Search results for: Variable substrate thickness.
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1661

Search results for: Variable substrate thickness.

941 Analysis of Green Wood Preservation Chemicals

Authors: Aitor Barbero-López, Soumaya Chibily, Gerhard Scheepers, Thomas Grahn, Martti Venäläinen, Antti Haapala

Abstract:

Wood decay is addressed continuously within the wood industry through use and development of wood preservatives. The increasing awareness on the negative effects of many chemicals towards the environment is causing political restrictions in their use and creating more urgent need for research on green alternatives. This paper discusses some of the possible natural extracts for wood preserving applications and compares the analytical methods available for testing their behavior and efficiency against decay fungi. The results indicate that natural extracts have interesting chemical constituents that delay fungal growth but vary in efficiency depending on the chemical concentration and substrate used. Results also suggest that presence and redistribution of preservatives in wood during exposure trials can be assessed by spectral imaging methods although standardized methods are not available. This study concludes that, in addition to the many standard methods available, there is a need to develop new faster methods for screening potential preservative formulation while maintaining the comparability and relevance of results.

Keywords: Analytics, methods, preservatives, wood decay.

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940 Power Control in a Doubly Fed Induction Machine

Authors: A. Ourici

Abstract:

This paper proposes a direct power control for doubly-fed induction machine for variable speed wind power generation. It provides decoupled regulation of the primary side active and reactive power and it is suitable for both electric energy generation and drive applications. In order to control the power flowing between the stator of the DFIG and the network, a decoupled control of active and reactive power is synthesized using PI controllers.The obtained simulation results show the feasibility and the effectiveness of the suggested method

Keywords: Doubly fed induction machine , decoupled power control , vector control , active and reactive power, PWM inverter

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939 Ranking DMUs by Ideal PPS in Data Envelopment Analysis

Authors: V.Rezaie, M.Khanmohammady

Abstract:

An original DEA model is to evaluate each DMU optimistically, but the interval DEA Model proposed in this paper has been formulated to obtain an efficiency interval consisting of Evaluations from both the optimistic and the pessimistic view points. DMUs are improved so that their lower bounds become so large as to attain the maximum Value one. The points obtained by this method are called ideal points. Ideal PPS is calculated by ideal of efficiency DMUs. The purpose of this paper is to rank DMUs by this ideal PPS. Finally we extend the efficiency interval of a DMU under variable RTS technology.

Keywords: Data envelopment analysis (DEA), Decision makingunit (DMU), Interval DEA, Ideal points, Ideal PPS, Return to scale(RTS).

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938 Study on Bending Characteristics of Square Tube Using Energy Absorption Part

Authors: Shigeyuki Haruyama, Zefry Darmawan, Ken Kaminishi

Abstract:

In the square tube subjected to the bending load, the rigidity of the entire square tube is reduced when a collapse occurs due to local stress concentration. Therefore, in this research, the influence of bending load on the square tube with attached energy absorbing part was examined and reported. The analysis was conducted by using Finite Element Method (FEM) to produced bending deflection and buckling points. Energy absorption was compared from rigidity of attached part and square tube body. Buckling point was influenced by the rigidity of attached part and the thickness rate of square tube.

Keywords: Square tube, bending stress, energy absorption, finite element analysis, rigidity.

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937 Graphene/h-BN Heterostructure Interconnects

Authors: Nikhil Jain, Yang Xu, Bin Yu

Abstract:

The material behavior of graphene, a single layer of carbon lattice, is extremely sensitive to its dielectric environment. We demonstrate improvement in electronic performance of graphene nanowire interconnects with full encapsulation by lattice-matching, chemically inert, 2D layered insulator hexagonal boron nitride (h- BN). A novel layer-based transfer technique is developed to construct the h-BN/MLG/h-BN heterostructures. The encapsulated graphene wires are characterized and compared with that on SiO2 or h-BN substrate without passivating h-BN layer. Significant improvements in maximum current-carrying density, breakdown threshold, and power density in encapsulated graphene wires are observed. These critical improvements are achieved without compromising the carrier transport characteristics in graphene. Furthermore, graphene wires exhibit electrical behavior less insensitive to ambient conditions, as compared with the non-passivated ones. Overall, h-BN/graphene/h- BN heterostructure presents a robust material platform towards the implementation of high-speed carbon-based interconnects.

