that the presented algorithm is superior to the conventional rotation one.

Keywords: High speed rotation operation, image rotation, transform matrix, image processing, pattern recognition.

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Authors: Mehmet Altınöz

Abstract:

Today, the working areas put forward the administration of change. In order to provide this; it is required from the organizations to be creative. Professional creativity in offices depends on an environment that enables the development of the organization only after the individual or collective exertions within the organization. By providing this environment, the organization will gain efficiency, productivity, and work pleasure. In order to bring up the workforce appropriate to the related expectations, the professional creativity of the office management and secretarial profession candidates should be evaluated, education programs appropriate to this and related directly with the service quality should be prepared and the future of this profession should be directed. The aim of this study is to ensure the attention to improve the prepared education program as well as the creative thoughts and their applications, when carrying out an office management and secretarial training. 144 students took place in this research and a questionnaire of 48 questions was carried out.

Keywords: Creativity, professional creativity, creativity evaluation, office management, secretarial

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Authors: Shuhana Shamsuddin, Nur Rasyiqah Abu Hassan, Ahmad Bashri Sulaiman

Abstract:

Liveable city is referred to as the quality of life in an area that contributes towards a safe, healthy and enjoyable place. This paper discusses the role of the streets- activities in making Kuala Lumpur a liveable city and the happiness level of the residents towards the city-s street activities. The study was conducted using the residents of Kuala Lumpur. A mixed method technique is used with the quantitative data as a main data and supported by the qualitative data. Data were collected using questionnaires, observation and also an interview session with a sample of residents of Kuala Lumpur. The sampling technique is based on multistage cluster data sampling. The findings revealed that, there is still no significant relationship between the length of stay of the resident in Kuala Lumpur with the happiness level towards the street activities that occurred in the city.

Keywords: Liveable city, activities, urban design quality, quality of life, happiness level.

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Authors: Yang Weiping, Zhang Zhilong, Zhang Yan, Chen Zengping

Abstract:

Infrared focal plane arrays (IRFPA) sensors, due to their high sensitivity, high frame frequency and simple structure, have become the most prominently used detectors in military applications. However, they suffer from a common problem called the fixed pattern noise (FPN), which severely degrades image quality and limits the infrared imaging applications. Therefore, it is necessary to perform non-uniformity correction (NUC) on IR image. The algorithms of non-uniformity correction are classified into two main categories, the calibration-based and scene-based algorithms. There exist some shortcomings in both algorithms, hence a novel non-uniformity correction algorithm based on non-linear fit is proposed, which combines the advantages of the two algorithms. Experimental results show that the proposed algorithm acquires a good effect of NUC with a lower non-uniformity ratio.

Keywords: Non-uniformity correction, non-linear fit, two-point correction, temporal Kalman filter.

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Authors: H. Izakian, S. A. Monadjemi, B. Tork Ladani, K. Zamanifar

Abstract:

Nowadays, OCR systems have got several applications and are increasingly employed in daily life. Much research has been done regarding the identification of Latin, Japanese, and Chinese characters. However, very little investigation has been performed regarding Farsi/Arabic characters recognition. Probably the reason is difficulty and complexity of those characters identification compared to the others and limitation of IT activities in Farsi and Arabic speaking countries. In this paper, a technique has been employed to identify isolated Farsi/Arabic characters. A chain code based algorithm along with other significant peculiarities such as number and location of dots and auxiliary parts, and the number of holes existing in the isolated character has been used in this study to identify Farsi/Arabic characters. Experimental results show the relatively high accuracy of the method developed when it is tested on several standard Farsi fonts.

Keywords: Farsi characters, OCR, feature extraction, chain code.

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Authors: Abdulsalam A. Al-Sudairi

Abstract:

Object-oriented simulation is considered one of the most sophisticated techniques that has been widely used in planning, designing, executing and maintaining construction projects. This technique enables the modeler to focus on objects which is extremely important for thorough understanding of a system. Thus, identifying an object is an essential point of building a successful simulation model. In a maintenance process an object is a maintenance work order (MWO). This study demonstrates a maintenance simulation model for the building maintenance division of Saudi Consolidated Electric Company (SCECO) in Dammam, Saudi Arabia. The model focused on both types of maintenance processes namely: (1) preventive maintenance (PM) and (2) corrective maintenance (CM). It is apparent from the findings that object-oriented simulation is a good diagnostic and experimental tool. This is because problems, limitations, bottlenecks and so forth are easily identified. These features are very difficult to obtain when using other tools.

Keywords: Object oriented, simulation, maintenance, process, work orders

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Authors: Jianqin Qu

Abstract:

This paper proposes a rigid point set matching algorithm in arbitrary dimensions based on the idea of symmetric covariant function. A group of functions of the points in the set are formulated using rigid invariants. Each of these functions computes a pair of correspondence from the given point set. Then the computed correspondences are used to recover the unknown rigid transform parameters. Each computed point can be geometrically interpreted as the weighted mean center of the point set. The algorithm is compact, fast, and dimension free without any optimization process. It either computes the desired transform for noiseless data in linear time, or fails quickly in exceptional cases. Experimental results for synthetic data and 2D/3D real data are provided, which demonstrate potential applications of the algorithm to a wide range of problems.