Keywords: Two-dimensional nanosheet, graphene, hexagonal boron nitride, heterostructure, interconnects.

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936 Three-Dimensional Numerical Investigation for Reinforced Concrete Slabs with Opening

Authors: Abdelrahman Elsehsah, Hany Madkour, Khalid Farah

Abstract:

This article presents a 3-D modified non-linear elastic model in the strain space. The Helmholtz free energy function is introduced with the existence of a dissipation potential surface in the space of thermodynamic conjugate forces. The constitutive equation and the damage evolution were derived as well. The modified damage has been examined to model the nonlinear behavior of reinforced concrete (RC) slabs with an opening. A parametric study with RC was carried out to investigate the impact of different factors on the behavior of RC slabs. These factors are the opening area, the opening shape, the place of opening, and the thickness of the slabs. And the numerical results have been compared with the experimental data from literature. Finally, the model showed its ability to be applied to the structural analysis of RC slabs.

Keywords: 3-D numerical analysis, damage mechanics, RC slab with opening.

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935 Study of Debonding of Composite Material from a Deforming Concrete Beam Using Infrared Thermography

Authors: Igor Shardakov, Anton Bykov, Alexey Shestakov, Irina Glot

Abstract:

This article focuses on the cycle of experimental studies of the formation of cracks and debondings in the concrete reinforced with carbon fiber. This research was carried out in Perm National Research Polytechnic University. A series of CFRP-strengthened RC beams was tested to investigate the influence of preload and crack repairing factors on CFRP debonding. IRT was applied to detect the early stage of IC debonding during the laboratory bending tests. It was found that for the beams strengthened under load after crack injecting, СFRP debonding strain is 4-65% lower than for the preliminary strengthened beams. The beams strengthened under the load had a relative area of debonding of 2 times higher than preliminary strengthened beams. The СFRP debonding strain is weakly dependent on the strength of the concrete substrate. For beams with a transverse wrapping anchorage in support sections FRP debonding is not a failure mode.

Keywords: FRP, RC beams, strengthening, IC debonding, infrared thermography, quality control, non-destructive testing methods.

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934 Fuzzy Processing of Uncertain Data

Authors: Petr Morávek, Miloš Šeda

Abstract:

In practice, we often come across situations where it is necessary to make decisions based on incomplete or uncertain data. In control systems it may be due to the unknown exact mathematical model, or its excessive complexity (e.g. nonlinearity) when it is necessary to simplify it, respectively, to solve it using a rule base. In the case of databases, searching data we compare a similarity measure with of the requirements of the selection with stored data, where both the select query and the data itself may contain vague terms, for example in the form of linguistic qualifiers. In this paper, we focus on the processing of uncertain data in databases and demonstrate it on the example multi-criteria decision making in the selection of variants, specified by higher number of technical parameters.

Keywords: fuzzy logic, linguistic variable, multicriteria decision

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933 Effects of Electric Potential on Thermo-Mechanical Behavior of Functionally Graded Piezoelectric Hollow Cylinder under Non-Axisymmetric Loads

Authors: Amir Atrian, Javad Jafari Fesharaki, Gh. Hossein Majzoobi, Mahsa Sheidaee

Abstract:

The analytical solution of functionally graded piezoelectric hollow cylinder which is under radial electric potential and non-axisymmetric thermo-mechanical loads, are presented in this paper. Using complex Fourier series and estimation of power law for variations of material characterizations through the thickness, the electro thermo mechanical behavior of the FGPM cylinder is obtained. The stress and displacement distributions and the effect of electric potential field on the cylinder behavior are also presented and some applicable results are offered at the end of the paper.

Keywords: Analytical, FGM, Fourier series, Non-axisymmetric, Piezoelectric, Thermo-elasticity

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932 Meandered Microstrip Open Circuited Stub with Bandstop Characteristic

Authors: Goh Chin Hock, Chandan Kumar Chakrabarty, Mohammad Hadi Badjian, Sanjay Devkumar

Abstract:

This paper presents a microstrip meandered open circuited stub with bandstop characteristic. The proposed structure is designed on a high frequency laminate with dielectric constant of 4.0 and board thickness of 0.508 millimeters. The scattering parameters and electromagnetic field distributions at various frequencies are investigated by modeling the structure with three dimensional electromagnetic simulation tool. In order to describe the resonant and bandstop characteristic of the meandered open circuited stub, a Smith chart as well as electric field at various frequencies and phases is illustrated accordingly. The structure can be an alternative method in suppressing the harmonic response of a bandpass filter.

Keywords: Bandstop, Equivalent Lumped Element Model, Electromagnetic Model, Meandered Open Circuited Stub

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931 Assessment of Using Wastage Steel as Welded Reinforcement

Authors: Muhammad Murtaza Nasir, Safdar Abbas Zaidi, Kamran Khan

Abstract:

This work is carried out to evaluate the possibility of using to-be-wasted steel as reinforcement after welding together pieces of reinforcing steel bars, left over during construction activities. Tests were performed on a total of nine samples. These were made by welding pieces of reinforcing steel bars purchased from the local scrap steel market. The samples were tested in uniaxial tension using a universal testing machine (UTM). It was found that the failure of the welded bars is governed by the thickness of the weld. It is concluded that suitable design of the weld is essential for achieving the desired level of ductility/elongation of these bars, if they are to be used as conventional reinforcement in reinforced concrete members.

Keywords: Ductility/elongation, low cost housing, reinforced concrete, welding, welded reinforcement, wastage steel.

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930 Linear Pocket Profile based Threshold Voltage Model for sub-100 nm n-MOSFET

Authors: Muhibul Haque Bhuyan, Quazi Deen Mohd Khosru

Abstract:

This paper presents a threshold voltage model of pocket implanted sub-100 nm n-MOSFETs incorporating the drain and substrate bias effects using two linear pocket profiles. Two linear equations are used to simulate the pocket profiles along the channel at the surface from the source and drain edges towards the center of the n-MOSFET. Then the effective doping concentration is derived and is used in the threshold voltage equation that is obtained by solving the Poisson-s equation in the depletion region at the surface. Simulated threshold voltages for various gate lengths fit well with the experimental data already published in the literature. The simulated result is compared with the two other pocket profiles used to derive the threshold voltage models of n-MOSFETs. The comparison shows that the linear model has a simple compact form that can be utilized to study and characterize the pocket implanted advanced ULSI devices.

Keywords: Linear pocket profile, pocket implantation, nMOSFET, threshold voltage, short channel effect (SCE), reverse short channeleffect (RSCE).

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929 Issues in Travel Demand Forecasting

Authors: Huey-Kuo Chen

Abstract:

Travel demand forecasting including four travel choices, i.e., trip generation, trip distribution, modal split and traffic assignment constructs the core of transportation planning. In its current application, travel demand forecasting has associated with three important issues, i.e., interface inconsistencies among four travel choices, inefficiency of commonly used solution algorithms, and undesirable multiple path solutions. In this paper, each of the three issues is extensively elaborated. An ideal unified framework for the combined model consisting of the four travel choices and variable demand functions is also suggested. Then, a few remarks are provided in the end of the paper

Keywords: Travel choices, B algorithm, entropy maximization, dynamic traffic assignment.