Keywords: Covariant point, point matching, dimension free, rigid registration.

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Authors: Sang-Soo Kim, Seong-Bae Park, Sang-Jo Lee

Abstract:

In this paper, we propose a method of resolving dependency ambiguities of Korean subordinate clauses based on Support Vector Machines (SVMs). Dependency analysis of clauses is well known to be one of the most difficult tasks in parsing sentences, especially in Korean. In order to solve this problem, we assume that the dependency relation of Korean subordinate clauses is the dependency relation among verb phrase, verb and endings in the clauses. As a result, this problem is represented as a binary classification task. In order to apply SVMs to this problem, we selected two kinds of features: static and dynamic features. The experimental results on STEP2000 corpus show that our system achieves the accuracy of 73.5%.

Keywords: Dependency analysis, subordinate clauses, binaryclassification, support vector machines.

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Authors: Tao Quang Bang, Insu Jeon

Abstract:

Most CT reconstruction system x-ray computed tomography (CT) is a well established visualization technique in medicine and nondestructive testing. However, since CT scanning requires sampling of radiographic projections from different viewing angles, common CT systems with mechanically moving parts are too slow for dynamic imaging, for instance of multiphase flows or live animals. A large number of X-ray projections are needed to reconstruct CT images, so the collection and calculation of the projection data consume too much time and harmful for patient. For the purpose of solving the problem, in this study, we proposed a method for tomographic reconstruction of a sample from a limited number of x-ray projections by using linear interpolation method. In simulation, we presented reconstruction from an experimental x-ray CT scan of a Aluminum phantom that follows to two steps: X-ray projections will be interpolated using linear interpolation method and using it for CT reconstruction based upon Ordered Subsets Expectation Maximization (OSEM) method.

Keywords: CT reconstruction, X-ray projections, Interpolation technique, OSEM

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Authors: Shinji Kajiwara

Abstract:

The spring-driven ball-type check valve is one of the most important components of hydraulic systems: it controls the position of the ball and prevents backward flow. To simplify the structure, the spring must be eliminated, and to accomplish this, the flow pattern and the behavior of the check ball in L-shaped pipe must be determined. In this paper, we present a full-scale model of a check ball made of acrylic resin, and we determine the relationship between the initial position of the ball, the position and diameter of the inflow port. The check flow rate increases in a standard center inflow model, and it is possible to greatly decrease the check-flow rate by shifting the inflow from the center.

Keywords: Hydraulics, Pipe Flow, Numerical Simulation, Flow Visualization, Check ball, L-shaped Pipe.

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Authors: Chung-Chih Lin, Wen-Teng Wang, Chin-Chiuan Kuo, Chieh-Liang Wu

Abstract:

The state of melt viscosity in injection process is significantly influenced by the setting parameters due to that the shear rate of injection process is higher than other processes. How to determine plastic melt viscosity during injection process is important to understand the influence of setting parameters on the melt viscosity. An apparatus named as pressure sensor bushing (PSB) module that is used to evaluate the melt viscosity during injection process is developed in this work. The formulations to coupling melt viscosity with fill time and injection pressure are derived and then the melt viscosity is determined. A test mold is prepared to evaluate the accuracy on viscosity calculations between the PSB module and the conventional approaches. The influence of melt viscosity on the tensile strength of molded part is proposed to study the consistency of injection quality.

Keywords: Injection molding, melt viscosity, injection quality, injection speed.

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Authors: M. Keshlaf, R. Spooner-Hart

Abstract:

Australia does not have varroa mite. However, we investigated whether modified hive bottom boards used for varroa mite management in honey bee colonies had other benefits, for honey production. We compared a number of colony parameters between hives fitted with tube, mesh and conventional (solid) bottom boards in two locations in eastern Australian, Richmond NSW and Castlemaine Victoria. Colonies housed in hives with mesh and tube bottom boards were not significantly superior to those in hives with conventional bottom boards with regard to bee flight activity, nor did they produce more honey, brood or stored pollen, in either experimental site. Although the trial was conducted over only one season, it is suggested that there may be no benefit in Australian bee keepers changing from using conventional bottom boards in the absence of varroamite.

Keywords: Apis mellifera, honey production, mesh bottom boards, tube bottom boards.

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Authors: Hrabe Thomas, Beck Florian, Nickell Stephan

Abstract:

Cryo-electron microscopy (CEM) in combination with single particle analysis (SPA) is a widely used technique for elucidating structural details of macromolecular assemblies at closeto- atomic resolutions. However, development of automated software for SPA processing is still vital since thousands to millions of individual particle images need to be processed. Here, we present our workflow for automated particle picking. Our approach integrates peak shape analysis to the classical correlation and an iterative approach to separate macromolecules and background by classification. This particle selection workflow furthermore provides a robust means for SPA with little user interaction. Processing simulated and experimental data assesses performance of the presented tools.

Keywords: Cryo-electron Microscopy, Single Particle Analysis, Image Processing.

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