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928 Stress Concentration around Countersunk Hole in Isotropic Plate under Transverse Loading

Authors: Parveen K. Saini, Tarun Agarwal

Abstract:

An investigation into the effect of countersunk depth, plate thickness, countersunk angle and plate width on the stress concentration around countersunk hole is carried out with the help of finite element analysis. The variation of stress concentration with respect to these parameters is studied for three types of loading viz. uniformly distributed load, uniformly varying load and functionally distributed load. The results of the finite element analysis are interpreted and some conclusions are drawn. The distribution of stress concentration around countersunk hole in isotropic plates simply supported at all the edges is found similar and is independent of loading. The maximum stress concentration also occurs at a particular point irrespective of the loading conditions.

Keywords: Stress Concentration Factor, Countersunk hole, Finite element, ANSYS.

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927 Smith Predictor Design by CDM for Temperature Control System

Authors: Roengruen P., Tipsuwanporn V., Puawade P., Numsomran A.

Abstract:

Smith Predictor control is theoretically a good solution to the problem of controlling the time delay systems. However, it seldom gets use because it is almost impossible to find out a precise mathematical model of the practical system and very sensitive to uncertain system with variable time-delay. In this paper is concerned with a design method of smith predictor for temperature control system by Coefficient Diagram Method (CDM). The simulation results show that the control system with smith predictor design by CDM is stable and robust whilst giving the desired time domain system performance.

Keywords: CDM, Smith Predictor, temperature process

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926 Using Stresses Obtained from a Low Detailed FE Model and Located at a Reference Point to Quickly Calculate the Free-edge Stress Intensity Factors of Bonded Joints

Authors: F. Maamar, M. Sartor

Abstract:

The present study focuses on methods allowing a convenient and quick calculation of the SIFs in order to predict the static adhesive strength of bonded joints. A new SIF calculation method is proposed, based on the stresses obtained from a FE model at a reference point located in the adhesive layer at equal distance of the free-edge and of the two interfaces. It is shown that, even limiting ourselves to the two main modes, i.e. the opening and the shearing modes, and using the values of the stresses resulting from a low detailed FE model, an efficient calculation of the peeling stress at adhesive-substrate corners can be obtained by this way. The proposed method is interesting in that it can be the basis of a prediction tool that will allow the designer to quickly evaluate the SIFs characterizing a particular application without developing a detailed analysis.

Keywords: Adhesive layer, bounded joints, free-edge corner, stress intensity factor.

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925 Dye-Sensitized Solar Cell by Plasma Spray

Authors: C.C. Chen, C.C. Wei, S.H. Chen, S.J. Hsieh, W.G. Diau

Abstract:

This paper aims to scale up Dye-sensitized Solar Cell (DSSC) production using a commonly available industrial material – stainless steel - and industrial plasma equipment. A working DSSC electrode formed by (1) coating titania nanotube (TiO2 NT) film on 304 stainless steel substrate using a plasma spray technique; then, (2) filling the nano-pores of the TiO2 NT film using a TiF4 sol-gel method. A DSSC device consists of an anode absorbed photosensitive dye (N3), a transparent conductive cathode with platinum (Pt) nano-catalytic particles adhered to its surface, and an electrolytic solution sealed between the anode and the transparent conductive cathode. The photo-current conversion efficiency of the DSSC sample was tested under an AM 1.5 Solar Simulator. The sample has a short current (Isc) of 0.83 mA cm-2, open voltage (Voc) of 0.81V, filling factor (FF) of 0.52, and conversion efficiency (η) of 2.18% on a 0.16 cm2 DSSC work-piece.

Keywords: DSSC, Spray, stainless steel, TiO2 NT, efficiency

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924 In silico Studies on Selected Drug Targets for Combating Drug Resistance in Plasmodium falcifarum

Authors: D. Bhaskar, N. R. Wadehra, M. Gulati, A. Narula, R. Vishnu, G. Katyal

Abstract:

With drug resistance becoming widespread in Plasmodium falciparum infections, the development of the alternative drugs is the desired strategy for prevention and cure of malaria. Three drug targets were selected to screen promising drug molecules from the GSK library of 13469 molecules. Using an in silico structure-based drug designing approach, the differences in binding energies of the substrate and inhibitor were exploited between target sites of parasite and human to design a drug molecule against Plasmodium. The docking studies have shown several promising molecules from GSK library with more effective binding as compared to the already known inhibitors for the drug targets. Though stronger interaction has been shown by several molecules as compared to the reference, few molecules have shown the potential as drug candidates though in vitro studies are required to validate the results. In case of thymidylate synthase-dihydrofolatereductase (TS-DHFR), three compounds have shown promise for future studies as potential drugs.

Keywords: Drug resistance, Drug targets, In silico studies, Plasmodium falciparum.

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923 Application of Neural Network and Finite Element for Prediction the Limiting Drawing Ratio in Deep Drawing Process

Authors: H.Mohammadi Majd, M.Jalali Azizpour, A.V. Hoseini

Abstract:

In this paper back-propagation artificial neural network (BPANN) is employed to predict the limiting drawing ratio (LDR) of the deep drawing process. To prepare a training set for BPANN, some finite element simulations were carried out. die and punch radius, die arc radius, friction coefficient, thickness, yield strength of sheet and strain hardening exponent were used as the input data and the LDR as the specified output used in the training of neural network. As a result of the specified parameters, the program will be able to estimate the LDR for any new given condition. Comparing FEM and BPANN results, an acceptable correlation was found.

Keywords: Back-propagation artificial neural network(BPANN), deep drawing, prediction, limiting drawing ratio (LDR).

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922 Kalman Filter Based Adaptive Reduction of Motion Artifact from Photoplethysmographic Signal

Authors: S. Seyedtabaii, L. Seyedtabaii

Abstract:

Artifact free photoplethysmographic (PPG) signals are necessary for non-invasive estimation of oxygen saturation (SpO2) in arterial blood. Movement of a patient corrupts the PPGs with motion artifacts, resulting in large errors in the computation of Sp02. This paper presents a study on using Kalman Filter in an innovative way by modeling both the Artillery Blood Pressure (ABP) and the unwanted signal, additive motion artifact, to reduce motion artifacts from corrupted PPG signals. Simulation results show acceptable performance regarding LMS and variable step LMS, thus establishing the efficacy of the proposed method.

Keywords: Kalman filter, Motion artifact, PPG, Photoplethysmography.

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921 A Comparative Analysis of Financial Performance of Funded and Non-Funded Charity Organizations

Authors: Saunah Zainon, Ruhaya Atan, Yap Bee Wah, Zarina Abu Bakar

Abstract:

The primary objective of this study is to test whether there is any difference in performance between funded and nonfunded registered charity organizations. In this study, performance as the dependent variable is measured using total donations. Using a sample of 101 charity organizations registered with the Registry of Society, analysis of variance (ANOVA) results indicate that there is a difference in financial performance between funded and non-funded charity organizations. The study provides empirical evidence to resource providers and the policy makers in scrutinizing the decision to disburse their funds and resources to these charity organizations.

Keywords: charity organizations, donations, funded, non-funded

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920 Underwater Interaction of 1064 nm Laser Radiation with Metal Target

Authors: G. Toker, V. Bulatov, T. Kovalchuk, I. Schechter

Abstract:

Dynamics of laser radiation – metal target interaction in water at 1064 nm by applying Mach-Zehnder interference technique was studied. The mechanism of generating the well developed regime of evaporation of a metal surface and a spherical shock wave in water is proposed. Critical intensities of the NIR for the well developed evaporation of silver and gold targets were determined. Dynamics of shock waves was investigated for earlier (dozens) and later (hundreds) nanoseconds of time. Transparent expanding plasma-vapor-compressed water object was visualized and measured. The thickness of compressed layer of water and pressures behind the front of a shock wave for later time delays were obtained from the optical treatment of interferograms.

Keywords: laser, shock wave, metal target, underwater

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919 Analytical Solution of Stress Distribution ona Hollow Cylindrical Fiber of a Composite with Cylindrical Volume Element under Axial Loading

Authors: M. H. Kargarnovin, K. Momeni

Abstract:

The study of the stress distribution on a hollow cylindrical fiber placed in a composite material is considered in this work and an analytical solution for this stress distribution has been constructed. Finally some parameters such as fiber-s thickness and fiber-s length are considered and their effects on the distribution of stress have been investigated. For finding the governing relations, continuity equations for the axisymmetric problem in cylindrical coordinate (r,o,z) are considered. Then by assuming some conditions and solving the governing equations and applying the boundary conditions, an equation relates the stress applied to the representative volume element with the stress distribution on the fiber has been found.

Keywords: Axial Loading, Composite, Hollow CylindricalFiber, Stress Distribution.

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918 Synthesis of Monoacylglycerol from Glycerolysis of Crude Glycerol with Coconut Oil Catalyzed by Carica papaya Lipase

Authors: P. Pinyaphong, P. Sriburi, S. Phutrakul

Abstract:

This paper studied the synthesis of monoacylglycerol (monolaurin) by glycerolysis of coconut oil and crude glycerol, catalyzed by Carica papaya lipase. Coconut oil obtained from cold pressed extraction method and crude glycerol obtained from the biodiesel plant in Department of Chemistry, Uttaradit Rajabhat University, Thailand which used oils were used as raw materials for biodiesel production through transesterification process catalyzed by sodium hydroxide. The influences of the following variables were studied: (i) type of organic solvent, (ii) molar ratio of substrate, (iii) reaction temperature, (iv) reaction time, (v) lipase dosage, and (vi) initial water activity of enzyme. High yields in monoacylglycerol (58.35%) were obtained with molar ratio of glycerol to oil at 8:1 in ethanol, temperature was controlled at 45oC for 36 hours, the amount of enzyme used was 20 wt% of oil and initial water activity of enzyme at 0.53.

Keywords: Monoacylglycerol, crude glycerol, coconut oil, Carica papaya lipase.

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917 Prediction the Limiting Drawing Ratio in Deep Drawing Process by Back Propagation Artificial Neural Network

Authors: H.Mohammadi Majd, M.Jalali Azizpour, M. Goodarzi

Abstract:

In this paper back-propagation artificial neural network (BPANN) with Levenberg–Marquardt algorithm is employed to predict the limiting drawing ratio (LDR) of the deep drawing process. To prepare a training set for BPANN, some finite element simulations were carried out. die and punch radius, die arc radius, friction coefficient, thickness, yield strength of sheet and strain hardening exponent were used as the input data and the LDR as the specified output used in the training of neural network. As a result of the specified parameters, the program will be able to estimate the LDR for any new given condition. Comparing FEM and BPANN results, an acceptable correlation was found.

Keywords: BPANN, deep drawing, prediction, limiting drawingratio (LDR), Levenberg–Marquardt algorithm

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916 Optimal Solution of Constraint Satisfaction Problems

Authors: Jeffrey L. Duffany

Abstract:

An optimal solution for a large number of constraint satisfaction problems can be found using the technique of substitution and elimination of variables analogous to the technique that is used to solve systems of equations. A decision function f(A)=max(A2) is used to determine which variables to eliminate. The algorithm can be expressed in six lines and is remarkable in both its simplicity and its ability to find an optimal solution. However it is inefficient in that it needs to square the updated A matrix after each variable elimination. To overcome this inefficiency the algorithm is analyzed and it is shown that the A matrix only needs to be squared once at the first step of the algorithm and then incrementally updated for subsequent steps, resulting in significant improvement and an algorithm complexity of O(n3).

Keywords: Algorithm, complexity, constraint, np-complete.

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915 Design of Compact Dual-Band Planar Antenna for WLAN Systems

Authors: Anil Kumar Pandey

Abstract:

A compact planar monopole antenna with dual-band operation suitable for wireless local area network (WLAN) application is presented in this paper. The antenna occupies an overall area of 18 ×12 mm2. The antenna is fed by a coplanar waveguide (CPW) transmission line and it combines two folded strips, which radiates at 2.4 and 5.2 GHz. In the proposed antenna, by optimally selecting the antenna dimensions, dual-band resonant modes with a much wider impedance matching at the higher band can be produced. Prototypes of the obtained optimized design have been simulated using EM solver. The simulated results explore good dual-band operation with -10 dB impedance bandwidths of 50 MHz and 2400 MHz at bands of 2.4 and 5.2 GHz, respectively, which cover the 2.4/5.2/5.8 GHz WLAN operating bands. Good antenna performances such as radiation patterns and antenna gains over the operating bands have also been observed. The antenna with a compact size of 18×12×1.6 mm3 is designed on an FR4 substrate with a dielectric constant of 4.4.

Keywords: CPW fed antenna, dual-band, electromagnetic simulation, wireless local area network, WLAN.

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914 Optical Analysis of Variable Aperture Mechanism for a Solar Reactor

Authors: Akanksha Menon, Nesrin Ozalp

Abstract:

Solar energy is not only sustainable but also a clean alternative to be used as source of high temperature heat for many processes and power generation. However, the major drawback of solar energy is its transient nature. Especially in solar thermochemical processing, it is crucial to maintain constant or semiconstant temperatures inside the solar reactor. In our laboratory, we have developed a mechanism allowing us to achieve semi-constant temperature inside the solar reactor. In this paper, we introduce the concept along with some updated designs and provide the optical analysis of the concept under various incoming flux.

Keywords: Aperture, Solar reactor, Optical analysis, Solar thermal

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913 Green Synthesis of Butyl Acetate, A Pineapple Flavour via Lipase-Catalyzed Reaction

Authors: S. Mat Radzi, W.A.F. Mustafa, S.S Othman, H.M. Noor

Abstract:

Nowadays, butyl acetate, a pineapple flavor has been applied widely in food, beverage, cosmetic and pharmaceutical industries. In this study, Butyl acetate, a flavor ester was successfully synthesized via green synthesis of enzymatic reaction route. Commercial immobilized lipase from Rhizomucor miehei (Lipozyme RMIM) was used as biocatalyst in the esterification reaction between acetic acid and butanol. Various reaction parameters such as reaction time (RT), temperature (T) and amount of enzyme (E) were chosen to optimize the reaction synthesis in solvent-free system. The optimum condition to produce butyl acetate was at reaction time (RT), 18 hours; temperature (T), 37°C and amount of enzyme, 25 % (w/w of total substrate). Analysis of yield showed that at optimum condition, >78 % of butyl acetate was produced. The product was confirmed as butyl acetate from FTIR analysis whereby the presence of an ester group was observed at wavenumber of 1742 cm-1.

Keywords: Butyl acetate, immobilized enyzme, esterification, flavor ester, green synthesis

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912 Enhance Halorespiration in Rhodopseudomonas palustris with Cytochrome P450cam System from Pseudomonas putida

Authors: Shou-Chen Lo, Chia-Ching Lin, Chieh-Chen Huang

Abstract:

To decompose organochlorides by bioremediation, co-culture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78% after 16 hours, however, the cytochrome P450cam system expressed strain, CGA-camCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3-chlorobenzoate, as sole carbon source or present benzoate as co-substrate, CGA-camCAB presented faster growth rate than vector control strain.

Keywords: cytochrome P450, halorespiration, nitrogen fixation, Rhodopseudomonas palustris CGA009

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